Amino acid dipepetide frequency for Pseudomonas brassicae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	13.313AlaAla: 13.313 ± 0.152	1.39AlaCys: 1.39 ± 0.033	5.97AlaAsp: 5.97 ± 0.085	6.484AlaGlu: 6.484 ± 0.091	3.983AlaPhe: 3.983 ± 0.06	9.222AlaGly: 9.222 ± 0.107	2.466AlaHis: 2.466 ± 0.058	5.005AlaIle: 5.005 ± 0.069	3.353AlaLys: 3.353 ± 0.062	14.798AlaLeu: 14.798 ± 0.139	2.882AlaMet: 2.882 ± 0.047	2.969AlaAsn: 2.969 ± 0.056	5.022AlaPro: 5.022 ± 0.068	6.207AlaGln: 6.207 ± 0.102	7.715AlaArg: 7.715 ± 0.1	6.507AlaSer: 6.507 ± 0.079	5.001AlaThr: 5.001 ± 0.072	7.804AlaVal: 7.804 ± 0.092	1.753AlaTrp: 1.753 ± 0.046	2.729AlaTyr: 2.729 ± 0.051	0.0AlaXaa: 0.0 ± 0.0
Cys	1.246CysAla: 1.246 ± 0.038	0.173CysCys: 0.173 ± 0.012	0.534CysAsp: 0.534 ± 0.02	0.568CysGlu: 0.568 ± 0.022	0.319CysPhe: 0.319 ± 0.017	0.971CysGly: 0.971 ± 0.031	0.265CysHis: 0.265 ± 0.015	0.473CysIle: 0.473 ± 0.02	0.276CysLys: 0.276 ± 0.018	1.18CysLeu: 1.18 ± 0.03	0.238CysMet: 0.238 ± 0.015	0.284CysAsn: 0.284 ± 0.015	0.531CysPro: 0.531 ± 0.022	0.449CysGln: 0.449 ± 0.016	0.649CysArg: 0.649 ± 0.025	0.623CysSer: 0.623 ± 0.023	0.55CysThr: 0.55 ± 0.022	0.737CysVal: 0.737 ± 0.027	0.182CysTrp: 0.182 ± 0.012	0.267CysTyr: 0.267 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	6.009AspAla: 6.009 ± 0.079	0.54AspCys: 0.54 ± 0.021	2.907AspAsp: 2.907 ± 0.058	3.206AspGlu: 3.206 ± 0.061	2.052AspPhe: 2.052 ± 0.043	4.431AspGly: 4.431 ± 0.069	1.162AspHis: 1.162 ± 0.032	2.451AspIle: 2.451 ± 0.051	1.777AspLys: 1.777 ± 0.039	5.811AspLeu: 5.811 ± 0.062	1.112AspMet: 1.112 ± 0.033	1.592AspAsn: 1.592 ± 0.048	2.826AspPro: 2.826 ± 0.047	2.178AspGln: 2.178 ± 0.044	2.955AspArg: 2.955 ± 0.051	2.989AspSer: 2.989 ± 0.057	2.564AspThr: 2.564 ± 0.048	3.473AspVal: 3.473 ± 0.054	0.92AspTrp: 0.92 ± 0.031	1.736AspTyr: 1.736 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	6.044GluAla: 6.044 ± 0.076	0.434GluCys: 0.434 ± 0.018	2.277GluAsp: 2.277 ± 0.039	2.583GluGlu: 2.583 ± 0.062	1.693GluPhe: 1.693 ± 0.043	3.817GluGly: 3.817 ± 0.061	1.875GluHis: 1.875 ± 0.042	2.417GluIle: 2.417 ± 0.049	1.751GluLys: 1.751 ± 0.041	6.645GluLeu: 6.645 ± 0.086	1.16GluMet: 1.16 ± 0.033	1.326GluAsn: 1.326 ± 0.034	2.397GluPro: 2.397 ± 0.043	3.975GluGln: 3.975 ± 0.055	4.68GluArg: 4.68 ± 0.068	2.386GluSer: 2.386 ± 0.047	2.227GluThr: 2.227 ± 0.043	4.15GluVal: 4.15 ± 0.076	0.676GluTrp: 0.676 ± 0.024	1.2GluTyr: 1.2 ± 0.036	0.0GluXaa: 0.0 ± 0.0
Phe	4.121PheAla: 4.121 ± 0.067	0.454PheCys: 0.454 ± 0.018	2.462PheAsp: 2.462 ± 0.045	2.153PheGlu: 2.153 ± 0.04	1.353PhePhe: 1.353 ± 0.039	3.162PheGly: 3.162 ± 0.055	0.718PheHis: 0.718 ± 0.027	1.744PheIle: 1.744 ± 0.035	1.323PheLys: 1.323 ± 0.037	3.052PheLeu: 3.052 ± 0.058	0.802PheMet: 0.802 ± 0.027	1.378PheAsn: 1.378 ± 0.035	1.313PhePro: 1.313 ± 0.034	1.18PheGln: 1.18 ± 0.031	1.659PheArg: 1.659 ± 0.043	2.537PheSer: 2.537 ± 0.044	1.897PheThr: 1.897 ± 0.04	2.635PheVal: 2.635 ± 0.056	0.575PheTrp: 0.575 ± 0.023	1.098PheTyr: 1.098 ± 0.037	0.0PheXaa: 0.0 ± 0.0
Gly	7.765GlyAla: 7.765 ± 0.103	1.04GlyCys: 1.04 ± 0.033	3.933GlyAsp: 3.933 ± 0.059	4.545GlyGlu: 4.545 ± 0.055	3.287GlyPhe: 3.287 ± 0.059	5.811GlyGly: 5.811 ± 0.083	1.935GlyHis: 1.935 ± 0.041	3.903GlyIle: 3.903 ± 0.069	3.236GlyLys: 3.236 ± 0.058	9.586GlyLeu: 9.586 ± 0.107	2.171GlyMet: 2.171 ± 0.047	2.406GlyAsn: 2.406 ± 0.055	2.681GlyPro: 2.681 ± 0.046	3.834GlyGln: 3.834 ± 0.059	4.879GlyArg: 4.879 ± 0.069	4.693GlySer: 4.693 ± 0.081	3.781GlyThr: 3.781 ± 0.068	6.256GlyVal: 6.256 ± 0.076	1.378GlyTrp: 1.378 ± 0.038	2.475GlyTyr: 2.475 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.705HisAla: 2.705 ± 0.052	0.381HisCys: 0.381 ± 0.018	1.319HisAsp: 1.319 ± 0.036	1.172HisGlu: 1.172 ± 0.036	0.99HisPhe: 0.99 ± 0.031	2.093HisGly: 2.093 ± 0.046	0.634HisHis: 0.634 ± 0.022	0.963HisIle: 0.963 ± 0.028	0.645HisLys: 0.645 ± 0.02	2.872HisLeu: 2.872 ± 0.047	0.527HisMet: 0.527 ± 0.022	0.687HisAsn: 0.687 ± 0.024	1.386HisPro: 1.386 ± 0.033	1.027HisGln: 1.027 ± 0.029	1.362HisArg: 1.362 ± 0.035	1.385HisSer: 1.385 ± 0.029	1.144HisThr: 1.144 ± 0.033	1.449HisVal: 1.449 ± 0.032	0.481HisTrp: 0.481 ± 0.02	0.798HisTyr: 0.798 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.698IleAla: 5.698 ± 0.089	0.481IleCys: 0.481 ± 0.022	3.089IleAsp: 3.089 ± 0.049	3.184IleGlu: 3.184 ± 0.051	1.31IlePhe: 1.31 ± 0.03	4.223IleGly: 4.223 ± 0.068	0.858IleHis: 0.858 ± 0.027	1.849IleIle: 1.849 ± 0.039	1.669IleLys: 1.669 ± 0.039	3.776IleLeu: 3.776 ± 0.058	0.77IleMet: 0.77 ± 0.025	1.669IleAsn: 1.669 ± 0.043	2.093IlePro: 2.093 ± 0.042	1.525IleGln: 1.525 ± 0.039	2.718IleArg: 2.718 ± 0.05	2.79IleSer: 2.79 ± 0.05	2.447IleThr: 2.447 ± 0.049	3.122IleVal: 3.122 ± 0.058	0.459IleTrp: 0.459 ± 0.022	1.044IleTyr: 1.044 ± 0.031	0.0IleXaa: 0.0 ± 0.0
Lys	3.99LysAla: 3.99 ± 0.062	0.178LysCys: 0.178 ± 0.012	1.687LysAsp: 1.687 ± 0.044	1.385LysGlu: 1.385 ± 0.04	0.789LysPhe: 0.789 ± 0.028	2.578LysGly: 2.578 ± 0.054	0.778LysHis: 0.778 ± 0.026	1.399LysIle: 1.399 ± 0.035	1.156LysLys: 1.156 ± 0.042	3.412LysLeu: 3.412 ± 0.053	0.643LysMet: 0.643 ± 0.025	0.858LysAsn: 0.858 ± 0.028	1.876LysPro: 1.876 ± 0.046	1.567LysGln: 1.567 ± 0.04	2.315LysArg: 2.315 ± 0.049	1.592LysSer: 1.592 ± 0.037	1.632LysThr: 1.632 ± 0.037	2.772LysVal: 2.772 ± 0.051	0.33LysTrp: 0.33 ± 0.018	0.68LysTyr: 0.68 ± 0.023	0.0LysXaa: 0.0 ± 0.0
Leu	14.711LeuAla: 14.711 ± 0.114	1.34LeuCys: 1.34 ± 0.036	6.846LeuAsp: 6.846 ± 0.087	6.423LeuGlu: 6.423 ± 0.074	4.197LeuPhe: 4.197 ± 0.064	9.648LeuGly: 9.648 ± 0.099	2.737LeuHis: 2.737 ± 0.054	5.27LeuIle: 5.27 ± 0.077	4.093LeuLys: 4.093 ± 0.071	13.778LeuLeu: 13.778 ± 0.171	2.573LeuMet: 2.573 ± 0.055	3.668LeuAsn: 3.668 ± 0.058	6.259LeuPro: 6.259 ± 0.078	5.3LeuGln: 5.3 ± 0.08	7.67LeuArg: 7.67 ± 0.092	7.542LeuSer: 7.542 ± 0.081	5.639LeuThr: 5.639 ± 0.062	8.053LeuVal: 8.053 ± 0.104	1.435LeuTrp: 1.435 ± 0.041	2.722LeuTyr: 2.722 ± 0.046	0.0LeuXaa: 0.0 ± 0.0
Met	2.672MetAla: 2.672 ± 0.047	0.175MetCys: 0.175 ± 0.011	0.939MetAsp: 0.939 ± 0.025	0.857MetGlu: 0.857 ± 0.028	0.684MetPhe: 0.684 ± 0.026	1.693MetGly: 1.693 ± 0.04	0.568MetHis: 0.568 ± 0.025	1.089MetIle: 1.089 ± 0.03	0.822MetLys: 0.822 ± 0.023	2.705MetLeu: 2.705 ± 0.053	0.449MetMet: 0.449 ± 0.017	0.843MetAsn: 0.843 ± 0.027	1.392MetPro: 1.392 ± 0.03	1.054MetGln: 1.054 ± 0.029	1.488MetArg: 1.488 ± 0.039	1.68MetSer: 1.68 ± 0.037	1.353MetThr: 1.353 ± 0.035	1.444MetVal: 1.444 ± 0.038	0.174MetTrp: 0.174 ± 0.013	0.375MetTyr: 0.375 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	3.251AsnAla: 3.251 ± 0.055	0.265AsnCys: 0.265 ± 0.015	1.558AsnAsp: 1.558 ± 0.04	1.325AsnGlu: 1.325 ± 0.037	0.98AsnPhe: 0.98 ± 0.027	2.513AsnGly: 2.513 ± 0.057	0.641AsnHis: 0.641 ± 0.021	1.317AsnIle: 1.317 ± 0.036	0.874AsnLys: 0.874 ± 0.028	3.438AsnLeu: 3.438 ± 0.056	0.534AsnMet: 0.534 ± 0.021	0.96AsnAsn: 0.96 ± 0.032	1.899AsnPro: 1.899 ± 0.039	1.278AsnGln: 1.278 ± 0.032	1.868AsnArg: 1.868 ± 0.046	1.557AsnSer: 1.557 ± 0.042	1.548AsnThr: 1.548 ± 0.034	1.939AsnVal: 1.939 ± 0.044	0.46AsnTrp: 0.46 ± 0.02	0.828AsnTyr: 0.828 ± 0.025	0.0AsnXaa: 0.0 ± 0.0
Pro	6.01ProAla: 6.01 ± 0.077	0.434ProCys: 0.434 ± 0.021	2.726ProAsp: 2.726 ± 0.051	2.836ProGlu: 2.836 ± 0.05	1.727ProPhe: 1.727 ± 0.033	4.175ProGly: 4.175 ± 0.071	1.173ProHis: 1.173 ± 0.029	1.838ProIle: 1.838 ± 0.039	1.391ProLys: 1.391 ± 0.042	5.796ProLeu: 5.796 ± 0.068	1.163ProMet: 1.163 ± 0.034	1.268ProAsn: 1.268 ± 0.03	1.861ProPro: 1.861 ± 0.047	2.477ProGln: 2.477 ± 0.045	2.647ProArg: 2.647 ± 0.046	2.665ProSer: 2.665 ± 0.05	2.221ProThr: 2.221 ± 0.043	3.859ProVal: 3.859 ± 0.055	0.762ProTrp: 0.762 ± 0.027	1.182ProTyr: 1.182 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	6.944GlnAla: 6.944 ± 0.096	0.366GlnCys: 0.366 ± 0.019	1.96GlnAsp: 1.96 ± 0.042	1.691GlnGlu: 1.691 ± 0.036	1.375GlnPhe: 1.375 ± 0.034	3.827GlnGly: 3.827 ± 0.059	1.327GlnHis: 1.327 ± 0.037	2.024GlnIle: 2.024 ± 0.037	1.163GlnLys: 1.163 ± 0.032	6.014GlnLeu: 6.014 ± 0.083	1.115GlnMet: 1.115 ± 0.033	0.98GlnAsn: 0.98 ± 0.03	2.563GlnPro: 2.563 ± 0.052	3.122GlnGln: 3.122 ± 0.068	4.043GlnArg: 4.043 ± 0.066	2.265GlnSer: 2.265 ± 0.048	1.992GlnThr: 1.992 ± 0.042	4.123GlnVal: 4.123 ± 0.064	0.8GlnTrp: 0.8 ± 0.027	1.065GlnTyr: 1.065 ± 0.034	0.0GlnXaa: 0.0 ± 0.0
Arg	6.325ArgAla: 6.325 ± 0.085	0.64ArgCys: 0.64 ± 0.023	3.445ArgAsp: 3.445 ± 0.061	4.198ArgGlu: 4.198 ± 0.066	2.666ArgPhe: 2.666 ± 0.046	4.106ArgGly: 4.106 ± 0.061	1.884ArgHis: 1.884 ± 0.045	3.179ArgIle: 3.179 ± 0.049	1.946ArgLys: 1.946 ± 0.048	8.66ArgLeu: 8.66 ± 0.089	1.633ArgMet: 1.633 ± 0.034	1.923ArgAsn: 1.923 ± 0.044	2.777ArgPro: 2.777 ± 0.053	3.655ArgGln: 3.655 ± 0.057	4.441ArgArg: 4.441 ± 0.068	3.698ArgSer: 3.698 ± 0.062	2.892ArgThr: 2.892 ± 0.05	4.614ArgVal: 4.614 ± 0.067	1.089ArgTrp: 1.089 ± 0.033	2.088ArgTyr: 2.088 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.55SerAla: 6.55 ± 0.078	0.467SerCys: 0.467 ± 0.019	2.862SerAsp: 2.862 ± 0.054	2.899SerGlu: 2.899 ± 0.052	2.207SerPhe: 2.207 ± 0.047	5.064SerGly: 5.064 ± 0.079	1.347SerHis: 1.347 ± 0.036	2.717SerIle: 2.717 ± 0.053	1.69SerLys: 1.69 ± 0.039	7.23SerLeu: 7.23 ± 0.096	1.377SerMet: 1.377 ± 0.034	1.745SerAsn: 1.745 ± 0.038	2.676SerPro: 2.676 ± 0.047	2.461SerGln: 2.461 ± 0.046	3.642SerArg: 3.642 ± 0.054	3.469SerSer: 3.469 ± 0.067	3.082SerThr: 3.082 ± 0.051	4.098SerVal: 4.098 ± 0.06	0.798SerTrp: 0.798 ± 0.027	1.504SerTyr: 1.504 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	5.166ThrAla: 5.166 ± 0.065	0.491ThrCys: 0.491 ± 0.022	2.328ThrAsp: 2.328 ± 0.048	2.185ThrGlu: 2.185 ± 0.047	1.794ThrPhe: 1.794 ± 0.041	4.029ThrGly: 4.029 ± 0.056	1.119ThrHis: 1.119 ± 0.029	1.791ThrIle: 1.791 ± 0.042	0.975ThrLys: 0.975 ± 0.026	6.993ThrLeu: 6.993 ± 0.101	0.72ThrMet: 0.72 ± 0.023	1.102ThrAsn: 1.102 ± 0.032	3.177ThrPro: 3.177 ± 0.051	2.07ThrGln: 2.07 ± 0.049	3.289ThrArg: 3.289 ± 0.052	2.654ThrSer: 2.654 ± 0.048	2.428ThrThr: 2.428 ± 0.051	3.619ThrVal: 3.619 ± 0.062	0.732ThrTrp: 0.732 ± 0.025	1.285ThrTyr: 1.285 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	8.104ValAla: 8.104 ± 0.084	0.76ValCys: 0.76 ± 0.028	3.834ValAsp: 3.834 ± 0.049	4.298ValGlu: 4.298 ± 0.07	2.647ValPhe: 2.647 ± 0.052	5.293ValGly: 5.293 ± 0.077	1.527ValHis: 1.527 ± 0.037	3.76ValIle: 3.76 ± 0.062	2.274ValLys: 2.274 ± 0.048	8.654ValLeu: 8.654 ± 0.1	1.715ValMet: 1.715 ± 0.043	2.172ValAsn: 2.172 ± 0.047	3.423ValPro: 3.423 ± 0.055	3.091ValGln: 3.091 ± 0.053	4.577ValArg: 4.577 ± 0.068	4.482ValSer: 4.482 ± 0.057	3.635ValThr: 3.635 ± 0.058	5.592ValVal: 5.592 ± 0.085	0.92ValTrp: 0.92 ± 0.028	1.678ValTyr: 1.678 ± 0.036	0.0ValXaa: 0.0 ± 0.0
Trp	1.26TrpAla: 1.26 ± 0.033	0.184TrpCys: 0.184 ± 0.013	0.612TrpAsp: 0.612 ± 0.025	0.58TrpGlu: 0.58 ± 0.025	0.565TrpPhe: 0.565 ± 0.023	0.935TrpGly: 0.935 ± 0.032	0.422TrpHis: 0.422 ± 0.02	0.624TrpIle: 0.624 ± 0.023	0.419TrpLys: 0.419 ± 0.017	2.293TrpLeu: 2.293 ± 0.046	0.398TrpMet: 0.398 ± 0.021	0.467TrpAsn: 0.467 ± 0.017	0.703TrpPro: 0.703 ± 0.025	0.977TrpGln: 0.977 ± 0.033	1.126TrpArg: 1.126 ± 0.033	0.829TrpSer: 0.829 ± 0.024	0.618TrpThr: 0.618 ± 0.023	1.011TrpVal: 1.011 ± 0.031	0.228TrpTrp: 0.228 ± 0.017	0.335TrpTyr: 0.335 ± 0.018	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.69TyrAla: 2.69 ± 0.044	0.323TyrCys: 0.323 ± 0.016	1.399TyrAsp: 1.399 ± 0.046	1.139TyrGlu: 1.139 ± 0.034	1.001TyrPhe: 1.001 ± 0.03	2.006TyrGly: 2.006 ± 0.037	0.585TyrHis: 0.585 ± 0.025	0.96TyrIle: 0.96 ± 0.031	0.785TyrLys: 0.785 ± 0.029	3.211TyrLeu: 3.211 ± 0.059	0.472TyrMet: 0.472 ± 0.017	0.736TyrAsn: 0.736 ± 0.022	1.356TyrPro: 1.356 ± 0.037	1.332TyrGln: 1.332 ± 0.035	2.138TyrArg: 2.138 ± 0.038	1.543TyrSer: 1.543 ± 0.036	1.293TyrThr: 1.293 ± 0.04	1.685TyrVal: 1.685 ± 0.044	0.436TyrTrp: 0.436 ± 0.019	0.715TyrTyr: 0.715 ± 0.028	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4227 proteins (1204593 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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