Chrysochromulina parva virophage Curly
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A499SAG3|A0A499SAG3_9VIRU S3H helicase with TVpol domain OS=Chrysochromulina parva virophage Curly OX=2420054 PE=4 SV=1
MM1 pKa = 7.12 PQNGLSMFDD10 pKa = 3.56 YY11 pKa = 10.55 YY12 pKa = 10.49 ISIGINPYY20 pKa = 10.39 SIGAIGFAGGGGGALAANSVSSSNIVDD47 pKa = 3.85 GTVEE51 pKa = 4.27 TVDD54 pKa = 4.08 LADD57 pKa = 5.66 GIITEE62 pKa = 4.7 AKK64 pKa = 9.82 FHH66 pKa = 6.5 PTLQTSLATFSQSLFNLEE84 pKa = 3.95 NPGFYY89 pKa = 9.38 GTLRR93 pKa = 11.84 IKK95 pKa = 10.75 NSNTSNVGATMTITVQSEE113 pKa = 5.02 YY114 pKa = 11.33 YY115 pKa = 10.0 DD116 pKa = 4.02 APSNTTDD123 pKa = 3.96 IIPRR127 pKa = 11.84 LTTTTLNRR135 pKa = 11.84 GNHH138 pKa = 5.67 IDD140 pKa = 3.24 IVIPISTRR148 pKa = 11.84 LTNKK152 pKa = 10.24 DD153 pKa = 3.5 SIFRR157 pKa = 11.84 MYY159 pKa = 9.51 YY160 pKa = 8.47 TLNFGALAEE169 pKa = 4.17 HH170 pKa = 6.83 SNVNVNVDD178 pKa = 4.33 EE179 pKa = 5.0 ISTNAIGDD187 pKa = 3.87 FVTFGTAKK195 pKa = 9.52 TYY197 pKa = 11.17 AVINGGSVEE206 pKa = 3.95 ISFEE210 pKa = 3.88 ISS212 pKa = 2.66
Molecular weight: 22.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.319
IPC2_protein 4.52
IPC_protein 4.418
Toseland 4.253
ProMoST 4.558
Dawson 4.393
Bjellqvist 4.546
Wikipedia 4.291
Rodwell 4.266
Grimsley 4.177
Solomon 4.393
Lehninger 4.342
Nozaki 4.507
DTASelect 4.698
Thurlkill 4.279
EMBOSS 4.304
Sillero 4.546
Patrickios 2.956
IPC_peptide 4.393
IPC2_peptide 4.533
IPC2.peptide.svr19 4.474
Protein with the highest isoelectric point:
>tr|A0A4P1LUE2|A0A4P1LUE2_9VIRU Uncharacterized protein OS=Chrysochromulina parva virophage Curly OX=2420054 PE=4 SV=1
MM1 pKa = 6.82 VAKK4 pKa = 10.73 FEE6 pKa = 4.44 DD7 pKa = 4.06 PKK9 pKa = 10.32 RR10 pKa = 11.84 TVHH13 pKa = 6.91 FGMKK17 pKa = 10.31 GSTTYY22 pKa = 10.57 IDD24 pKa = 4.77 DD25 pKa = 4.75 KK26 pKa = 11.68 DD27 pKa = 3.59 PARR30 pKa = 11.84 QKK32 pKa = 11.2 AYY34 pKa = 10.79 LDD36 pKa = 3.15 RR37 pKa = 11.84 HH38 pKa = 6.2 RR39 pKa = 11.84 EE40 pKa = 3.89 NEE42 pKa = 3.85 TWNNPTSPGSLARR55 pKa = 11.84 YY56 pKa = 8.92 ILWGDD61 pKa = 3.45 STSLKK66 pKa = 10.74 KK67 pKa = 10.72 NISAFKK73 pKa = 10.65 KK74 pKa = 10.37 RR75 pKa = 11.84 FNLL78 pKa = 3.73
Molecular weight: 9.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.023
IPC2_protein 9.238
IPC_protein 9.224
Toseland 10.087
ProMoST 9.663
Dawson 10.233
Bjellqvist 9.853
Wikipedia 10.365
Rodwell 10.789
Grimsley 10.292
Solomon 10.277
Lehninger 10.248
Nozaki 10.043
DTASelect 9.853
Thurlkill 10.101
EMBOSS 10.467
Sillero 10.145
Patrickios 10.57
IPC_peptide 10.277
IPC2_peptide 8.258
IPC2.peptide.svr19 8.277
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
0
19
6666
78
2106
350.8
39.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.696 ± 1.272
0.96 ± 0.378
5.866 ± 0.251
6.706 ± 0.532
3.705 ± 0.394
5.161 ± 0.451
1.53 ± 0.248
7.381 ± 0.493
7.306 ± 0.727
7.666 ± 0.402
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.475 ± 0.231
6.511 ± 0.829
5.746 ± 0.837
3.525 ± 0.393
4.275 ± 0.263
6.646 ± 0.341
6.751 ± 0.568
5.761 ± 0.211
0.525 ± 0.113
3.81 ± 0.482
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here