Pseudomonas brassicae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4227 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B3P341|A0A6B3P341_9PSED Amidophosphoribosyltransferase OS=Pseudomonas brassicae OX=2708063 GN=purF PE=3 SV=1
SS1 pKa = 6.87SFLGGALQTAAGVAGGVMLAQGISSLFNHH30 pKa = 6.87HH31 pKa = 6.74SSPPQEE37 pKa = 3.89VTEE40 pKa = 4.86VINEE44 pKa = 4.18APTPAADD51 pKa = 4.05SGWGGNQEE59 pKa = 4.36QRR61 pKa = 11.84LTADD65 pKa = 2.97NGGYY69 pKa = 9.49SQDD72 pKa = 3.24QGGFTDD78 pKa = 2.86THH80 pKa = 6.33YY81 pKa = 11.59ADD83 pKa = 4.85DD84 pKa = 4.57GGGFFADD91 pKa = 4.23DD92 pKa = 3.86DD93 pKa = 4.58AFVV96 pKa = 3.36

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B3NR57|A0A6B3NR57_9PSED DUF2059 domain-containing protein OS=Pseudomonas brassicae OX=2708063 GN=G3435_05040 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4227

0

4227

1204593

21

1934

285.0

31.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.457 ± 0.049

1.052 ± 0.013

5.269 ± 0.027

5.371 ± 0.037

3.562 ± 0.026

7.96 ± 0.038

2.402 ± 0.021

4.477 ± 0.033

3.162 ± 0.032

11.99 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.019

2.903 ± 0.024

4.821 ± 0.029

4.876 ± 0.034

6.586 ± 0.037

5.789 ± 0.034

4.795 ± 0.029

7.244 ± 0.033

1.459 ± 0.017

2.58 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski