Devosia geojensis
Average proteome isoelectric point is 6.22
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3992 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0F5FX53|A0A0F5FX53_9RHIZ Alanine racemase OS=Devosia geojensis OX=443610 GN=VE25_05870 PE=3 SV=1
MM1 pKa = 7.23 KK2 pKa = 8.8 TTILTLASLALLTGAATAQDD22 pKa = 4.25 TNPGATANFVDD33 pKa = 4.58 VEE35 pKa = 4.29 GNEE38 pKa = 4.01 IGAVTLLQTPEE49 pKa = 3.95 GVNITGALEE58 pKa = 3.88 MGVPEE63 pKa = 4.88 GEE65 pKa = 4.34 HH66 pKa = 6.31 GFHH69 pKa = 6.52 IHH71 pKa = 5.7 EE72 pKa = 5.18 TGDD75 pKa = 3.97 CDD77 pKa = 3.7 PADD80 pKa = 3.97 AFEE83 pKa = 4.39 SAGDD87 pKa = 3.7 HH88 pKa = 6.8 FDD90 pKa = 4.28 VADD93 pKa = 3.98 HH94 pKa = 5.87 QHH96 pKa = 6.49 GFDD99 pKa = 3.71 NPEE102 pKa = 4.0 GPHH105 pKa = 7.12 AGDD108 pKa = 3.0 IHH110 pKa = 7.9 NIIADD115 pKa = 3.54 ADD117 pKa = 3.48 GRR119 pKa = 11.84 AVVEE123 pKa = 4.29 VTNDD127 pKa = 3.15 MVSLVEE133 pKa = 5.05 GEE135 pKa = 4.33 DD136 pKa = 4.16 GYY138 pKa = 11.83 LFDD141 pKa = 6.48 DD142 pKa = 4.19 NGSALVVHH150 pKa = 7.44 ADD152 pKa = 3.25 PDD154 pKa = 4.2 DD155 pKa = 4.07 YY156 pKa = 11.72 VTDD159 pKa = 4.03 PSGNSGDD166 pKa = 4.54 RR167 pKa = 11.84 IACAVIEE174 pKa = 4.16 AAPNN178 pKa = 3.34
Molecular weight: 18.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.579
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.617
Grimsley 3.478
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.139
Thurlkill 3.63
EMBOSS 3.732
Sillero 3.91
Patrickios 1.888
IPC_peptide 3.77
IPC2_peptide 3.884
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|A0A0F5FRM7|A0A0F5FRM7_9RHIZ Heme exporter protein C OS=Devosia geojensis OX=443610 GN=ccmC PE=3 SV=1
MM1 pKa = 7.71 PATVTRR7 pKa = 11.84 PPPRR11 pKa = 11.84 ARR13 pKa = 11.84 WPRR16 pKa = 11.84 PAQRR20 pKa = 11.84 WPRR23 pKa = 11.84 RR24 pKa = 11.84 ALRR27 pKa = 11.84 YY28 pKa = 9.19 RR29 pKa = 11.84 ATSLRR34 pKa = 11.84 RR35 pKa = 11.84 TRR37 pKa = 11.84 PTPPALWPVSPPSRR51 pKa = 11.84 RR52 pKa = 11.84 VPRR55 pKa = 11.84 NPTSSRR61 pKa = 11.84 AMARR65 pKa = 11.84 ASTPP69 pKa = 3.03
Molecular weight: 7.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.497
IPC2_protein 11.272
IPC_protein 12.72
Toseland 12.881
ProMoST 13.378
Dawson 12.881
Bjellqvist 12.881
Wikipedia 13.349
Rodwell 12.384
Grimsley 12.91
Solomon 13.378
Lehninger 13.276
Nozaki 12.881
DTASelect 12.881
Thurlkill 12.881
EMBOSS 13.378
Sillero 12.881
Patrickios 12.106
IPC_peptide 13.378
IPC2_peptide 12.369
IPC2.peptide.svr19 9.178
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3992
0
3992
1214804
28
2140
304.3
33.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.491 ± 0.05
0.682 ± 0.011
5.594 ± 0.031
6.084 ± 0.04
3.878 ± 0.028
8.636 ± 0.035
1.988 ± 0.019
5.414 ± 0.028
2.819 ± 0.029
10.164 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.494 ± 0.017
2.62 ± 0.021
5.194 ± 0.027
3.008 ± 0.021
6.919 ± 0.037
5.098 ± 0.028
5.384 ± 0.034
7.765 ± 0.032
1.393 ± 0.018
2.376 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here