Mariprofundus ferrinatatus
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2236 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2K8L3L8|A0A2K8L3L8_9PROT Uncharacterized protein OS=Mariprofundus ferrinatatus OX=1921087 GN=Ga0123462_1058 PE=4 SV=1
MM1 pKa = 7.51 QITDD5 pKa = 3.37 NKK7 pKa = 9.78 VVSFHH12 pKa = 5.84 YY13 pKa = 9.93 TLTNDD18 pKa = 3.0 AGEE21 pKa = 4.43 IVDD24 pKa = 4.07 TSAGGEE30 pKa = 4.02 PMPYY34 pKa = 10.34 LHH36 pKa = 6.95 GAANIVPGLEE46 pKa = 3.77 LALEE50 pKa = 4.68 GKK52 pKa = 10.25 RR53 pKa = 11.84 VGDD56 pKa = 3.68 KK57 pKa = 11.44 LNVTLEE63 pKa = 3.93 AADD66 pKa = 3.98 AYY68 pKa = 11.03 GEE70 pKa = 4.02 FDD72 pKa = 3.57 PALVEE77 pKa = 4.35 VVSAEE82 pKa = 4.06 LFDD85 pKa = 5.76 GIDD88 pKa = 3.63 NIEE91 pKa = 4.43 ADD93 pKa = 3.82 MEE95 pKa = 4.59 FEE97 pKa = 4.49 TEE99 pKa = 4.23 TEE101 pKa = 3.93 SGDD104 pKa = 3.4 IEE106 pKa = 4.48 FVRR109 pKa = 11.84 VTNVDD114 pKa = 3.18 GDD116 pKa = 4.23 NITIDD121 pKa = 3.93 GNHH124 pKa = 7.08 PLAGQNLTFAVEE136 pKa = 3.79 ITDD139 pKa = 3.62 IRR141 pKa = 11.84 DD142 pKa = 3.24 ATAEE146 pKa = 4.07 EE147 pKa = 4.88 LEE149 pKa = 4.59 HH150 pKa = 6.77 GHH152 pKa = 5.77 VHH154 pKa = 7.36 GDD156 pKa = 3.76 DD157 pKa = 6.07 DD158 pKa = 6.4 DD159 pKa = 5.86 DD160 pKa = 4.14 EE161 pKa = 5.69 CCGEE165 pKa = 4.15 GCGCHH170 pKa = 6.24
Molecular weight: 18.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.743
IPC2_protein 3.808
IPC_protein 3.795
Toseland 3.592
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.681
Rodwell 3.63
Grimsley 3.503
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.088
Thurlkill 3.63
EMBOSS 3.694
Sillero 3.91
Patrickios 1.863
IPC_peptide 3.77
IPC2_peptide 3.897
IPC2.peptide.svr19 3.829
Protein with the highest isoelectric point:
>tr|A0A2K8L7D7|A0A2K8L7D7_9PROT Uncharacterized protein OS=Mariprofundus ferrinatatus OX=1921087 GN=Ga0123462_0912 PE=4 SV=1
MM1 pKa = 7.5 SFTYY5 pKa = 9.9 KK6 pKa = 10.15 PSRR9 pKa = 11.84 IRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.68 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATRR25 pKa = 11.84 AGRR28 pKa = 11.84 AIINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.56 SLSVV44 pKa = 3.11
Molecular weight: 5.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.461
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.34
Grimsley 12.778
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.076
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.116
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2236
0
2236
707698
29
1550
316.5
34.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.947 ± 0.06
1.087 ± 0.021
5.597 ± 0.039
6.804 ± 0.045
3.723 ± 0.033
7.681 ± 0.044
2.508 ± 0.024
6.192 ± 0.04
4.516 ± 0.045
10.373 ± 0.072
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.978 ± 0.025
3.268 ± 0.031
4.176 ± 0.031
3.525 ± 0.032
5.958 ± 0.041
6.268 ± 0.036
4.62 ± 0.033
7.022 ± 0.045
1.248 ± 0.02
2.509 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here