Ancylostoma ceylanicum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Strongylida; Ancylostomatoidea; Ancylostomatidae; Ancylostomatinae; Ancylostoma

Average proteome isoelectric point is 7.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65375 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A016VAG5|A0A016VAG5_9BILA Isoform of A0A016VB49 EGF-like domain-containing protein OS=Ancylostoma ceylanicum OX=53326 GN=Acey_s0013.g2023 PE=4 SV=1
MM1 pKa = 7.48FSYY4 pKa = 10.34LYY6 pKa = 10.29KK7 pKa = 10.63VQLHH11 pKa = 5.64LHH13 pKa = 5.36TRR15 pKa = 11.84SFYY18 pKa = 10.47IGFPEE23 pKa = 3.77NTILFDD29 pKa = 4.25FSTPAFEE36 pKa = 4.79EE37 pKa = 4.57STEE40 pKa = 4.19GTTPTEE46 pKa = 4.34SMTSLYY52 pKa = 7.98TTMMHH57 pKa = 6.82EE58 pKa = 4.41SSTMSSLEE66 pKa = 4.08TTTMSVTDD74 pKa = 4.51GEE76 pKa = 4.67SSPSLISSTTDD87 pKa = 2.9GATSIQDD94 pKa = 3.67LSTSSGTEE102 pKa = 3.46EE103 pKa = 3.88STATDD108 pKa = 4.01GSSTSVMVSSIEE120 pKa = 3.93TTPAMVSSMEE130 pKa = 4.27TTPFTQSEE138 pKa = 4.45QPSTLEE144 pKa = 4.17VGSEE148 pKa = 4.18TPTVEE153 pKa = 4.36ISSTSHH159 pKa = 6.51DD160 pKa = 3.74TFPYY164 pKa = 9.87PDD166 pKa = 3.92STTQSASTLEE176 pKa = 4.36EE177 pKa = 4.03DD178 pKa = 3.66TSPFDD183 pKa = 3.36VSTTVGVPGSTAAMATTEE201 pKa = 4.01EE202 pKa = 4.68SMGPTAEE209 pKa = 4.14EE210 pKa = 3.71QTMTSAQSTDD220 pKa = 3.06STPNEE225 pKa = 4.23DD226 pKa = 3.51TSSSNTPEE234 pKa = 4.16FTSSSSVSFASSTQSSDD251 pKa = 2.97TDD253 pKa = 3.76GVTSTVSMTTSYY265 pKa = 11.7DD266 pKa = 3.92DD267 pKa = 3.5IVSEE271 pKa = 4.55TTTSSGGIEE280 pKa = 4.3VTTDD284 pKa = 3.07PSTIEE289 pKa = 4.07SSGSTEE295 pKa = 3.68NAAGSTEE302 pKa = 3.76ASSEE306 pKa = 3.88YY307 pKa = 10.58TEE309 pKa = 4.75FVTGSSVTVAQTTPDD324 pKa = 3.39STSIPSEE331 pKa = 4.26SPEE334 pKa = 4.02STTSSEE340 pKa = 4.0QSGLSSSISYY350 pKa = 7.67EE351 pKa = 4.2TTSEE355 pKa = 3.96AVTSSGHH362 pKa = 4.7TDD364 pKa = 3.31EE365 pKa = 5.63PGSTSSMGTTEE376 pKa = 4.58NVPSTDD382 pKa = 3.67GASSSSYY389 pKa = 10.11ISSSEE394 pKa = 3.95LTSSIASEE402 pKa = 4.12ATDD405 pKa = 4.04DD406 pKa = 4.2GAITEE411 pKa = 4.5STISPVVTSSVSGEE425 pKa = 4.25TTVGEE430 pKa = 4.14LTSAGVDD437 pKa = 3.71LSTSPSSDD445 pKa = 2.8GVSEE449 pKa = 4.39SSSSLSEE456 pKa = 4.08STTDD460 pKa = 3.44MAVTSSNPEE469 pKa = 3.79QPTDD473 pKa = 3.47TSITTVEE480 pKa = 4.12RR481 pKa = 11.84TSDD484 pKa = 3.44GVTASTDD491 pKa = 3.49VAVTSYY497 pKa = 11.4SEE499 pKa = 4.33GTSEE503 pKa = 4.16VPTVTGSGTPAEE515 pKa = 4.63STSSDD520 pKa = 3.48PTSAAVTSSGYY531 pKa = 9.75SEE533 pKa = 5.08EE534 pKa = 4.29PTTGHH539 pKa = 5.84STTVDD544 pKa = 3.2EE545 pKa = 5.09SGSTTAFFVTTGSDD559 pKa = 3.04LGASTTEE566 pKa = 3.7SSTAVMTSSEE576 pKa = 4.33FPEE579 pKa = 4.46VSTVPEE585 pKa = 3.89EE586 pKa = 4.35TITDD590 pKa = 4.44SITSEE595 pKa = 4.21SLPSEE600 pKa = 3.95NSVSQMSTTISHH612 pKa = 6.79TSSSAVSEE620 pKa = 4.21YY621 pKa = 10.62TDD623 pKa = 5.17LISTEE628 pKa = 3.95ISQDD632 pKa = 3.32TTTLPPWNIPSSTTDD647 pKa = 3.41DD648 pKa = 4.02TPTTTASWTSTTTISPTTTTSRR670 pKa = 11.84IPDD673 pKa = 4.45IITTTTTTAMPTVSSTLPRR692 pKa = 11.84TSTTTTSPTSTTTKK706 pKa = 10.24IPDD709 pKa = 4.23TITTTTATATTTTTTAMPTVPSTPPRR735 pKa = 11.84TSTTTTSPTSTTTKK749 pKa = 10.22IPDD752 pKa = 3.06ITATTTRR759 pKa = 11.84ATTTTTTTTITTTTAVPTISSTLLSTATVPTTTTVPTVTTGPSVCVDD806 pKa = 3.83GYY808 pKa = 11.29RR809 pKa = 11.84LHH811 pKa = 6.13QANAVVSSAGPGKK824 pKa = 10.46FGGFFTYY831 pKa = 8.73ITSCAEE837 pKa = 3.64LCEE840 pKa = 4.05RR841 pKa = 11.84DD842 pKa = 3.74YY843 pKa = 11.91GLSKK847 pKa = 10.92CLGFAYY853 pKa = 10.09EE854 pKa = 3.83PTNRR858 pKa = 11.84GKK860 pKa = 9.15CTLYY864 pKa = 10.19QRR866 pKa = 11.84SIGAIKK872 pKa = 9.52TDD874 pKa = 3.35SGSTASIYY882 pKa = 10.2KK883 pKa = 10.05RR884 pKa = 11.84CC885 pKa = 3.65

Molecular weight: 90.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A016VEV8|A0A016VEV8_9BILA Uncharacterized protein OS=Ancylostoma ceylanicum OX=53326 GN=Acey_s0011.g1590 PE=3 SV=1
MM1 pKa = 7.07HH2 pKa = 6.52QQFIALRR9 pKa = 11.84RR10 pKa = 11.84SLPVPLFSIIFLVLVIVAVILLISIIPALIGCLTMKK46 pKa = 10.28SKK48 pKa = 10.79RR49 pKa = 11.84NKK51 pKa = 8.9KK52 pKa = 8.81RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84SGKK58 pKa = 9.21HH59 pKa = 4.97SSSTMSGKK67 pKa = 9.91SSKK70 pKa = 10.41RR71 pKa = 11.84SGKK74 pKa = 10.62GKK76 pKa = 10.33GGPNRR81 pKa = 11.84KK82 pKa = 9.41RR83 pKa = 11.84GGKK86 pKa = 8.43SKK88 pKa = 10.6KK89 pKa = 10.03SSTSTTSTKK98 pKa = 10.18TGTSTRR104 pKa = 11.84SGKK107 pKa = 10.33KK108 pKa = 9.33SASKK112 pKa = 10.6SKK114 pKa = 10.58SSQLSEE120 pKa = 3.87KK121 pKa = 9.85SARR124 pKa = 11.84GAGRR128 pKa = 11.84NGRR131 pKa = 11.84PANKK135 pKa = 9.82GMKK138 pKa = 9.62SKK140 pKa = 10.75SKK142 pKa = 10.65SKK144 pKa = 10.66SKK146 pKa = 10.78SAGKK150 pKa = 9.41SGQMRR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84KK158 pKa = 9.51SRR160 pKa = 11.84RR161 pKa = 3.25

Molecular weight: 17.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

36519

28856

65375

20039791

17

8404

306.5

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.207 ± 0.012

2.343 ± 0.011

5.199 ± 0.009

6.332 ± 0.016

4.103 ± 0.008

5.657 ± 0.013

2.467 ± 0.005

5.236 ± 0.01

5.556 ± 0.014

8.896 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.547 ± 0.006

4.08 ± 0.008

5.285 ± 0.013

3.803 ± 0.009

6.531 ± 0.013

8.016 ± 0.014

5.868 ± 0.015

6.641 ± 0.009

1.218 ± 0.004

3.004 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski