Rhizophagus sp. MUCL 43196
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 22985 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A372QEK2|A0A372QEK2_9GLOM Uncharacterized protein OS=Rhizophagus sp. MUCL 43196 OX=1803374 GN=C1646_770661 PE=4 SV=1
MM1 pKa = 7.81 DD2 pKa = 4.53 NVHH5 pKa = 6.29 KK6 pKa = 10.69 RR7 pKa = 11.84 HH8 pKa = 5.82 LFEE11 pKa = 5.03 MYY13 pKa = 10.3 SSADD17 pKa = 3.67 EE18 pKa = 4.9 EE19 pKa = 4.19 IDD21 pKa = 5.83 DD22 pKa = 4.44 INEE25 pKa = 3.98 LQDD28 pKa = 3.33 EE29 pKa = 4.65 YY30 pKa = 11.54 KK31 pKa = 10.57 SVSSEE36 pKa = 3.88 EE37 pKa = 4.15 DD38 pKa = 3.24 CVLFDD43 pKa = 4.58 LEE45 pKa = 4.81 EE46 pKa = 4.49 NFVSNRR52 pKa = 11.84 QLSPEE57 pKa = 3.79 ILEE60 pKa = 4.45 KK61 pKa = 10.64 IKK63 pKa = 10.69 FLVNIDD69 pKa = 4.07 CGAGPIIRR77 pKa = 11.84 ALQNIFSEE85 pKa = 4.84 TVIHH89 pKa = 6.7 PKK91 pKa = 10.18 VEE93 pKa = 3.96 DD94 pKa = 3.56 WNDD97 pKa = 3.25 LLKK100 pKa = 11.23 NEE102 pKa = 4.13 ITVGEE107 pKa = 4.27 GQDD110 pKa = 3.55 EE111 pKa = 4.33 DD112 pKa = 5.82 GEE114 pKa = 4.56 DD115 pKa = 3.5 NEE117 pKa = 5.37 DD118 pKa = 3.88 YY119 pKa = 11.23 EE120 pKa = 5.54 DD121 pKa = 5.27 GEE123 pKa = 4.74 DD124 pKa = 5.15 GEE126 pKa = 4.95 DD127 pKa = 3.76 GNYY130 pKa = 10.77 GDD132 pKa = 6.21 DD133 pKa = 4.76 DD134 pKa = 6.16 DD135 pKa = 6.28 EE136 pKa = 7.01 DD137 pKa = 3.92 EE138 pKa = 4.78 QSDD141 pKa = 5.19 DD142 pKa = 4.31 EE143 pKa = 4.45 EE144 pKa = 4.98 QDD146 pKa = 4.26 DD147 pKa = 4.0 VDD149 pKa = 3.96 TRR151 pKa = 11.84 SEE153 pKa = 3.9 KK154 pKa = 11.24 LNFFEE159 pKa = 6.52 DD160 pKa = 4.6 DD161 pKa = 3.95 YY162 pKa = 11.66 EE163 pKa = 4.97 SSIVNLHH170 pKa = 5.52 TLITYY175 pKa = 9.59 HH176 pKa = 5.43 NHH178 pKa = 5.47 ANIHH182 pKa = 5.51 EE183 pKa = 4.78 FICDD187 pKa = 3.52 SGCMKK192 pKa = 10.49 FCNNPFLFSGFGSTFQNEE210 pKa = 4.19 ANNFHH215 pKa = 6.93 INLLSIILHH224 pKa = 6.71 MITISILTCYY234 pKa = 9.95 FAISVYY240 pKa = 10.39 DD241 pKa = 3.96 ASDD244 pKa = 3.27 EE245 pKa = 4.13 LLL247 pKa = 4.28
Molecular weight: 28.37 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.7
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.605
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.101
Thurlkill 3.643
EMBOSS 3.706
Sillero 3.923
Patrickios 1.303
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A372QQP0|A0A372QQP0_9GLOM Uncharacterized protein OS=Rhizophagus sp. MUCL 43196 OX=1803374 GN=C1646_712387 PE=4 SV=1
RR1 pKa = 8.37 SPFSIVFPFPFPLRR15 pKa = 11.84 FLSPSFSLFPFPIAFPFPFPFHH37 pKa = 7.14 SPLSLPFVFTFPFVPFSFVFPFPFRR62 pKa = 11.84 SSLSISLRR70 pKa = 11.84 FPFVFPFPFVFPFPNRR86 pKa = 11.84 LPLFPSSSPFPSSSPFPFVSHH107 pKa = 6.78 FPNRR111 pKa = 11.84 LPFPLRR117 pKa = 11.84 FRR119 pKa = 11.84 FFQLSSPFPFRR130 pKa = 11.84 FPFPLRR136 pKa = 11.84 FPFPFIFPFPFVFPFPFVFPFPFVFPFPFVFPFPFVFPP174 pKa = 4.67
Molecular weight: 20.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.513
IPC2_protein 11.184
IPC_protein 12.793
Toseland 12.954
ProMoST 13.466
Dawson 12.954
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.457
Grimsley 12.998
Solomon 13.451
Lehninger 13.349
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.451
Sillero 12.954
Patrickios 12.193
IPC_peptide 13.451
IPC2_peptide 12.442
IPC2.peptide.svr19 9.204
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
22984
1
22985
7538093
49
4766
328.0
37.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.406 ± 0.015
1.558 ± 0.008
5.8 ± 0.012
7.011 ± 0.017
4.692 ± 0.012
4.483 ± 0.015
2.146 ± 0.007
8.324 ± 0.016
7.96 ± 0.019
9.132 ± 0.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.988 ± 0.006
7.163 ± 0.018
4.018 ± 0.015
3.72 ± 0.014
4.279 ± 0.013
7.851 ± 0.018
5.229 ± 0.012
4.974 ± 0.012
1.175 ± 0.006
4.067 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here