Polytolypa hystricis UAMH7299

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Onygenales; Ajellomycetaceae; Polytolypa; Polytolypa hystricis

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9934 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2B7X197|A0A2B7X197_9EURO Uncharacterized protein OS=Polytolypa hystricis UAMH7299 OX=1447883 GN=AJ80_08352 PE=4 SV=1
MM1 pKa = 7.74ASFEE5 pKa = 4.24YY6 pKa = 10.7TPDD9 pKa = 3.47SPQLFTLEE17 pKa = 5.03DD18 pKa = 3.36IDD20 pKa = 5.52RR21 pKa = 11.84NVPVNSIINHH31 pKa = 5.48TPSSLPALADD41 pKa = 3.84FPLDD45 pKa = 3.73FTLEE49 pKa = 4.17TCTLEE54 pKa = 4.08AHH56 pKa = 6.66PGEE59 pKa = 4.87HH60 pKa = 7.01PLDD63 pKa = 3.42IFQIAVSPVPSDD75 pKa = 3.36PTRR78 pKa = 11.84EE79 pKa = 3.75AMYY82 pKa = 8.82ATFADD87 pKa = 4.99CSAPPSTVSNINNIDD102 pKa = 3.46WEE104 pKa = 4.29EE105 pKa = 4.4LISFDD110 pKa = 4.68SPAPEE115 pKa = 4.01EE116 pKa = 4.1TLSCLNMPDD125 pKa = 3.83LSNNSMEE132 pKa = 4.49HH133 pKa = 6.82PSTEE137 pKa = 3.47IHH139 pKa = 6.17QPPLDD144 pKa = 3.94IPHH147 pKa = 7.27LSGDD151 pKa = 4.13SIEE154 pKa = 4.95LPLAGMQPPPQEE166 pKa = 3.91MTDD169 pKa = 3.74FSGASSKK176 pKa = 10.64KK177 pKa = 7.72PCSVEE182 pKa = 3.87PCTSEE187 pKa = 4.25ADD189 pKa = 3.81NII191 pKa = 4.08

Molecular weight: 20.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2B7XAX9|A0A2B7XAX9_9EURO Uncharacterized protein OS=Polytolypa hystricis UAMH7299 OX=1447883 GN=AJ80_07761 PE=4 SV=1
MM1 pKa = 7.23FFSHH5 pKa = 6.45EE6 pKa = 3.78LSLRR10 pKa = 11.84IHH12 pKa = 6.14CRR14 pKa = 11.84ARR16 pKa = 11.84ITNSKK21 pKa = 9.28QAGFAKK27 pKa = 10.06CVPKK31 pKa = 10.8LSLATKK37 pKa = 7.15THH39 pKa = 5.76SRR41 pKa = 11.84SQIARR46 pKa = 11.84RR47 pKa = 11.84TATVIAASACFCGYY61 pKa = 10.48YY62 pKa = 10.13SSCNRR67 pKa = 11.84WWSRR71 pKa = 11.84RR72 pKa = 11.84PRR74 pKa = 11.84SYY76 pKa = 11.44NSFAPRR82 pKa = 11.84VRR84 pKa = 11.84PPALEE89 pKa = 4.18LPVMPEE95 pKa = 4.12KK96 pKa = 10.6IHH98 pKa = 6.01VPKK101 pKa = 10.23LPEE104 pKa = 3.91MPEE107 pKa = 4.38PISALEE113 pKa = 4.21LPTMLEE119 pKa = 4.39EE120 pKa = 4.59IPVPEE125 pKa = 4.48PSRR128 pKa = 11.84PLLAAIGAFWALKK141 pKa = 10.13SSALRR146 pKa = 11.84RR147 pKa = 11.84LNVAPKK153 pKa = 10.34

Molecular weight: 17.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9934

0

9934

4766470

31

7287

479.8

53.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.312 ± 0.023

1.199 ± 0.009

5.72 ± 0.015

6.31 ± 0.027

3.656 ± 0.017

6.77 ± 0.022

2.415 ± 0.009

4.961 ± 0.017

4.876 ± 0.021

8.913 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.081 ± 0.009

3.727 ± 0.011

6.252 ± 0.03

3.998 ± 0.016

6.26 ± 0.02

8.568 ± 0.03

5.937 ± 0.016

5.97 ± 0.017

1.366 ± 0.009

2.708 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski