Xylona heveae (strain CBS 132557 / TC161)
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8201 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A164Z8C9|A0A164Z8C9_XYLHT L-allo-threonine aldolase OS=Xylona heveae (strain CBS 132557 / TC161) OX=1328760 GN=L228DRAFT_215671 PE=3 SV=1
CC1 pKa = 7.43 PFWCKK6 pKa = 10.29 VDD8 pKa = 4.16 LSGTLAVFACGAVRR22 pKa = 11.84 AGGFEE27 pKa = 3.92 LAEE30 pKa = 4.07 AAGADD35 pKa = 4.04 DD36 pKa = 3.87 QGKK39 pKa = 7.25 TATPDD44 pKa = 3.07 ATSIPLTAEE53 pKa = 3.42 QDD55 pKa = 2.94 IYY57 pKa = 11.72 AAFLNQHH64 pKa = 6.6 RR65 pKa = 11.84 YY66 pKa = 9.79 DD67 pKa = 4.44 LNPEE71 pKa = 3.75 NDD73 pKa = 3.79 LEE75 pKa = 4.4 GTEE78 pKa = 4.4 TLDD81 pKa = 3.91 EE82 pKa = 4.27 QPLNN86 pKa = 3.79
Molecular weight: 9.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.884
IPC_protein 3.808
Toseland 3.617
ProMoST 4.062
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.528
Solomon 3.77
Lehninger 3.732
Nozaki 3.923
DTASelect 4.113
Thurlkill 3.681
EMBOSS 3.745
Sillero 3.923
Patrickios 1.888
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.816
Protein with the highest isoelectric point:
>tr|A0A165GTR4|A0A165GTR4_XYLHT Kinase-like protein OS=Xylona heveae (strain CBS 132557 / TC161) OX=1328760 GN=L228DRAFT_267977 PE=4 SV=1
MM1 pKa = 7.82 RR2 pKa = 11.84 ISSQSHH8 pKa = 3.49 KK9 pKa = 8.84 TFRR12 pKa = 11.84 TKK14 pKa = 10.46 QKK16 pKa = 9.86 LAKK19 pKa = 9.55 AQKK22 pKa = 8.59 QNRR25 pKa = 11.84 PIPQWIRR32 pKa = 11.84 LRR34 pKa = 11.84 TGNTIGYY41 pKa = 6.85 NAKK44 pKa = 9.45 RR45 pKa = 11.84 RR46 pKa = 11.84 HH47 pKa = 4.21 WRR49 pKa = 11.84 KK50 pKa = 7.51 TRR52 pKa = 11.84 LGLL55 pKa = 4.09
Molecular weight: 6.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.39
IPC2_protein 11.052
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.559
Bjellqvist 12.544
Wikipedia 13.027
Rodwell 12.34
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.544
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.076
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.006
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8201
0
8201
4004401
49
5603
488.3
53.97
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.621 ± 0.022
1.147 ± 0.01
5.55 ± 0.02
6.365 ± 0.028
3.736 ± 0.018
6.832 ± 0.024
2.441 ± 0.012
4.837 ± 0.019
4.86 ± 0.023
8.908 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.003 ± 0.01
3.693 ± 0.012
6.199 ± 0.033
4.089 ± 0.018
6.311 ± 0.022
8.89 ± 0.031
5.728 ± 0.02
5.814 ± 0.018
1.325 ± 0.01
2.65 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here