Drosophila lebanonensis (Fruit fly) (Scaptodrosophila lebanonensis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura;

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 17832 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6J2U9I6|A0A6J2U9I6_DROLE uncharacterized protein LOC115632138 isoform X1 OS=Drosophila lebanonensis OX=7225 GN=LOC115632138 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.54SIFLLLALLALTAICPANVLQCYY25 pKa = 9.04EE26 pKa = 4.89CIGDD30 pKa = 4.65DD31 pKa = 4.63CDD33 pKa = 5.62DD34 pKa = 4.49LNEE37 pKa = 4.28SLLVEE42 pKa = 4.2CSVDD46 pKa = 3.42NTPSTTEE53 pKa = 3.97SPASGTSSAVTDD65 pKa = 3.67HH66 pKa = 6.66TSVSSSDD73 pKa = 3.89DD74 pKa = 3.54DD75 pKa = 4.54RR76 pKa = 11.84SSTTTPTVTTDD87 pKa = 3.17GSSIGPGSSDD97 pKa = 3.29STSGSTEE104 pKa = 4.0TSSLTSTTDD113 pKa = 3.23DD114 pKa = 3.47SSISQDD120 pKa = 3.28STDD123 pKa = 3.6STEE126 pKa = 4.18SSPLTSTTDD135 pKa = 3.19EE136 pKa = 4.33SSISPGSSDD145 pKa = 3.37STSGSTEE152 pKa = 3.93TSPLTSTTDD161 pKa = 3.39DD162 pKa = 3.51SSISPGSTDD171 pKa = 2.81STTDD175 pKa = 2.93EE176 pKa = 4.45STVGSSSSPSSTEE189 pKa = 3.58SSTGSAEE196 pKa = 4.24SSSTAATTDD205 pKa = 3.05EE206 pKa = 4.51TTNEE210 pKa = 4.25SASSEE215 pKa = 4.03RR216 pKa = 11.84STDD219 pKa = 2.94ITSSPTTTEE228 pKa = 3.32ISSPSSTTVASTDD241 pKa = 3.35LSSTTEE247 pKa = 3.86ASSSRR252 pKa = 11.84STNVAATGSSTATTDD267 pKa = 3.36DD268 pKa = 3.72SSISSASTTTTEE280 pKa = 3.68PTEE283 pKa = 4.37AKK285 pKa = 10.33SSLLARR291 pKa = 11.84ASQQLIRR298 pKa = 11.84NYY300 pKa = 10.56RR301 pKa = 11.84SIEE304 pKa = 4.0RR305 pKa = 11.84YY306 pKa = 9.11GRR308 pKa = 11.84AASEE312 pKa = 4.08YY313 pKa = 10.24RR314 pKa = 11.84SAACYY319 pKa = 7.96TLNVGGVIHH328 pKa = 6.94RR329 pKa = 11.84GCVRR333 pKa = 11.84IPQDD337 pKa = 3.02WTSSVCAAVEE347 pKa = 3.91QQIDD351 pKa = 3.64VSDD354 pKa = 3.72NTDD357 pKa = 3.26TDD359 pKa = 3.68DD360 pKa = 4.23CTYY363 pKa = 11.37CLMDD367 pKa = 4.15RR368 pKa = 11.84CNSSASLRR376 pKa = 11.84VSLMAVLLICTLKK389 pKa = 11.07YY390 pKa = 10.82LFF392 pKa = 4.85

Molecular weight: 40.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6J2UF48|A0A6J2UF48_DROLE DET1 homolog isoform X1 OS=Drosophila lebanonensis OX=7225 GN=LOC115633708 PE=4 SV=1
MM1 pKa = 7.62AAHH4 pKa = 6.95KK5 pKa = 10.42SFRR8 pKa = 11.84IKK10 pKa = 10.51QKK12 pKa = 10.12LAKK15 pKa = 9.77KK16 pKa = 9.79LKK18 pKa = 9.05QNRR21 pKa = 11.84SVPQWVRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.47RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.1WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.89LKK50 pKa = 10.55LL51 pKa = 3.39

Molecular weight: 6.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13342

4490

17832

11910080

31

19754

667.9

74.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.642 ± 0.028

1.824 ± 0.031

5.238 ± 0.019

6.459 ± 0.031

3.335 ± 0.017

5.752 ± 0.022

2.629 ± 0.01

4.995 ± 0.016

5.703 ± 0.027

8.948 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.011

5.081 ± 0.017

5.368 ± 0.03

5.41 ± 0.028

5.413 ± 0.017

8.211 ± 0.028

6.105 ± 0.018

5.748 ± 0.017

0.947 ± 0.007

2.915 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski