Amino acid dipepetide frequency for Duncaniella freteri

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.636AlaAla: 6.636 ± 0.119
0.924AlaCys: 0.924 ± 0.033
5.166AlaAsp: 5.166 ± 0.077
5.097AlaGlu: 5.097 ± 0.088
2.906AlaPhe: 2.906 ± 0.053
5.527AlaGly: 5.527 ± 0.081
1.329AlaHis: 1.329 ± 0.04
5.386AlaIle: 5.386 ± 0.09
4.131AlaLys: 4.131 ± 0.087
7.139AlaLeu: 7.139 ± 0.091
2.379AlaMet: 2.379 ± 0.055
3.135AlaAsn: 3.135 ± 0.055
2.874AlaPro: 2.874 ± 0.059
2.553AlaGln: 2.553 ± 0.061
3.991AlaArg: 3.991 ± 0.066
5.151AlaSer: 5.151 ± 0.081
4.349AlaThr: 4.349 ± 0.071
5.614AlaVal: 5.614 ± 0.096
0.924AlaTrp: 0.924 ± 0.031
2.786AlaTyr: 2.786 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
0.934CysAla: 0.934 ± 0.037
0.166CysCys: 0.166 ± 0.014
0.813CysAsp: 0.813 ± 0.03
0.725CysGlu: 0.725 ± 0.027
0.531CysPhe: 0.531 ± 0.028
1.131CysGly: 1.131 ± 0.038
0.355CysHis: 0.355 ± 0.019
0.808CysIle: 0.808 ± 0.029
0.534CysLys: 0.534 ± 0.029
0.957CysLeu: 0.957 ± 0.029
0.31CysMet: 0.31 ± 0.019
0.631CysAsn: 0.631 ± 0.022
0.607CysPro: 0.607 ± 0.028
0.329CysGln: 0.329 ± 0.018
0.798CysArg: 0.798 ± 0.031
0.879CysSer: 0.879 ± 0.03
0.589CysThr: 0.589 ± 0.026
0.871CysVal: 0.871 ± 0.032
0.132CysTrp: 0.132 ± 0.011
0.489CysTyr: 0.489 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
4.722AspAla: 4.722 ± 0.072
0.796AspCys: 0.796 ± 0.028
3.281AspAsp: 3.281 ± 0.067
3.953AspGlu: 3.953 ± 0.065
2.952AspPhe: 2.952 ± 0.056
4.762AspGly: 4.762 ± 0.084
0.958AspHis: 0.958 ± 0.032
4.79AspIle: 4.79 ± 0.07
3.338AspLys: 3.338 ± 0.07
4.59AspLeu: 4.59 ± 0.078
1.935AspMet: 1.935 ± 0.046
3.085AspAsn: 3.085 ± 0.062
2.418AspPro: 2.418 ± 0.058
1.172AspGln: 1.172 ± 0.042
3.454AspArg: 3.454 ± 0.064
4.019AspSer: 4.019 ± 0.062
3.35AspThr: 3.35 ± 0.061
3.903AspVal: 3.903 ± 0.07
0.808AspTrp: 0.808 ± 0.029
2.992AspTyr: 2.992 ± 0.054
0.0AspXaa: 0.0 ± 0.0
Glu
4.983GluAla: 4.983 ± 0.088
0.724GluCys: 0.724 ± 0.028
3.1GluAsp: 3.1 ± 0.061
4.082GluGlu: 4.082 ± 0.083
2.483GluPhe: 2.483 ± 0.055
3.824GluGly: 3.824 ± 0.072
1.206GluHis: 1.206 ± 0.03
4.618GluIle: 4.618 ± 0.073
4.058GluLys: 4.058 ± 0.076
5.494GluLeu: 5.494 ± 0.08
2.01GluMet: 2.01 ± 0.042
3.041GluAsn: 3.041 ± 0.054
1.966GluPro: 1.966 ± 0.049
2.245GluGln: 2.245 ± 0.064
3.671GluArg: 3.671 ± 0.073
3.534GluSer: 3.534 ± 0.06
3.117GluThr: 3.117 ± 0.064
3.917GluVal: 3.917 ± 0.065
0.874GluTrp: 0.874 ± 0.031
2.475GluTyr: 2.475 ± 0.059
0.0GluXaa: 0.0 ± 0.0
Phe
2.955PheAla: 2.955 ± 0.065
0.58PheCys: 0.58 ± 0.025
2.901PheAsp: 2.901 ± 0.058
2.132PheGlu: 2.132 ± 0.052
1.827PhePhe: 1.827 ± 0.049
3.134PheGly: 3.134 ± 0.062
0.844PheHis: 0.844 ± 0.03
2.906PheIle: 2.906 ± 0.066
2.016PheLys: 2.016 ± 0.048
3.25PheLeu: 3.25 ± 0.064
1.237PheMet: 1.237 ± 0.037
2.395PheAsn: 2.395 ± 0.052
1.745PhePro: 1.745 ± 0.04
0.966PheGln: 0.966 ± 0.029
2.316PheArg: 2.316 ± 0.049
3.215PheSer: 3.215 ± 0.07
2.611PheThr: 2.611 ± 0.05
2.499PheVal: 2.499 ± 0.059
0.501PheTrp: 0.501 ± 0.026
1.611PheTyr: 1.611 ± 0.04
0.0PheXaa: 0.0 ± 0.0
Gly
5.035GlyAla: 5.035 ± 0.079
1.13GlyCys: 1.13 ± 0.04
4.103GlyAsp: 4.103 ± 0.069
4.162GlyGlu: 4.162 ± 0.06
3.092GlyPhe: 3.092 ± 0.053
4.834GlyGly: 4.834 ± 0.091
1.421GlyHis: 1.421 ± 0.047
5.223GlyIle: 5.223 ± 0.077
4.546GlyLys: 4.546 ± 0.082
5.652GlyLeu: 5.652 ± 0.082
2.225GlyMet: 2.225 ± 0.055
3.541GlyAsn: 3.541 ± 0.059
1.526GlyPro: 1.526 ± 0.049
1.883GlyGln: 1.883 ± 0.044
3.635GlyArg: 3.635 ± 0.073
4.498GlySer: 4.498 ± 0.086
3.998GlyThr: 3.998 ± 0.063
5.201GlyVal: 5.201 ± 0.085
1.021GlyTrp: 1.021 ± 0.035
3.224GlyTyr: 3.224 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
1.236HisAla: 1.236 ± 0.036
0.292HisCys: 0.292 ± 0.017
1.189HisAsp: 1.189 ± 0.041
1.054HisGlu: 1.054 ± 0.037
0.884HisPhe: 0.884 ± 0.028
1.435HisGly: 1.435 ± 0.045
0.528HisHis: 0.528 ± 0.024
1.591HisIle: 1.591 ± 0.044
0.964HisLys: 0.964 ± 0.036
1.696HisLeu: 1.696 ± 0.043
0.416HisMet: 0.416 ± 0.019
1.093HisAsn: 1.093 ± 0.033
1.133HisPro: 1.133 ± 0.034
0.478HisGln: 0.478 ± 0.021
1.195HisArg: 1.195 ± 0.033
1.266HisSer: 1.266 ± 0.033
1.189HisThr: 1.189 ± 0.039
1.044HisVal: 1.044 ± 0.032
0.222HisTrp: 0.222 ± 0.013
0.81HisTyr: 0.81 ± 0.032
0.0HisXaa: 0.0 ± 0.0
Ile
6.028IleAla: 6.028 ± 0.092
0.928IleCys: 0.928 ± 0.035
5.048IleAsp: 5.048 ± 0.084
4.459IleGlu: 4.459 ± 0.072
2.688IlePhe: 2.688 ± 0.059
4.738IleGly: 4.738 ± 0.085
1.251IleHis: 1.251 ± 0.038
4.9IleIle: 4.9 ± 0.087
3.767IleLys: 3.767 ± 0.069
5.484IleLeu: 5.484 ± 0.088
1.61IleMet: 1.61 ± 0.045
3.285IleAsn: 3.285 ± 0.06
3.251IlePro: 3.251 ± 0.061
1.708IleGln: 1.708 ± 0.044
3.495IleArg: 3.495 ± 0.068
5.191IleSer: 5.191 ± 0.065
3.881IleThr: 3.881 ± 0.065
4.511IleVal: 4.511 ± 0.072
0.624IleTrp: 0.624 ± 0.024
2.493IleTyr: 2.493 ± 0.053
0.0IleXaa: 0.0 ± 0.0
Lys
4.529LysAla: 4.529 ± 0.072
0.57LysCys: 0.57 ± 0.026
3.373LysAsp: 3.373 ± 0.065
4.005LysGlu: 4.005 ± 0.078
1.994LysPhe: 1.994 ± 0.048
3.913LysGly: 3.913 ± 0.063
1.043LysHis: 1.043 ± 0.032
3.642LysIle: 3.642 ± 0.067
3.469LysLys: 3.469 ± 0.067
4.244LysLeu: 4.244 ± 0.067
1.786LysMet: 1.786 ± 0.049
2.518LysAsn: 2.518 ± 0.06
2.134LysPro: 2.134 ± 0.054
1.674LysGln: 1.674 ± 0.044
3.041LysArg: 3.041 ± 0.062
3.383LysSer: 3.383 ± 0.065
2.843LysThr: 2.843 ± 0.053
3.698LysVal: 3.698 ± 0.063
0.789LysTrp: 0.789 ± 0.028
2.327LysTyr: 2.327 ± 0.052
0.0LysXaa: 0.0 ± 0.0
Leu
6.646LeuAla: 6.646 ± 0.09
1.2LeuCys: 1.2 ± 0.035
5.127LeuAsp: 5.127 ± 0.075
4.541LeuGlu: 4.541 ± 0.075
3.492LeuPhe: 3.492 ± 0.065
5.713LeuGly: 5.713 ± 0.081
1.744LeuHis: 1.744 ± 0.042
5.34LeuIle: 5.34 ± 0.087
4.898LeuLys: 4.898 ± 0.074
7.447LeuLeu: 7.447 ± 0.108
2.483LeuMet: 2.483 ± 0.057
4.233LeuAsn: 4.233 ± 0.068
4.112LeuPro: 4.112 ± 0.065
2.484LeuGln: 2.484 ± 0.063
5.165LeuArg: 5.165 ± 0.076
6.948LeuSer: 6.948 ± 0.09
5.422LeuThr: 5.422 ± 0.076
4.895LeuVal: 4.895 ± 0.086
1.03LeuTrp: 1.03 ± 0.034
3.248LeuTyr: 3.248 ± 0.049
0.0LeuXaa: 0.0 ± 0.0
Met
2.487MetAla: 2.487 ± 0.044
0.307MetCys: 0.307 ± 0.018
1.504MetAsp: 1.504 ± 0.044
1.697MetGlu: 1.697 ± 0.039
1.048MetPhe: 1.048 ± 0.039
1.887MetGly: 1.887 ± 0.049
0.507MetHis: 0.507 ± 0.022
1.626MetIle: 1.626 ± 0.04
1.998MetLys: 1.998 ± 0.044
2.735MetLeu: 2.735 ± 0.06
0.875MetMet: 0.875 ± 0.034
1.342MetAsn: 1.342 ± 0.034
1.361MetPro: 1.361 ± 0.037
0.889MetGln: 0.889 ± 0.028
1.835MetArg: 1.835 ± 0.043
2.0MetSer: 2.0 ± 0.047
1.751MetThr: 1.751 ± 0.047
1.789MetVal: 1.789 ± 0.044
0.328MetTrp: 0.328 ± 0.018
0.805MetTyr: 0.805 ± 0.03
0.0MetXaa: 0.0 ± 0.0
Asn
3.624AsnAla: 3.624 ± 0.064
0.513AsnCys: 0.513 ± 0.026
2.807AsnAsp: 2.807 ± 0.061
2.674AsnGlu: 2.674 ± 0.056
1.924AsnPhe: 1.924 ± 0.046
3.817AsnGly: 3.817 ± 0.082
1.016AsnHis: 1.016 ± 0.037
3.633AsnIle: 3.633 ± 0.072
2.352AsnLys: 2.352 ± 0.058
3.906AsnLeu: 3.906 ± 0.075
1.178AsnMet: 1.178 ± 0.033
2.421AsnAsn: 2.421 ± 0.055
2.733AsnPro: 2.733 ± 0.057
1.277AsnGln: 1.277 ± 0.032
2.648AsnArg: 2.648 ± 0.06
2.87AsnSer: 2.87 ± 0.055
2.561AsnThr: 2.561 ± 0.051
3.182AsnVal: 3.182 ± 0.054
0.573AsnTrp: 0.573 ± 0.024
1.996AsnTyr: 1.996 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
3.309ProAla: 3.309 ± 0.069
0.415ProCys: 0.415 ± 0.02
3.177ProAsp: 3.177 ± 0.063
3.596ProGlu: 3.596 ± 0.063
1.71ProPhe: 1.71 ± 0.045
3.032ProGly: 3.032 ± 0.061
0.834ProHis: 0.834 ± 0.03
2.328ProIle: 2.328 ± 0.054
2.01ProLys: 2.01 ± 0.044
3.415ProLeu: 3.415 ± 0.057
1.155ProMet: 1.155 ± 0.036
1.5ProAsn: 1.5 ± 0.037
1.017ProPro: 1.017 ± 0.036
1.366ProGln: 1.366 ± 0.042
1.863ProArg: 1.863 ± 0.049
2.876ProSer: 2.876 ± 0.056
2.142ProThr: 2.142 ± 0.042
3.207ProVal: 3.207 ± 0.056
0.493ProTrp: 0.493 ± 0.023
1.664ProTyr: 1.664 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.27GlnAla: 2.27 ± 0.063
0.33GlnCys: 0.33 ± 0.018
1.378GlnAsp: 1.378 ± 0.043
1.738GlnGlu: 1.738 ± 0.046
1.256GlnPhe: 1.256 ± 0.038
1.857GlnGly: 1.857 ± 0.052
0.506GlnHis: 0.506 ± 0.023
1.93GlnIle: 1.93 ± 0.047
1.663GlnLys: 1.663 ± 0.045
2.851GlnLeu: 2.851 ± 0.061
0.918GlnMet: 0.918 ± 0.032
1.292GlnAsn: 1.292 ± 0.04
1.19GlnPro: 1.19 ± 0.039
1.221GlnGln: 1.221 ± 0.04
1.734GlnArg: 1.734 ± 0.036
1.872GlnSer: 1.872 ± 0.046
1.561GlnThr: 1.561 ± 0.044
1.787GlnVal: 1.787 ± 0.045
0.425GlnTrp: 0.425 ± 0.021
1.169GlnTyr: 1.169 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
3.64ArgAla: 3.64 ± 0.069
0.647ArgCys: 0.647 ± 0.024
2.993ArgAsp: 2.993 ± 0.059
3.522ArgGlu: 3.522 ± 0.072
2.531ArgPhe: 2.531 ± 0.044
3.189ArgGly: 3.189 ± 0.064
1.368ArgHis: 1.368 ± 0.036
4.028ArgIle: 4.028 ± 0.067
3.206ArgLys: 3.206 ± 0.06
5.61ArgLeu: 5.61 ± 0.098
1.746ArgMet: 1.746 ± 0.04
2.786ArgAsn: 2.786 ± 0.053
2.102ArgPro: 2.102 ± 0.055
2.035ArgGln: 2.035 ± 0.047
3.514ArgArg: 3.514 ± 0.075
3.093ArgSer: 3.093 ± 0.063
2.701ArgThr: 2.701 ± 0.053
3.326ArgVal: 3.326 ± 0.053
0.793ArgTrp: 0.793 ± 0.029
2.584ArgTyr: 2.584 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
5.109SerAla: 5.109 ± 0.083
0.854SerCys: 0.854 ± 0.029
4.221SerAsp: 4.221 ± 0.064
3.878SerGlu: 3.878 ± 0.067
2.989SerPhe: 2.989 ± 0.059
5.047SerGly: 5.047 ± 0.088
1.447SerHis: 1.447 ± 0.041
4.64SerIle: 4.64 ± 0.071
3.138SerLys: 3.138 ± 0.065
6.285SerLeu: 6.285 ± 0.087
1.792SerMet: 1.792 ± 0.041
2.682SerAsn: 2.682 ± 0.055
2.878SerPro: 2.878 ± 0.054
2.033SerGln: 2.033 ± 0.049
3.867SerArg: 3.867 ± 0.078
4.539SerSer: 4.539 ± 0.084
3.751SerThr: 3.751 ± 0.073
4.769SerVal: 4.769 ± 0.076
0.817SerTrp: 0.817 ± 0.032
2.695SerTyr: 2.695 ± 0.062
0.0SerXaa: 0.0 ± 0.0
Thr
4.612ThrAla: 4.612 ± 0.074
0.565ThrCys: 0.565 ± 0.03
3.591ThrAsp: 3.591 ± 0.068
3.112ThrGlu: 3.112 ± 0.063
2.469ThrPhe: 2.469 ± 0.047
4.293ThrGly: 4.293 ± 0.078
1.094ThrHis: 1.094 ± 0.033
3.983ThrIle: 3.983 ± 0.069
2.337ThrLys: 2.337 ± 0.047
5.402ThrLeu: 5.402 ± 0.087
1.339ThrMet: 1.339 ± 0.034
2.175ThrAsn: 2.175 ± 0.054
3.303ThrPro: 3.303 ± 0.059
1.548ThrGln: 1.548 ± 0.044
2.603ThrArg: 2.603 ± 0.052
3.641ThrSer: 3.641 ± 0.066
3.185ThrThr: 3.185 ± 0.07
4.345ThrVal: 4.345 ± 0.071
0.615ThrTrp: 0.615 ± 0.028
2.221ThrTyr: 2.221 ± 0.05
0.0ThrXaa: 0.0 ± 0.0
Val
5.42ValAla: 5.42 ± 0.091
0.848ValCys: 0.848 ± 0.031
4.069ValAsp: 4.069 ± 0.072
4.023ValGlu: 4.023 ± 0.071
2.633ValPhe: 2.633 ± 0.055
4.067ValGly: 4.067 ± 0.077
1.12ValHis: 1.12 ± 0.039
4.553ValIle: 4.553 ± 0.068
3.986ValLys: 3.986 ± 0.064
5.355ValLeu: 5.355 ± 0.077
1.874ValMet: 1.874 ± 0.051
3.478ValAsn: 3.478 ± 0.056
2.747ValPro: 2.747 ± 0.055
1.528ValGln: 1.528 ± 0.038
3.526ValArg: 3.526 ± 0.07
4.862ValSer: 4.862 ± 0.082
4.363ValThr: 4.363 ± 0.066
4.586ValVal: 4.586 ± 0.085
0.726ValTrp: 0.726 ± 0.028
2.511ValTyr: 2.511 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
0.84TrpAla: 0.84 ± 0.029
0.206TrpCys: 0.206 ± 0.018
0.781TrpAsp: 0.781 ± 0.031
0.739TrpGlu: 0.739 ± 0.028
0.452TrpPhe: 0.452 ± 0.023
0.919TrpGly: 0.919 ± 0.033
0.32TrpHis: 0.32 ± 0.02
0.821TrpIle: 0.821 ± 0.03
0.699TrpLys: 0.699 ± 0.029
1.209TrpLeu: 1.209 ± 0.038
0.414TrpMet: 0.414 ± 0.021
0.671TrpAsn: 0.671 ± 0.03
0.23TrpPro: 0.23 ± 0.015
0.469TrpGln: 0.469 ± 0.022
0.702TrpArg: 0.702 ± 0.028
0.799TrpSer: 0.799 ± 0.035
0.69TrpThr: 0.69 ± 0.027
0.759TrpVal: 0.759 ± 0.031
0.227TrpTrp: 0.227 ± 0.015
0.494TrpTyr: 0.494 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.98TyrAla: 2.98 ± 0.056
0.595TyrCys: 0.595 ± 0.024
2.655TyrAsp: 2.655 ± 0.058
2.233TyrGlu: 2.233 ± 0.05
1.782TyrPhe: 1.782 ± 0.044
2.83TyrGly: 2.83 ± 0.061
0.822TyrHis: 0.822 ± 0.03
2.597TyrIle: 2.597 ± 0.051
1.866TyrLys: 1.866 ± 0.043
3.446TyrLeu: 3.446 ± 0.06
1.087TyrMet: 1.087 ± 0.033
2.402TyrAsn: 2.402 ± 0.057
1.782TyrPro: 1.782 ± 0.045
1.095TyrGln: 1.095 ± 0.039
2.361TyrArg: 2.361 ± 0.055
2.795TyrSer: 2.795 ± 0.06
2.379TyrThr: 2.379 ± 0.055
2.371TyrVal: 2.371 ± 0.047
0.517TyrTrp: 0.517 ± 0.026
1.806TyrTyr: 1.806 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2847 proteins (977413 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski