Amino acid dipepetide frequency for Clostridiales bacterium CHKCI006

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.675AlaAla: 4.675 ± 0.086	1.314AlaCys: 1.314 ± 0.035	3.778AlaAsp: 3.778 ± 0.072	3.613AlaGlu: 3.613 ± 0.075	3.194AlaPhe: 3.194 ± 0.064	4.552AlaGly: 4.552 ± 0.082	1.585AlaHis: 1.585 ± 0.048	5.428AlaIle: 5.428 ± 0.103	4.839AlaLys: 4.839 ± 0.086	7.937AlaLeu: 7.937 ± 0.103	2.375AlaMet: 2.375 ± 0.053	2.856AlaAsn: 2.856 ± 0.059	1.867AlaPro: 1.867 ± 0.045	2.426AlaGln: 2.426 ± 0.055	3.2AlaArg: 3.2 ± 0.059	4.788AlaSer: 4.788 ± 0.075	3.295AlaThr: 3.295 ± 0.061	4.362AlaVal: 4.362 ± 0.081	0.653AlaTrp: 0.653 ± 0.029	3.501AlaTyr: 3.501 ± 0.078	0.0AlaXaa: 0.0 ± 0.0
Cys	0.837CysAla: 0.837 ± 0.026	0.252CysCys: 0.252 ± 0.019	0.74CysAsp: 0.74 ± 0.03	0.854CysGlu: 0.854 ± 0.032	0.73CysPhe: 0.73 ± 0.031	1.153CysGly: 1.153 ± 0.033	0.496CysHis: 0.496 ± 0.025	1.037CysIle: 1.037 ± 0.032	0.59CysLys: 0.59 ± 0.028	1.731CysLeu: 1.731 ± 0.048	0.414CysMet: 0.414 ± 0.021	0.463CysAsn: 0.463 ± 0.024	0.658CysPro: 0.658 ± 0.033	0.712CysGln: 0.712 ± 0.028	0.649CysArg: 0.649 ± 0.027	0.742CysSer: 0.742 ± 0.027	0.642CysThr: 0.642 ± 0.028	0.896CysVal: 0.896 ± 0.033	0.126CysTrp: 0.126 ± 0.01	0.601CysTyr: 0.601 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	3.947AspAla: 3.947 ± 0.069	0.785AspCys: 0.785 ± 0.027	3.366AspAsp: 3.366 ± 0.076	5.0AspGlu: 5.0 ± 0.092	2.777AspPhe: 2.777 ± 0.063	3.984AspGly: 3.984 ± 0.085	1.777AspHis: 1.777 ± 0.043	4.084AspIle: 4.084 ± 0.064	2.739AspLys: 2.739 ± 0.062	5.97AspLeu: 5.97 ± 0.088	1.743AspMet: 1.743 ± 0.042	1.849AspAsn: 1.849 ± 0.05	2.488AspPro: 2.488 ± 0.052	2.464AspGln: 2.464 ± 0.052	2.634AspArg: 2.634 ± 0.055	2.605AspSer: 2.605 ± 0.055	2.828AspThr: 2.828 ± 0.061	3.762AspVal: 3.762 ± 0.067	0.673AspTrp: 0.673 ± 0.027	3.147AspTyr: 3.147 ± 0.065	0.0AspXaa: 0.0 ± 0.0
Glu	5.628GluAla: 5.628 ± 0.093	0.7GluCys: 0.7 ± 0.03	4.205GluAsp: 4.205 ± 0.066	5.427GluGlu: 5.427 ± 0.084	1.926GluPhe: 1.926 ± 0.045	3.896GluGly: 3.896 ± 0.07	1.442GluHis: 1.442 ± 0.04	5.309GluIle: 5.309 ± 0.072	5.615GluLys: 5.615 ± 0.081	6.344GluLeu: 6.344 ± 0.081	2.644GluMet: 2.644 ± 0.054	3.607GluAsn: 3.607 ± 0.064	1.879GluPro: 1.879 ± 0.045	2.777GluGln: 2.777 ± 0.067	3.331GluArg: 3.331 ± 0.068	3.053GluSer: 3.053 ± 0.06	3.52GluThr: 3.52 ± 0.068	4.614GluVal: 4.614 ± 0.077	0.635GluTrp: 0.635 ± 0.03	2.427GluTyr: 2.427 ± 0.058	0.0GluXaa: 0.0 ± 0.0
Phe	2.788PheAla: 2.788 ± 0.057	0.687PheCys: 0.687 ± 0.029	2.9PheAsp: 2.9 ± 0.055	2.801PheGlu: 2.801 ± 0.056	1.998PhePhe: 1.998 ± 0.064	2.762PheGly: 2.762 ± 0.06	0.956PheHis: 0.956 ± 0.033	2.917PheIle: 2.917 ± 0.06	2.28PheLys: 2.28 ± 0.051	4.164PheLeu: 4.164 ± 0.09	1.147PheMet: 1.147 ± 0.036	1.744PheAsn: 1.744 ± 0.046	1.321PhePro: 1.321 ± 0.036	1.65PheGln: 1.65 ± 0.042	1.524PheArg: 1.524 ± 0.037	2.968PheSer: 2.968 ± 0.056	2.184PheThr: 2.184 ± 0.049	2.924PheVal: 2.924 ± 0.064	0.367PheTrp: 0.367 ± 0.019	2.071PheTyr: 2.071 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	3.845GlyAla: 3.845 ± 0.069	1.05GlyCys: 1.05 ± 0.034	2.977GlyAsp: 2.977 ± 0.067	3.621GlyGlu: 3.621 ± 0.067	2.861GlyPhe: 2.861 ± 0.055	3.881GlyGly: 3.881 ± 0.079	1.577GlyHis: 1.577 ± 0.041	5.31GlyIle: 5.31 ± 0.076	4.359GlyLys: 4.359 ± 0.073	6.12GlyLeu: 6.12 ± 0.102	2.254GlyMet: 2.254 ± 0.053	2.565GlyAsn: 2.565 ± 0.056	1.291GlyPro: 1.291 ± 0.036	2.511GlyGln: 2.511 ± 0.065	2.751GlyArg: 2.751 ± 0.063	3.534GlySer: 3.534 ± 0.069	3.54GlyThr: 3.54 ± 0.065	4.081GlyVal: 4.081 ± 0.076	0.72GlyTrp: 0.72 ± 0.027	3.325GlyTyr: 3.325 ± 0.063	0.0GlyXaa: 0.0 ± 0.0
His	1.441HisAla: 1.441 ± 0.047	0.434HisCys: 0.434 ± 0.022	1.466HisAsp: 1.466 ± 0.036	1.553HisGlu: 1.553 ± 0.039	1.369HisPhe: 1.369 ± 0.039	1.395HisGly: 1.395 ± 0.039	0.93HisHis: 0.93 ± 0.052	1.744HisIle: 1.744 ± 0.047	0.891HisLys: 0.891 ± 0.026	2.895HisLeu: 2.895 ± 0.055	0.655HisMet: 0.655 ± 0.024	0.682HisAsn: 0.682 ± 0.028	1.242HisPro: 1.242 ± 0.038	1.323HisGln: 1.323 ± 0.038	1.156HisArg: 1.156 ± 0.037	1.21HisSer: 1.21 ± 0.033	1.108HisThr: 1.108 ± 0.033	1.472HisVal: 1.472 ± 0.039	0.263HisTrp: 0.263 ± 0.016	1.416HisTyr: 1.416 ± 0.039	0.0HisXaa: 0.0 ± 0.0
Ile	5.496IleAla: 5.496 ± 0.065	1.141IleCys: 1.141 ± 0.037	4.731IleAsp: 4.731 ± 0.07	5.123IleGlu: 5.123 ± 0.081	2.84IlePhe: 2.84 ± 0.064	4.631IleGly: 4.631 ± 0.077	1.727IleHis: 1.727 ± 0.042	4.884IleIle: 4.884 ± 0.088	3.613IleLys: 3.613 ± 0.069	7.201IleLeu: 7.201 ± 0.099	1.968IleMet: 1.968 ± 0.048	2.971IleAsn: 2.971 ± 0.063	2.844IlePro: 2.844 ± 0.057	3.199IleGln: 3.199 ± 0.063	3.31IleArg: 3.31 ± 0.06	4.886IleSer: 4.886 ± 0.084	3.632IleThr: 3.632 ± 0.073	4.897IleVal: 4.897 ± 0.082	0.583IleTrp: 0.583 ± 0.024	3.077IleTyr: 3.077 ± 0.064	0.0IleXaa: 0.0 ± 0.0
Lys	4.661LysAla: 4.661 ± 0.073	0.584LysCys: 0.584 ± 0.028	3.608LysAsp: 3.608 ± 0.06	5.498LysGlu: 5.498 ± 0.085	1.268LysPhe: 1.268 ± 0.04	3.544LysGly: 3.544 ± 0.066	1.386LysHis: 1.386 ± 0.041	4.189LysIle: 4.189 ± 0.074	5.217LysLys: 5.217 ± 0.086	4.706LysLeu: 4.706 ± 0.072	2.156LysMet: 2.156 ± 0.051	2.737LysAsn: 2.737 ± 0.059	1.891LysPro: 1.891 ± 0.048	2.938LysGln: 2.938 ± 0.057	3.116LysArg: 3.116 ± 0.058	2.775LysSer: 2.775 ± 0.059	3.594LysThr: 3.594 ± 0.062	3.669LysVal: 3.669 ± 0.064	0.549LysTrp: 0.549 ± 0.023	2.433LysTyr: 2.433 ± 0.048	0.0LysXaa: 0.0 ± 0.0
Leu	7.982LeuAla: 7.982 ± 0.109	1.63LeuCys: 1.63 ± 0.045	6.347LeuAsp: 6.347 ± 0.075	6.854LeuGlu: 6.854 ± 0.101	4.546LeuPhe: 4.546 ± 0.088	6.096LeuGly: 6.096 ± 0.09	2.06LeuHis: 2.06 ± 0.046	6.796LeuIle: 6.796 ± 0.113	6.746LeuLys: 6.746 ± 0.089	9.85LeuLeu: 9.85 ± 0.143	3.041LeuMet: 3.041 ± 0.05	4.411LeuAsn: 4.411 ± 0.064	3.723LeuPro: 3.723 ± 0.067	3.441LeuGln: 3.441 ± 0.068	3.924LeuArg: 3.924 ± 0.063	7.334LeuSer: 7.334 ± 0.108	5.544LeuThr: 5.544 ± 0.078	6.627LeuVal: 6.627 ± 0.088	0.845LeuTrp: 0.845 ± 0.034	4.17LeuTyr: 4.17 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.375MetAla: 2.375 ± 0.051	0.311MetCys: 0.311 ± 0.019	1.876MetAsp: 1.876 ± 0.05	2.054MetGlu: 2.054 ± 0.049	0.977MetPhe: 0.977 ± 0.031	1.731MetGly: 1.731 ± 0.043	0.703MetHis: 0.703 ± 0.028	2.523MetIle: 2.523 ± 0.051	2.307MetLys: 2.307 ± 0.047	2.914MetLeu: 2.914 ± 0.052	1.092MetMet: 1.092 ± 0.04	1.611MetAsn: 1.611 ± 0.038	1.171MetPro: 1.171 ± 0.032	1.408MetGln: 1.408 ± 0.035	1.42MetArg: 1.42 ± 0.043	1.851MetSer: 1.851 ± 0.049	1.886MetThr: 1.886 ± 0.043	1.887MetVal: 1.887 ± 0.041	0.185MetTrp: 0.185 ± 0.012	0.946MetTyr: 0.946 ± 0.033	0.0MetXaa: 0.0 ± 0.0
Asn	2.968AsnAla: 2.968 ± 0.064	0.563AsnCys: 0.563 ± 0.024	2.406AsnAsp: 2.406 ± 0.053	2.991AsnGlu: 2.991 ± 0.067	1.42AsnPhe: 1.42 ± 0.043	3.157AsnGly: 3.157 ± 0.057	1.132AsnHis: 1.132 ± 0.034	2.82AsnIle: 2.82 ± 0.058	1.977AsnLys: 1.977 ± 0.048	3.724AsnLeu: 3.724 ± 0.063	1.215AsnMet: 1.215 ± 0.039	1.528AsnAsn: 1.528 ± 0.045	1.719AsnPro: 1.719 ± 0.039	2.198AsnGln: 2.198 ± 0.047	1.903AsnArg: 1.903 ± 0.045	1.904AsnSer: 1.904 ± 0.051	2.095AsnThr: 2.095 ± 0.05	2.511AsnVal: 2.511 ± 0.046	0.427AsnTrp: 0.427 ± 0.024	1.902AsnTyr: 1.902 ± 0.049	0.0AsnXaa: 0.0 ± 0.0
Pro	2.179ProAla: 2.179 ± 0.048	0.432ProCys: 0.432 ± 0.022	2.223ProAsp: 2.223 ± 0.049	2.982ProGlu: 2.982 ± 0.063	1.635ProPhe: 1.635 ± 0.04	1.85ProGly: 1.85 ± 0.048	0.773ProHis: 0.773 ± 0.03	2.347ProIle: 2.347 ± 0.054	1.785ProLys: 1.785 ± 0.048	3.477ProLeu: 3.477 ± 0.059	0.868ProMet: 0.868 ± 0.035	1.307ProAsn: 1.307 ± 0.039	0.677ProPro: 0.677 ± 0.027	1.284ProGln: 1.284 ± 0.034	1.15ProArg: 1.15 ± 0.038	2.028ProSer: 2.028 ± 0.046	1.793ProThr: 1.793 ± 0.049	2.568ProVal: 2.568 ± 0.051	0.316ProTrp: 0.316 ± 0.017	1.802ProTyr: 1.802 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	3.597GlnAla: 3.597 ± 0.063	0.521GlnCys: 0.521 ± 0.024	2.427GlnAsp: 2.427 ± 0.053	3.089GlnGlu: 3.089 ± 0.064	1.626GlnPhe: 1.626 ± 0.044	2.376GlnGly: 2.376 ± 0.057	0.828GlnHis: 0.828 ± 0.033	3.018GlnIle: 3.018 ± 0.059	2.98GlnLys: 2.98 ± 0.047	4.397GlnLeu: 4.397 ± 0.077	1.429GlnMet: 1.429 ± 0.041	1.817GlnAsn: 1.817 ± 0.05	1.379GlnPro: 1.379 ± 0.043	1.754GlnGln: 1.754 ± 0.056	1.969GlnArg: 1.969 ± 0.058	2.33GlnSer: 2.33 ± 0.059	2.342GlnThr: 2.342 ± 0.061	2.563GlnVal: 2.563 ± 0.057	0.475GlnTrp: 0.475 ± 0.022	1.858GlnTyr: 1.858 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	2.554ArgAla: 2.554 ± 0.06	0.586ArgCys: 0.586 ± 0.024	2.224ArgAsp: 2.224 ± 0.053	3.26ArgGlu: 3.26 ± 0.063	2.154ArgPhe: 2.154 ± 0.052	2.082ArgGly: 2.082 ± 0.049	1.223ArgHis: 1.223 ± 0.037	3.511ArgIle: 3.511 ± 0.073	2.898ArgLys: 2.898 ± 0.07	5.123ArgLeu: 5.123 ± 0.083	1.512ArgMet: 1.512 ± 0.041	1.707ArgAsn: 1.707 ± 0.039	1.378ArgPro: 1.378 ± 0.041	2.537ArgGln: 2.537 ± 0.053	2.284ArgArg: 2.284 ± 0.05	2.371ArgSer: 2.371 ± 0.05	2.071ArgThr: 2.071 ± 0.039	2.544ArgVal: 2.544 ± 0.051	0.411ArgTrp: 0.411 ± 0.021	2.519ArgTyr: 2.519 ± 0.052	0.0ArgXaa: 0.0 ± 0.0
Ser	3.558SerAla: 3.558 ± 0.073	0.836SerCys: 0.836 ± 0.027	3.12SerAsp: 3.12 ± 0.058	3.654SerGlu: 3.654 ± 0.057	2.906SerPhe: 2.906 ± 0.06	4.158SerGly: 4.158 ± 0.074	1.656SerHis: 1.656 ± 0.044	4.013SerIle: 4.013 ± 0.068	3.081SerLys: 3.081 ± 0.061	6.515SerLeu: 6.515 ± 0.112	1.818SerMet: 1.818 ± 0.037	2.146SerAsn: 2.146 ± 0.055	1.71SerPro: 1.71 ± 0.044	2.855SerGln: 2.855 ± 0.062	2.976SerArg: 2.976 ± 0.05	3.779SerSer: 3.779 ± 0.081	2.785SerThr: 2.785 ± 0.055	3.644SerVal: 3.644 ± 0.065	0.617SerTrp: 0.617 ± 0.026	2.739SerTyr: 2.739 ± 0.051	0.0SerXaa: 0.0 ± 0.0
Thr	3.366ThrAla: 3.366 ± 0.069	0.688ThrCys: 0.688 ± 0.027	2.857ThrAsp: 2.857 ± 0.057	2.872ThrGlu: 2.872 ± 0.058	2.464ThrPhe: 2.464 ± 0.051	3.639ThrGly: 3.639 ± 0.064	1.315ThrHis: 1.315 ± 0.045	4.247ThrIle: 4.247 ± 0.078	2.679ThrLys: 2.679 ± 0.053	5.759ThrLeu: 5.759 ± 0.08	1.495ThrMet: 1.495 ± 0.045	1.974ThrAsn: 1.974 ± 0.047	2.12ThrPro: 2.12 ± 0.05	1.867ThrGln: 1.867 ± 0.043	2.179ThrArg: 2.179 ± 0.048	3.292ThrSer: 3.292 ± 0.066	2.949ThrThr: 2.949 ± 0.074	3.599ThrVal: 3.599 ± 0.083	0.406ThrTrp: 0.406 ± 0.018	2.578ThrTyr: 2.578 ± 0.055	0.0ThrXaa: 0.0 ± 0.0
Val	4.49ValAla: 4.49 ± 0.078	0.974ValCys: 0.974 ± 0.033	3.931ValAsp: 3.931 ± 0.072	4.15ValGlu: 4.15 ± 0.077	2.844ValPhe: 2.844 ± 0.061	3.87ValGly: 3.87 ± 0.072	1.406ValHis: 1.406 ± 0.04	4.962ValIle: 4.962 ± 0.082	3.679ValLys: 3.679 ± 0.068	6.698ValLeu: 6.698 ± 0.109	1.944ValMet: 1.944 ± 0.045	2.662ValAsn: 2.662 ± 0.053	2.114ValPro: 2.114 ± 0.05	2.31ValGln: 2.31 ± 0.048	2.662ValArg: 2.662 ± 0.052	4.408ValSer: 4.408 ± 0.084	3.585ValThr: 3.585 ± 0.079	4.415ValVal: 4.415 ± 0.085	0.503ValTrp: 0.503 ± 0.024	2.819ValTyr: 2.819 ± 0.054	0.0ValXaa: 0.0 ± 0.0
Trp	0.48TrpAla: 0.48 ± 0.023	0.147TrpCys: 0.147 ± 0.013	0.51TrpAsp: 0.51 ± 0.023	0.511TrpGlu: 0.511 ± 0.025	0.425TrpPhe: 0.425 ± 0.024	0.533TrpGly: 0.533 ± 0.024	0.246TrpHis: 0.246 ± 0.016	0.827TrpIle: 0.827 ± 0.032	0.525TrpLys: 0.525 ± 0.023	1.16TrpLeu: 1.16 ± 0.031	0.328TrpMet: 0.328 ± 0.019	0.415TrpAsn: 0.415 ± 0.021	0.216TrpPro: 0.216 ± 0.016	0.579TrpGln: 0.579 ± 0.028	0.388TrpArg: 0.388 ± 0.019	0.457TrpSer: 0.457 ± 0.024	0.459TrpThr: 0.459 ± 0.024	0.523TrpVal: 0.523 ± 0.021	0.09TrpTrp: 0.09 ± 0.009	0.442TrpTyr: 0.442 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.374TyrAla: 3.374 ± 0.06	0.687TyrCys: 0.687 ± 0.026	2.928TyrAsp: 2.928 ± 0.054	3.071TyrGlu: 3.071 ± 0.063	2.192TyrPhe: 2.192 ± 0.052	2.886TyrGly: 2.886 ± 0.061	1.425TyrHis: 1.425 ± 0.042	2.787TyrIle: 2.787 ± 0.062	1.719TyrLys: 1.719 ± 0.048	5.285TyrLeu: 5.285 ± 0.089	1.128TyrMet: 1.128 ± 0.033	1.509TyrAsn: 1.509 ± 0.045	1.786TyrPro: 1.786 ± 0.044	2.541TyrGln: 2.541 ± 0.054	2.424TyrArg: 2.424 ± 0.052	2.25TyrSer: 2.25 ± 0.049	2.492TyrThr: 2.492 ± 0.06	2.87TyrVal: 2.87 ± 0.047	0.419TyrTrp: 0.419 ± 0.022	2.263TyrTyr: 2.263 ± 0.062	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3288 proteins (962042 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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