Amino acid dipepetide frequency for Methanobacterium virus PhiF1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.76AlaAla: 4.76 ± 0.79
0.653AlaCys: 0.653 ± 0.134
2.8AlaAsp: 2.8 ± 0.331
4.2AlaGlu: 4.2 ± 0.613
1.493AlaPhe: 1.493 ± 0.353
4.9AlaGly: 4.9 ± 0.819
0.7AlaHis: 0.7 ± 0.179
3.687AlaIle: 3.687 ± 0.486
3.92AlaLys: 3.92 ± 0.442
4.434AlaLeu: 4.434 ± 0.55
1.54AlaMet: 1.54 ± 0.324
2.52AlaAsn: 2.52 ± 0.295
2.193AlaPro: 2.193 ± 0.309
1.82AlaGln: 1.82 ± 0.334
3.22AlaArg: 3.22 ± 0.397
4.247AlaSer: 4.247 ± 0.502
4.154AlaThr: 4.154 ± 0.466
3.314AlaVal: 3.314 ± 0.647
0.7AlaTrp: 0.7 ± 0.182
2.8AlaTyr: 2.8 ± 0.409
0.0AlaXaa: 0.0 ± 0.0
Cys
0.56CysAla: 0.56 ± 0.157
0.28CysCys: 0.28 ± 0.105
1.073CysAsp: 1.073 ± 0.271
0.84CysGlu: 0.84 ± 0.162
0.373CysPhe: 0.373 ± 0.148
1.633CysGly: 1.633 ± 0.484
0.28CysHis: 0.28 ± 0.113
0.607CysIle: 0.607 ± 0.173
0.42CysLys: 0.42 ± 0.188
0.84CysLeu: 0.84 ± 0.187
0.327CysMet: 0.327 ± 0.149
0.327CysAsn: 0.327 ± 0.121
0.607CysPro: 0.607 ± 0.18
0.373CysGln: 0.373 ± 0.116
1.027CysArg: 1.027 ± 0.245
0.513CysSer: 0.513 ± 0.201
0.513CysThr: 0.513 ± 0.17
1.12CysVal: 1.12 ± 0.266
0.233CysTrp: 0.233 ± 0.107
0.7CysTyr: 0.7 ± 0.181
0.0CysXaa: 0.0 ± 0.0
Asp
3.407AspAla: 3.407 ± 0.327
0.7AspCys: 0.7 ± 0.186
4.2AspAsp: 4.2 ± 0.565
5.554AspGlu: 5.554 ± 0.64
2.567AspPhe: 2.567 ± 0.316
5.74AspGly: 5.74 ± 0.676
1.12AspHis: 1.12 ± 0.217
5.087AspIle: 5.087 ± 0.644
2.66AspLys: 2.66 ± 0.232
5.6AspLeu: 5.6 ± 0.577
1.68AspMet: 1.68 ± 0.214
3.08AspAsn: 3.08 ± 0.394
3.22AspPro: 3.22 ± 0.373
1.26AspGln: 1.26 ± 0.281
2.614AspArg: 2.614 ± 0.386
4.154AspSer: 4.154 ± 0.449
3.594AspThr: 3.594 ± 0.592
5.647AspVal: 5.647 ± 0.744
0.887AspTrp: 0.887 ± 0.214
3.36AspTyr: 3.36 ± 0.417
0.0AspXaa: 0.0 ± 0.0
Glu
5.927GluAla: 5.927 ± 0.698
0.98GluCys: 0.98 ± 0.215
4.714GluAsp: 4.714 ± 0.498
5.88GluGlu: 5.88 ± 0.732
3.594GluPhe: 3.594 ± 0.401
4.294GluGly: 4.294 ± 0.566
1.073GluHis: 1.073 ± 0.243
4.06GluIle: 4.06 ± 0.485
3.687GluLys: 3.687 ± 0.579
5.927GluLeu: 5.927 ± 0.765
1.773GluMet: 1.773 ± 0.352
2.52GluAsn: 2.52 ± 0.279
2.147GluPro: 2.147 ± 0.295
1.54GluGln: 1.54 ± 0.202
3.5GluArg: 3.5 ± 0.504
