Amino acid dipepetide frequency for Streptococcus massiliensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.772AlaAla: 6.772 ± 0.147
0.544AlaCys: 0.544 ± 0.032
4.276AlaAsp: 4.276 ± 0.096
5.643AlaGlu: 5.643 ± 0.115
3.473AlaPhe: 3.473 ± 0.089
6.0AlaGly: 6.0 ± 0.127
1.379AlaHis: 1.379 ± 0.053
5.599AlaIle: 5.599 ± 0.108
5.26AlaLys: 5.26 ± 0.091
7.936AlaLeu: 7.936 ± 0.125
1.858AlaMet: 1.858 ± 0.059
3.071AlaAsn: 3.071 ± 0.083
2.264AlaPro: 2.264 ± 0.076
3.525AlaGln: 3.525 ± 0.116
3.27AlaArg: 3.27 ± 0.076
4.482AlaSer: 4.482 ± 0.099
4.584AlaThr: 4.584 ± 0.093
5.395AlaVal: 5.395 ± 0.12
0.613AlaTrp: 0.613 ± 0.035
2.698AlaTyr: 2.698 ± 0.07
0.0AlaXaa: 0.0 ± 0.0
Cys
0.48CysAla: 0.48 ± 0.027
0.045CysCys: 0.045 ± 0.009
0.303CysAsp: 0.303 ± 0.025
0.256CysGlu: 0.256 ± 0.019
0.242CysPhe: 0.242 ± 0.02
0.48CysGly: 0.48 ± 0.034
0.203CysHis: 0.203 ± 0.022
0.355CysIle: 0.355 ± 0.024
0.274CysLys: 0.274 ± 0.02
0.688CysLeu: 0.688 ± 0.04
0.111CysMet: 0.111 ± 0.014
0.236CysAsn: 0.236 ± 0.018
0.251CysPro: 0.251 ± 0.023
0.372CysGln: 0.372 ± 0.026
0.284CysArg: 0.284 ± 0.025
0.352CysSer: 0.352 ± 0.025
0.229CysThr: 0.229 ± 0.022
0.307CysVal: 0.307 ± 0.024
0.035CysTrp: 0.035 ± 0.008
0.265CysTyr: 0.265 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
3.59AspAla: 3.59 ± 0.093
0.424AspCys: 0.424 ± 0.032
2.685AspAsp: 2.685 ± 0.091
4.115AspGlu: 4.115 ± 0.099
3.48AspPhe: 3.48 ± 0.091
3.583AspGly: 3.583 ± 0.105
0.89AspHis: 0.89 ± 0.041
3.89AspIle: 3.89 ± 0.092
4.403AspLys: 4.403 ± 0.107
6.006AspLeu: 6.006 ± 0.116
1.528AspMet: 1.528 ± 0.05
2.236AspAsn: 2.236 ± 0.065
1.505AspPro: 1.505 ± 0.057
1.978AspGln: 1.978 ± 0.062
1.959AspArg: 1.959 ± 0.057
2.936AspSer: 2.936 ± 0.078
2.622AspThr: 2.622 ± 0.079
3.677AspVal: 3.677 ± 0.078
0.617AspTrp: 0.617 ± 0.038
2.863AspTyr: 2.863 ± 0.068
0.0AspXaa: 0.0 ± 0.0
Glu
5.373GluAla: 5.373 ± 0.109
0.352GluCys: 0.352 ± 0.026
3.604GluAsp: 3.604 ± 0.081
5.986GluGlu: 5.986 ± 0.152
2.594GluPhe: 2.594 ± 0.079
3.592GluGly: 3.592 ± 0.085
1.585GluHis: 1.585 ± 0.053
5.317GluIle: 5.317 ± 0.096
6.526GluLys: 6.526 ± 0.139
7.236GluLeu: 7.236 ± 0.128
1.746GluMet: 1.746 ± 0.048
4.157GluAsn: 4.157 ± 0.1
1.749GluPro: 1.749 ± 0.053
3.37GluGln: 3.37 ± 0.096
3.58GluArg: 3.58 ± 0.096
3.171GluSer: 3.171 ± 0.08
3.786GluThr: 3.786 ± 0.082
4.581GluVal: 4.581 ± 0.101
0.528GluTrp: 0.528 ± 0.033
1.997GluTyr: 1.997 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
3.63PheAla: 3.63 ± 0.1
0.256PheCys: 0.256 ± 0.024
3.0PheAsp: 3.0 ± 0.078
3.012PheGlu: 3.012 ± 0.073
2.137PhePhe: 2.137 ± 0.067
3.239PheGly: 3.239 ± 0.078
0.961PheHis: 0.961 ± 0.04
3.221PheIle: 3.221 ± 0.088
2.64PheLys: 2.64 ± 0.076
4.754PheLeu: 4.754 ± 0.132
1.022PheMet: 1.022 ± 0.044
1.789PheAsn: 1.789 ± 0.052
1.529PhePro: 1.529 ± 0.047
1.742PheGln: 1.742 ± 0.061
1.706PheArg: 1.706 ± 0.057
3.41PheSer: 3.41 ± 0.092
2.451PheThr: 2.451 ± 0.08
3.178PheVal: 3.178 ± 0.077
0.457PheTrp: 0.457 ± 0.029
1.931PheTyr: 1.931 ± 0.072
0.0PheXaa: 0.0 ± 0.0
Gly
4.718GlyAla: 4.718 ± 0.11
0.381GlyCys: 0.381 ± 0.027
3.128GlyAsp: 3.128 ± 0.079
3.592GlyGlu: 3.592 ± 0.079
3.014GlyPhe: 3.014 ± 0.074
4.164GlyGly: 4.164 ± 0.115
1.271GlyHis: 1.271 ± 0.049
4.938GlyIle: 4.938 ± 0.103
4.773GlyLys: 4.773 ± 0.098
7.004GlyLeu: 7.004 ± 0.117
1.735GlyMet: 1.735 ± 0.053
2.688GlyAsn: 2.688 ± 0.074
1.285GlyPro: 1.285 ± 0.052
3.365GlyGln: 3.365 ± 0.079
2.906GlyArg: 2.906 ± 0.077
3.551GlySer: 3.551 ± 0.082
3.693GlyThr: 3.693 ± 0.081
4.718GlyVal: 4.718 ± 0.095
0.627GlyTrp: 0.627 ± 0.034
2.756GlyTyr: 2.756 ± 0.068
0.0GlyXaa: 0.0 ± 0.0
His
1.271HisAla: 1.271 ± 0.053
0.159HisCys: 0.159 ± 0.016
0.985HisAsp: 0.985 ± 0.039
1.159HisGlu: 1.159 ± 0.047
1.304HisPhe: 1.304 ± 0.055
1.275HisGly: 1.275 ± 0.045
0.525HisHis: 0.525 ± 0.03
1.328HisIle: 1.328 ± 0.046
1.1HisLys: 1.1 ± 0.037
2.309HisLeu: 2.309 ± 0.068
0.533HisMet: 0.533 ± 0.029
0.684HisAsn: 0.684 ± 0.032
0.928HisPro: 0.928 ± 0.04
0.918HisGln: 0.918 ± 0.042
0.819HisArg: 0.819 ± 0.037
0.996HisSer: 0.996 ± 0.042
0.902HisThr: 0.902 ± 0.042
1.152HisVal: 1.152 ± 0.043
0.147HisTrp: 0.147 ± 0.017
1.086HisTyr: 1.086 ± 0.047
0.0HisXaa: 0.0 ± 0.0
Ile
6.301IleAla: 6.301 ± 0.13
0.566IleCys: 0.566 ± 0.03
3.996IleAsp: 3.996 ± 0.075
5.049IleGlu: 5.049 ± 0.11
3.528IlePhe: 3.528 ± 0.081
4.569IleGly: 4.569 ± 0.112
1.346IleHis: 1.346 ± 0.047
4.917IleIle: 4.917 ± 0.112
4.631IleLys: 4.631 ± 0.096
7.33IleLeu: 7.33 ± 0.143
1.654IleMet: 1.654 ± 0.055
3.0IleAsn: 3.0 ± 0.068
2.6IlePro: 2.6 ± 0.065
2.518IleGln: 2.518 ± 0.058
3.008IleArg: 3.008 ± 0.073
4.621IleSer: 4.621 ± 0.102
3.772IleThr: 3.772 ± 0.103
4.82IleVal: 4.82 ± 0.108
0.591IleTrp: 0.591 ± 0.035
2.581IleTyr: 2.581 ± 0.075
0.0IleXaa: 0.0 ± 0.0
Lys
4.985LysAla: 4.985 ± 0.101
0.274LysCys: 0.274 ± 0.021
3.978LysAsp: 3.978 ± 0.099
6.595LysGlu: 6.595 ± 0.126
2.158LysPhe: 2.158 ± 0.06
3.909LysGly: 3.909 ± 0.078
1.192LysHis: 1.192 ± 0.047
5.418LysIle: 5.418 ± 0.115
5.819LysLys: 5.819 ± 0.125
6.242LysLeu: 6.242 ± 0.123
2.203LysMet: 2.203 ± 0.062
3.744LysAsn: 3.744 ± 0.094
1.869LysPro: 1.869 ± 0.06
2.674LysGln: 2.674 ± 0.079
3.412LysArg: 3.412 ± 0.085
3.739LysSer: 3.739 ± 0.083
4.243LysThr: 4.243 ± 0.095
4.822LysVal: 4.822 ± 0.11
0.608LysTrp: 0.608 ± 0.037
2.466LysTyr: 2.466 ± 0.079
0.0LysXaa: 0.0 ± 0.0
Leu
9.585LeuAla: 9.585 ± 0.155
0.443LeuCys: 0.443 ± 0.03
5.986LeuAsp: 5.986 ± 0.118
7.283LeuGlu: 7.283 ± 0.136
4.539LeuPhe: 4.539 ± 0.126
6.486LeuGly: 6.486 ± 0.126
1.659LeuHis: 1.659 ± 0.057
6.935LeuIle: 6.935 ± 0.143
6.336LeuLys: 6.336 ± 0.106
10.837LeuLeu: 10.837 ± 0.231
2.343LeuMet: 2.343 ± 0.064
4.061LeuAsn: 4.061 ± 0.086
4.266LeuPro: 4.266 ± 0.078
3.547LeuGln: 3.547 ± 0.085
4.049LeuArg: 4.049 ± 0.099
7.021LeuSer: 7.021 ± 0.111
6.358LeuThr: 6.358 ± 0.121
7.176LeuVal: 7.176 ± 0.126
0.672LeuTrp: 0.672 ± 0.037
3.156LeuTyr: 3.156 ± 0.093
0.0LeuXaa: 0.0 ± 0.0
Met
2.026MetAla: 2.026 ± 0.069
0.125MetCys: 0.125 ± 0.014
1.299MetAsp: 1.299 ± 0.044
1.581MetGlu: 1.581 ± 0.062
0.89MetPhe: 0.89 ± 0.04
1.652MetGly: 1.652 ± 0.055
0.435MetHis: 0.435 ± 0.027
1.94MetIle: 1.94 ± 0.071
2.181MetLys: 2.181 ± 0.06
2.179MetLeu: 2.179 ± 0.061
0.684MetMet: 0.684 ± 0.037
1.254MetAsn: 1.254 ± 0.044
0.798MetPro: 0.798 ± 0.037
0.93MetGln: 0.93 ± 0.039
1.006MetArg: 1.006 ± 0.038
1.611MetSer: 1.611 ± 0.052
1.822MetThr: 1.822 ± 0.056
1.462MetVal: 1.462 ± 0.051
0.163MetTrp: 0.163 ± 0.015
0.584MetTyr: 0.584 ± 0.035
0.0MetXaa: 0.0 ± 0.0
Asn
3.066AsnAla: 3.066 ± 0.074
0.313AsnCys: 0.313 ± 0.024
2.049AsnAsp: 2.049 ± 0.062
2.527AsnGlu: 2.527 ± 0.066
2.329AsnPhe: 2.329 ± 0.075
3.138AsnGly: 3.138 ± 0.097
1.039AsnHis: 1.039 ± 0.051
3.161AsnIle: 3.161 ± 0.088
2.787AsnLys: 2.787 ± 0.077
4.742AsnLeu: 4.742 ± 0.09
1.112AsnMet: 1.112 ± 0.048
1.916AsnAsn: 1.916 ± 0.067
2.21AsnPro: 2.21 ± 0.07
2.359AsnGln: 2.359 ± 0.07
2.096AsnArg: 2.096 ± 0.067
2.47AsnSer: 2.47 ± 0.078
2.033AsnThr: 2.033 ± 0.075
2.615AsnVal: 2.615 ± 0.076
0.49AsnTrp: 0.49 ± 0.033
1.758AsnTyr: 1.758 ± 0.059
0.0AsnXaa: 0.0 ± 0.0
Pro
2.64ProAla: 2.64 ± 0.079
