Streptococcus massiliensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Streptococcus

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1899 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A380L194|A0A380L194_9STRE 3-oxoacyl-[acyl-carrier-protein] synthase 2 OS=Streptococcus massiliensis OX=313439 GN=fabF PE=3 SV=1
MM1 pKa = 7.15AHH3 pKa = 6.09EE4 pKa = 5.17HH5 pKa = 5.86NHH7 pKa = 5.89DD8 pKa = 3.7HH9 pKa = 7.19DD10 pKa = 3.87HH11 pKa = 7.4AEE13 pKa = 4.01RR14 pKa = 11.84DD15 pKa = 3.92LITLVDD21 pKa = 3.8EE22 pKa = 5.11NGNEE26 pKa = 4.12TLFEE30 pKa = 4.24ILLTIDD36 pKa = 3.07GMEE39 pKa = 4.14EE40 pKa = 3.64FGKK43 pKa = 10.47NYY45 pKa = 9.61VLLMPVHH52 pKa = 7.31AEE54 pKa = 3.57EE55 pKa = 5.22DD56 pKa = 3.71EE57 pKa = 4.33NGEE60 pKa = 4.24VEE62 pKa = 4.14IQAYY66 pKa = 10.22SYY68 pKa = 11.44VEE70 pKa = 4.22NEE72 pKa = 4.81DD73 pKa = 3.37GTEE76 pKa = 4.12GDD78 pKa = 4.72LEE80 pKa = 5.05PIPEE84 pKa = 4.74DD85 pKa = 5.05SDD87 pKa = 4.93NEE89 pKa = 3.57WDD91 pKa = 3.74MIEE94 pKa = 4.19EE95 pKa = 4.26VFNSFMEE102 pKa = 4.25EE103 pKa = 3.85AEE105 pKa = 4.07

Molecular weight: 12.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A380KUV1|A0A380KUV1_9STRE Transposase OS=Streptococcus massiliensis OX=313439 GN=NCTC13765_00126 PE=4 SV=1
MM1 pKa = 7.92PKK3 pKa = 10.14QKK5 pKa = 7.59THH7 pKa = 6.46RR8 pKa = 11.84ASAKK12 pKa = 8.58RR13 pKa = 11.84FKK15 pKa = 10.11RR16 pKa = 11.84TGSGGLKK23 pKa = 10.04RR24 pKa = 11.84FRR26 pKa = 11.84AYY28 pKa = 9.4TSHH31 pKa = 7.17RR32 pKa = 11.84FHH34 pKa = 7.37GKK36 pKa = 6.22TKK38 pKa = 9.47KK39 pKa = 9.37QRR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.45LRR45 pKa = 11.84KK46 pKa = 9.43AAMVSAGDD54 pKa = 3.8FKK56 pKa = 10.94RR57 pKa = 11.84IKK59 pKa = 11.07AMLTGLKK66 pKa = 10.19

Molecular weight: 7.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1899

0

1899

577382

30

2783

304.0

34.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.864 ± 0.062

0.577 ± 0.016

5.499 ± 0.046

7.083 ± 0.07

4.607 ± 0.052

6.431 ± 0.055

1.958 ± 0.024

7.14 ± 0.054

7.035 ± 0.064

10.274 ± 0.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.462 ± 0.028

4.421 ± 0.04

3.265 ± 0.029

4.204 ± 0.042

4.207 ± 0.05

5.914 ± 0.043

5.603 ± 0.065

6.84 ± 0.05

0.858 ± 0.02

3.759 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski