Trypanosoma rangeli
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10070 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A422NHB0|A0A422NHB0_TRYRA Uncharacterized protein OS=Trypanosoma rangeli OX=5698 GN=TraAM80_04960 PE=4 SV=1
MM1 pKa = 7.18 FAVDD5 pKa = 5.44 LLDD8 pKa = 4.35 ALAEE12 pKa = 4.01 AAVILLFDD20 pKa = 4.1 WTPTSACGDD29 pKa = 3.69 EE30 pKa = 5.45 DD31 pKa = 4.34 NDD33 pKa = 4.43 GEE35 pKa = 4.32 IGSEE39 pKa = 3.93 RR40 pKa = 11.84 FTGLNSWSSLDD51 pKa = 3.38 SCFPRR56 pKa = 11.84 SQMNTTLSANNASLTASRR74 pKa = 11.84 TLSLDD79 pKa = 3.16 CSSFLQVAAEE89 pKa = 4.75 EE90 pKa = 4.46 IQTTPSEE97 pKa = 4.17 QLVGSQDD104 pKa = 3.45 HH105 pKa = 5.82 YY106 pKa = 11.38 CWTNEE111 pKa = 3.57 YY112 pKa = 10.3 EE113 pKa = 4.43 YY114 pKa = 10.76 FNSSSSTPSSWAVPVRR130 pKa = 11.84 SLEE133 pKa = 4.04 QKK135 pKa = 10.44
Molecular weight: 14.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.872
IPC_protein 3.795
Toseland 3.605
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.528
Solomon 3.757
Lehninger 3.719
Nozaki 3.91
DTASelect 4.075
Thurlkill 3.656
EMBOSS 3.706
Sillero 3.923
Patrickios 0.846
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A422NW00|A0A422NW00_TRYRA V-type proton ATPase proteolipid subunit OS=Trypanosoma rangeli OX=5698 GN=TraAM80_02012 PE=3 SV=1
MM1 pKa = 7.77 PSTVARR7 pKa = 11.84 EE8 pKa = 3.91 RR9 pKa = 11.84 NKK11 pKa = 10.56 AAVRR15 pKa = 11.84 RR16 pKa = 11.84 LFARR20 pKa = 11.84 DD21 pKa = 3.87 HH22 pKa = 6.42 VPSPAPWKK30 pKa = 10.4 CHH32 pKa = 5.01 TVHH35 pKa = 6.73 CADD38 pKa = 3.42 GTLPPGMHH46 pKa = 7.24 LSKK49 pKa = 10.56 QCEE52 pKa = 3.97 LAHH55 pKa = 5.37 EE56 pKa = 4.61 VGRR59 pKa = 11.84 GTAAAVAVAAAAATSSPHH77 pKa = 5.74 SRR79 pKa = 11.84 AVDD82 pKa = 3.47 YY83 pKa = 10.96 GNPFCSCYY91 pKa = 10.96 SMNRR95 pKa = 11.84 KK96 pKa = 8.53 IARR99 pKa = 11.84 HH100 pKa = 4.77 EE101 pKa = 4.05 WATSRR106 pKa = 11.84 AEE108 pKa = 3.93 KK109 pKa = 10.65 GKK111 pKa = 10.09 LQGGPLNPHH120 pKa = 5.91 KK121 pKa = 10.45 SARR124 pKa = 11.84 EE125 pKa = 3.29 RR126 pKa = 11.84 RR127 pKa = 11.84 AAIEE131 pKa = 3.94 HH132 pKa = 6.52 EE133 pKa = 4.28 EE134 pKa = 4.21 AILRR138 pKa = 11.84 YY139 pKa = 8.86 EE140 pKa = 3.81 LSIPRR145 pKa = 11.84 RR146 pKa = 11.84 RR147 pKa = 11.84 VAGHH151 pKa = 7.44 LLHH154 pKa = 6.78 LTAAQLSTSQPMPRR168 pKa = 11.84 ARR170 pKa = 11.84 TMDD173 pKa = 3.48 RR174 pKa = 11.84 LVEE177 pKa = 3.98 HH178 pKa = 7.24 RR179 pKa = 11.84 KK180 pKa = 10.04 GCWVLHH186 pKa = 5.23 NPYY189 pKa = 9.85 RR190 pKa = 11.84 QQLLRR195 pKa = 11.84 GEE197 pKa = 4.25 PVMTRR202 pKa = 11.84 IMHH205 pKa = 5.98 GEE207 pKa = 3.97 FYY209 pKa = 10.61 EE210 pKa = 4.18 PQRR213 pKa = 11.84 NEE215 pKa = 3.45 QNTINFSQANKK226 pKa = 9.79 RR227 pKa = 11.84 HH228 pKa = 4.85 VQVASSINHH237 pKa = 6.13 AMHH240 pKa = 6.98 SSRR243 pKa = 11.84 NTRR246 pKa = 3.1
Molecular weight: 27.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.358
IPC2_protein 9.56
IPC_protein 10.423
Toseland 10.613
ProMoST 10.511
Dawson 10.716
Bjellqvist 10.467
Wikipedia 10.95
Rodwell 10.847
Grimsley 10.774
Solomon 10.847
Lehninger 10.818
Nozaki 10.628
DTASelect 10.452
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.657
Patrickios 10.555
IPC_peptide 10.847
IPC2_peptide 9.721
IPC2.peptide.svr19 8.654
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10070
0
10070
4520539
93
5642
448.9
49.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.199 ± 0.026
2.138 ± 0.012
4.771 ± 0.015
6.859 ± 0.024
3.636 ± 0.017
6.549 ± 0.02
2.672 ± 0.011
3.6 ± 0.017
4.123 ± 0.021
9.986 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.471 ± 0.009
3.223 ± 0.013
4.995 ± 0.019
3.996 ± 0.016
7.276 ± 0.024
7.526 ± 0.024
5.691 ± 0.017
7.577 ± 0.018
1.225 ± 0.008
2.427 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here