Tomato leaf curl Palampur virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3CJR4|B3CJR4_9GEMI AC4 OS=Tomato leaf curl Palampur virus OX=526476 GN=AC4 PE=3 SV=1
MM1 pKa = 7.69APPTRR6 pKa = 11.84FRR8 pKa = 11.84INAKK12 pKa = 10.24NYY14 pKa = 8.19FLTYY18 pKa = 9.06PKK20 pKa = 10.53CSLTKK25 pKa = 10.69EE26 pKa = 4.09EE27 pKa = 5.55ALSQLQNLEE36 pKa = 4.19TPTSKK41 pKa = 10.74KK42 pKa = 9.91FIKK45 pKa = 9.76ICRR48 pKa = 11.84EE49 pKa = 3.71LHH51 pKa = 6.27EE52 pKa = 5.93DD53 pKa = 4.19GSPHH57 pKa = 5.9IHH59 pKa = 6.36VLIQFEE65 pKa = 5.43GKK67 pKa = 9.21FQCKK71 pKa = 8.51NNRR74 pKa = 11.84FFDD77 pKa = 4.03LVAPSRR83 pKa = 11.84SAHH86 pKa = 5.25FHH88 pKa = 6.45PNIQGAKK95 pKa = 8.91SASDD99 pKa = 3.09VKK101 pKa = 10.46TYY103 pKa = 10.35IDD105 pKa = 3.55KK106 pKa = 11.35DD107 pKa = 3.67GDD109 pKa = 3.73VLEE112 pKa = 4.69WGVFQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 4.42AYY134 pKa = 10.55AQAINTGNKK143 pKa = 9.01EE144 pKa = 3.96DD145 pKa = 3.66ALKK148 pKa = 10.71VLKK151 pKa = 10.33EE152 pKa = 4.25LAPKK156 pKa = 10.39DD157 pKa = 3.72YY158 pKa = 11.2VLQFHH163 pKa = 6.89NLMTNLDD170 pKa = 4.22RR171 pKa = 11.84IFPSRR176 pKa = 11.84IEE178 pKa = 4.15VYY180 pKa = 10.27RR181 pKa = 11.84SPFTVSSFDD190 pKa = 3.31RR191 pKa = 11.84VPPEE195 pKa = 3.62LVDD198 pKa = 3.43WVSSNLRR205 pKa = 11.84CSAARR210 pKa = 11.84PFSAALPIRR219 pKa = 11.84PIGLVLEE226 pKa = 4.72GDD228 pKa = 3.67SRR230 pKa = 11.84TGKK233 pKa = 8.52TMWARR238 pKa = 11.84SLGPHH243 pKa = 6.71NYY245 pKa = 10.18LCGHH249 pKa = 7.35LDD251 pKa = 3.66LNPRR255 pKa = 11.84VYY257 pKa = 11.26SNDD260 pKa = 2.17AWYY263 pKa = 10.92NVIDD267 pKa = 5.2DD268 pKa = 4.41VDD270 pKa = 3.61PHH272 pKa = 5.85YY273 pKa = 11.0LKK275 pKa = 10.7HH276 pKa = 6.2FKK278 pKa = 10.7EE279 pKa = 4.46FMGAQRR285 pKa = 11.84DD286 pKa = 3.81WQSNTKK292 pKa = 9.53YY293 pKa = 9.97GKK295 pKa = 9.84PMMIKK300 pKa = 10.43GGIPTIFLCNKK311 pKa = 9.26GPQSSYY317 pKa = 11.54KK318 pKa = 10.5EE319 pKa = 3.91FLDD322 pKa = 3.79EE323 pKa = 4.63EE324 pKa = 4.37KK325 pKa = 11.01NAALKK330 pKa = 10.02QWALKK335 pKa = 9.87NAVFITLEE343 pKa = 4.21GPLYY347 pKa = 10.54SGRR350 pKa = 11.84EE351 pKa = 3.7NVAPQEE357 pKa = 4.23EE358 pKa = 4.97EE359 pKa = 4.04EE360 pKa = 4.23EE361 pKa = 4.57HH362 pKa = 5.95PQEE365 pKa = 4.27TSS367 pKa = 2.61

Molecular weight: 41.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3CL31|B3CL31_9GEMI Nuclear shuttle protein OS=Tomato leaf curl Palampur virus OX=526476 GN=BV1 PE=3 SV=1
MM1 pKa = 7.72GLRR4 pKa = 11.84ISMFSSNLKK13 pKa = 9.61EE14 pKa = 3.93NSSAKK19 pKa = 9.08ITDD22 pKa = 3.23SSTWLPQVGQHH33 pKa = 5.2ISIRR37 pKa = 11.84TFRR40 pKa = 11.84EE41 pKa = 3.51LNQRR45 pKa = 11.84QMSRR49 pKa = 11.84HH50 pKa = 5.35TSTKK54 pKa = 10.06TEE56 pKa = 4.11TFF58 pKa = 3.37

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1787

58

367

198.6

22.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.924 ± 0.643

2.182 ± 0.264

4.757 ± 0.379

5.148 ± 0.545

4.085 ± 0.3

5.484 ± 0.22

3.749 ± 0.714

5.54 ± 0.519

5.708 ± 0.421

7.219 ± 0.7

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.126 ± 0.371

5.26 ± 0.316

5.484 ± 0.477

3.47 ± 0.361

7.443 ± 0.643

8.618 ± 1.08

6.379 ± 0.718

7.107 ± 0.756

1.455 ± 0.109

3.861 ± 0.594
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski