Dermatophilus congolensis
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2311 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A239VKI9|A0A239VKI9_9MICO Ribosomal protein S12 methylthiotransferase RimO OS=Dermatophilus congolensis OX=1863 GN=rimO PE=3 SV=1
MM1 pKa = 7.73 RR2 pKa = 11.84 EE3 pKa = 3.74 WKK5 pKa = 10.34 QILGALWRR13 pKa = 11.84 VYY15 pKa = 9.04 WGCCGDD21 pKa = 4.81 LLALEE26 pKa = 5.32 DD27 pKa = 4.97 DD28 pKa = 4.25 YY29 pKa = 12.11 APGVDD34 pKa = 3.91 EE35 pKa = 4.74 GAVFADD41 pKa = 3.71 AA42 pKa = 5.34
Molecular weight: 4.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.793
IPC2_protein 4.024
IPC_protein 3.859
Toseland 3.668
ProMoST 4.05
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.834
Rodwell 3.694
Grimsley 3.592
Solomon 3.821
Lehninger 3.783
Nozaki 4.012
DTASelect 4.19
Thurlkill 3.745
EMBOSS 3.834
Sillero 3.986
Patrickios 1.926
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.891
Protein with the highest isoelectric point:
>tr|A0A239VA44|A0A239VA44_9MICO Proline-specific permease ProY OS=Dermatophilus congolensis OX=1863 GN=proY PE=3 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 9.8 KK16 pKa = 9.13 HH17 pKa = 4.41 GFRR20 pKa = 11.84 SRR22 pKa = 11.84 MATRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILACRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.88 GRR40 pKa = 11.84 ASISAA45 pKa = 3.59
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.44
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.34
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.076
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.112
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2311
0
2311
781931
33
3782
338.4
36.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.114 ± 0.063
0.829 ± 0.016
5.499 ± 0.039
5.553 ± 0.057
2.91 ± 0.033
8.505 ± 0.058
2.639 ± 0.035
4.851 ± 0.047
2.664 ± 0.038
9.615 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.112 ± 0.021
2.721 ± 0.034
5.533 ± 0.043
3.114 ± 0.026
6.774 ± 0.05
5.728 ± 0.045
7.06 ± 0.081
8.392 ± 0.077
1.43 ± 0.024
1.956 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here