Methanobacterium virus PhiF1

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2W642|A0A5Q2W642_9VIRU Putative glycosyltransferase OS=Methanobacterium virus PhiF1 OX=2609788 PE=4 SV=1
MM1 pKa = 7.43VYY3 pKa = 10.92VMWLDD8 pKa = 3.27GRR10 pKa = 11.84PIGIRR15 pKa = 11.84DD16 pKa = 3.52DD17 pKa = 3.97EE18 pKa = 4.58FPGFYY23 pKa = 10.57EE24 pKa = 4.1EE25 pKa = 4.77TVDD28 pKa = 3.65EE29 pKa = 5.22ALIEE33 pKa = 4.37DD34 pKa = 4.29LMGVNPDD41 pKa = 2.93NMRR44 pKa = 11.84LVGYY48 pKa = 10.5FEE50 pKa = 5.42DD51 pKa = 4.96RR52 pKa = 11.84EE53 pKa = 4.23ALEE56 pKa = 4.16EE57 pKa = 4.07AVAPEE62 pKa = 4.72DD63 pKa = 3.68PDD65 pKa = 3.85MIIWDD70 pKa = 4.03GPDD73 pKa = 3.35FYY75 pKa = 11.02LYY77 pKa = 10.0WGVPDD82 pKa = 5.64PIVATWRR89 pKa = 11.84YY90 pKa = 9.4VLDD93 pKa = 4.26FEE95 pKa = 6.03VDD97 pKa = 3.63YY98 pKa = 11.37QDD100 pKa = 4.81YY101 pKa = 10.53EE102 pKa = 4.41VAQALSDD109 pKa = 3.48FRR111 pKa = 11.84DD112 pKa = 3.55YY113 pKa = 11.68VDD115 pKa = 4.23YY116 pKa = 11.36LEE118 pKa = 4.31QPGVISFEE126 pKa = 4.22EE127 pKa = 4.29EE128 pKa = 3.83TVLRR132 pKa = 11.84NLRR135 pKa = 11.84DD136 pKa = 3.56QLIDD140 pKa = 3.23SHH142 pKa = 5.52THH144 pKa = 4.66MEE146 pKa = 3.89VDD148 pKa = 3.71EE149 pKa = 4.2VLEE152 pKa = 3.91ALIDD156 pKa = 3.83RR157 pKa = 11.84LEE159 pKa = 5.02DD160 pKa = 3.13ICGDD164 pKa = 3.33RR165 pKa = 11.84LYY167 pKa = 10.61WVIDD171 pKa = 3.65PLCNGEE177 pKa = 6.26LKK179 pKa = 8.85TQWHH183 pKa = 6.2VDD185 pKa = 3.56LEE187 pKa = 4.36QEE189 pKa = 4.16LLYY192 pKa = 10.78KK193 pKa = 10.58VEE195 pKa = 4.26GLNN198 pKa = 4.06

Molecular weight: 23.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2W4S8|A0A5Q2W4S8_9VIRU Uncharacterized protein OS=Methanobacterium virus PhiF1 OX=2609788 PE=4 SV=1
MM1 pKa = 7.69LEE3 pKa = 3.97LAVNFAQAEE12 pKa = 4.25KK13 pKa = 10.34QGRR16 pKa = 11.84IVLANSYY23 pKa = 11.18AEE25 pKa = 4.38AEE27 pKa = 4.21FLEE30 pKa = 4.44RR31 pKa = 11.84TLPEE35 pKa = 3.93KK36 pKa = 10.3AAEE39 pKa = 3.95MLAAYY44 pKa = 8.78IQYY47 pKa = 10.3HH48 pKa = 5.99ILHH51 pKa = 6.38NQTKK55 pKa = 9.63GAPLYY60 pKa = 10.67EE61 pKa = 4.53SGNLYY66 pKa = 10.51NSVYY70 pKa = 9.99IDD72 pKa = 3.27QVGANDD78 pKa = 3.74YY79 pKa = 9.93MVGVYY84 pKa = 10.6ALDD87 pKa = 3.59EE88 pKa = 4.49KK89 pKa = 11.16GRR91 pKa = 11.84DD92 pKa = 3.44YY93 pKa = 11.41AIVLEE98 pKa = 4.23YY99 pKa = 11.2GLGNLKK105 pKa = 10.43GSYY108 pKa = 10.11PFFRR112 pKa = 11.84PALQQFFFDD121 pKa = 3.78VDD123 pKa = 4.13NITSLLTNSRR133 pKa = 11.84SPALPPLKK141 pKa = 10.56DD142 pKa = 2.99RR143 pKa = 11.84ASPIRR148 pKa = 11.84RR149 pKa = 11.84AAAYY153 pKa = 9.38KK154 pKa = 9.98GANKK158 pKa = 9.93RR159 pKa = 11.84RR160 pKa = 11.84MKK162 pKa = 10.28KK163 pKa = 9.38ISRR166 pKa = 11.84IKK168 pKa = 8.91GGYY171 pKa = 7.66YY172 pKa = 8.52RR173 pKa = 11.84WHH175 pKa = 6.23GRR177 pKa = 11.84RR178 pKa = 11.84EE179 pKa = 3.94EE180 pKa = 3.84FRR182 pKa = 11.84LYY184 pKa = 9.66LTSKK188 pKa = 9.94FGGRR192 pKa = 11.84FGTRR196 pKa = 11.84RR197 pKa = 11.84PEE199 pKa = 3.95AMPMGTKK206 pKa = 9.88RR207 pKa = 11.84QAYY210 pKa = 9.13RR211 pKa = 11.84LAKK214 pKa = 9.93GRR216 pKa = 11.84AASGRR221 pKa = 11.84PKK223 pKa = 10.03TISKK227 pKa = 9.82KK228 pKa = 10.17RR229 pKa = 11.84PEE231 pKa = 4.34GYY233 pKa = 9.79LRR235 pKa = 11.84HH236 pKa = 6.25KK237 pKa = 10.75RR238 pKa = 11.84RR239 pKa = 11.84DD240 pKa = 3.23

Molecular weight: 27.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

21428

76

1310

281.9

31.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.805 ± 0.383

1.265 ± 0.159

6.613 ± 0.278

7.07 ± 0.255

3.892 ± 0.212

7.705 ± 0.328

1.652 ± 0.137

7.187 ± 0.275

5.432 ± 0.305

7.938 ± 0.182

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.627 ± 0.169

3.985 ± 0.224

4.732 ± 0.204

2.632 ± 0.217

5.843 ± 0.277

6.258 ± 0.263

6.249 ± 0.573

6.818 ± 0.397

1.213 ± 0.107

5.082 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski