Winogradskyella sp. PG-2

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Winogradskyella; unclassified Winogradskyella

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3520 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A024FL28|A0A024FL28_9FLAO Uncharacterized protein OS=Winogradskyella sp. PG-2 OX=754409 GN=WPG_3344 PE=4 SV=1
MM1 pKa = 7.97RR2 pKa = 11.84KK3 pKa = 8.93ILLFNVLCFVINNVFGQISLDD24 pKa = 3.62DD25 pKa = 3.66RR26 pKa = 11.84VNIYY30 pKa = 10.94DD31 pKa = 3.98FDD33 pKa = 4.26SLSVNIIYY41 pKa = 10.42DD42 pKa = 3.75DD43 pKa = 3.89FDD45 pKa = 5.24NDD47 pKa = 3.42GDD49 pKa = 4.3LDD51 pKa = 3.61IMKK54 pKa = 10.48FSGTDD59 pKa = 3.05SFNVLLQKK67 pKa = 10.86NNNGDD72 pKa = 3.62FNLNQPILVSSGVRR86 pKa = 11.84PIVSLDD92 pKa = 3.31LDD94 pKa = 3.62NDD96 pKa = 3.85GFLDD100 pKa = 3.9LLTYY104 pKa = 10.75HH105 pKa = 7.0SFTTIGVLYY114 pKa = 9.53NQQDD118 pKa = 3.49NTFSEE123 pKa = 4.88EE124 pKa = 4.01VTVQTFNGSYY134 pKa = 9.94DD135 pKa = 3.28IHH137 pKa = 6.91PMKK140 pKa = 10.46FDD142 pKa = 3.84YY143 pKa = 11.5NNDD146 pKa = 2.78GFMDD150 pKa = 5.67LIAIDD155 pKa = 4.62DD156 pKa = 4.12SEE158 pKa = 4.29DD159 pKa = 3.75AYY161 pKa = 11.88VLINNQMGGLEE172 pKa = 4.17PPQFLVSVGTFDD184 pKa = 5.01FLYY187 pKa = 11.05DD188 pKa = 5.32LDD190 pKa = 5.96DD191 pKa = 4.69FDD193 pKa = 6.32NDD195 pKa = 3.63GDD197 pKa = 3.86FDD199 pKa = 5.09FYY201 pKa = 11.05IRR203 pKa = 11.84DD204 pKa = 3.6GDD206 pKa = 3.93KK207 pKa = 11.24LIININGSGVFDD219 pKa = 3.83QPGSQQTQSSLRR231 pKa = 11.84SFEE234 pKa = 4.15ILDD237 pKa = 3.52IDD239 pKa = 3.92GNGYY243 pKa = 10.48KK244 pKa = 10.34DD245 pKa = 3.35VLYY248 pKa = 10.08WKK250 pKa = 10.51NDD252 pKa = 4.3AIWAKK257 pKa = 10.52YY258 pKa = 9.27YY259 pKa = 10.78GYY261 pKa = 10.87NATTTRR267 pKa = 11.84FIVINDD273 pKa = 3.29VMVVNNIPKK282 pKa = 10.04FSNYY286 pKa = 8.08VNARR290 pKa = 11.84SIHH293 pKa = 7.59IEE295 pKa = 3.95DD296 pKa = 3.95QDD298 pKa = 3.89NGSHH302 pKa = 6.87DD303 pKa = 4.12VYY305 pKa = 10.69VTLATIEE312 pKa = 4.37NQLDD316 pKa = 3.37MHH318 pKa = 6.92KK319 pKa = 10.88FNIQNNVFSNAQIVLSDD336 pKa = 3.29FQVNVFGVDD345 pKa = 3.03EE346 pKa = 5.26FSFLDD351 pKa = 4.83LNNDD355 pKa = 3.63DD356 pKa = 5.23NLDD359 pKa = 3.65FSFVSNFNQNKK370 pKa = 10.02FIFINNNINDD380 pKa = 4.3TPDD383 pKa = 2.96KK384 pKa = 10.2TICIQQAVKK393 pKa = 10.58PNDD396 pKa = 3.81FYY398 pKa = 11.79VIDD401 pKa = 3.89MNGDD405 pKa = 3.61GEE407 pKa = 4.53NDD409 pKa = 3.12ICVGTQNGLGYY420 pKa = 10.07FEE422 pKa = 4.38KK423 pKa = 10.04TASNEE428 pKa = 3.79LSGLRR433 pKa = 11.84SLIGVSTNPNASAYY447 pKa = 6.41THH449 pKa = 7.04INITDD454 pKa = 3.51INNDD458 pKa = 3.16GLGDD462 pKa = 3.88VIDD465 pKa = 3.77YY466 pKa = 10.08TGFDD470 pKa = 3.49NEE472 pKa = 4.43AKK474 pKa = 10.19IFKK477 pKa = 10.5NLGDD481 pKa = 4.17DD482 pKa = 3.24NFEE485 pKa = 4.28FVQSVSMTGIFNGTHH500 pKa = 6.82LSFADD505 pKa = 3.64IDD507 pKa = 4.12GDD509 pKa = 3.98DD510 pKa = 4.37FKK512 pKa = 11.78DD513 pKa = 4.51LVIFDD518 pKa = 3.88QPNFYY523 pKa = 8.78WAKK526 pKa = 10.11NNNGLSFQSLQQLVVNNVDD545 pKa = 3.31NDD547 pKa = 3.98DD548 pKa = 4.65PLSIMYY554 pKa = 10.42EE555 pKa = 4.02DD556 pKa = 5.25FNDD559 pKa = 4.15DD560 pKa = 4.02DD561 pKa = 5.44EE562 pKa = 6.6IDD564 pKa = 3.8ILVLRR569 pKa = 11.84YY570 pKa = 9.25FYY572 pKa = 10.97EE573 pKa = 4.2SGQFHH578 pKa = 6.3TEE580 pKa = 3.77VNLLEE585 pKa = 4.35NDD587 pKa = 3.26NGEE590 pKa = 4.07FTGNIIADD598 pKa = 3.57FNGNYY603 pKa = 9.25GRR605 pKa = 11.84SHH607 pKa = 6.99LKK609 pKa = 9.91IDD611 pKa = 5.96DD612 pKa = 3.95FDD614 pKa = 3.91QDD616 pKa = 2.99NDD618 pKa = 3.35LDD620 pKa = 4.31FFVHH624 pKa = 5.76STRR627 pKa = 11.84LDD629 pKa = 3.42QPLLFFKK636 pKa = 11.01NDD638 pKa = 2.95GSNNFISTTIDD649 pKa = 3.42NIDD652 pKa = 3.33IEE654 pKa = 5.54DD655 pKa = 3.53IEE657 pKa = 5.58FYY659 pKa = 11.08DD660 pKa = 5.13GDD662 pKa = 3.74GDD664 pKa = 4.38GYY666 pKa = 11.45NEE668 pKa = 3.8IYY670 pKa = 10.02AWNYY674 pKa = 10.01EE675 pKa = 4.05SFANHH680 pKa = 5.94IFYY683 pKa = 10.85YY684 pKa = 10.0DD685 pKa = 3.26TTDD688 pKa = 3.51YY689 pKa = 11.54INFTKK694 pKa = 10.3IPIDD698 pKa = 3.88SYY700 pKa = 11.17SASYY704 pKa = 11.32DD705 pKa = 3.41NLDD708 pKa = 3.78DD709 pKa = 3.78YY710 pKa = 11.83TRR712 pKa = 11.84GDD714 pKa = 3.74LFMYY718 pKa = 10.18DD719 pKa = 3.82YY720 pKa = 11.76NNDD723 pKa = 3.36GKK725 pKa = 10.76DD726 pKa = 3.41DD727 pKa = 4.31LFINNYY733 pKa = 10.29SSFQALISVYY743 pKa = 10.31EE744 pKa = 4.09NTSEE748 pKa = 4.11SLGVEE753 pKa = 4.07EE754 pKa = 5.24IDD756 pKa = 4.46NNSLSQMRR764 pKa = 11.84LYY766 pKa = 10.33PNPFVNSVKK775 pKa = 8.1WTKK778 pKa = 10.43RR779 pKa = 11.84GNEE782 pKa = 4.14TYY784 pKa = 10.85SLQLYY789 pKa = 9.12SQDD792 pKa = 2.79GKK794 pKa = 11.48LIFEE798 pKa = 4.63KK799 pKa = 10.82VISEE803 pKa = 4.11NNMDD807 pKa = 4.12FSSYY811 pKa = 11.24DD812 pKa = 3.12SGVYY816 pKa = 9.58FLSISEE822 pKa = 4.39VISGNKK828 pKa = 7.89KK829 pKa = 6.1TFKK832 pKa = 10.28IIKK835 pKa = 9.12NN836 pKa = 3.59

Molecular weight: 95.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A024FKC4|A0A024FKC4_9FLAO Diacylglycerol kinase OS=Winogradskyella sp. PG-2 OX=754409 GN=WPG_2743 PE=3 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.06RR4 pKa = 11.84TFQPSKK10 pKa = 10.15RR11 pKa = 11.84KK12 pKa = 9.6RR13 pKa = 11.84KK14 pKa = 8.29NKK16 pKa = 9.34HH17 pKa = 4.03GFRR20 pKa = 11.84EE21 pKa = 4.05RR22 pKa = 11.84MASVNGRR29 pKa = 11.84KK30 pKa = 9.21VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.62LSVSSEE48 pKa = 4.04TRR50 pKa = 11.84HH51 pKa = 5.93KK52 pKa = 10.8RR53 pKa = 3.27

Molecular weight: 6.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3520

0

3520

1145739

37

3972

325.5

36.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.043 ± 0.037

0.738 ± 0.015

5.998 ± 0.048

6.407 ± 0.04

5.259 ± 0.037

6.286 ± 0.046

1.672 ± 0.021

8.29 ± 0.039

7.696 ± 0.081

9.201 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.094 ± 0.025

6.677 ± 0.046

3.189 ± 0.028

3.158 ± 0.022

3.308 ± 0.036

6.794 ± 0.038

5.913 ± 0.062

6.042 ± 0.032

1.042 ± 0.014

4.196 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski