Sulfuritalea hydrogenivorans sk43H
Average proteome isoelectric point is 6.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3578 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W0SGV9|W0SGV9_9PROT 3-oxoacyl-[acyl-carrier-protein] reductase OS=Sulfuritalea hydrogenivorans sk43H OX=1223802 GN=fabG PE=3 SV=1
MM1 pKa = 7.7 GNFDD5 pKa = 3.99 LHH7 pKa = 7.65 GLINAIANNHH17 pKa = 5.08 GVASPVKK24 pKa = 8.74 SAKK27 pKa = 10.04 RR28 pKa = 11.84 KK29 pKa = 8.43 MRR31 pKa = 11.84 DD32 pKa = 3.02 VSEE35 pKa = 4.82 GDD37 pKa = 3.59 GQATAQAEE45 pKa = 4.23 GDD47 pKa = 3.79 ATSATSDD54 pKa = 3.18 ASMQLAQADD63 pKa = 3.76 TGVATEE69 pKa = 4.24 AAAGEE74 pKa = 4.54 TTGGASGAPAAGEE87 pKa = 3.84 AAAGSGAAAGGLGGLGLGGMALGGAALAGGGGGGGSAVAAAVNNIITGTVVAGPVLAGSGLKK149 pKa = 10.26 VEE151 pKa = 5.26 IYY153 pKa = 10.3 AANGTTKK160 pKa = 10.58 LGEE163 pKa = 4.29 STLDD167 pKa = 3.2 ASGKK171 pKa = 8.51 FTVDD175 pKa = 2.53 VGAYY179 pKa = 7.71 TGIVIARR186 pKa = 11.84 LVDD189 pKa = 3.69 ANGGDD194 pKa = 4.09 DD195 pKa = 4.33 YY196 pKa = 11.62 LDD198 pKa = 3.91 EE199 pKa = 4.51 ATGVPKK205 pKa = 10.61 DD206 pKa = 3.7 LNANLMATSVVTGGTFTLNINPLTTIAAQKK236 pKa = 10.66 AGLAADD242 pKa = 4.57 GSGGLADD249 pKa = 4.07 AAAVANANAAVAAAFGLTDD268 pKa = 3.61 LTGTSVVATNGGSYY282 pKa = 10.69 DD283 pKa = 3.6 AADD286 pKa = 3.51 GMSAGEE292 pKa = 4.2 KK293 pKa = 10.26 YY294 pKa = 10.62 GAILAALSGADD305 pKa = 3.73 FASSGDD311 pKa = 3.62 SQAIIDD317 pKa = 4.25 NLVAAIDD324 pKa = 3.52 ITGASGTLSAAGQSAVIAGAAVVDD348 pKa = 4.01 SDD350 pKa = 5.87 FIDD353 pKa = 4.71 DD354 pKa = 3.68 VLNTVRR360 pKa = 11.84 AGATAPPAPTINAVAADD377 pKa = 4.13 NIINADD383 pKa = 3.56 EE384 pKa = 4.31 VEE386 pKa = 4.38 SVITGTNEE394 pKa = 3.24 TGATVDD400 pKa = 4.51 LGIGGNTRR408 pKa = 11.84 AATVSGTTWSYY419 pKa = 10.08 TLTDD423 pKa = 3.69 ADD425 pKa = 4.43 LTAMGQGAEE434 pKa = 4.38 TLSATQTDD442 pKa = 3.96 VLGNTGSAGTRR453 pKa = 11.84 AISVDD458 pKa = 3.04 TVAPVFSSATVSGRR472 pKa = 11.84 TLVLTYY478 pKa = 11.04 NEE480 pKa = 4.53 ALDD483 pKa = 4.14 ATSMPLPSAFRR494 pKa = 11.84 VGGIAQGAPVAGVVDD509 pKa = 4.64 SAAKK513 pKa = 9.58 TLTLTLASPVTYY525 pKa = 9.23 GQTGITVSYY534 pKa = 10.26 TDD536 pKa = 3.51 PTEE539 pKa = 5.28 DD540 pKa = 3.55 NDD542 pKa = 4.1 LAAIQDD548 pKa = 4.04 LAGNDD553 pKa = 3.44 ASTLTNLAVTNNTPDD568 pKa = 3.2 APLYY572 pKa = 10.51 QSATVTGSTLVMTYY586 pKa = 10.81 AGDD589 pKa = 3.78 LDD591 pKa = 4.6 EE592 pKa = 6.14 NNLPPLDD599 pKa = 3.9 AFTVKK604 pKa = 10.4 VNNTAQAAPTGVVVDD619 pKa = 4.68 ADD621 pKa = 3.82 AGTVTLTLAAPVIHH635 pKa = 6.43 GQTVTVGYY643 pKa = 8.41 TDD645 pKa = 4.36 PSAANDD651 pKa = 3.53 LNAIQDD657 pKa = 3.62 SGLNDD662 pKa = 3.33 AVSIGNRR669 pKa = 11.84 VVANQTPDD677 pKa = 2.75 AAAPVFANAVVTEE690 pKa = 3.91 NGKK693 pKa = 9.65 YY694 pKa = 10.08 LVLNCDD700 pKa = 3.33 EE701 pKa = 5.13 ALDD704 pKa = 4.29 AAHH707 pKa = 6.49 VPPAGAFIVKK717 pKa = 10.12 VGGVAQATPTGLTVNASAKK736 pKa = 9.72 TVMLTLATPITFGQTGITVSYY757 pKa = 9.28 TDD759 pKa = 4.08 PSAANDD765 pKa = 3.09 INAIQDD771 pKa = 3.54 AVGNDD776 pKa = 3.44 AASVTDD782 pKa = 5.25 LPVTNNAADD791 pKa = 3.96 TPILEE796 pKa = 4.51 SATVHH801 pKa = 6.84 GDD803 pKa = 3.68 TLVLTYY809 pKa = 11.1 NDD811 pKa = 4.46 ALDD814 pKa = 4.3 AGNVPLPEE822 pKa = 4.26 AFAVKK827 pKa = 10.4 VGGADD832 pKa = 3.2 PVAPTNVAVDD842 pKa = 3.74 SAARR846 pKa = 11.84 TVTLTLADD854 pKa = 4.03 AVTSGQTVKK863 pKa = 10.73 LSYY866 pKa = 10.52 TDD868 pKa = 3.5 PTTDD872 pKa = 3.16 NDD874 pKa = 3.24 ADD876 pKa = 4.7 AIQDD880 pKa = 3.63 VAGNDD885 pKa = 3.2 AFTVEE890 pKa = 4.36 NQSVTNEE897 pKa = 4.06 TPPVFSAATVTGSTLVMNYY916 pKa = 10.13 SDD918 pKa = 5.06 ALDD921 pKa = 4.14 AAHH924 pKa = 6.62 VPPANAFTIEE934 pKa = 4.39 VNGTPQAAPSAVAVDD949 pKa = 3.74 AAARR953 pKa = 11.84 TVTLTLAAAVTNGQAVTVSYY973 pKa = 11.44 ADD975 pKa = 3.45 PTAGNDD981 pKa = 3.01 AAAIQNAIGSDD992 pKa = 3.47 AVSLSNQPVSNHH1004 pKa = 5.48 TPPLLSGATVSGDD1017 pKa = 3.65 TLVLTYY1023 pKa = 10.97 SNEE1026 pKa = 3.82 LDD1028 pKa = 3.55 EE1029 pKa = 6.02 SNIPLVNAFTVKK1041 pKa = 10.55 VNGVAQAAPTDD1052 pKa = 4.02 VVVDD1056 pKa = 4.13 AAARR1060 pKa = 11.84 TVTLTLATAVTDD1072 pKa = 4.34 GQAVTVSYY1080 pKa = 11.1 VDD1082 pKa = 4.05 PSTGDD1087 pKa = 3.31 DD1088 pKa = 3.47 TEE1090 pKa = 5.63 AIQDD1094 pKa = 3.91 ALGNDD1099 pKa = 4.55 AISVANRR1106 pKa = 11.84 PVTNTTADD1114 pKa = 3.0 VAAPVFSGATVSGDD1128 pKa = 3.6 TLVLSYY1134 pKa = 11.24 SEE1136 pKa = 5.19 ALDD1139 pKa = 3.79 AANAPVAGDD1148 pKa = 3.81 FIVNVDD1154 pKa = 3.48 GTAQADD1160 pKa = 3.68 PTAVVVDD1167 pKa = 4.05 ATARR1171 pKa = 11.84 TVTLTLAAAVAYY1183 pKa = 9.94 GEE1185 pKa = 4.55 AVTVTYY1191 pKa = 10.24 TDD1193 pKa = 3.51 PTVGDD1198 pKa = 4.36 DD1199 pKa = 3.29 ATAIQDD1205 pKa = 3.7 TAGNDD1210 pKa = 3.25 AVTLTEE1216 pKa = 3.78 QSVTNTSVNALAPTLSVVVSAGADD1240 pKa = 3.2 VNAIVLTVTTGLNGAASADD1259 pKa = 4.3 LIFDD1263 pKa = 4.56 GLPGGAVVRR1272 pKa = 11.84 NSAAVDD1278 pKa = 3.47 VTAGVVNFDD1287 pKa = 3.59 GSDD1290 pKa = 3.11 TFTVEE1295 pKa = 4.26 FPEE1298 pKa = 6.34 DD1299 pKa = 3.62 SDD1301 pKa = 3.27 IHH1303 pKa = 6.72 QNLLVTVTGKK1313 pKa = 10.32 DD1314 pKa = 3.11 AGGDD1318 pKa = 3.58 PLGDD1322 pKa = 3.43 NVHH1325 pKa = 6.68 TIDD1328 pKa = 4.84 LVYY1331 pKa = 10.83 DD1332 pKa = 3.88 VASSTEE1338 pKa = 3.87 DD1339 pKa = 3.68 LNFASANQNMWGNFNGYY1356 pKa = 9.3 IGWHH1360 pKa = 6.07 EE1361 pKa = 4.57 YY1362 pKa = 10.37 IPLLGDD1368 pKa = 4.76 APIVWNEE1375 pKa = 3.85 ATGEE1379 pKa = 3.92 WDD1381 pKa = 3.59 DD1382 pKa = 5.31 AANPDD1387 pKa = 3.61 YY1388 pKa = 10.25 WRR1390 pKa = 11.84 SGEE1393 pKa = 3.94 FSVIDD1398 pKa = 3.5 VDD1400 pKa = 4.82 LDD1402 pKa = 3.59 SSQVIEE1408 pKa = 4.32 AAAIAASSVLDD1419 pKa = 3.47 TAKK1422 pKa = 10.92 AVFDD1426 pKa = 3.94 VAAVAVDD1433 pKa = 4.24 TVAKK1437 pKa = 10.65 GIFDD1441 pKa = 3.54 TAKK1444 pKa = 10.77 AVFQTAEE1451 pKa = 3.51 NLYY1454 pKa = 9.26 YY1455 pKa = 10.69 FGARR1459 pKa = 11.84 TVDD1462 pKa = 3.27 AAVQATFQAAKK1473 pKa = 9.76 GAYY1476 pKa = 8.98 DD1477 pKa = 3.75 VAHH1480 pKa = 6.89 GLYY1483 pKa = 10.62 DD1484 pKa = 3.41 SASYY1488 pKa = 10.79 LFHH1491 pKa = 6.36 TVATGLHH1498 pKa = 5.76 DD1499 pKa = 4.56 AAWNAYY1505 pKa = 9.52 ASYY1508 pKa = 10.81 DD1509 pKa = 3.41 NWYY1512 pKa = 10.26 HH1513 pKa = 7.15 SLDD1516 pKa = 3.07 GWGQFWNAAGHH1527 pKa = 5.11 VANDD1531 pKa = 3.86 IAWGIADD1538 pKa = 3.35 VAYY1541 pKa = 10.81 NVAVPIWDD1549 pKa = 3.45 AAKK1552 pKa = 10.58 YY1553 pKa = 10.39 AFEE1556 pKa = 4.2 TTATGIYY1563 pKa = 7.95 NTALAVYY1570 pKa = 9.1 NAAAGVANSAAQVVFDD1586 pKa = 3.63 GAKK1589 pKa = 10.25 AVFATAEE1596 pKa = 3.96 HH1597 pKa = 6.71 VYY1599 pKa = 11.15 NEE1601 pKa = 4.33 VKK1603 pKa = 10.41 QDD1605 pKa = 3.27 IYY1607 pKa = 11.73 DD1608 pKa = 3.81 VAQGVYY1614 pKa = 10.49 DD1615 pKa = 4.18 LARR1618 pKa = 11.84 DD1619 pKa = 3.41 GVMAVLATINDD1630 pKa = 3.52 KK1631 pKa = 11.51 VEE1633 pKa = 3.81 FDD1635 pKa = 3.44 TALKK1639 pKa = 10.41 VDD1641 pKa = 4.17 SEE1643 pKa = 4.65 VFAQVGLQVDD1653 pKa = 4.94 FEE1655 pKa = 5.91 LDD1657 pKa = 3.42 MGSVDD1662 pKa = 5.51 ADD1664 pKa = 2.79 IDD1666 pKa = 4.07 YY1667 pKa = 11.14 QLTSATQYY1675 pKa = 11.32 NKK1677 pKa = 10.43 AADD1680 pKa = 4.07 MLTITPTMTNMTTGDD1695 pKa = 3.47 AVAFDD1700 pKa = 4.81 TISPNAKK1707 pKa = 9.63 FYY1709 pKa = 11.11 AALLYY1714 pKa = 10.72 DD1715 pKa = 3.75 VGADD1719 pKa = 3.19 FDD1721 pKa = 4.22 VFIDD1725 pKa = 4.02 GKK1727 pKa = 10.9 LVVDD1731 pKa = 3.59 NTTVYY1736 pKa = 10.82 DD1737 pKa = 4.62 LSPTSDD1743 pKa = 2.99 GLAIQFPISTEE1754 pKa = 3.9 SFADD1758 pKa = 3.57 SLSEE1762 pKa = 3.84 MTGGDD1767 pKa = 3.18 IEE1769 pKa = 4.36 VGKK1772 pKa = 9.5 MVLIDD1777 pKa = 4.84 LDD1779 pKa = 4.16 TTQLEE1784 pKa = 4.39 PFEE1787 pKa = 4.59 VPFVEE1792 pKa = 6.34 ALTQDD1797 pKa = 3.12 ILSIEE1802 pKa = 4.06 IAIPTLEE1809 pKa = 4.36 TEE1811 pKa = 4.88 GKK1813 pKa = 9.87 AATYY1817 pKa = 10.67 VPATADD1823 pKa = 2.51 IWTTGTFEE1831 pKa = 4.07 EE1832 pKa = 4.82 QIGPFPYY1839 pKa = 10.41 ASVDD1843 pKa = 3.84 FSEE1846 pKa = 4.57 ISSAFFNIINAKK1858 pKa = 9.91 FDD1860 pKa = 3.85 FSEE1863 pKa = 4.47 EE1864 pKa = 3.98 FMDD1867 pKa = 5.27 QYY1869 pKa = 11.94 GLDD1872 pKa = 4.0 SLGDD1876 pKa = 3.48 AATLAEE1882 pKa = 4.49 TVQDD1886 pKa = 3.27 IATGLMANIWDD1897 pKa = 4.22 TLDD1900 pKa = 3.87 GEE1902 pKa = 4.67 AEE1904 pKa = 4.14 GVPIFVLDD1912 pKa = 3.8 MTDD1915 pKa = 3.21 EE1916 pKa = 4.37 TASSLLHH1923 pKa = 6.63 LNLFTDD1929 pKa = 4.11 DD1930 pKa = 3.69 VMGNTTSANTGSLGFYY1946 pKa = 9.86 AAYY1949 pKa = 10.25 GEE1951 pKa = 4.43 SDD1953 pKa = 3.66 PVVAVTVDD1961 pKa = 3.7 LDD1963 pKa = 3.48 AAYY1966 pKa = 10.53 VAIVKK1971 pKa = 10.4 AIAKK1975 pKa = 9.07 AAASAVTAGAATTIHH1990 pKa = 6.38 PVIDD1994 pKa = 5.9 AIPSPYY2000 pKa = 10.04 NLEE2003 pKa = 4.19 FGVEE2007 pKa = 4.05 QVLEE2011 pKa = 4.19 IAAVPQATIDD2021 pKa = 5.64 QITQWLNLGFTFEE2034 pKa = 4.71 AADD2037 pKa = 4.08 LDD2039 pKa = 3.95 VTQAVNFSQDD2049 pKa = 3.05 FTLSIDD2055 pKa = 3.56 DD2056 pKa = 3.57 MSYY2059 pKa = 11.29 LVTLEE2064 pKa = 5.09 DD2065 pKa = 3.7 NTTHH2069 pKa = 7.44 AFTANGTGALQISNAGSHH2087 pKa = 6.8 DD2088 pKa = 4.03 ANHH2091 pKa = 7.35 DD2092 pKa = 3.58 GLITYY2097 pKa = 7.97 TLDD2100 pKa = 3.13 IVPTAMFSNDD2110 pKa = 3.37 TEE2112 pKa = 4.47 VGLSLGYY2119 pKa = 10.4 QLDD2122 pKa = 3.86 FLKK2125 pKa = 11.17 GGFSAGAQLPLGEE2138 pKa = 4.92 LLGLTDD2144 pKa = 5.76 ADD2146 pKa = 3.71 WLNIDD2151 pKa = 4.79 FSAIDD2156 pKa = 3.41 ISMGPLLRR2164 pKa = 11.84 VQGEE2168 pKa = 4.79 LDD2170 pKa = 3.55 TLDD2173 pKa = 3.29 VDD2175 pKa = 4.07 VFEE2178 pKa = 5.73 SRR2180 pKa = 11.84 FALDD2184 pKa = 3.31 VGSASYY2190 pKa = 10.91 AGAIDD2195 pKa = 3.45 IGLVNHH2201 pKa = 6.19 SVMVV2205 pKa = 3.78
Molecular weight: 223.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.516
IPC_protein 3.592
Toseland 3.35
ProMoST 3.77
Dawson 3.592
Bjellqvist 3.745
Wikipedia 3.554
Rodwell 3.401
Grimsley 3.249
Solomon 3.592
Lehninger 3.554
Nozaki 3.706
DTASelect 4.012
Thurlkill 3.401
EMBOSS 3.554
Sillero 3.719
Patrickios 1.494
IPC_peptide 3.592
IPC2_peptide 3.694
IPC2.peptide.svr19 3.685
Protein with the highest isoelectric point:
>tr|W0SL05|W0SL05_9PROT PAS/PAC sensor-containing diguanylate cyclase/phosphodiesterase OS=Sulfuritalea hydrogenivorans sk43H OX=1223802 GN=SUTH_02684 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.36 RR3 pKa = 11.84 TYY5 pKa = 9.97 QPSVVRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.6 RR14 pKa = 11.84 THH16 pKa = 5.72 GFLVRR21 pKa = 11.84 MRR23 pKa = 11.84 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVIRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.91 GRR39 pKa = 11.84 HH40 pKa = 4.92 RR41 pKa = 11.84 LAVV44 pKa = 3.37
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.461
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.34
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.076
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.116
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3578
0
3578
1166199
39
4918
325.9
35.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.399 ± 0.067
1.01 ± 0.017
5.375 ± 0.034
5.82 ± 0.041
3.668 ± 0.031
8.262 ± 0.054
2.227 ± 0.021
5.03 ± 0.031
3.917 ± 0.042
10.654 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.544 ± 0.02
2.785 ± 0.03
4.888 ± 0.033
3.469 ± 0.04
6.866 ± 0.055
5.192 ± 0.037
5.029 ± 0.072
7.242 ± 0.036
1.36 ± 0.018
2.262 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here