Prevotella sp. 885
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2874 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A264YAC8|A0A264YAC8_9BACT Pyruvate kinase OS=Prevotella sp. 885 OX=2022527 GN=pyk PE=3 SV=1
MM1 pKa = 7.48 AKK3 pKa = 10.21 VIEE6 pKa = 4.11 GLYY9 pKa = 9.7 YY10 pKa = 10.21 SEE12 pKa = 3.62 SHH14 pKa = 6.61 EE15 pKa = 4.22 YY16 pKa = 10.71 VKK18 pKa = 11.17 VEE20 pKa = 3.6 GDD22 pKa = 3.34 FAYY25 pKa = 10.88 VGITDD30 pKa = 4.07 YY31 pKa = 11.27 AQHH34 pKa = 6.72 ALGNVVYY41 pKa = 11.04 VDD43 pKa = 3.67 MPEE46 pKa = 3.9 VDD48 pKa = 4.74 DD49 pKa = 4.11 EE50 pKa = 4.91 VEE52 pKa = 4.0 AEE54 pKa = 4.0 EE55 pKa = 5.49 DD56 pKa = 3.52 FGAVEE61 pKa = 4.37 SVKK64 pKa = 10.6 AASDD68 pKa = 4.44 LISPVSGTVVEE79 pKa = 4.42 VNEE82 pKa = 4.08 ALEE85 pKa = 4.73 DD86 pKa = 3.78 KK87 pKa = 11.04 PEE89 pKa = 4.36 LLNEE93 pKa = 4.05 DD94 pKa = 5.02 AFANWIMKK102 pKa = 9.93 VEE104 pKa = 4.19 LSDD107 pKa = 3.97 KK108 pKa = 11.04 TEE110 pKa = 4.57 LDD112 pKa = 3.53 NLMDD116 pKa = 4.35 AAAYY120 pKa = 9.63 EE121 pKa = 4.41 EE122 pKa = 4.46 LCKK125 pKa = 10.32 EE126 pKa = 4.14
Molecular weight: 13.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.763
IPC2_protein 3.821
IPC_protein 3.77
Toseland 3.592
ProMoST 3.884
Dawson 3.745
Bjellqvist 3.935
Wikipedia 3.63
Rodwell 3.605
Grimsley 3.503
Solomon 3.732
Lehninger 3.681
Nozaki 3.859
DTASelect 3.999
Thurlkill 3.63
EMBOSS 3.643
Sillero 3.884
Patrickios 1.837
IPC_peptide 3.732
IPC2_peptide 3.872
IPC2.peptide.svr19 3.822
Protein with the highest isoelectric point:
>tr|A0A264Y9E0|A0A264Y9E0_9BACT Transcriptional repressor OS=Prevotella sp. 885 OX=2022527 GN=CHL74_03775 PE=3 SV=1
MM1 pKa = 7.17 YY2 pKa = 10.2 FGNRR6 pKa = 11.84 RR7 pKa = 11.84 PRR9 pKa = 11.84 GFHH12 pKa = 4.56 HH13 pKa = 6.66 TFRR16 pKa = 11.84 FSNEE20 pKa = 3.29 RR21 pKa = 11.84 RR22 pKa = 11.84 DD23 pKa = 3.52 ILDD26 pKa = 3.47 ALKK29 pKa = 10.68 RR30 pKa = 11.84 GVAPEE35 pKa = 3.84 DD36 pKa = 3.57 VARR39 pKa = 11.84 QSLEE43 pKa = 3.65 GSSEE47 pKa = 3.82 GDD49 pKa = 2.73 RR50 pKa = 11.84 KK51 pKa = 10.17 SARR54 pKa = 11.84 QPARR58 pKa = 11.84 ITGWLFIVALLLLLAALMALLACC81 pKa = 5.29
Molecular weight: 9.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.331
IPC2_protein 9.502
IPC_protein 10.657
Toseland 10.818
ProMoST 10.862
Dawson 10.862
Bjellqvist 10.701
Wikipedia 11.184
Rodwell 10.804
Grimsley 10.906
Solomon 11.155
Lehninger 11.096
Nozaki 10.804
DTASelect 10.687
Thurlkill 10.804
EMBOSS 11.257
Sillero 10.833
Patrickios 10.643
IPC_peptide 11.155
IPC2_peptide 10.028
IPC2.peptide.svr19 8.856
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2874
0
2874
1060643
18
4979
369.0
41.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.591 ± 0.037
1.34 ± 0.017
6.171 ± 0.033
6.307 ± 0.046
4.295 ± 0.028
6.758 ± 0.045
1.952 ± 0.021
6.354 ± 0.038
6.66 ± 0.042
8.38 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.958 ± 0.021
5.06 ± 0.041
3.53 ± 0.023
3.425 ± 0.026
4.83 ± 0.044
6.146 ± 0.037
5.993 ± 0.042
6.69 ± 0.034
1.241 ± 0.02
4.318 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here