0.125ProCys: 0.125 ± 0.015
2.025ProAsp: 2.025 ± 0.059
2.717ProGlu: 2.717 ± 0.078
1.638ProPhe: 1.638 ± 0.046
1.604ProGly: 1.604 ± 0.055
0.769ProHis: 0.769 ± 0.038
2.38ProIle: 2.38 ± 0.068
2.064ProLys: 2.064 ± 0.067
3.05ProLeu: 3.05 ± 0.078
0.715ProMet: 0.715 ± 0.033
1.564ProAsn: 1.564 ± 0.057
0.559ProPro: 0.559 ± 0.036
1.424ProGln: 1.424 ± 0.049
1.029ProArg: 1.029 ± 0.041
2.104ProSer: 2.104 ± 0.061
2.066ProThr: 2.066 ± 0.068
2.446ProVal: 2.446 ± 0.066
0.268ProTrp: 0.268 ± 0.021
1.46ProTyr: 1.46 ± 0.053
0.0ProXaa: 0.0 ± 0.0
Gln
3.951GlnAla: 3.951 ± 0.101
0.104GlnCys: 0.104 ± 0.014
2.141GlnAsp: 2.141 ± 0.06
3.556GlnGlu: 3.556 ± 0.087
1.528GlnPhe: 1.528 ± 0.054
2.484GlnGly: 2.484 ± 0.057
0.84GlnHis: 0.84 ± 0.037
3.18GlnIle: 3.18 ± 0.064
3.291GlnLys: 3.291 ± 0.084
4.368GlnLeu: 4.368 ± 0.103
1.091GlnMet: 1.091 ± 0.045
1.687GlnAsn: 1.687 ± 0.059
1.273GlnPro: 1.273 ± 0.05
1.574GlnGln: 1.574 ± 0.067
1.469GlnArg: 1.469 ± 0.057
2.044GlnSer: 2.044 ± 0.057
2.368GlnThr: 2.368 ± 0.064
3.547GlnVal: 3.547 ± 0.091
0.291GlnTrp: 0.291 ± 0.027
1.257GlnTyr: 1.257 ± 0.047
0.0GlnXaa: 0.0 ± 0.0
Arg
2.906ArgAla: 2.906 ± 0.074
0.274ArgCys: 0.274 ± 0.024
2.257ArgAsp: 2.257 ± 0.063
3.213ArgGlu: 3.213 ± 0.093
2.269ArgPhe: 2.269 ± 0.07
2.361ArgGly: 2.361 ± 0.07
0.786ArgHis: 0.786 ± 0.042
2.854ArgIle: 2.854 ± 0.068
3.346ArgLys: 3.346 ± 0.103
4.344ArgLeu: 4.344 ± 0.099
1.145ArgMet: 1.145 ± 0.045
2.0ArgAsn: 2.0 ± 0.07
1.325ArgPro: 1.325 ± 0.045
2.092ArgGln: 2.092 ± 0.065
2.013ArgArg: 2.013 ± 0.067
1.959ArgSer: 1.959 ± 0.065
2.106ArgThr: 2.106 ± 0.056
2.775ArgVal: 2.775 ± 0.072
0.279ArgTrp: 0.279 ± 0.022
1.772ArgTyr: 1.772 ± 0.051
0.0ArgXaa: 0.0 ± 0.0
Ser
3.888SerAla: 3.888 ± 0.087
0.345SerCys: 0.345 ± 0.027
3.175SerAsp: 3.175 ± 0.079
3.49SerGlu: 3.49 ± 0.077
2.976SerPhe: 2.976 ± 0.078
4.195SerGly: 4.195 ± 0.084
1.33SerHis: 1.33 ± 0.045
3.874SerIle: 3.874 ± 0.095
4.06SerLys: 4.06 ± 0.086
6.292SerLeu: 6.292 ± 0.133
1.436SerMet: 1.436 ± 0.053
2.546SerAsn: 2.546 ± 0.071
1.928SerPro: 1.928 ± 0.059
2.95SerGln: 2.95 ± 0.083
2.589SerArg: 2.589 ± 0.071
4.049SerSer: 4.049 ± 0.119
2.996SerThr: 2.996 ± 0.075
3.864SerVal: 3.864 ± 0.085
0.608SerTrp: 0.608 ± 0.039
2.551SerTyr: 2.551 ± 0.067
0.0SerXaa: 0.0 ± 0.0
Thr
4.238ThrAla: 4.238 ± 0.095
0.32ThrCys: 0.32 ± 0.027
3.299ThrAsp: 3.299 ± 0.096
3.426ThrGlu: 3.426 ± 0.081
2.801ThrPhe: 2.801 ± 0.061
4.134ThrGly: 4.134 ± 0.095
1.096ThrHis: 1.096 ± 0.046
4.571ThrIle: 4.571 ± 0.094
3.305ThrLys: 3.305 ± 0.095
5.284ThrLeu: 5.284 ± 0.094
1.2ThrMet: 1.2 ± 0.045
2.549ThrAsn: 2.549 ± 0.098
2.279ThrPro: 2.279 ± 0.07
1.768ThrGln: 1.768 ± 0.063
1.959ThrArg: 1.959 ± 0.058
3.531ThrSer: 3.531 ± 0.087
3.156ThrThr: 3.156 ± 0.106
4.37ThrVal: 4.37 ± 0.119
0.523ThrTrp: 0.523 ± 0.034
2.224ThrTyr: 2.224 ± 0.089
0.0ThrXaa: 0.0 ± 0.0
Val
6.027ValAla: 6.027 ± 0.13
0.428ValCys: 0.428 ± 0.029
3.923ValAsp: 3.923 ± 0.089
4.683ValGlu: 4.683 ± 0.1
2.963ValPhe: 2.963 ± 0.085
4.449ValGly: 4.449 ± 0.112
1.128ValHis: 1.128 ± 0.043
4.645ValIle: 4.645 ± 0.095
4.678ValLys: 4.678 ± 0.096
7.002ValLeu: 7.002 ± 0.123
1.573ValMet: 1.573 ± 0.052
2.976ValAsn: 2.976 ± 0.071
2.437ValPro: 2.437 ± 0.073
2.161ValGln: 2.161 ± 0.059
2.802ValArg: 2.802 ± 0.082
4.345ValSer: 4.345 ± 0.086
4.427ValThr: 4.427 ± 0.116
4.927ValVal: 4.927 ± 0.1
0.559ValTrp: 0.559 ± 0.032
2.264ValTyr: 2.264 ± 0.067
0.0ValXaa: 0.0 ± 0.0
Trp
0.558TrpAla: 0.558 ± 0.031
0.08TrpCys: 0.08 ± 0.011
0.507TrpAsp: 0.507 ± 0.032
0.514TrpGlu: 0.514 ± 0.034
0.383TrpPhe: 0.383 ± 0.027
0.558TrpGly: 0.558 ± 0.036
0.187TrpHis: 0.187 ± 0.02
0.52TrpIle: 0.52 ± 0.031
0.617TrpLys: 0.617 ± 0.03
1.062TrpLeu: 1.062 ± 0.051
0.184TrpMet: 0.184 ± 0.02
0.483TrpAsn: 0.483 ± 0.031
0.168TrpPro: 0.168 ± 0.02
0.464TrpGln: 0.464 ± 0.029
0.372TrpArg: 0.372 ± 0.025
0.49TrpSer: 0.49 ± 0.032
0.499TrpThr: 0.499 ± 0.031
0.497TrpVal: 0.497 ± 0.029
0.099TrpTrp: 0.099 ± 0.011
0.341TrpTyr: 0.341 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.638TyrAla: 2.638 ± 0.063
0.208TyrCys: 0.208 ± 0.018
2.371TyrAsp: 2.371 ± 0.07
2.435TyrGlu: 2.435 ± 0.072
1.82TyrPhe: 1.82 ± 0.056
2.435TyrGly: 2.435 ± 0.073
0.961TyrHis: 0.961 ± 0.042
2.354TyrIle: 2.354 ± 0.066
2.262TyrLys: 2.262 ± 0.062
4.074TyrLeu: 4.074 ± 0.089
0.746TyrMet: 0.746 ± 0.036
1.569TyrAsn: 1.569 ± 0.057
1.424TyrPro: 1.424 ± 0.044
2.309TyrGln: 2.309 ± 0.066
1.746TyrArg: 1.746 ± 0.057
2.258TyrSer: 2.258 ± 0.066
1.921TyrThr: 1.921 ± 0.077
2.071TyrVal: 2.071 ± 0.061
0.407TyrTrp: 0.407 ± 0.027
1.578TyrTyr: 1.578 ± 0.052
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1899 proteins (577382 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski