Amino acid dipepetide frequency for Mycobacterium phage MooMoo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.326AlaAla: 15.326 ± 1.406
0.795AlaCys: 0.795 ± 0.314
7.322AlaAsp: 7.322 ± 0.74
7.89AlaGlu: 7.89 ± 0.854
2.952AlaPhe: 2.952 ± 0.404
10.161AlaGly: 10.161 ± 1.335
1.589AlaHis: 1.589 ± 0.246
4.711AlaIle: 4.711 ± 0.561
3.973AlaLys: 3.973 ± 0.384
8.798AlaLeu: 8.798 ± 0.826
2.611AlaMet: 2.611 ± 0.429
4.2AlaAsn: 4.2 ± 0.461
5.052AlaPro: 5.052 ± 0.975
4.541AlaGln: 4.541 ± 0.666
6.812AlaArg: 6.812 ± 0.729
6.414AlaSer: 6.414 ± 0.73
6.13AlaThr: 6.13 ± 0.598
7.89AlaVal: 7.89 ± 0.712
2.157AlaTrp: 2.157 ± 0.336
2.781AlaTyr: 2.781 ± 0.391
0.0AlaXaa: 0.0 ± 0.0
Cys
0.851CysAla: 0.851 ± 0.219
0.0CysCys: 0.0 ± 0.0
0.624CysAsp: 0.624 ± 0.165
0.624CysGlu: 0.624 ± 0.179
0.227CysPhe: 0.227 ± 0.109
1.306CysGly: 1.306 ± 0.328
0.397CysHis: 0.397 ± 0.147
0.568CysIle: 0.568 ± 0.168
0.227CysLys: 0.227 ± 0.106
0.908CysLeu: 0.908 ± 0.23
0.114CysMet: 0.114 ± 0.078
0.511CysAsn: 0.511 ± 0.181
0.851CysPro: 0.851 ± 0.236
0.341CysGln: 0.341 ± 0.175
0.795CysArg: 0.795 ± 0.235
0.681CysSer: 0.681 ± 0.179
0.681CysThr: 0.681 ± 0.207
0.624CysVal: 0.624 ± 0.201
0.227CysTrp: 0.227 ± 0.127
0.227CysTyr: 0.227 ± 0.103
0.0CysXaa: 0.0 ± 0.0
Asp
7.55AspAla: 7.55 ± 0.888
0.795AspCys: 0.795 ± 0.222
4.2AspAsp: 4.2 ± 0.589
4.144AspGlu: 4.144 ± 0.505
1.93AspPhe: 1.93 ± 0.303
5.222AspGly: 5.222 ± 0.662
1.362AspHis: 1.362 ± 0.294
2.668AspIle: 2.668 ± 0.408
1.816AspLys: 1.816 ± 0.26
5.903AspLeu: 5.903 ± 0.54
1.192AspMet: 1.192 ± 0.313
1.646AspAsn: 1.646 ± 0.384
4.541AspPro: 4.541 ± 0.486
2.611AspGln: 2.611 ± 0.309
4.257AspArg: 4.257 ± 0.545
4.03AspSer: 4.03 ± 0.448
3.008AspThr: 3.008 ± 0.341
4.087AspVal: 4.087 ± 0.438
1.022AspTrp: 1.022 ± 0.218
1.022AspTyr: 1.022 ± 0.223
0.0AspXaa: 0.0 ± 0.0
Glu
7.095GluAla: 7.095 ± 0.706
0.681GluCys: 0.681 ± 0.192
3.463GluAsp: 3.463 ± 0.355
3.519GluGlu: 3.519 ± 0.563
2.327GluPhe: 2.327 ± 0.398
2.554GluGly: 2.554 ± 0.412
1.533GluHis: 1.533 ± 0.31
2.271GluIle: 2.271 ± 0.341
2.725GluLys: 2.725 ± 0.552
5.733GluLeu: 5.733 ± 0.636
1.646GluMet: 1.646 ± 0.301
1.589GluAsn: 1.589 ± 0.219
2.952GluPro: 2.952 ± 0.413
2.668GluGln: 2.668 ± 0.415
4.995GluArg: 4.995 ± 0.644
3.292GluSer: 3.292 ± 0.375
3.576GluThr: 3.576 ± 0.363
4.541GluVal: 4.541 ± 0.585
1.533GluTrp: 1.533 ± 0.313
2.043GluTyr: 2.043 ± 0.37
0.0GluXaa: 0.0 ± 0.0
Phe
3.065PheAla: 3.065 ± 0.459
0.227PheCys: 0.227 ± 0.122
2.1PheAsp: 2.1 ± 0.307
1.93PheGlu: 1.93 ± 0.313
0.511PhePhe: 0.511 ± 0.168
3.122PheGly: 3.122 ± 0.454
0.568PheHis: 0.568 ± 0.168
1.192PheIle: 1.192 ± 0.3
1.249PheLys: 1.249 ± 0.294
1.873PheLeu: 1.873 ± 0.347
0.795PheMet: 0.795 ± 0.204
0.908PheAsn: 0.908 ± 0.237
1.419PhePro: 1.419 ± 0.284
1.022PheGln: 1.022 ± 0.235
1.419PheArg: 1.419 ± 0.197
1.646PheSer: 1.646 ± 0.244
2.327PheThr: 2.327 ± 0.435
1.703PheVal: 1.703 ± 0.343
0.795PheTrp: 0.795 ± 0.223
0.624PheTyr: 0.624 ± 0.176
0.0PheXaa: 0.0 ± 0.0
Gly
9.536GlyAla: 9.536 ± 1.285
0.908GlyCys: 0.908 ± 0.254
4.938GlyAsp: 4.938 ± 0.528
5.563GlyGlu: 5.563 ± 0.541
2.668GlyPhe: 2.668 ± 0.407
10.899GlyGly: 10.899 ± 2.72
2.441GlyHis: 2.441 ± 0.344
4.144GlyIle: 4.144 ± 0.537
3.576GlyLys: 3.576 ± 0.4
5.393GlyLeu: 5.393 ± 0.686
1.533GlyMet: 1.533 ± 0.276
2.611GlyAsn: 2.611 ± 0.477
4.144GlyPro: 4.144 ± 0.585
2.725GlyGln: 2.725 ± 0.58
4.882GlyArg: 4.882 ± 0.548
5.165GlySer: 5.165 ± 0.927
6.812GlyThr: 6.812 ± 0.67
6.698GlyVal: 6.698 ± 0.608
2.214GlyTrp: 2.214 ± 0.38
2.441GlyTyr: 2.441 ± 0.377
0.0GlyXaa: 0.0 ± 0.0
His
1.93HisAla: 1.93 ± 0.357
0.341HisCys: 0.341 ± 0.158
1.192HisAsp: 1.192 ± 0.257
0.965HisGlu: 0.965 ± 0.211
0.511HisPhe: 0.511 ± 0.163
2.043HisGly: 2.043 ± 0.326
0.965HisHis: 0.965 ± 0.275
1.022HisIle: 1.022 ± 0.279
0.795HisLys: 0.795 ± 0.21
1.987HisLeu: 1.987 ± 0.36
0.568HisMet: 0.568 ± 0.174
0.738HisAsn: 0.738 ± 0.241
1.703HisPro: 1.703 ± 0.302
0.681HisGln: 0.681 ± 0.182
2.1HisArg: 2.1 ± 0.374
0.738HisSer: 0.738 ± 0.185
1.249HisThr: 1.249 ± 0.315
1.022HisVal: 1.022 ± 0.237
0.454HisTrp: 0.454 ± 0.142
0.397HisTyr: 0.397 ± 0.13
0.0HisXaa: 0.0 ± 0.0
Ile
5.62IleAla: 5.62 ± 0.526
0.227IleCys: 0.227 ± 0.112
4.257IleAsp: 4.257 ± 0.564
3.008IleGlu: 3.008 ± 0.306
0.568IlePhe: 0.568 ± 0.22
3.917IleGly: 3.917 ± 0.457
0.851IleHis: 0.851 ± 0.21
2.384IleIle: 2.384 ± 0.588
1.646IleLys: 1.646 ± 0.306
2.668IleLeu: 2.668 ± 0.442
0.511IleMet: 0.511 ± 0.17
1.589IleAsn: 1.589 ± 0.295
3.463IlePro: 3.463 ± 0.458
1.249IleGln: 1.249 ± 0.269
2.554IleArg: 2.554 ± 0.334
2.554IleSer: 2.554 ± 0.415
4.03IleThr: 4.03 ± 0.705
3.008IleVal: 3.008 ± 0.37
0.568IleTrp: 0.568 ± 0.173
0.851IleTyr: 0.851 ± 0.213
0.0IleXaa: 0.0 ± 0.0
Lys
4.314LysAla: 4.314 ± 0.491
0.511LysCys: 0.511 ± 0.196
2.157LysAsp: 2.157 ± 0.391
1.589LysGlu: 1.589 ± 0.319
1.533LysPhe: 1.533 ± 0.304
2.895LysGly: 2.895 ± 0.464
0.795LysHis: 0.795 ± 0.259
1.646LysIle: 1.646 ± 0.342
1.533LysLys: 1.533 ± 0.403
3.122LysLeu: 3.122 ± 0.458
0.454LysMet: 0.454 ± 0.171
0.965LysAsn: 0.965 ± 0.208
2.611LysPro: 2.611 ± 0.441
1.135LysGln: 1.135 ± 0.266
2.1LysArg: 2.1 ± 0.324
1.646LysSer: 1.646 ± 0.305
2.441LysThr: 2.441 ± 0.34
2.781LysVal: 2.781 ± 0.418
1.135LysTrp: 1.135 ± 0.249
1.192LysTyr: 1.192 ± 0.314
0.0LysXaa: 0.0 ± 0.0
Leu
9.763LeuAla: 9.763 ± 0.918
0.568LeuCys: 0.568 ± 0.175
5.109LeuAsp: 5.109 ± 0.625
4.257LeuGlu: 4.257 ± 0.449
1.987LeuPhe: 1.987 ± 0.331
6.074LeuGly: 6.074 ± 0.618
1.646LeuHis: 1.646 ± 0.31
3.633LeuIle: 3.633 ± 0.427
2.895LeuLys: 2.895 ± 0.431
5.62LeuLeu: 5.62 ± 0.47
1.589LeuMet: 1.589 ± 0.29
2.952LeuAsn: 2.952 ± 0.54
4.938LeuPro: 4.938 ± 0.71
2.838LeuGln: 2.838 ± 0.314
4.938LeuArg: 4.938 ± 0.62
3.917LeuSer: 3.917 ± 0.495
4.711LeuThr: 4.711 ± 0.574
5.393LeuVal: 5.393 ± 0.611
1.192LeuTrp: 1.192 ± 0.329
1.703LeuTyr: 1.703 ± 0.301
0.0LeuXaa: 0.0 ± 0.0
Met
2.327MetAla: 2.327 ± 0.336
0.17MetCys: 0.17 ± 0.103
0.851MetAsp: 0.851 ± 0.239
0.851MetGlu: 0.851 ± 0.221
0.341MetPhe: 0.341 ± 0.12
1.533MetGly: 1.533 ± 0.361
0.341MetHis: 0.341 ± 0.17
1.135MetIle: 1.135 ± 0.228
1.249MetLys: 1.249 ± 0.275
1.533MetLeu: 1.533 ± 0.278
0.397MetMet: 0.397 ± 0.123
0.908MetAsn: 0.908 ± 0.243
1.476MetPro: 1.476 ± 0.26
0.511MetGln: 0.511 ± 0.186
1.816MetArg: 1.816 ± 0.318
2.271MetSer: 2.271 ± 0.331
2.327MetThr: 2.327 ± 0.392
0.965MetVal: 0.965 ± 0.266
0.397MetTrp: 0.397 ± 0.134
0.454MetTyr: 0.454 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
3.349AsnAla: 3.349 ± 0.443
0.511AsnCys: 0.511 ± 0.153
1.873AsnAsp: 1.873 ± 0.325
1.873AsnGlu: 1.873 ± 0.294
0.681AsnPhe: 0.681 ± 0.209
3.576AsnGly: 3.576 ± 0.478
0.284AsnHis: 0.284 ± 0.123
1.476AsnIle: 1.476 ± 0.288
1.306AsnLys: 1.306 ± 0.241
2.384AsnLeu: 2.384 ± 0.338
0.397AsnMet: 0.397 ± 0.124
1.192AsnAsn: 1.192 ± 0.269
3.122AsnPro: 3.122 ± 0.37
0.908AsnGln: 0.908 ± 0.287
2.668AsnArg: 2.668 ± 0.414
1.646AsnSer: 1.646 ± 0.293
2.043AsnThr: 2.043 ± 0.372
1.816AsnVal: 1.816 ± 0.378
0.284AsnTrp: 0.284 ± 0.127
0.568AsnTyr: 0.568 ± 0.197
0.0AsnXaa: 0.0 ± 0.0
Pro
6.698ProAla: 6.698 ± 0.85
0.284ProCys: 0.284 ± 0.116
4.598ProAsp: 4.598 ± 0.644
4.03ProGlu: 4.03 ± 0.455
1.306ProPhe: 1.306 ± 0.289
6.244ProGly: 6.244 ± 0.697
1.476ProHis: 1.476 ± 0.345
2.554ProIle: 2.554 ± 0.326
2.271ProLys: 2.271 ± 0.432
3.973ProLeu: 3.973 ± 0.484
2.157ProMet: 2.157 ± 0.35
2.214ProAsn: 2.214 ± 0.301
4.257ProPro: 4.257 ± 0.75
1.873ProGln: 1.873 ± 0.389
2.668ProArg: 2.668 ± 0.502
3.86ProSer: 3.86 ± 0.426
3.576ProThr: 3.576 ± 0.609
4.882ProVal: 4.882 ± 0.59
1.306ProTrp: 1.306 ± 0.319
1.646ProTyr: 1.646 ± 0.361
0.0ProXaa: 0.0 ± 0.0
Gln
3.973GlnAla: 3.973 ± 0.433
0.17GlnCys: 0.17 ± 0.101
1.703GlnAsp: 1.703 ± 0.259
1.419GlnGlu: 1.419 ± 0.268
1.249GlnPhe: 1.249 ± 0.227
2.554GlnGly: 2.554 ± 0.699
1.022GlnHis: 1.022 ± 0.221
1.476GlnIle: 1.476 ± 0.218
1.419GlnLys: 1.419 ± 0.272
2.725GlnLeu: 2.725 ± 0.376
0.908GlnMet: 0.908 ± 0.224
1.022GlnAsn: 1.022 ± 0.203
2.554GlnPro: 2.554 ± 0.356
1.476GlnGln: 1.476 ± 0.455
2.441GlnArg: 2.441 ± 0.366
1.703GlnSer: 1.703 ± 0.304
1.419GlnThr: 1.419 ± 0.289
2.838GlnVal: 2.838 ± 0.322
0.965GlnTrp: 0.965 ± 0.231
1.022GlnTyr: 1.022 ± 0.306
0.0GlnXaa: 0.0 ± 0.0
Arg
6.585ArgAla: 6.585 ± 0.557
1.306ArgCys: 1.306 ± 0.301
3.803ArgAsp: 3.803 ± 0.473
4.257ArgGlu: 4.257 ± 0.621
2.043ArgPhe: 2.043 ± 0.346
4.484ArgGly: 4.484 ± 0.434
1.419ArgHis: 1.419 ± 0.288
3.179ArgIle: 3.179 ± 0.429
2.271ArgLys: 2.271 ± 0.39
4.711ArgLeu: 4.711 ± 0.609
1.362ArgMet: 1.362 ± 0.264
2.327ArgAsn: 2.327 ± 0.457
3.576ArgPro: 3.576 ± 0.45
2.611ArgGln: 2.611 ± 0.411
5.279ArgArg: 5.279 ± 0.74
3.746ArgSer: 3.746 ± 0.525
4.541ArgThr: 4.541 ± 0.639
4.371ArgVal: 4.371 ± 0.576
1.703ArgTrp: 1.703 ± 0.369
2.157ArgTyr: 2.157 ± 0.412
0.0ArgXaa: 0.0 ± 0.0
Ser
4.882SerAla: 4.882 ± 0.596
0.681SerCys: 0.681 ± 0.206
3.406SerAsp: 3.406 ± 0.346
3.463SerGlu: 3.463 ± 0.455
1.703SerPhe: 1.703 ± 0.328
6.812SerGly: 6.812 ± 0.969
1.022SerHis: 1.022 ± 0.263
3.179SerIle: 3.179 ± 0.455
1.419SerLys: 1.419 ± 0.277
3.746SerLeu: 3.746 ± 0.423
1.873SerMet: 1.873 ± 0.348
1.533SerAsn: 1.533 ± 0.355
3.065SerPro: 3.065 ± 0.379
1.873SerGln: 1.873 ± 0.349
3.803SerArg: 3.803 ± 0.477
3.406SerSer: 3.406 ± 0.601
3.292SerThr: 3.292 ± 0.482
4.655SerVal: 4.655 ± 0.473
1.476SerTrp: 1.476 ± 0.278
1.646SerTyr: 1.646 ± 0.34
0.0SerXaa: 0.0 ± 0.0
Thr
6.471ThrAla: 6.471 ± 0.819
0.511ThrCys: 0.511 ± 0.175
3.349ThrAsp: 3.349 ± 0.367
3.576ThrGlu: 3.576 ± 0.533
2.157ThrPhe: 2.157 ± 0.367
6.585ThrGly: 6.585 ± 0.796
1.022ThrHis: 1.022 ± 0.285
3.633ThrIle: 3.633 ± 0.665
2.725ThrLys: 2.725 ± 0.415
4.541ThrLeu: 4.541 ± 0.572
1.022ThrMet: 1.022 ± 0.21
2.157ThrAsn: 2.157 ± 0.38
5.79ThrPro: 5.79 ± 0.652
1.646ThrGln: 1.646 ± 0.337
3.803ThrArg: 3.803 ± 0.524
3.179ThrSer: 3.179 ± 0.615
4.598ThrThr: 4.598 ± 0.671
5.279ThrVal: 5.279 ± 0.652
1.533ThrTrp: 1.533 ± 0.29
1.533ThrTyr: 1.533 ± 0.262
0.0ThrXaa: 0.0 ± 0.0
Val
7.947ValAla: 7.947 ± 0.517
1.419ValCys: 1.419 ± 0.331
4.768ValAsp: 4.768 ± 0.517
4.882ValGlu: 4.882 ± 0.543
2.043ValPhe: 2.043 ± 0.321
5.676ValGly: 5.676 ± 0.651
1.362ValHis: 1.362 ± 0.273
3.008ValIle: 3.008 ± 0.413
1.816ValLys: 1.816 ± 0.367
6.357ValLeu: 6.357 ± 0.734
1.873ValMet: 1.873 ± 0.365
1.703ValAsn: 1.703 ± 0.31
3.406ValPro: 3.406 ± 0.413
1.76ValGln: 1.76 ± 0.288
4.825ValArg: 4.825 ± 0.679
4.655ValSer: 4.655 ± 0.47
5.563ValThr: 5.563 ± 0.556
5.62ValVal: 5.62 ± 0.711
1.533ValTrp: 1.533 ± 0.306
1.816ValTyr: 1.816 ± 0.305
0.0ValXaa: 0.0 ± 0.0
Trp
1.703TrpAla: 1.703 ± 0.352
0.454TrpCys: 0.454 ± 0.147
1.362TrpAsp: 1.362 ± 0.32
1.135TrpGlu: 1.135 ± 0.218
0.681TrpPhe: 0.681 ± 0.192
1.249TrpGly: 1.249 ± 0.253
0.795TrpHis: 0.795 ± 0.175
0.908TrpIle: 0.908 ± 0.262
1.079TrpLys: 1.079 ± 0.205
1.93TrpLeu: 1.93 ± 0.345
0.568TrpMet: 0.568 ± 0.16
0.795TrpAsn: 0.795 ± 0.212
1.249TrpPro: 1.249 ± 0.246
0.908TrpGln: 0.908 ± 0.267
1.476TrpArg: 1.476 ± 0.316
1.249TrpSer: 1.249 ± 0.261
1.135TrpThr: 1.135 ± 0.303
1.873TrpVal: 1.873 ± 0.359
0.965TrpTrp: 0.965 ± 0.271
0.341TrpTyr: 0.341 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.179TyrAla: 3.179 ± 0.398
0.227TyrCys: 0.227 ± 0.095
1.93TyrAsp: 1.93 ± 0.247
1.76TyrGlu: 1.76 ± 0.213
1.079TyrPhe: 1.079 ± 0.224
2.384TyrGly: 2.384 ± 0.36
0.511TyrHis: 0.511 ± 0.142
0.738TyrIle: 0.738 ± 0.233
0.568TyrLys: 0.568 ± 0.148
1.816TyrLeu: 1.816 ± 0.338
0.227TyrMet: 0.227 ± 0.136
0.397TyrAsn: 0.397 ± 0.148
1.703TyrPro: 1.703 ± 0.348
0.681TyrGln: 0.681 ± 0.192
2.1TyrArg: 2.1 ± 0.356
1.135TyrSer: 1.135 ± 0.251
1.646TyrThr: 1.646 ± 0.291
1.987TyrVal: 1.987 ± 0.454
0.397TyrTrp: 0.397 ± 0.16
0.511TyrTyr: 0.511 ± 0.175
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 97 proteins (17618 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski