Arthromitus sp. (strain SFB-mouse-Japan)
Average proteome isoelectric point is 6.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1487 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F9VJJ1|F9VJJ1_ARTSS Uncharacterized protein OS=Arthromitus sp. (strain SFB-mouse-Japan) OX=1029718 GN=SFBM_0521 PE=4 SV=1
MM1 pKa = 7.61 FDD3 pKa = 5.6 SIDD6 pKa = 3.83 DD7 pKa = 3.92 LLNYY11 pKa = 9.36 IRR13 pKa = 11.84 SNDD16 pKa = 3.18 TDD18 pKa = 4.22 LSVTYY23 pKa = 10.08 DD24 pKa = 3.16 ISSGNLLVMNNVTNQILFNQSLNYY48 pKa = 10.16 EE49 pKa = 3.71 IGEE52 pKa = 4.29 FKK54 pKa = 10.67 TSIEE58 pKa = 3.98 PLFTHH63 pKa = 6.75 LNCDD67 pKa = 3.02 VVYY70 pKa = 11.23 VKK72 pKa = 10.14 DD73 pKa = 3.56 TLTT76 pKa = 3.41
Molecular weight: 8.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.747
IPC2_protein 3.859
IPC_protein 3.77
Toseland 3.567
ProMoST 3.973
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.77
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.923
DTASelect 4.164
Thurlkill 3.656
EMBOSS 3.77
Sillero 3.897
Patrickios 0.693
IPC_peptide 3.757
IPC2_peptide 3.872
IPC2.peptide.svr19 3.805
Protein with the highest isoelectric point:
>tr|F9VM00|F9VM00_ARTSS Elongation factor G OS=Arthromitus sp. (strain SFB-mouse-Japan) OX=1029718 GN=fus PE=3 SV=1
MM1 pKa = 7.34 ARR3 pKa = 11.84 ISGVDD8 pKa = 3.07 IPKK11 pKa = 10.34 DD12 pKa = 3.27 KK13 pKa = 10.68 RR14 pKa = 11.84 VEE16 pKa = 3.89 VSLTYY21 pKa = 9.98 IYY23 pKa = 10.75 GIGLSTSKK31 pKa = 10.73 RR32 pKa = 11.84 ILKK35 pKa = 8.4 EE36 pKa = 3.45 TDD38 pKa = 2.67 INPNIRR44 pKa = 11.84 VKK46 pKa = 10.82 DD47 pKa = 3.61 LTEE50 pKa = 3.89 EE51 pKa = 3.99 QVNLIRR57 pKa = 11.84 DD58 pKa = 4.99 FINKK62 pKa = 8.46 NLRR65 pKa = 11.84 VEE67 pKa = 4.16 GDD69 pKa = 3.32 LRR71 pKa = 11.84 RR72 pKa = 11.84 DD73 pKa = 2.85 IVLNIKK79 pKa = 10.16 RR80 pKa = 11.84 LIEE83 pKa = 3.9 IGCYY87 pKa = 9.39 RR88 pKa = 11.84 GIRR91 pKa = 11.84 HH92 pKa = 6.76 RR93 pKa = 11.84 KK94 pKa = 8.24 RR95 pKa = 11.84 LPLRR99 pKa = 11.84 GQRR102 pKa = 11.84 TKK104 pKa = 10.95 TNARR108 pKa = 11.84 TIKK111 pKa = 10.5 GPRR114 pKa = 11.84 KK115 pKa = 8.34 TIANKK120 pKa = 10.18 KK121 pKa = 8.82 KK122 pKa = 10.46
Molecular weight: 14.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.31
IPC2_protein 9.823
IPC_protein 10.687
Toseland 11.067
ProMoST 10.891
Dawson 11.125
Bjellqvist 10.847
Wikipedia 11.359
Rodwell 11.374
Grimsley 11.155
Solomon 11.316
Lehninger 11.272
Nozaki 11.052
DTASelect 10.847
Thurlkill 11.052
EMBOSS 11.491
Sillero 11.067
Patrickios 11.096
IPC_peptide 11.33
IPC2_peptide 9.75
IPC2.peptide.svr19 8.663
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1487
0
1487
473593
36
2749
318.5
36.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.859 ± 0.059
1.221 ± 0.024
6.307 ± 0.053
6.653 ± 0.068
5.203 ± 0.056
5.747 ± 0.058
1.291 ± 0.023
11.112 ± 0.093
8.771 ± 0.068
9.097 ± 0.065
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.285 ± 0.026
7.715 ± 0.065
2.371 ± 0.037
2.099 ± 0.031
3.527 ± 0.038
7.287 ± 0.06
4.467 ± 0.068
6.006 ± 0.048
0.493 ± 0.017
4.489 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here