Paenibacillus piri
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6802 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V2ZTS5|A0A4V2ZTS5_9BACL Uncharacterized protein OS=Paenibacillus piri OX=2547395 GN=E1757_11950 PE=4 SV=1
MM1 pKa = 6.95 NAEE4 pKa = 4.48 AIFFEE9 pKa = 4.47 FDD11 pKa = 3.18 NEE13 pKa = 3.86 ASALLAQSTLEE24 pKa = 3.99 EE25 pKa = 4.1 LGYY28 pKa = 8.7 TAGLHH33 pKa = 6.56 SEE35 pKa = 4.43 FNHH38 pKa = 5.86 PTLHH42 pKa = 6.03 IQVEE46 pKa = 4.44 HH47 pKa = 6.34 SVLSSALEE55 pKa = 3.65 IAQAHH60 pKa = 6.68 GGRR63 pKa = 11.84 LVEE66 pKa = 4.3 HH67 pKa = 6.54 TSGLTEE73 pKa = 3.79 NDD75 pKa = 4.02 AYY77 pKa = 10.61 RR78 pKa = 11.84 QAYY81 pKa = 7.62 DD82 pKa = 3.58 TDD84 pKa = 4.05 DD85 pKa = 4.57 GYY87 pKa = 10.95 ISIPAHH93 pKa = 5.39 VVNEE97 pKa = 4.26 DD98 pKa = 2.75 WSEE101 pKa = 4.09 LYY103 pKa = 9.17 ATNAEE108 pKa = 4.44 NADD111 pKa = 3.63 SLYY114 pKa = 11.1 APDD117 pKa = 4.65 RR118 pKa = 11.84 GADD121 pKa = 3.36 GAAAGDD127 pKa = 4.13 SDD129 pKa = 5.94 DD130 pKa = 5.48 SGNSDD135 pKa = 3.6 NPVSGAAGEE144 pKa = 4.27 SDD146 pKa = 3.55 SGNGAKK152 pKa = 10.36 VFDD155 pKa = 5.17 PSDD158 pKa = 3.58 DD159 pKa = 4.4 DD160 pKa = 4.5 YY161 pKa = 12.08 SHH163 pKa = 7.34 FDD165 pKa = 2.94 AGVRR169 pKa = 11.84 LL170 pKa = 3.76
Molecular weight: 18.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.897
IPC_protein 3.897
Toseland 3.681
ProMoST 4.05
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.808
Rodwell 3.719
Grimsley 3.592
Solomon 3.872
Lehninger 3.821
Nozaki 3.986
DTASelect 4.215
Thurlkill 3.732
EMBOSS 3.821
Sillero 4.012
Patrickios 1.138
IPC_peptide 3.872
IPC2_peptide 3.999
IPC2.peptide.svr19 3.892
Protein with the highest isoelectric point:
>tr|A0A4R5KGH5|A0A4R5KGH5_9BACL Carbohydrate ABC transporter permease OS=Paenibacillus piri OX=2547395 GN=E1757_24110 PE=3 SV=1
MM1 pKa = 7.61 GPTFKK6 pKa = 10.92 PNVRR10 pKa = 11.84 KK11 pKa = 9.84 RR12 pKa = 11.84 KK13 pKa = 8.89 KK14 pKa = 8.64 VHH16 pKa = 5.57 GFRR19 pKa = 11.84 KK20 pKa = 9.99 RR21 pKa = 11.84 MSSKK25 pKa = 10.47 NGRR28 pKa = 11.84 KK29 pKa = 9.01 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 QKK37 pKa = 10.23 GRR39 pKa = 11.84 KK40 pKa = 8.56 VLSAA44 pKa = 4.05
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.705
Grimsley 12.91
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.427
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6802
0
6802
2221892
21
3639
326.7
36.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.534 ± 0.033
0.832 ± 0.009
5.08 ± 0.022
6.319 ± 0.033
4.213 ± 0.026
7.481 ± 0.03
2.14 ± 0.015
6.68 ± 0.029
5.394 ± 0.03
10.039 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.871 ± 0.014
3.784 ± 0.023
4.247 ± 0.019
3.981 ± 0.024
5.143 ± 0.03
6.208 ± 0.024
5.223 ± 0.027
7.008 ± 0.022
1.33 ± 0.013
3.492 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here