Alkaliphilus peptidifermentans DSM 18978

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Alkaliphilus; Alkaliphilus peptidifermentans

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4235 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5FI90|A0A1G5FI90_9CLOT NAD(P)H-dependent flavin oxidoreductase YrpB nitropropane dioxygenase family OS=Alkaliphilus peptidifermentans DSM 18978 OX=1120976 GN=SAMN03080606_01429 PE=4 SV=1
MM1 pKa = 7.3KK2 pKa = 10.57GKK4 pKa = 9.7VKK6 pKa = 10.61KK7 pKa = 10.62SIVLIMVLALIFNGFAVTYY26 pKa = 10.22AGTVPSNLDD35 pKa = 3.38DD36 pKa = 6.3DD37 pKa = 4.3NEE39 pKa = 4.25EE40 pKa = 4.16WVDD43 pKa = 3.58TQTQDD48 pKa = 2.88EE49 pKa = 4.76FNSGEE54 pKa = 4.05EE55 pKa = 4.27DD56 pKa = 3.69NDD58 pKa = 5.01GEE60 pKa = 4.45DD61 pKa = 3.4TDD63 pKa = 4.68KK64 pKa = 11.68PEE66 pKa = 4.43DD67 pKa = 3.53LVGEE71 pKa = 4.44EE72 pKa = 4.85EE73 pKa = 4.2NSEE76 pKa = 4.63DD77 pKa = 3.68PTDD80 pKa = 3.62TDD82 pKa = 4.88GEE84 pKa = 4.54DD85 pKa = 3.8EE86 pKa = 4.23NSEE89 pKa = 4.53DD90 pKa = 4.41PADD93 pKa = 3.71TDD95 pKa = 4.81GEE97 pKa = 4.52DD98 pKa = 4.06EE99 pKa = 4.25NSEE102 pKa = 4.53DD103 pKa = 4.41PADD106 pKa = 3.71TDD108 pKa = 4.81GEE110 pKa = 4.52DD111 pKa = 4.06EE112 pKa = 4.25NSEE115 pKa = 4.53DD116 pKa = 4.41PADD119 pKa = 3.75TDD121 pKa = 3.97GEE123 pKa = 4.51EE124 pKa = 4.56EE125 pKa = 4.12NSEE128 pKa = 4.46DD129 pKa = 3.79PTDD132 pKa = 3.55TDD134 pKa = 3.43EE135 pKa = 4.64EE136 pKa = 4.57EE137 pKa = 5.04EE138 pKa = 4.15ISEE141 pKa = 4.76DD142 pKa = 3.58PTDD145 pKa = 3.86LEE147 pKa = 4.36EE148 pKa = 4.59VEE150 pKa = 4.93EE151 pKa = 4.09EE152 pKa = 4.39LGTGTGTGTGTGIVVPVPVYY172 pKa = 9.68EE173 pKa = 3.94RR174 pKa = 11.84PYY176 pKa = 9.16VINYY180 pKa = 9.08LVEE183 pKa = 4.19ATEE186 pKa = 4.44DD187 pKa = 3.69SVPGLEE193 pKa = 4.0SLYY196 pKa = 11.18GFGQVGDD203 pKa = 4.38TIEE206 pKa = 3.86VLYY209 pKa = 10.15PEE211 pKa = 4.17IEE213 pKa = 4.6GYY215 pKa = 10.02FVLEE219 pKa = 4.01NQPVSFVIVEE229 pKa = 4.02EE230 pKa = 4.39EE231 pKa = 3.92EE232 pKa = 3.99FNIINVYY239 pKa = 7.76YY240 pKa = 10.43GKK242 pKa = 10.9VIVIYY247 pKa = 9.66EE248 pKa = 4.16KK249 pKa = 11.0ASLTVNHH256 pKa = 6.68ILVSEE261 pKa = 4.28GGEE264 pKa = 3.99VLIDD268 pKa = 3.46TTLLEE273 pKa = 4.18GLEE276 pKa = 4.22IGAHH280 pKa = 5.25VNLLDD285 pKa = 4.23FAKK288 pKa = 10.63EE289 pKa = 3.86DD290 pKa = 3.64DD291 pKa = 4.11TMEE294 pKa = 4.5LLYY297 pKa = 11.2DD298 pKa = 3.61NLEE301 pKa = 4.39EE302 pKa = 4.26IFLTEE307 pKa = 4.18EE308 pKa = 3.86EE309 pKa = 4.36NLVNLYY315 pKa = 8.07YY316 pKa = 10.2QKK318 pKa = 11.1KK319 pKa = 8.61PGNEE323 pKa = 3.32QFYY326 pKa = 10.05VINYY330 pKa = 8.83LVEE333 pKa = 4.25STEE336 pKa = 4.2DD337 pKa = 3.51SVPGLDD343 pKa = 3.91SISGFGEE350 pKa = 3.86VGDD353 pKa = 4.54TIEE356 pKa = 3.62ILYY359 pKa = 9.4PEE361 pKa = 4.31RR362 pKa = 11.84EE363 pKa = 4.62GYY365 pKa = 9.75FVLEE369 pKa = 4.05NQPVSFVIVEE379 pKa = 4.02EE380 pKa = 4.39EE381 pKa = 3.92EE382 pKa = 3.99FNIINVYY389 pKa = 8.29YY390 pKa = 10.77GKK392 pKa = 8.94VTVIYY397 pKa = 9.38EE398 pKa = 4.22KK399 pKa = 11.09ASLTVNHH406 pKa = 6.68ILVSEE411 pKa = 4.28GGEE414 pKa = 3.99VLIDD418 pKa = 3.46TTLLEE423 pKa = 4.18GLEE426 pKa = 4.31IGAHH430 pKa = 4.53VNPLNFVKK438 pKa = 10.53EE439 pKa = 4.14DD440 pKa = 3.44DD441 pKa = 4.08TMEE444 pKa = 4.52LLYY447 pKa = 11.01DD448 pKa = 3.58SLEE451 pKa = 4.27EE452 pKa = 3.9IFLSEE457 pKa = 4.29EE458 pKa = 3.75EE459 pKa = 4.07NLINLYY465 pKa = 10.53YY466 pKa = 10.04YY467 pKa = 7.97EE468 pKa = 5.23KK469 pKa = 10.58PVAQDD474 pKa = 3.27FYY476 pKa = 10.44YY477 pKa = 10.38PIMPMMMRR485 pKa = 11.84FSTLSYY491 pKa = 8.59GTEE494 pKa = 3.86WPNPGAIKK502 pKa = 9.13LTKK505 pKa = 9.74EE506 pKa = 3.98AEE508 pKa = 4.08PVQDD512 pKa = 5.29SEE514 pKa = 4.54NSWEE518 pKa = 4.08VTLTIEE524 pKa = 4.62GKK526 pKa = 10.2NIQSSSKK533 pKa = 9.96VVLVIDD539 pKa = 4.52KK540 pKa = 9.98SGSMGPGYY548 pKa = 10.78GSGDD552 pKa = 2.85RR553 pKa = 11.84MVNTKK558 pKa = 10.0LAAKK562 pKa = 9.76EE563 pKa = 3.92FVDD566 pKa = 5.82RR567 pKa = 11.84LLLDD571 pKa = 4.54DD572 pKa = 4.93GATEE576 pKa = 4.0IAVVTFDD583 pKa = 4.38QNPQVVANFTSFEE596 pKa = 4.45DD597 pKa = 4.04KK598 pKa = 10.11DD599 pKa = 3.97TLKK602 pKa = 11.05AAIDD606 pKa = 5.06GIQADD611 pKa = 4.19GGTNIQAGIRR621 pKa = 11.84QAQILLDD628 pKa = 4.87AVDD631 pKa = 4.45ADD633 pKa = 3.86NKK635 pKa = 10.43VMVILGDD642 pKa = 3.82GEE644 pKa = 4.19PTYY647 pKa = 10.75SYY649 pKa = 10.94RR650 pKa = 11.84VTGVSGITLTCSFWSGHH667 pKa = 4.65TFNYY671 pKa = 10.44DD672 pKa = 3.01NAEE675 pKa = 3.89ITSFNYY681 pKa = 9.78NQIRR685 pKa = 11.84GNGRR689 pKa = 11.84DD690 pKa = 3.44YY691 pKa = 11.35SLPSNHH697 pKa = 6.95RR698 pKa = 11.84YY699 pKa = 9.68SVTIPCSTWGHH710 pKa = 6.16GSHH713 pKa = 5.87TTTYY717 pKa = 10.12PNDD720 pKa = 3.37NGIPTIYY727 pKa = 10.28EE728 pKa = 3.75AGLAKK733 pKa = 10.71DD734 pKa = 4.21KK735 pKa = 10.81GTDD738 pKa = 2.88IYY740 pKa = 11.23SIALEE745 pKa = 4.27ADD747 pKa = 3.23ANGQAVLNSIQNKK760 pKa = 9.8GYY762 pKa = 10.02YY763 pKa = 8.91QLNNSNLSGLEE774 pKa = 3.75AVFNEE779 pKa = 3.94IAGSITYY786 pKa = 9.45AASNGIVVDD795 pKa = 3.7PMGTMFDD802 pKa = 4.89LISSEE807 pKa = 3.96AEE809 pKa = 3.71IYY811 pKa = 10.44VSQGSVTINPANTVNWNVGNVAEE834 pKa = 4.54GTPAILTYY842 pKa = 10.3IVQIKK847 pKa = 10.28SDD849 pKa = 3.21ADD851 pKa = 3.71ANVMYY856 pKa = 7.41PTNDD860 pKa = 3.09TTTFTYY866 pKa = 10.71TDD868 pKa = 3.02VDD870 pKa = 3.94GNTVEE875 pKa = 5.55DD876 pKa = 4.11EE877 pKa = 4.05FDD879 pKa = 3.4IPEE882 pKa = 3.8VSIAGGRR889 pKa = 11.84ILLKK893 pKa = 10.69GYY895 pKa = 9.93LVNDD899 pKa = 3.53NGDD902 pKa = 4.43PINKK906 pKa = 9.63NGVVVDD912 pKa = 4.83RR913 pKa = 11.84PDD915 pKa = 3.16LAYY918 pKa = 10.02KK919 pKa = 10.69LFDD922 pKa = 3.5KK923 pKa = 10.18EE924 pKa = 4.56YY925 pKa = 9.07PGSPLGFNEE934 pKa = 4.85VYY936 pKa = 10.16TVTQPGAEE944 pKa = 4.48GYY946 pKa = 10.39HH947 pKa = 4.72YY948 pKa = 10.98LKK950 pKa = 10.92YY951 pKa = 10.6VWNNSTGSDD960 pKa = 3.4EE961 pKa = 4.47TVSVLLQASKK971 pKa = 9.02PTEE974 pKa = 4.06IVWFGYY980 pKa = 10.44RR981 pKa = 11.84EE982 pKa = 4.09VAEE985 pKa = 4.18LEE987 pKa = 4.43YY988 pKa = 10.6TVEE991 pKa = 4.22YY992 pKa = 10.11YY993 pKa = 10.21INGQGEE999 pKa = 4.42PFATIAGEE1007 pKa = 4.28VPVANPVVGSVSYY1020 pKa = 10.05EE1021 pKa = 3.97NKK1023 pKa = 8.61PAGYY1027 pKa = 9.69KK1028 pKa = 9.99

Molecular weight: 113.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5J060|A0A1G5J060_9CLOT Carboxypeptidase PM20D1 OS=Alkaliphilus peptidifermentans DSM 18978 OX=1120976 GN=SAMN03080606_02591 PE=4 SV=1
MM1 pKa = 7.23ANKK4 pKa = 9.42MKK6 pKa = 8.8THH8 pKa = 6.61KK9 pKa = 10.56GAAKK13 pKa = 10.1RR14 pKa = 11.84LKK16 pKa = 8.83KK17 pKa = 9.16TKK19 pKa = 9.89SGKK22 pKa = 9.72LKK24 pKa = 10.26RR25 pKa = 11.84FKK27 pKa = 11.01AFTSHH32 pKa = 7.4ILTKK36 pKa = 10.5KK37 pKa = 7.85SAKK40 pKa = 9.7RR41 pKa = 11.84KK42 pKa = 8.86RR43 pKa = 11.84NLRR46 pKa = 11.84KK47 pKa = 9.38AAIVSRR53 pKa = 11.84GDD55 pKa = 3.08QRR57 pKa = 11.84RR58 pKa = 11.84IQQLLPP64 pKa = 3.77

Molecular weight: 7.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4235

0

4235

1275674

39

2851

301.2

33.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.091 ± 0.036

0.958 ± 0.014

5.284 ± 0.027

7.498 ± 0.04

4.266 ± 0.031

6.77 ± 0.037

1.71 ± 0.016

9.946 ± 0.039

7.61 ± 0.037

9.42 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.016

5.468 ± 0.029

3.159 ± 0.022

2.904 ± 0.022

3.767 ± 0.023

5.973 ± 0.03

5.126 ± 0.031

6.542 ± 0.029

0.801 ± 0.012

3.935 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski