Deinococcus maricopensis (strain DSM 21211 / LMG 22137 / NRRL B-23946 / LB-34)
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3262 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E8UC11|E8UC11_DEIML Glycosyl transferase group 1 OS=Deinococcus maricopensis (strain DSM 21211 / LMG 22137 / NRRL B-23946 / LB-34) OX=709986 GN=Deima_2971 PE=4 SV=1
MM1 pKa = 7.41 HH2 pKa = 7.73 PATRR6 pKa = 11.84 TTPALIRR13 pKa = 11.84 TLLTLAALLGLTWAPAHH30 pKa = 6.33 AVTFNLSFAGGVDD43 pKa = 3.41 VTTGSTCATALNSRR57 pKa = 11.84 CRR59 pKa = 11.84 FNSVVVGAGTGPYY72 pKa = 9.79 QRR74 pKa = 11.84 DD75 pKa = 3.56 AIITISKK82 pKa = 7.98 LTNAAINNSSVAAPNSDD99 pKa = 4.16 LDD101 pKa = 3.98 NPAPTLTGTAPAASAQDD118 pKa = 3.83 FFSPTVDD125 pKa = 3.04 VSSAGTGTGWAEE137 pKa = 3.53 FTIDD141 pKa = 4.17 FIARR145 pKa = 11.84 GAAVPAVGAGNAALPGTFHH164 pKa = 5.85 VTSFDD169 pKa = 3.32 TDD171 pKa = 3.07 AANPLRR177 pKa = 11.84 EE178 pKa = 4.07 FVEE181 pKa = 4.75 YY182 pKa = 10.39 VAPATTALSSGTTLTSRR199 pKa = 11.84 AAVDD203 pKa = 3.27 GGVAYY208 pKa = 10.53 QSGTTGVSGISTDD221 pKa = 4.26 PIYY224 pKa = 10.62 KK225 pKa = 10.53 ASATYY230 pKa = 10.17 TNAASVRR237 pKa = 11.84 LVYY240 pKa = 10.18 GANTGGTACSGATCEE255 pKa = 4.67 RR256 pKa = 11.84 LTAFDD261 pKa = 5.35 FYY263 pKa = 11.55 VADD266 pKa = 3.98 SVVLQTDD273 pKa = 3.48 VNGFKK278 pKa = 10.48 SARR281 pKa = 11.84 LTTDD285 pKa = 2.79 ADD287 pKa = 3.98 GNGAITPGDD296 pKa = 3.57 TVTYY300 pKa = 9.0 TVTYY304 pKa = 10.65 VNTGNTASTGFQITDD319 pKa = 3.69 ALPSGVTTTAGAQTVRR335 pKa = 11.84 LNGTVTAAARR345 pKa = 11.84 NTAYY349 pKa = 9.7 TGTGANTTLLAAGQTLPANGTISVDD374 pKa = 2.95 IPVVVGAVTSDD385 pKa = 3.15 NTVLSNQASGSGSGATNVLSDD406 pKa = 3.96 NVDD409 pKa = 2.93 ATTAFPPSVTGATGFAAPPAGSVPQTQTAAVSPTTITVRR448 pKa = 11.84 RR449 pKa = 11.84 QPTLTLNKK457 pKa = 9.3 TIAAPGRR464 pKa = 11.84 VNTNDD469 pKa = 2.96 QFTVQISSGGTTVASATTTGTNTTATTGAVTVASGTTYY507 pKa = 10.55 TLSEE511 pKa = 4.1 ALAAGSGSTLSAYY524 pKa = 10.33 ASTMTCTNATGGSSTTLPGGAGVSFTVTPTFGDD557 pKa = 3.86 DD558 pKa = 3.59 LTCTFTNTGTPVDD571 pKa = 4.15 LAVSQTGPATANLDD585 pKa = 3.43 GTVTYY590 pKa = 7.97 TVRR593 pKa = 11.84 VWNNGSDD600 pKa = 3.64 VTGATFTDD608 pKa = 4.14 TVPAALTNVTWTCAATGSASCGATTSGTGNTISATLGALTTDD650 pKa = 3.42 TGSAATADD658 pKa = 3.77 TNYY661 pKa = 8.86 VTFTITGTATSTGSLTNTATVTAPGGFTEE690 pKa = 5.85 SSTTNNSASTSTTVTATVQCSTLYY714 pKa = 9.53 GTFGTGRR721 pKa = 11.84 EE722 pKa = 4.12 LRR724 pKa = 11.84 SVTEE728 pKa = 4.12 TNTVGSLIGTIPDD741 pKa = 3.21 QGTTASASATLAVYY755 pKa = 10.19 GGRR758 pKa = 11.84 FFAARR763 pKa = 11.84 DD764 pKa = 3.48 SDD766 pKa = 3.59 RR767 pKa = 11.84 TLQVLDD773 pKa = 4.36 PATGTWTQGGAFTAGATAGRR793 pKa = 11.84 LVRR796 pKa = 11.84 MAIGPDD802 pKa = 3.3 GTGYY806 pKa = 11.56 AMDD809 pKa = 4.57 GAGSFYY815 pKa = 10.53 TFSTTAPYY823 pKa = 10.13 PVTSGPRR830 pKa = 11.84 TLTITSAGAPAFGGSGDD847 pKa = 4.54 FIVDD851 pKa = 3.47 SLGRR855 pKa = 11.84 LYY857 pKa = 11.17 LLSTAANSTYY867 pKa = 10.71 VDD869 pKa = 4.34 LYY871 pKa = 11.03 EE872 pKa = 5.15 LFASTSTAQYY882 pKa = 10.45 LGRR885 pKa = 11.84 ITNATVINTVFGGFAATPNGIFGRR909 pKa = 11.84 SGTGRR914 pKa = 11.84 LIQVDD919 pKa = 4.49 LANLTVTQIGTDD931 pKa = 3.62 VTPGSTDD938 pKa = 3.4 LASCTYY944 pKa = 8.44 PTFTRR949 pKa = 11.84 TVTATQTVTKK959 pKa = 10.23 VAGSTGTDD967 pKa = 3.25 VRR969 pKa = 11.84 PGDD972 pKa = 3.65 TLEE975 pKa = 4.02 YY976 pKa = 9.76 TVVIRR981 pKa = 11.84 NSGNIAAGQTTFQEE995 pKa = 4.9 AIPAGTTYY1003 pKa = 10.8 VPGTTTLNGTAVTDD1017 pKa = 3.84 AGGAMPFTTAGSVNTPGQTAGVLSPDD1043 pKa = 3.59 TTPGVLDD1050 pKa = 3.8 RR1051 pKa = 11.84 EE1052 pKa = 4.34 AVVRR1056 pKa = 11.84 FRR1058 pKa = 11.84 VVVGTGTTSVSAQGTTTYY1076 pKa = 11.18 SDD1078 pKa = 3.13 VGTQTTLTDD1087 pKa = 3.88 DD1088 pKa = 4.67 PGTATANDD1096 pKa = 4.2 PNVTPVNQPPVANNDD1111 pKa = 3.63 AASTPAGSPVTFSVTANDD1129 pKa = 3.59 TDD1131 pKa = 4.09 TAPGTVNAATVDD1143 pKa = 4.31 LNPSTAALDD1152 pKa = 3.79 TTFTVPNQGTFTVNTAGQVTFTPVAGFSGIVTRR1185 pKa = 11.84 TYY1187 pKa = 10.13 TVQDD1191 pKa = 3.53 NQGFTSNAATITVTVTPRR1209 pKa = 11.84 AVNDD1213 pKa = 3.87 TASTAPSASVNVPVLNNDD1231 pKa = 2.88 VGTGLQPGSVVFVNAPAGSTVTNGGKK1257 pKa = 7.92 TLTNAQGTYY1266 pKa = 8.47 QVQPDD1271 pKa = 3.9 GSVTFTPALGFTGTTTPVSYY1291 pKa = 9.55 TVTDD1295 pKa = 3.54 AAGTISNPATLTITVSSITAPTATNDD1321 pKa = 3.53 AASTPKK1327 pKa = 9.38 GTSVTLPGATNDD1339 pKa = 3.79 TPGTLPIAPATVDD1352 pKa = 4.74 LDD1354 pKa = 4.05 PGTAGQQTTLSVTGGTFTANANGTVTFAPAAGFAGTATATYY1395 pKa = 9.03 TVADD1399 pKa = 3.76 TAGNRR1404 pKa = 11.84 SNAATLTVTVTNQTPTATAATNATLPASAGPTALTPGLSGTDD1446 pKa = 3.3 PDD1448 pKa = 4.03 GTITTFTVTTLPPAGSGTLACGGTPITTVPTSCAPGQLTFDD1489 pKa = 4.08 PAATFSGNAAFQFTVTDD1506 pKa = 4.13 DD1507 pKa = 3.81 NGATSAPATYY1517 pKa = 8.73 TIPVDD1522 pKa = 4.01 APPVAQNDD1530 pKa = 3.92 VASTNPGVPVTFSVTANDD1548 pKa = 3.59 TDD1550 pKa = 4.13 TAPGTVDD1557 pKa = 3.13 PASVVFVNPPAGSTLSPDD1575 pKa = 3.65 GKK1577 pKa = 8.99 TLTTPGVGTLSANADD1592 pKa = 3.5 GTVTFTPAAGFTSGTLTTGYY1612 pKa = 7.95 TVRR1615 pKa = 11.84 DD1616 pKa = 3.51 NLGQVSAPATITVSVPANTDD1636 pKa = 2.9 VALSLTGPAFASPGQPVTYY1655 pKa = 9.57 TLVVTNGGTNVTGTTVTVPLPAGTTFVSASAGGSVSGGTVTWTLGALAPTEE1706 pKa = 4.26 TRR1708 pKa = 11.84 TLTLTLTAPNATFINANGVTTLTTTGTVSVAQSEE1742 pKa = 4.85 STTANNTDD1750 pKa = 3.1 SSSAQLVYY1758 pKa = 10.84 AQLTKK1763 pKa = 9.96 QVRR1766 pKa = 11.84 NVTRR1770 pKa = 11.84 AGPFGVTATGQPNDD1784 pKa = 3.47 VLEE1787 pKa = 4.12 YY1788 pKa = 11.03 CIDD1791 pKa = 3.52 FRR1793 pKa = 11.84 NAGGAALPNFTVLDD1807 pKa = 4.04 PVPTGSAALPGAYY1820 pKa = 9.46 DD1821 pKa = 3.58 AAAGGAGFGVQLTRR1835 pKa = 11.84 GGTTSYY1841 pKa = 9.53 LTSFADD1847 pKa = 3.8 ADD1849 pKa = 3.89 NGQLDD1854 pKa = 3.78 AAGLNVALGTLAVGEE1869 pKa = 4.19 AGRR1872 pKa = 11.84 TCFQARR1878 pKa = 11.84 IPP1880 pKa = 3.85
Molecular weight: 185.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.933
IPC2_protein 3.973
IPC_protein 4.05
Toseland 3.795
ProMoST 4.228
Dawson 4.05
Bjellqvist 4.202
Wikipedia 4.024
Rodwell 3.859
Grimsley 3.694
Solomon 4.05
Lehninger 4.012
Nozaki 4.151
DTASelect 4.495
Thurlkill 3.859
EMBOSS 4.024
Sillero 4.164
Patrickios 2.804
IPC_peptide 4.05
IPC2_peptide 4.139
IPC2.peptide.svr19 4.076
Protein with the highest isoelectric point:
>tr|E8U6Q2|E8U6Q2_DEIML Uncharacterized protein OS=Deinococcus maricopensis (strain DSM 21211 / LMG 22137 / NRRL B-23946 / LB-34) OX=709986 GN=Deima_1088 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 10.11 QPNVRR10 pKa = 11.84 KK11 pKa = 9.49 RR12 pKa = 11.84 AKK14 pKa = 8.27 THH16 pKa = 5.41 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MKK23 pKa = 9.3 TKK25 pKa = 10.49 AGRR28 pKa = 11.84 NVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.03 GRR39 pKa = 11.84 HH40 pKa = 5.4 RR41 pKa = 11.84 LTVSVV46 pKa = 3.92
Molecular weight: 5.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3262
0
3262
1032088
30
3180
316.4
34.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.399 ± 0.064
0.524 ± 0.01
5.658 ± 0.029
4.913 ± 0.045
3.079 ± 0.023
8.691 ± 0.042
2.553 ± 0.033
3.35 ± 0.037
2.163 ± 0.033
11.485 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.779 ± 0.018
2.559 ± 0.029
5.708 ± 0.032
3.473 ± 0.03
7.78 ± 0.047
4.424 ± 0.03
6.721 ± 0.054
8.091 ± 0.039
1.278 ± 0.019
2.372 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here