Paenarthrobacter aurescens (strain TC1)
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4565 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A1R6S7|A1R6S7_PAEAT Methyltransf_25 domain-containing protein OS=Paenarthrobacter aurescens (strain TC1) OX=290340 GN=AAur_2197 PE=4 SV=1
MM1 pKa = 8.26 SEE3 pKa = 3.96 EE4 pKa = 4.28 TKK6 pKa = 11.32 GEE8 pKa = 4.13 LVCNANDD15 pKa = 3.57 IQVKK19 pKa = 8.94 QALRR23 pKa = 11.84 VLIDD27 pKa = 4.6 DD28 pKa = 4.09 YY29 pKa = 11.51 PVAIVKK35 pKa = 10.6 DD36 pKa = 3.79 SMGDD40 pKa = 2.79 IHH42 pKa = 8.05 AIADD46 pKa = 4.08 TCSHH50 pKa = 6.72 ADD52 pKa = 3.01 ISLSEE57 pKa = 4.48 GEE59 pKa = 4.5 VEE61 pKa = 4.72 GCAIEE66 pKa = 4.32 CWGHH70 pKa = 6.51 GSQFDD75 pKa = 3.98 LRR77 pKa = 11.84 SGQPLQLPAYY87 pKa = 9.85 DD88 pKa = 4.19 PVPVFAVTIDD98 pKa = 3.64 GDD100 pKa = 3.97 DD101 pKa = 3.83 VYY103 pKa = 11.98 VDD105 pKa = 3.17 VTNVVNGASVDD116 pKa = 3.6 NYY118 pKa = 11.54
Molecular weight: 12.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.846
IPC_protein 3.821
Toseland 3.605
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.986
Wikipedia 3.783
Rodwell 3.656
Grimsley 3.516
Solomon 3.808
Lehninger 3.77
Nozaki 3.948
DTASelect 4.19
Thurlkill 3.668
EMBOSS 3.783
Sillero 3.948
Patrickios 0.655
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.852
Protein with the highest isoelectric point:
>sp|A1R8P8|COAA_PAEAT Pantothenate kinase OS=Paenarthrobacter aurescens (strain TC1) OX=290340 GN=coaA PE=3 SV=1
MM1 pKa = 7.61 KK2 pKa = 10.31 VRR4 pKa = 11.84 NSLRR8 pKa = 11.84 ALKK11 pKa = 9.65 KK12 pKa = 10.17 IPGAQIVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GRR24 pKa = 11.84 TFVINKK30 pKa = 8.58 NNPRR34 pKa = 11.84 MKK36 pKa = 10.38 ARR38 pKa = 11.84 QGG40 pKa = 3.24
Molecular weight: 4.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.096
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.574
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.31
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4565
0
4565
1514983
30
2109
331.9
35.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.477 ± 0.048
0.658 ± 0.009
5.554 ± 0.027
5.63 ± 0.037
3.286 ± 0.022
8.92 ± 0.032
2.133 ± 0.018
4.527 ± 0.028
2.976 ± 0.023
10.092 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.075 ± 0.015
2.631 ± 0.021
5.442 ± 0.028
3.187 ± 0.018
6.25 ± 0.034
6.214 ± 0.026
6.037 ± 0.028
8.336 ± 0.028
1.486 ± 0.014
2.089 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here