Duncaniella freteri

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; Duncaniella

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2847 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Z0V5W0|A0A4Z0V5W0_9BACT YtxH domain-containing protein OS=Duncaniella freteri OX=2530391 GN=EZ315_08030 PE=4 SV=1
MM1 pKa = 7.03QLSPNSLAFVALGNEE16 pKa = 3.83YY17 pKa = 10.49CAAIEE22 pKa = 4.23NASQSDD28 pKa = 3.75PQAFIAEE35 pKa = 4.47MVRR38 pKa = 11.84LLPRR42 pKa = 11.84IYY44 pKa = 9.48ITASDD49 pKa = 4.64LKK51 pKa = 10.05PAPSLDD57 pKa = 3.75EE58 pKa = 4.31EE59 pKa = 4.45PPYY62 pKa = 10.34IDD64 pKa = 4.53SYY66 pKa = 11.14LDD68 pKa = 3.42EE69 pKa = 4.2YY70 pKa = 10.91HH71 pKa = 6.46YY72 pKa = 11.28EE73 pKa = 4.13SLRR76 pKa = 11.84SAIEE80 pKa = 3.98GMLGQDD86 pKa = 3.26DD87 pKa = 4.85VYY89 pKa = 11.65LEE91 pKa = 4.13VFEE94 pKa = 5.74EE95 pKa = 4.05DD96 pKa = 3.32MKK98 pKa = 11.72YY99 pKa = 10.89SDD101 pKa = 3.51TPIRR105 pKa = 11.84ASISEE110 pKa = 4.24GLCDD114 pKa = 4.27IFQVLYY120 pKa = 10.9NFVSSVRR127 pKa = 11.84DD128 pKa = 3.31AAEE131 pKa = 3.89SQINDD136 pKa = 3.31ALIAVSEE143 pKa = 4.43DD144 pKa = 3.62FASFWSQKK152 pKa = 8.72VVNLMRR158 pKa = 11.84PLNHH162 pKa = 6.17IRR164 pKa = 11.84YY165 pKa = 9.73NSDD168 pKa = 2.81EE169 pKa = 4.83DD170 pKa = 5.4DD171 pKa = 4.85DD172 pKa = 4.2MFF174 pKa = 6.79

Molecular weight: 19.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Z0V2Z1|A0A4Z0V2Z1_9BACT Uncharacterized protein OS=Duncaniella freteri OX=2530391 GN=EZ315_13230 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 8.8RR12 pKa = 11.84VNKK15 pKa = 10.01HH16 pKa = 3.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPDD26 pKa = 3.23GRR28 pKa = 11.84KK29 pKa = 9.05VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.35GRR39 pKa = 11.84LRR41 pKa = 11.84LTVSSSIAGKK51 pKa = 10.24

Molecular weight: 5.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2847

0

2847

977413

31

2786

343.3

38.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.8 ± 0.055

1.259 ± 0.017

6.128 ± 0.032

6.142 ± 0.043

4.164 ± 0.028

6.995 ± 0.042

1.971 ± 0.021

6.934 ± 0.038

5.498 ± 0.038

8.617 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.877 ± 0.021

4.77 ± 0.036

4.111 ± 0.027

2.895 ± 0.027

5.541 ± 0.033

6.743 ± 0.041

5.643 ± 0.03

6.627 ± 0.032

1.244 ± 0.019

4.04 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski