Parapusillimonas sp. SGNA-6

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Parapusillimonas; unclassified Parapusillimonas

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5275 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M1Q929|A0A6M1Q929_9BURK Enoyl-[acyl-carrier-protein] reductase FabV (Fragment) OS=Parapusillimonas sp. SGNA-6 OX=2710884 GN=fabV PE=4 SV=1
MM1 pKa = 8.01AITGATTVDD10 pKa = 3.12NAIILTDD17 pKa = 3.77DD18 pKa = 3.46AAAISNSANVTLSGAIGGTGDD39 pKa = 4.08LYY41 pKa = 11.37KK42 pKa = 10.8EE43 pKa = 4.35GTGEE47 pKa = 4.04LTLSGTNTYY56 pKa = 10.8AGRR59 pKa = 11.84TYY61 pKa = 10.77ISAGTLNITGSILGGANNNAKK82 pKa = 8.96LTGTGVITGDD92 pKa = 3.62VYY94 pKa = 10.9TSSGATLAPGVNGVGQLTINGNLTLDD120 pKa = 3.31IEE122 pKa = 4.78SAFEE126 pKa = 4.04VMVNGITAGTDD137 pKa = 3.39YY138 pKa = 11.38SQLKK142 pKa = 10.52LNGEE146 pKa = 4.01GTLRR150 pKa = 11.84GVLSLSGTYY159 pKa = 9.59IPQEE163 pKa = 3.83GDD165 pKa = 3.28SFIFIDD171 pKa = 5.02NDD173 pKa = 3.47GTDD176 pKa = 2.99AFSYY180 pKa = 9.87FFYY183 pKa = 10.6GHH185 pKa = 7.39AEE187 pKa = 3.7GANYY191 pKa = 9.63VFNGEE196 pKa = 4.09NLKK199 pKa = 10.7VGYY202 pKa = 10.48AGGTGNDD209 pKa = 3.65FVLNSPDD216 pKa = 2.75ITAPTITSVTSSTANGTYY234 pKa = 10.32KK235 pKa = 10.27IGDD238 pKa = 3.94VISVQVNFDD247 pKa = 3.24EE248 pKa = 4.67TVNVSGTPRR257 pKa = 11.84LTLEE261 pKa = 4.1TGTTDD266 pKa = 2.91RR267 pKa = 11.84TVDD270 pKa = 3.68YY271 pKa = 11.29VSGTGTSTLTFTYY284 pKa = 9.38TIQAGDD290 pKa = 3.62EE291 pKa = 4.42SVDD294 pKa = 3.46LDD296 pKa = 4.09YY297 pKa = 11.54AATTALALNSGSIQDD312 pKa = 3.99LAGNNATLTLFAPGAAGSLGANKK335 pKa = 10.22DD336 pKa = 3.32IVVDD340 pKa = 4.46GVRR343 pKa = 11.84PTATLVVTDD352 pKa = 4.43AALSAGEE359 pKa = 4.13TSLVTITFSEE369 pKa = 4.48AVTGFTNADD378 pKa = 3.29LTIANGTLSAISSSDD393 pKa = 3.71GGITWTATFTPTANIEE409 pKa = 4.1DD410 pKa = 3.99ATNVITLDD418 pKa = 3.51NTGVADD424 pKa = 4.09LAGNAGTGTTEE435 pKa = 4.11SNNYY439 pKa = 10.38AIDD442 pKa = 3.74TKK444 pKa = 10.87RR445 pKa = 11.84PTATIVVV452 pKa = 3.97

Molecular weight: 45.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M1PX30|A0A6M1PX30_9BURK Elongation factor G OS=Parapusillimonas sp. SGNA-6 OX=2710884 GN=fusA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5275

0

5275

1582118

23

2843

299.9

32.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.612 ± 0.051

0.878 ± 0.011

5.583 ± 0.025

5.493 ± 0.034

3.819 ± 0.025

7.918 ± 0.037

2.272 ± 0.017

5.563 ± 0.031

4.16 ± 0.038

10.148 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.518 ± 0.016

3.389 ± 0.025

4.81 ± 0.023

3.806 ± 0.023

6.202 ± 0.032

5.843 ± 0.024

5.34 ± 0.024

7.35 ± 0.033

1.355 ± 0.017

2.941 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski