Amino acid dipepetide frequency for Cyanophage S-SSM6b

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.855AlaAla: 5.855 ± 0.493
0.468AlaCys: 0.468 ± 0.119
4.036AlaAsp: 4.036 ± 0.294
4.036AlaGlu: 4.036 ± 0.29
2.685AlaPhe: 2.685 ± 0.214
6.461AlaGly: 6.461 ± 0.584
0.883AlaHis: 0.883 ± 0.145
3.794AlaIle: 3.794 ± 0.256
4.053AlaLys: 4.053 ± 0.403
4.66AlaLeu: 4.66 ± 0.32
1.542AlaMet: 1.542 ± 0.234
3.759AlaAsn: 3.759 ± 0.334
2.772AlaPro: 2.772 ± 0.229
2.789AlaGln: 2.789 ± 0.224
2.598AlaArg: 2.598 ± 0.214
4.954AlaSer: 4.954 ± 0.285
5.734AlaThr: 5.734 ± 0.651
4.036AlaVal: 4.036 ± 0.324
0.762AlaTrp: 0.762 ± 0.126
2.287AlaTyr: 2.287 ± 0.232
0.0AlaXaa: 0.0 ± 0.0
Cys
0.45CysAla: 0.45 ± 0.118
0.069CysCys: 0.069 ± 0.045
0.797CysAsp: 0.797 ± 0.126
0.606CysGlu: 0.606 ± 0.13
0.398CysPhe: 0.398 ± 0.081
0.554CysGly: 0.554 ± 0.11
0.225CysHis: 0.225 ± 0.069
0.416CysIle: 0.416 ± 0.085
0.485CysLys: 0.485 ± 0.094
0.502CysLeu: 0.502 ± 0.103
0.243CysMet: 0.243 ± 0.074
0.416CysAsn: 0.416 ± 0.097
0.346CysPro: 0.346 ± 0.094
0.294CysGln: 0.294 ± 0.066
0.416CysArg: 0.416 ± 0.089
0.658CysSer: 0.658 ± 0.121
0.745CysThr: 0.745 ± 0.111
0.52CysVal: 0.52 ± 0.095
0.173CysTrp: 0.173 ± 0.056
0.433CysTyr: 0.433 ± 0.091
0.0CysXaa: 0.0 ± 0.0
Asp
4.66AspAla: 4.66 ± 0.296
0.693AspCys: 0.693 ± 0.109
4.833AspAsp: 4.833 ± 0.32
4.261AspGlu: 4.261 ± 0.408
3.135AspPhe: 3.135 ± 0.254
5.959AspGly: 5.959 ± 0.374
1.091AspHis: 1.091 ± 0.16
4.261AspIle: 4.261 ± 0.292
3.69AspLys: 3.69 ± 0.328
5.422AspLeu: 5.422 ± 0.295
1.438AspMet: 1.438 ± 0.179
3.845AspAsn: 3.845 ± 0.297
3.863AspPro: 3.863 ± 0.313
2.408AspGln: 2.408 ± 0.17
2.668AspArg: 2.668 ± 0.248
3.949AspSer: 3.949 ± 0.254
4.157AspThr: 4.157 ± 0.299
4.486AspVal: 4.486 ± 0.336
0.953AspTrp: 0.953 ± 0.11
3.274AspTyr: 3.274 ± 0.245
0.0AspXaa: 0.0 ± 0.0
Glu
3.62GluAla: 3.62 ± 0.236
0.537GluCys: 0.537 ± 0.11
4.123GluAsp: 4.123 ± 0.325
4.781GluGlu: 4.781 ± 0.614
3.257GluPhe: 3.257 ± 0.282
4.14GluGly: 4.14 ± 0.307
1.005GluHis: 1.005 ± 0.2
4.4GluIle: 4.4 ± 0.301
3.967GluLys: 3.967 ± 0.53
5.093GluLeu: 5.093 ± 0.408
1.559GluMet: 1.559 ± 0.244
3.291GluAsn: 3.291 ± 0.222
1.923GluPro: 1.923 ± 0.182
2.39GluGln: 2.39 ± 0.231
2.373GluArg: 2.373 ± 0.206
3.845GluSer: 3.845 ± 0.341
3.343GluThr: 3.343 ± 0.241
4.365GluVal: 4.365 ± 0.296
0.866GluTrp: 0.866 ± 0.182
2.962GluTyr: 2.962 ± 0.237
0.0GluXaa: 0.0 ± 0.0
Phe
2.564PheAla: 2.564 ± 0.195
0.416PheCys: 0.416 ± 0.093
3.672PheAsp: 3.672 ± 0.252
2.789PheGlu: 2.789 ± 0.205
1.559PhePhe: 1.559 ± 0.204
2.962PheGly: 2.962 ± 0.295
0.485PheHis: 0.485 ± 0.133
3.205PheIle: 3.205 ± 0.217
2.442PheLys: 2.442 ± 0.231
2.685PheLeu: 2.685 ± 0.256
1.005PheMet: 1.005 ± 0.196
3.031PheAsn: 3.031 ± 0.244
1.698PhePro: 1.698 ± 0.192
1.853PheGln: 1.853 ± 0.165
1.611PheArg: 1.611 ± 0.131
3.447PheSer: 3.447 ± 0.235
3.343PheThr: 3.343 ± 0.3
2.737PheVal: 2.737 ± 0.191
0.45PheTrp: 0.45 ± 0.101
1.611PheTyr: 1.611 ± 0.157
0.0PheXaa: 0.0 ± 0.0
Gly
5.526GlyAla: 5.526 ± 0.516
0.658GlyCys: 0.658 ± 0.102
5.526GlyAsp: 5.526 ± 0.497
4.105GlyGlu: 4.105 ± 0.223
3.205GlyPhe: 3.205 ± 0.278
7.379GlyGly: 7.379 ± 0.592
1.23GlyHis: 1.23 ± 0.164
4.608GlyIle: 4.608 ± 0.348
4.331GlyLys: 4.331 ± 0.417
4.625GlyLeu: 4.625 ± 0.344
1.663GlyMet: 1.663 ± 0.249
4.434GlyAsn: 4.434 ± 0.538
2.113GlyPro: 2.113 ± 0.237
2.945GlyGln: 2.945 ± 0.219
2.772GlyArg: 2.772 ± 0.228
5.56GlySer: 5.56 ± 0.716
6.86GlyThr: 6.86 ± 0.686
4.694GlyVal: 4.694 ± 0.276
1.109GlyTrp: 1.109 ± 0.148
3.482GlyTyr: 3.482 ± 0.298
0.0GlyXaa: 0.0 ± 0.0
His
0.849HisAla: 0.849 ± 0.105
0.208HisCys: 0.208 ± 0.068
1.265HisAsp: 1.265 ± 0.18
0.935HisGlu: 0.935 ± 0.161
0.779HisPhe: 0.779 ± 0.138
1.022HisGly: 1.022 ± 0.16
0.468HisHis: 0.468 ± 0.151
0.935HisIle: 0.935 ± 0.139
1.022HisLys: 1.022 ± 0.192
0.987HisLeu: 0.987 ± 0.169
0.329HisMet: 0.329 ± 0.082
0.814HisAsn: 0.814 ± 0.16
0.935HisPro: 0.935 ± 0.143
0.468HisGln: 0.468 ± 0.083
0.641HisArg: 0.641 ± 0.122
1.005HisSer: 1.005 ± 0.152
1.057HisThr: 1.057 ± 0.137
0.935HisVal: 0.935 ± 0.138
0.26HisTrp: 0.26 ± 0.084
0.935HisTyr: 0.935 ± 0.151
0.0HisXaa: 0.0 ± 0.0
Ile
4.175IleAla: 4.175 ± 0.256
0.502IleCys: 0.502 ± 0.088
5.023IleAsp: 5.023 ± 0.335
4.227IleGlu: 4.227 ± 0.355
2.165IlePhe: 2.165 ± 0.196
3.949IleGly: 3.949 ± 0.362
0.641IleHis: 0.641 ± 0.116
3.378IleIle: 3.378 ± 0.317
4.105IleLys: 4.105 ± 0.326
4.209IleLeu: 4.209 ± 0.272
1.143IleMet: 1.143 ± 0.173
4.071IleAsn: 4.071 ± 0.289
3.257IlePro: 3.257 ± 0.266
2.668IleGln: 2.668 ± 0.226
2.287IleArg: 2.287 ± 0.271
4.313IleSer: 4.313 ± 0.476
4.989IleThr: 4.989 ± 0.634
4.279IleVal: 4.279 ± 0.318
0.537IleTrp: 0.537 ± 0.097
2.165IleTyr: 2.165 ± 0.18
0.0IleXaa: 0.0 ± 0.0
Lys
3.984LysAla: 3.984 ± 0.473
0.52LysCys: 0.52 ± 0.101
3.776LysAsp: 3.776 ± 0.409
4.227LysGlu: 4.227 ± 0.572
3.153LysPhe: 3.153 ± 0.231
3.568LysGly: 3.568 ± 0.426
1.23LysHis: 1.23 ± 0.205
3.759LysIle: 3.759 ± 0.35
5.734LysLys: 5.734 ± 1.005
4.677LysLeu: 4.677 ± 0.416
1.819LysMet: 1.819 ± 0.3
3.17LysAsn: 3.17 ± 0.309
2.321LysPro: 2.321 ± 0.298
2.235LysGln: 2.235 ± 0.33
2.65LysArg: 2.65 ± 0.325
3.69LysSer: 3.69 ± 0.342
3.88LysThr: 3.88 ± 0.335
4.192LysVal: 4.192 ± 0.327
0.935LysTrp: 0.935 ± 0.126
2.806LysTyr: 2.806 ± 0.297
0.0LysXaa: 0.0 ± 0.0
Leu
4.712LeuAla: 4.712 ± 0.266
0.572LeuCys: 0.572 ± 0.134
5.578LeuAsp: 5.578 ± 0.319
4.452LeuGlu: 4.452 ± 0.321
2.997LeuPhe: 2.997 ± 0.213
4.556LeuGly: 4.556 ± 0.274
1.351LeuHis: 1.351 ± 0.189
3.759LeuIle: 3.759 ± 0.218
4.85LeuLys: 4.85 ± 0.527
5.127LeuLeu: 5.127 ± 0.313
1.109LeuMet: 1.109 ± 0.163
4.625LeuAsn: 4.625 ± 0.272
2.997LeuPro: 2.997 ± 0.345
3.101LeuGln: 3.101 ± 0.249
3.118LeuArg: 3.118 ± 0.235
4.902LeuSer: 4.902 ± 0.314
5.699LeuThr: 5.699 ± 0.429
4.123LeuVal: 4.123 ± 0.261
0.728LeuTrp: 0.728 ± 0.094
2.858LeuTyr: 2.858 ± 0.279
0.0LeuXaa: 0.0 ± 0.0
Met
1.68MetAla: 1.68 ± 0.31
0.191MetCys: 0.191 ± 0.057
1.386MetAsp: 1.386 ± 0.185
1.178MetGlu: 1.178 ± 0.2
0.866MetPhe: 0.866 ± 0.152
1.195MetGly: 1.195 ± 0.206
0.381MetHis: 0.381 ± 0.117
1.074MetIle: 1.074 ± 0.146
2.148MetLys: 2.148 ± 0.356
1.594MetLeu: 1.594 ± 0.187
0.502MetMet: 0.502 ± 0.104
1.126MetAsn: 1.126 ± 0.165
0.849MetPro: 0.849 ± 0.155
0.883MetGln: 0.883 ± 0.154
0.797MetArg: 0.797 ± 0.117
1.594MetSer: 1.594 ± 0.26
1.628MetThr: 1.628 ± 0.199
1.091MetVal: 1.091 ± 0.194
0.26MetTrp: 0.26 ± 0.069
0.658MetTyr: 0.658 ± 0.109
0.0MetXaa: 0.0 ± 0.0
Asn
4.521AsnAla: 4.521 ± 0.347
0.537AsnCys: 0.537 ± 0.101
3.031AsnAsp: 3.031 ± 0.26
3.43AsnGlu: 3.43 ± 0.258
2.823AsnPhe: 2.823 ± 0.211
4.885AsnGly: 4.885 ± 0.49
0.849AsnHis: 0.849 ± 0.115
3.759AsnIle: 3.759 ± 0.387
3.274AsnLys: 3.274 ± 0.354
4.919AsnLeu: 4.919 ± 0.417
1.057AsnMet: 1.057 ± 0.154
3.655AsnAsn: 3.655 ± 0.376
3.239AsnPro: 3.239 ± 0.218
2.044AsnGln: 2.044 ± 0.204
2.061AsnArg: 2.061 ± 0.187
3.88AsnSer: 3.88 ± 0.349
4.538AsnThr: 4.538 ± 0.378
3.932AsnVal: 3.932 ± 0.449
0.918AsnTrp: 0.918 ± 0.145
2.408AsnTyr: 2.408 ± 0.195
0.0AsnXaa: 0.0 ± 0.0
Pro
2.823ProAla: 2.823 ± 0.265
0.485ProCys: 0.485 ± 0.149
3.274ProAsp: 3.274 ± 0.276
2.927ProGlu: 2.927 ± 0.269
1.905ProPhe: 1.905 ± 0.176
3.187ProGly: 3.187 ± 0.27
0.97ProHis: 0.97 ± 0.134
2.46ProIle: 2.46 ± 0.244
2.096ProLys: 2.096 ± 0.277
2.373ProLeu: 2.373 ± 0.239
0.745ProMet: 0.745 ± 0.159
2.408ProAsn: 2.408 ± 0.216
1.836ProPro: 1.836 ± 0.261
1.282ProGln: 1.282 ± 0.183
1.559ProArg: 1.559 ± 0.167
3.135ProSer: 3.135 ± 0.203
3.014ProThr: 3.014 ± 0.204
2.737ProVal: 2.737 ± 0.235
0.52ProTrp: 0.52 ± 0.105
1.663ProTyr: 1.663 ± 0.159
0.0ProXaa: 0.0 ± 0.0
Gln
2.494GlnAla: 2.494 ± 0.22
0.277GlnCys: 0.277 ± 0.068
2.131GlnAsp: 2.131 ± 0.211
2.287GlnGlu: 2.287 ± 0.213
1.836GlnPhe: 1.836 ± 0.174
2.806GlnGly: 2.806 ± 0.226
0.693GlnHis: 0.693 ± 0.131
2.529GlnIle: 2.529 ± 0.185
2.772GlnLys: 2.772 ± 0.332
3.153GlnLeu: 3.153 ± 0.212
0.987GlnMet: 0.987 ± 0.147
2.096GlnAsn: 2.096 ± 0.196
1.334GlnPro: 1.334 ± 0.138
1.507GlnGln: 1.507 ± 0.169
1.715GlnArg: 1.715 ± 0.159
2.494GlnSer: 2.494 ± 0.234
2.39GlnThr: 2.39 ± 0.214
2.373GlnVal: 2.373 ± 0.206
0.624GlnTrp: 0.624 ± 0.119
1.957GlnTyr: 1.957 ± 0.2
0.0GlnXaa: 0.0 ± 0.0
Arg
2.408ArgAla: 2.408 ± 0.222
0.312ArgCys: 0.312 ± 0.074
2.737ArgAsp: 2.737 ± 0.246
2.252ArgGlu: 2.252 ± 0.274
1.975ArgPhe: 1.975 ± 0.173
2.789ArgGly: 2.789 ± 0.251
0.676ArgHis: 0.676 ± 0.13
2.789ArgIle: 2.789 ± 0.214
2.442ArgLys: 2.442 ± 0.303
2.893ArgLeu: 2.893 ± 0.309
0.797ArgMet: 0.797 ± 0.152
1.871ArgAsn: 1.871 ± 0.21
1.403ArgPro: 1.403 ± 0.157
1.784ArgGln: 1.784 ± 0.198
1.576ArgArg: 1.576 ± 0.219
2.321ArgSer: 2.321 ± 0.181
2.113ArgThr: 2.113 ± 0.225
3.222ArgVal: 3.222 ± 0.263
0.398ArgTrp: 0.398 ± 0.099
1.957ArgTyr: 1.957 ± 0.204
0.0ArgXaa: 0.0 ± 0.0
Ser
4.937SerAla: 4.937 ± 0.39
0.554SerCys: 0.554 ± 0.088
3.915SerAsp: 3.915 ± 0.271
3.69SerGlu: 3.69 ± 0.197
3.014SerPhe: 3.014 ± 0.181
6.686SerGly: 6.686 ± 0.73
0.866SerHis: 0.866 ± 0.113
4.331SerIle: 4.331 ± 0.336
3.534SerLys: 3.534 ± 0.37
4.556SerLeu: 4.556 ± 0.231
1.611SerMet: 1.611 ± 0.23
4.279SerAsn: 4.279 ± 0.368
2.442SerPro: 2.442 ± 0.236
2.477SerGln: 2.477 ± 0.197
2.616SerArg: 2.616 ± 0.179
5.578SerSer: 5.578 ± 0.54
4.486SerThr: 4.486 ± 0.388
4.608SerVal: 4.608 ± 0.291
0.762SerTrp: 0.762 ± 0.144
2.806SerTyr: 2.806 ± 0.188
0.0SerXaa: 0.0 ± 0.0
Thr
5.249ThrAla: 5.249 ± 0.509
0.537ThrCys: 0.537 ± 0.106
4.452ThrAsp: 4.452 ± 0.373
4.036ThrGlu: 4.036 ± 0.259
3.257ThrPhe: 3.257 ± 0.355
6.53ThrGly: 6.53 ± 0.561
0.97ThrHis: 0.97 ± 0.122
5.526ThrIle: 5.526 ± 0.644
3.638ThrLys: 3.638 ± 0.33
5.526ThrLeu: 5.526 ± 0.386
1.195ThrMet: 1.195 ± 0.183
4.521ThrAsn: 4.521 ± 0.481
2.927ThrPro: 2.927 ± 0.283
2.442ThrGln: 2.442 ± 0.227
2.304ThrArg: 2.304 ± 0.198
4.573ThrSer: 4.573 ± 0.437
5.335ThrThr: 5.335 ± 0.663
5.612ThrVal: 5.612 ± 0.696
0.779ThrTrp: 0.779 ± 0.119
2.91ThrTyr: 2.91 ± 0.203
0.0ThrXaa: 0.0 ± 0.0
Val
4.573ValAla: 4.573 ± 0.3
0.433ValCys: 0.433 ± 0.106
5.266ValAsp: 5.266 ± 0.459
4.192ValGlu: 4.192 ± 0.37
2.581ValPhe: 2.581 ± 0.234
4.816ValGly: 4.816 ± 0.5
0.953ValHis: 0.953 ± 0.163
4.053ValIle: 4.053 ± 0.398
3.949ValLys: 3.949 ± 0.339
4.348ValLeu: 4.348 ± 0.293
1.178ValMet: 1.178 ± 0.168
4.365ValAsn: 4.365 ± 0.439
2.668ValPro: 2.668 ± 0.256
2.529ValGln: 2.529 ± 0.191
2.442ValArg: 2.442 ± 0.176
4.573ValSer: 4.573 ± 0.344
5.422ValThr: 5.422 ± 0.505
4.556ValVal: 4.556 ± 0.336
0.71ValTrp: 0.71 ± 0.112
2.72ValTyr: 2.72 ± 0.204
0.0ValXaa: 0.0 ± 0.0
Trp
0.728TrpAla: 0.728 ± 0.104
0.191TrpCys: 0.191 ± 0.055
0.918TrpAsp: 0.918 ± 0.118
0.693TrpGlu: 0.693 ± 0.125
0.433TrpPhe: 0.433 ± 0.095
0.814TrpGly: 0.814 ± 0.114
0.225TrpHis: 0.225 ± 0.06
0.762TrpIle: 0.762 ± 0.12
0.953TrpLys: 0.953 ± 0.152
0.728TrpLeu: 0.728 ± 0.113
0.364TrpMet: 0.364 ± 0.074
0.849TrpAsn: 0.849 ± 0.135
0.364TrpPro: 0.364 ± 0.077
0.606TrpGln: 0.606 ± 0.092
0.658TrpArg: 0.658 ± 0.13
0.883TrpSer: 0.883 ± 0.139
0.658TrpThr: 0.658 ± 0.097
0.901TrpVal: 0.901 ± 0.122
0.156TrpTrp: 0.156 ± 0.054
0.364TrpTyr: 0.364 ± 0.087
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.356TyrAla: 2.356 ± 0.195
0.589TyrCys: 0.589 ± 0.096
3.309TyrAsp: 3.309 ± 0.259
2.737TyrGlu: 2.737 ± 0.302
1.542TyrPhe: 1.542 ± 0.147
2.564TyrGly: 2.564 ± 0.176
0.658TyrHis: 0.658 ± 0.123
2.287TyrIle: 2.287 ± 0.254
2.841TyrLys: 2.841 ± 0.275
3.153TyrLeu: 3.153 ± 0.254
0.779TyrMet: 0.779 ± 0.104
3.135TyrAsn: 3.135 ± 0.212
2.113TyrPro: 2.113 ± 0.188
1.75TyrGln: 1.75 ± 0.197
1.853TyrArg: 1.853 ± 0.212
2.39TyrSer: 2.39 ± 0.172
2.927TyrThr: 2.927 ± 0.359
2.927TyrVal: 2.927 ± 0.195
0.346TyrTrp: 0.346 ± 0.097
2.044TyrTyr: 2.044 ± 0.215
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 218 proteins (57731 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski