Paracoccus aminovorans
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3736 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1I3FJQ1|A0A1I3FJQ1_9RHOB PPC domain-containing protein OS=Paracoccus aminovorans OX=34004 GN=SAMN04488021_1794 PE=4 SV=1
MM1 pKa = 7.51 ANAVGRR7 pKa = 11.84 TFTAIGFLLEE17 pKa = 4.07 SPEE20 pKa = 4.02 IVEE23 pKa = 4.8 ALHH26 pKa = 5.84 TRR28 pKa = 11.84 QGLAGVTTQFAGAIAGVAGGLWGASIGAAAAASMAAALVGLLAGMPITILSAVAVVGISFIAASISEE95 pKa = 5.01 AIVEE99 pKa = 4.37 SLVTQLFEE107 pKa = 5.42 GMGDD111 pKa = 3.59 DD112 pKa = 5.66 LKK114 pKa = 11.7 VHH116 pKa = 6.19 TNDD119 pKa = 2.43 VDD121 pKa = 4.0 VYY123 pKa = 10.3 IGGLRR128 pKa = 11.84 GEE130 pKa = 4.31 TLMAGSADD138 pKa = 3.33 DD139 pKa = 3.75 FVYY142 pKa = 10.83 GRR144 pKa = 11.84 GGNDD148 pKa = 3.34 NISGGTGSDD157 pKa = 3.31 HH158 pKa = 7.33 LLGGSGNDD166 pKa = 3.78 TITGGSGDD174 pKa = 4.25 DD175 pKa = 4.28 FISGGSGSNNMTGGVGNDD193 pKa = 3.19 SYY195 pKa = 11.71 VVEE198 pKa = 4.51 SEE200 pKa = 3.78 GDD202 pKa = 3.72 TVNEE206 pKa = 4.04 NVNGGVDD213 pKa = 3.6 KK214 pKa = 10.84 IVANISINLNDD225 pKa = 3.23 ARR227 pKa = 11.84 YY228 pKa = 10.19 ANVEE232 pKa = 3.9 NVTLIGEE239 pKa = 4.51 SNISATGSDD248 pKa = 4.18 HH249 pKa = 7.51 DD250 pKa = 4.5 NSLIGYY256 pKa = 8.51 LGNNRR261 pKa = 11.84 LYY263 pKa = 11.38 GNDD266 pKa = 3.72 GDD268 pKa = 4.53 DD269 pKa = 4.43 HH270 pKa = 8.24 LSGSYY275 pKa = 11.32 GNDD278 pKa = 2.88 TLYY281 pKa = 11.3 GGGGNDD287 pKa = 3.5 TLNGGFGLDD296 pKa = 3.78 SMIGGAGNDD305 pKa = 3.08 IYY307 pKa = 11.62 YY308 pKa = 10.4 VDD310 pKa = 4.69 DD311 pKa = 4.96 LGDD314 pKa = 3.68 VVIEE318 pKa = 4.15 GSNGGEE324 pKa = 3.9 DD325 pKa = 4.16 TINSAISINLDD336 pKa = 3.46 RR337 pKa = 11.84 PNDD340 pKa = 3.27 AYY342 pKa = 11.0 ANVEE346 pKa = 4.14 HH347 pKa = 6.84 VVLQGTEE354 pKa = 3.98 NINAYY359 pKa = 10.33 GSVSDD364 pKa = 5.05 NSLTGNRR371 pKa = 11.84 GNNLLNGRR379 pKa = 11.84 AGNDD383 pKa = 3.85 SIVGAAGNDD392 pKa = 3.64 TLYY395 pKa = 11.45 GEE397 pKa = 4.97 AGNDD401 pKa = 3.3 ILNGGAGVDD410 pKa = 3.78 SMVGGTGNDD419 pKa = 2.82 IYY421 pKa = 11.02 FVDD424 pKa = 5.55 DD425 pKa = 3.2 IRR427 pKa = 11.84 DD428 pKa = 3.92 IIVEE432 pKa = 4.25 TATGGTDD439 pKa = 3.97 RR440 pKa = 11.84 INSSISIDD448 pKa = 3.64 LNRR451 pKa = 11.84 SGDD454 pKa = 3.46 VYY456 pKa = 11.72 ANIEE460 pKa = 4.16 NIILLGAQNLNAYY473 pKa = 9.9 GSGGSNNITGNSGANIISGRR493 pKa = 11.84 YY494 pKa = 9.56 GNDD497 pKa = 2.78 NLAGGAGADD506 pKa = 3.2 TFIFSKK512 pKa = 10.9 NYY514 pKa = 9.5 DD515 pKa = 3.25 QDD517 pKa = 4.0 VITDD521 pKa = 4.31 FEE523 pKa = 5.95 DD524 pKa = 3.35 NTDD527 pKa = 4.06 TIRR530 pKa = 11.84 LLNFGVTTFTQAQGYY545 pKa = 8.45 AADD548 pKa = 3.6 WCKK551 pKa = 10.9 CGII554 pKa = 4.05
Molecular weight: 56.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.668
IPC_protein 3.719
Toseland 3.478
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.389
Solomon 3.719
Lehninger 3.681
Nozaki 3.834
DTASelect 4.113
Thurlkill 3.541
EMBOSS 3.681
Sillero 3.846
Patrickios 1.087
IPC_peptide 3.706
IPC2_peptide 3.821
IPC2.peptide.svr19 3.761
Protein with the highest isoelectric point:
>tr|A0A1I3BQP5|A0A1I3BQP5_9RHOB Two-component system OmpR family response regulator OS=Paracoccus aminovorans OX=34004 GN=SAMN04488021_1245 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 ARR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.37 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.12 GGRR29 pKa = 11.84 RR30 pKa = 11.84 VLNARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.03 GRR40 pKa = 11.84 KK41 pKa = 6.51 TLSAA45 pKa = 4.15
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3736
0
3736
1156815
39
2363
309.6
33.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.476 ± 0.068
0.836 ± 0.01
5.795 ± 0.029
5.628 ± 0.034
3.453 ± 0.024
9.048 ± 0.038
2.033 ± 0.018
4.788 ± 0.029
2.636 ± 0.035
10.449 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.608 ± 0.018
2.293 ± 0.029
5.65 ± 0.031
3.269 ± 0.023
7.789 ± 0.047
4.781 ± 0.027
4.966 ± 0.027
6.993 ± 0.03
1.493 ± 0.017
2.016 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here