Mycobacterium phage MooMoo

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1JR78|A0A2P1JR78_9CAUD Minor tail protein OS=Mycobacterium phage MooMoo OX=2108127 GN=17 PE=4 SV=1
MM1 pKa = 8.22DD2 pKa = 4.66EE3 pKa = 5.26GGDD6 pKa = 3.71LTEE9 pKa = 4.27LAGVAEE15 pKa = 5.52ADD17 pKa = 3.79TQAAFAWSEE26 pKa = 4.12AEE28 pKa = 5.08DD29 pKa = 4.18YY30 pKa = 10.81PEE32 pKa = 4.82PADD35 pKa = 3.81SGWPFWVTAAAVGVSLSLVTVAVVLGYY62 pKa = 9.38QHH64 pKa = 7.1LGDD67 pKa = 3.61TSEE70 pKa = 4.62SVVAAAPSTTSTTTSPPAPVAAPSVPPPPPVTVTTVVVQPPPPPVPSPLPPARR123 pKa = 11.84SLASYY128 pKa = 10.52DD129 pKa = 3.81GEE131 pKa = 4.22LLATLRR137 pKa = 11.84SQGWVLPNPAATIDD151 pKa = 3.89DD152 pKa = 3.62AHH154 pKa = 7.15AICLMLRR161 pKa = 11.84QGKK164 pKa = 9.11SRR166 pKa = 11.84AEE168 pKa = 4.07TEE170 pKa = 4.23VIYY173 pKa = 11.02ADD175 pKa = 3.21AAGRR179 pKa = 11.84SVVEE183 pKa = 3.78VSPFIGTIIDD193 pKa = 4.25TYY195 pKa = 10.35PNCPP199 pKa = 3.39

Molecular weight: 20.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1JRG6|A0A2P1JRG6_9CAUD Uncharacterized protein OS=Mycobacterium phage MooMoo OX=2108127 GN=87 PE=4 SV=1
MM1 pKa = 7.7RR2 pKa = 11.84WNIRR6 pKa = 11.84KK7 pKa = 9.5RR8 pKa = 11.84EE9 pKa = 3.95NLLSLPWVVHH19 pKa = 6.29APDD22 pKa = 4.52GSWAGGYY29 pKa = 10.34ASWRR33 pKa = 11.84EE34 pKa = 3.96AMASTSVVSQLEE46 pKa = 3.62AWLRR50 pKa = 11.84TRR52 pKa = 11.84TT53 pKa = 3.61

Molecular weight: 6.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

17618

36

1238

181.6

19.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.216 ± 0.479

1.078 ± 0.156

6.3 ± 0.226

6.073 ± 0.346

2.849 ± 0.127

8.928 ± 0.62

2.14 ± 0.183

4.552 ± 0.209

3.655 ± 0.223

7.572 ± 0.266

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.12

3.196 ± 0.189

6.187 ± 0.219

3.406 ± 0.259

6.573 ± 0.398

5.693 ± 0.291

6.51 ± 0.36

7.203 ± 0.308

2.185 ± 0.154

2.497 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski