Cyanophage S-SSM6b

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 218 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M1UGC3|M1UGC3_9CAUD Uncharacterized protein OS=Cyanophage S-SSM6b OX=682651 GN=CYWG_00053 PE=4 SV=1
MM1 pKa = 7.77TDD3 pKa = 3.73FFDD6 pKa = 5.88NEE8 pKa = 4.31TSDD11 pKa = 3.64QQHH14 pKa = 6.83SIDD17 pKa = 4.32NMGDD21 pKa = 3.46SLIEE25 pKa = 3.89AMRR28 pKa = 11.84VAIGQGRR35 pKa = 11.84IEE37 pKa = 4.44DD38 pKa = 3.72AASISDD44 pKa = 3.37EE45 pKa = 4.67WITPDD50 pKa = 5.63CNDD53 pKa = 3.67PCNGEE58 pKa = 4.02YY59 pKa = 10.79EE60 pKa = 5.12FIFLKK65 pKa = 11.04NNTFGDD71 pKa = 4.21WTWDD75 pKa = 3.12ACASS79 pKa = 3.6

Molecular weight: 8.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M1U9W8|M1U9W8_9CAUD Uncharacterized protein OS=Cyanophage S-SSM6b OX=682651 GN=CYWG_00057 PE=4 SV=1
MM1 pKa = 7.87AKK3 pKa = 10.14NQMKK7 pKa = 10.11QMKK10 pKa = 10.2KK11 pKa = 9.88LMQRR15 pKa = 11.84NGFEE19 pKa = 4.19LTRR22 pKa = 11.84EE23 pKa = 4.19TKK25 pKa = 10.49HH26 pKa = 6.56FIWKK30 pKa = 9.47HH31 pKa = 3.45KK32 pKa = 8.65TGVVMTTSKK41 pKa = 9.89TPSDD45 pKa = 3.79NYY47 pKa = 11.18AIAQASRR54 pKa = 11.84QIRR57 pKa = 11.84RR58 pKa = 11.84TIGKK62 pKa = 8.92VVV64 pKa = 2.82

Molecular weight: 7.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

218

0

218

57731

43

6932

264.8

29.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.816 ± 0.152

0.875 ± 0.081

6.904 ± 0.103

6.184 ± 0.263

4.356 ± 0.087

7.682 ± 0.244

1.585 ± 0.122

6.208 ± 0.216

6.232 ± 0.433

7.123 ± 0.175

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.108 ± 0.204

5.976 ± 0.189

4.14 ± 0.144

3.845 ± 0.076

3.871 ± 0.138

6.775 ± 0.169

7.232 ± 0.342

6.632 ± 0.204

1.225 ± 0.089

4.23 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski