Clostridiales bacterium CHKCI006

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; unclassified Eubacteriales

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3288 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A143X1A7|A0A143X1A7_9FIRM Uncharacterized protein OS=Clostridiales bacterium CHKCI006 OX=1780379 GN=BN3662_01080 PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 10.51YY3 pKa = 9.31MVMEE7 pKa = 4.27CHH9 pKa = 6.74LSYY12 pKa = 10.87AIVLAEE18 pKa = 4.91DD19 pKa = 3.62GRR21 pKa = 11.84FLKK24 pKa = 10.47VANLHH29 pKa = 5.1YY30 pKa = 10.2QVGEE34 pKa = 3.69IVYY37 pKa = 10.38DD38 pKa = 3.78VVEE41 pKa = 4.47MNMPTPKK48 pKa = 10.03HH49 pKa = 5.96HH50 pKa = 6.78MPHH53 pKa = 5.71IPKK56 pKa = 9.53WGYY59 pKa = 10.48ALSALACCLVLALTSLLWPTSSPYY83 pKa = 11.02ASVYY87 pKa = 10.73LSINPEE93 pKa = 3.42VRR95 pKa = 11.84IDD97 pKa = 3.83VDD99 pKa = 3.62SQDD102 pKa = 3.23QVVDD106 pKa = 3.87LEE108 pKa = 4.57GVNEE112 pKa = 4.39DD113 pKa = 4.93GIALLEE119 pKa = 4.37NYY121 pKa = 8.48TYY123 pKa = 10.71QDD125 pKa = 3.36KK126 pKa = 11.24NLDD129 pKa = 3.61LVMDD133 pKa = 4.51EE134 pKa = 4.62LVDD137 pKa = 3.67RR138 pKa = 11.84AIEE141 pKa = 3.96MGYY144 pKa = 8.3LTEE147 pKa = 5.21GGTVSLVLDD156 pKa = 4.37ADD158 pKa = 4.16DD159 pKa = 4.4NQWVVDD165 pKa = 3.97HH166 pKa = 5.96QTTLSDD172 pKa = 3.67HH173 pKa = 6.33LHH175 pKa = 5.95AHH177 pKa = 7.32LDD179 pKa = 3.54QKK181 pKa = 10.81ISVVIEE187 pKa = 3.72IKK189 pKa = 10.03SARR192 pKa = 11.84DD193 pKa = 3.02ASQQVTIPVEE203 pKa = 4.05PVEE206 pKa = 4.53TPVEE210 pKa = 4.02PEE212 pKa = 3.95PPVVSEE218 pKa = 4.16PVDD221 pKa = 3.59EE222 pKa = 4.72EE223 pKa = 4.5VVEE226 pKa = 4.56TPRR229 pKa = 11.84VEE231 pKa = 5.78DD232 pKa = 4.06GDD234 pKa = 4.3SQYY237 pKa = 11.61SPSDD241 pKa = 3.96DD242 pKa = 5.37DD243 pKa = 6.48DD244 pKa = 6.54DD245 pKa = 7.14DD246 pKa = 5.93SFDD249 pKa = 5.73DD250 pKa = 4.89GDD252 pKa = 4.53SNYY255 pKa = 11.07DD256 pKa = 4.46DD257 pKa = 5.91ADD259 pKa = 3.87EE260 pKa = 6.42DD261 pKa = 5.4DD262 pKa = 6.08DD263 pKa = 7.17DD264 pKa = 5.15DD265 pKa = 4.37

Molecular weight: 29.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A143WWU4|A0A143WWU4_9FIRM HTH-type transcriptional regulator RpiR OS=Clostridiales bacterium CHKCI006 OX=1780379 GN=rpiR_1 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.3QPNKK9 pKa = 9.31RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84QRR14 pKa = 11.84KK15 pKa = 7.94LGFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84RR29 pKa = 11.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3288

0

3288

962042

29

3056

292.6

33.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.024 ± 0.04

1.432 ± 0.017

5.862 ± 0.035

6.898 ± 0.043

4.215 ± 0.029

6.218 ± 0.044

2.464 ± 0.026

7.275 ± 0.043

6.052 ± 0.047

10.197 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.123 ± 0.021

4.056 ± 0.033

3.348 ± 0.023

4.277 ± 0.032

4.495 ± 0.034

5.857 ± 0.036

5.234 ± 0.037

6.443 ± 0.037

0.926 ± 0.018

4.604 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski