Phormidesmis priestleyi ULC007
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5141 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2T1D6C9|A0A2T1D6C9_9CYAN SRPBCC family protein OS=Phormidesmis priestleyi ULC007 OX=1920490 GN=C7B65_22850 PE=4 SV=1
MM1 pKa = 7.46 LKK3 pKa = 10.39 DD4 pKa = 4.18 IISAHH9 pKa = 6.95 PISPYY14 pKa = 9.75 QIHH17 pKa = 6.82 LRR19 pKa = 11.84 FEE21 pKa = 4.73 DD22 pKa = 4.95 GIEE25 pKa = 4.05 GTIDD29 pKa = 3.09 LTQIITFSGIFAPLQDD45 pKa = 3.71 PAYY48 pKa = 9.35 FATVRR53 pKa = 11.84 VNPDD57 pKa = 2.75 LGTIVWDD64 pKa = 3.78 NGADD68 pKa = 3.91 LDD70 pKa = 4.6 PDD72 pKa = 3.57 VLYY75 pKa = 10.85 AIVTQQPIPDD85 pKa = 3.89 YY86 pKa = 11.28 TDD88 pKa = 3.73 LQPLNPP94 pKa = 4.36
Molecular weight: 10.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.783
IPC_protein 3.745
Toseland 3.528
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.783
Rodwell 3.579
Grimsley 3.439
Solomon 3.757
Lehninger 3.719
Nozaki 3.91
DTASelect 4.202
Thurlkill 3.617
EMBOSS 3.783
Sillero 3.884
Patrickios 1.914
IPC_peptide 3.745
IPC2_peptide 3.846
IPC2.peptide.svr19 3.797
Protein with the highest isoelectric point:
>tr|A0A2T1D5S6|A0A2T1D5S6_9CYAN DUF3370 domain-containing protein OS=Phormidesmis priestleyi ULC007 OX=1920490 GN=C7B65_23490 PE=4 SV=1
MM1 pKa = 7.56 TKK3 pKa = 9.03 RR4 pKa = 11.84 TLEE7 pKa = 3.89 GTSRR11 pKa = 11.84 KK12 pKa = 9.18 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 KK16 pKa = 8.74 SGFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TATGRR29 pKa = 11.84 RR30 pKa = 11.84 VITARR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.23 KK38 pKa = 9.9 GRR40 pKa = 11.84 ARR42 pKa = 11.84 LSVSGG47 pKa = 3.91
Molecular weight: 5.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.469
IPC2_protein 11.023
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.427
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.149
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.115
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5141
0
5141
1552174
26
3751
301.9
33.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.856 ± 0.033
0.95 ± 0.01
5.28 ± 0.027
5.93 ± 0.035
3.812 ± 0.023
6.727 ± 0.033
1.921 ± 0.019
6.101 ± 0.025
4.148 ± 0.028
11.087 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.892 ± 0.016
3.609 ± 0.024
4.913 ± 0.032
5.532 ± 0.034
5.779 ± 0.025
6.621 ± 0.029
5.801 ± 0.029
6.861 ± 0.029
1.43 ± 0.015
2.75 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here