Taibaiella sp. KVB11
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3591 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5M6CNF1|A0A5M6CNF1_9BACT Guanylate cyclase domain-containing protein OS=Taibaiella sp. KVB11 OX=2608001 GN=F0919_09410 PE=3 SV=1
MM1 pKa = 7.73 RR2 pKa = 11.84 VDD4 pKa = 4.22 EE5 pKa = 5.66 KK6 pKa = 10.72 MLCIYY11 pKa = 10.27 FDD13 pKa = 3.46 SAVFDD18 pKa = 3.55 FVYY21 pKa = 10.3 RR22 pKa = 11.84 VFASVMVVFDD32 pKa = 4.08 FVSIAFVFLSAFADD46 pKa = 3.8 LVHH49 pKa = 6.61 AVIVLLSMVFDD60 pKa = 4.45 FAPEE64 pKa = 3.89 VFDD67 pKa = 4.24 FVSAVFDD74 pKa = 3.79 FVSAVFDD81 pKa = 3.97 FVSAAFDD88 pKa = 3.58 FVSAVFDD95 pKa = 3.68 FMSAVFDD102 pKa = 4.25 FVSEE106 pKa = 4.28 GFDD109 pKa = 3.14 LYY111 pKa = 11.17 YY112 pKa = 10.39 IKK114 pKa = 9.78 TDD116 pKa = 3.28 YY117 pKa = 10.39 MFEE120 pKa = 4.43 GFAQMM125 pKa = 5.09
Molecular weight: 14.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.579
IPC_protein 3.567
Toseland 3.35
ProMoST 3.783
Dawson 3.592
Bjellqvist 3.757
Wikipedia 3.579
Rodwell 3.401
Grimsley 3.261
Solomon 3.567
Lehninger 3.528
Nozaki 3.719
DTASelect 3.999
Thurlkill 3.427
EMBOSS 3.579
Sillero 3.706
Patrickios 0.769
IPC_peptide 3.554
IPC2_peptide 3.668
IPC2.peptide.svr19 3.673
Protein with the highest isoelectric point:
>tr|A0A5M6CNQ9|A0A5M6CNQ9_9BACT Phosphoheptose isomerase OS=Taibaiella sp. KVB11 OX=2608001 GN=gmhA PE=3 SV=1
MM1 pKa = 7.69 PKK3 pKa = 10.34 VKK5 pKa = 8.9 THH7 pKa = 5.39 SRR9 pKa = 11.84 AKK11 pKa = 9.54 KK12 pKa = 7.25 TFKK15 pKa = 10.04 VTGTGKK21 pKa = 8.03 ITRR24 pKa = 11.84 RR25 pKa = 11.84 KK26 pKa = 9.96 AFRR29 pKa = 11.84 KK30 pKa = 9.6 HH31 pKa = 6.25 LLTKK35 pKa = 10.37 KK36 pKa = 8.3 SAKK39 pKa = 9.48 RR40 pKa = 11.84 RR41 pKa = 11.84 RR42 pKa = 11.84 SLDD45 pKa = 2.88 GSVFVHH51 pKa = 6.33 SANLPLVKK59 pKa = 10.37 RR60 pKa = 11.84 MLNMRR65 pKa = 4.51
Molecular weight: 7.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.387
IPC2_protein 10.847
IPC_protein 12.384
Toseland 12.574
ProMoST 13.056
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.042
Rodwell 12.471
Grimsley 12.618
Solomon 13.056
Lehninger 12.954
Nozaki 12.574
DTASelect 12.559
Thurlkill 12.574
EMBOSS 13.056
Sillero 12.574
Patrickios 12.193
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 9.014
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3591
0
3591
1235045
25
3657
343.9
38.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.358 ± 0.044
0.973 ± 0.017
5.342 ± 0.03
5.597 ± 0.057
4.888 ± 0.036
6.803 ± 0.053
1.875 ± 0.02
7.497 ± 0.04
6.791 ± 0.057
8.96 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.516 ± 0.02
6.159 ± 0.046
3.806 ± 0.03
3.726 ± 0.027
3.526 ± 0.03
6.518 ± 0.039
6.208 ± 0.076
6.129 ± 0.032
1.134 ± 0.013
4.194 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here