Triplophysa tibetana

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Otomorpha; Ostariophysi; Otophysi;

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23866 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5A9P593|A0A5A9P593_9TELE Semaphorin-4C M-Sema F OS=Triplophysa tibetana OX=1572043 GN=E1301_Tti009000 PE=3 SV=1
MM1 pKa = 7.02VRR3 pKa = 11.84WSEE6 pKa = 4.64DD7 pKa = 3.02GVNLHH12 pKa = 6.52GKK14 pKa = 9.53NDD16 pKa = 3.83GGSLGDD22 pKa = 4.08GLEE25 pKa = 4.07MGCGEE30 pKa = 4.22FHH32 pKa = 7.71DD33 pKa = 4.56SDD35 pKa = 3.82VMEE38 pKa = 4.75WGNVDD43 pKa = 3.44GNLNEE48 pKa = 4.68GNDD51 pKa = 4.41GVEE54 pKa = 3.72MGYY57 pKa = 10.78DD58 pKa = 3.95EE59 pKa = 5.37IHH61 pKa = 7.45DD62 pKa = 4.45DD63 pKa = 4.79DD64 pKa = 6.09VMEE67 pKa = 4.37EE68 pKa = 4.01WTVDD72 pKa = 3.24GNLNEE77 pKa = 4.72GNDD80 pKa = 4.41GVEE83 pKa = 3.72MGYY86 pKa = 10.78DD87 pKa = 3.95EE88 pKa = 5.37IHH90 pKa = 7.45DD91 pKa = 4.45DD92 pKa = 4.79DD93 pKa = 6.09VMEE96 pKa = 4.37EE97 pKa = 4.01WTVDD101 pKa = 3.24GNLNEE106 pKa = 4.43EE107 pKa = 4.16NDD109 pKa = 4.41GVEE112 pKa = 4.12MGCGVLHH119 pKa = 7.72DD120 pKa = 5.02DD121 pKa = 4.72GVMEE125 pKa = 5.04GGNVDD130 pKa = 3.55GNLNEE135 pKa = 4.68GNDD138 pKa = 4.31GVEE141 pKa = 3.59MGYY144 pKa = 9.55EE145 pKa = 3.95QFHH148 pKa = 7.3DD149 pKa = 3.99DD150 pKa = 4.07DD151 pKa = 3.95VTRR154 pKa = 11.84EE155 pKa = 4.09GNVDD159 pKa = 3.31GNLNEE164 pKa = 4.4EE165 pKa = 4.16NDD167 pKa = 4.39GVEE170 pKa = 3.99MGCDD174 pKa = 3.38KK175 pKa = 11.14FHH177 pKa = 6.9GGYY180 pKa = 11.33VMMVNDD186 pKa = 4.06GDD188 pKa = 4.15VFHH191 pKa = 7.38DD192 pKa = 3.45RR193 pKa = 11.84CVVGEE198 pKa = 3.99ILIGNDD204 pKa = 3.43DD205 pKa = 3.95GHH207 pKa = 7.7FLILKK212 pKa = 10.2NKK214 pKa = 8.96DD215 pKa = 3.18LKK217 pKa = 11.09VDD219 pKa = 3.87YY220 pKa = 10.89EE221 pKa = 4.14NLLCKK226 pKa = 10.36SGLMKK231 pKa = 10.93GFGDD235 pKa = 4.22FPEE238 pKa = 4.56VCPGLDD244 pKa = 3.71GLWTFVTPRR253 pKa = 11.84DD254 pKa = 3.43PDD256 pKa = 3.62VYY258 pKa = 11.12LDD260 pKa = 3.57GVQRR264 pKa = 11.84NDD266 pKa = 2.73GDD268 pKa = 3.86FFGSHH273 pKa = 7.2DD274 pKa = 4.08GYY276 pKa = 11.73NGLEE280 pKa = 4.15CDD282 pKa = 4.38GKK284 pKa = 9.48HH285 pKa = 6.4CCSQMHH291 pKa = 6.19YY292 pKa = 10.95GG293 pKa = 3.62

Molecular weight: 32.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5A9PJ90|A0A5A9PJ90_9TELE Uncharacterized protein OS=Triplophysa tibetana OX=1572043 GN=E1301_Tti011490 PE=4 SV=1
MM1 pKa = 7.22QGTGRR6 pKa = 11.84VVKK9 pKa = 9.72RR10 pKa = 11.84TGRR13 pKa = 11.84VVKK16 pKa = 9.71RR17 pKa = 11.84TGRR20 pKa = 11.84VVKK23 pKa = 9.71RR24 pKa = 11.84TGRR27 pKa = 11.84VVKK30 pKa = 9.71RR31 pKa = 11.84TGRR34 pKa = 11.84VVKK37 pKa = 9.71RR38 pKa = 11.84TGRR41 pKa = 11.84VVKK44 pKa = 9.71RR45 pKa = 11.84TGRR48 pKa = 11.84VVKK51 pKa = 9.71RR52 pKa = 11.84TGRR55 pKa = 11.84VVKK58 pKa = 9.61GTGRR62 pKa = 11.84VVKK65 pKa = 9.73RR66 pKa = 11.84TGRR69 pKa = 11.84VVKK72 pKa = 9.71RR73 pKa = 11.84TGRR76 pKa = 11.84VVKK79 pKa = 9.71RR80 pKa = 11.84TGRR83 pKa = 11.84VVKK86 pKa = 9.71RR87 pKa = 11.84TGRR90 pKa = 11.84VVKK93 pKa = 9.61GTGRR97 pKa = 11.84VVKK100 pKa = 9.73RR101 pKa = 11.84TGRR104 pKa = 11.84VVKK107 pKa = 9.71RR108 pKa = 11.84TGRR111 pKa = 11.84VVKK114 pKa = 9.61GTGRR118 pKa = 11.84VVKK121 pKa = 10.16GRR123 pKa = 11.84TSGRR127 pKa = 11.84VVKK130 pKa = 9.81RR131 pKa = 11.84TGRR134 pKa = 11.84VVKK137 pKa = 9.61GTGRR141 pKa = 11.84VVKK144 pKa = 9.62GTGRR148 pKa = 11.84VVKK151 pKa = 9.82STGRR155 pKa = 11.84VVKK158 pKa = 9.74RR159 pKa = 11.84TGRR162 pKa = 11.84VVKK165 pKa = 9.61GTGRR169 pKa = 11.84VVKK172 pKa = 9.82STGRR176 pKa = 11.84VVKK179 pKa = 9.74RR180 pKa = 11.84TGRR183 pKa = 11.84VVKK186 pKa = 9.71RR187 pKa = 11.84TGRR190 pKa = 11.84VVKK193 pKa = 10.28RR194 pKa = 11.84PQEE197 pKa = 3.89HH198 pKa = 7.03RR199 pKa = 11.84RR200 pKa = 11.84DD201 pKa = 3.42QCKK204 pKa = 10.3

Molecular weight: 22.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23866

0

23866

13339987

50

45354

559.0

62.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.118 ± 0.013

2.306 ± 0.013

5.314 ± 0.012

6.89 ± 0.023

3.682 ± 0.01

6.047 ± 0.02

2.701 ± 0.009

4.662 ± 0.014

5.794 ± 0.021

9.413 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.007

4.085 ± 0.012

5.43 ± 0.02

4.646 ± 0.017

5.63 ± 0.012

8.766 ± 0.024

5.834 ± 0.02

6.395 ± 0.016

1.134 ± 0.006

2.72 ± 0.009
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski