Paenibacillus beijingensis
Average proteome isoelectric point is 6.47
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4723 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D5NEH5|A0A0D5NEH5_9BACL Formamidopyrimidine-DNA glycosylase OS=Paenibacillus beijingensis OX=1126833 GN=mutM PE=3 SV=1
MM1 pKa = 7.64 AKK3 pKa = 10.37 YY4 pKa = 10.41 SWVDD8 pKa = 2.75 KK9 pKa = 9.16 DD10 pKa = 3.48 TCIACGACGATAPDD24 pKa = 3.47 IYY26 pKa = 11.02 DD27 pKa = 3.64 YY28 pKa = 11.51 DD29 pKa = 4.43 DD30 pKa = 3.97 EE31 pKa = 5.76 GLAEE35 pKa = 4.4 VIYY38 pKa = 11.01 DD39 pKa = 3.28 GDD41 pKa = 3.95 GNHH44 pKa = 6.17 GVKK47 pKa = 10.54 AIAEE51 pKa = 4.12 DD52 pKa = 5.08 LYY54 pKa = 11.61 DD55 pKa = 5.12 DD56 pKa = 5.55 LQDD59 pKa = 4.35 ACDD62 pKa = 3.92 GCPTDD67 pKa = 4.24 SIKK70 pKa = 10.77 IADD73 pKa = 3.88 EE74 pKa = 4.08 PFNRR78 pKa = 4.46
Molecular weight: 8.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.808
IPC2_protein 3.643
IPC_protein 3.643
Toseland 3.414
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.656
Rodwell 3.465
Grimsley 3.325
Solomon 3.643
Lehninger 3.605
Nozaki 3.795
DTASelect 4.075
Thurlkill 3.49
EMBOSS 3.656
Sillero 3.77
Patrickios 0.248
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.734
Protein with the highest isoelectric point:
>tr|A0A0D5NI31|A0A0D5NI31_9BACL MarR family transcriptional regulator OS=Paenibacillus beijingensis OX=1126833 GN=VN24_09420 PE=4 SV=1
MM1 pKa = 7.9 RR2 pKa = 11.84 PTFKK6 pKa = 10.82 PNVSKK11 pKa = 10.8 RR12 pKa = 11.84 KK13 pKa = 8.95 KK14 pKa = 8.25 VHH16 pKa = 5.49 GFRR19 pKa = 11.84 KK20 pKa = 9.99 RR21 pKa = 11.84 MSSKK25 pKa = 10.47 NGRR28 pKa = 11.84 KK29 pKa = 9.01 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.23 GRR39 pKa = 11.84 KK40 pKa = 8.56 VLSAA44 pKa = 4.05
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.705
Grimsley 12.91
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.427
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4723
0
4723
1475279
26
3119
312.4
34.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.999 ± 0.048
0.8 ± 0.01
5.038 ± 0.028
6.642 ± 0.043
4.144 ± 0.029
7.859 ± 0.031
2.039 ± 0.018
6.489 ± 0.037
5.121 ± 0.034
10.216 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.805 ± 0.02
3.504 ± 0.026
4.195 ± 0.023
3.611 ± 0.022
5.514 ± 0.04
6.2 ± 0.03
5.169 ± 0.025
7.175 ± 0.029
1.237 ± 0.014
3.243 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here