4.387GluSer: 4.387 ± 0.526
3.127GluThr: 3.127 ± 0.386
5.414GluVal: 5.414 ± 0.674
1.307GluTrp: 1.307 ± 0.21
4.854GluTyr: 4.854 ± 0.41
0.0GluXaa: 0.0 ± 0.0
Phe
2.24PheAla: 2.24 ± 0.349
0.747PheCys: 0.747 ± 0.216
3.127PheAsp: 3.127 ± 0.41
2.707PheGlu: 2.707 ± 0.422
1.447PhePhe: 1.447 ± 0.251
2.287PheGly: 2.287 ± 0.403
0.607PheHis: 0.607 ± 0.157
2.754PheIle: 2.754 ± 0.413
2.1PheLys: 2.1 ± 0.362
3.267PheLeu: 3.267 ± 0.416
1.073PheMet: 1.073 ± 0.239
1.493PheAsn: 1.493 ± 0.33
2.24PhePro: 2.24 ± 0.322
1.073PheGln: 1.073 ± 0.176
2.94PheArg: 2.94 ± 0.532
2.193PheSer: 2.193 ± 0.264
2.754PheThr: 2.754 ± 0.343
2.007PheVal: 2.007 ± 0.402
0.373PheTrp: 0.373 ± 0.147
1.493PheTyr: 1.493 ± 0.325
0.0PheXaa: 0.0 ± 0.0
Gly
4.434GlyAla: 4.434 ± 0.705
1.307GlyCys: 1.307 ± 0.269
6.16GlyAsp: 6.16 ± 0.717
5.087GlyGlu: 5.087 ± 0.456
3.78GlyPhe: 3.78 ± 0.556
6.44GlyGly: 6.44 ± 0.769
0.98GlyHis: 0.98 ± 0.24
3.78GlyIle: 3.78 ± 0.402
3.314GlyLys: 3.314 ± 0.645
6.487GlyLeu: 6.487 ± 0.754
1.867GlyMet: 1.867 ± 0.327
2.287GlyAsn: 2.287 ± 0.264
2.053GlyPro: 2.053 ± 0.254
1.633GlyGln: 1.633 ± 0.241
4.854GlyArg: 4.854 ± 0.602
4.014GlySer: 4.014 ± 0.509
5.134GlyThr: 5.134 ± 0.718
8.494GlyVal: 8.494 ± 0.858
0.747GlyTrp: 0.747 ± 0.193
4.2GlyTyr: 4.2 ± 0.458
0.0GlyXaa: 0.0 ± 0.0
His
1.027HisAla: 1.027 ± 0.23
0.233HisCys: 0.233 ± 0.122
0.933HisAsp: 0.933 ± 0.196
0.98HisGlu: 0.98 ± 0.234
0.607HisPhe: 0.607 ± 0.18
1.12HisGly: 1.12 ± 0.247
0.467HisHis: 0.467 ± 0.157
1.26HisIle: 1.26 ± 0.247
0.84HisLys: 0.84 ± 0.209
1.773HisLeu: 1.773 ± 0.299
0.28HisMet: 0.28 ± 0.1
0.653HisAsn: 0.653 ± 0.179
1.307HisPro: 1.307 ± 0.237
0.28HisGln: 0.28 ± 0.12
1.213HisArg: 1.213 ± 0.274
0.98HisSer: 0.98 ± 0.2
1.027HisThr: 1.027 ± 0.247
0.887HisVal: 0.887 ± 0.18
0.14HisTrp: 0.14 ± 0.081
0.513HisTyr: 0.513 ± 0.166
0.0HisXaa: 0.0 ± 0.0
Ile
3.267IleAla: 3.267 ± 0.695
0.793IleCys: 0.793 ± 0.16
5.087IleAsp: 5.087 ± 0.498
5.367IleGlu: 5.367 ± 0.43
2.38IlePhe: 2.38 ± 0.297
4.714IleGly: 4.714 ± 0.647
1.493IleHis: 1.493 ± 0.297
5.787IleIle: 5.787 ± 0.54
4.06IleLys: 4.06 ± 0.575
5.18IleLeu: 5.18 ± 0.563
1.727IleMet: 1.727 ± 0.325
3.314IleAsn: 3.314 ± 0.437
3.78IlePro: 3.78 ± 0.386
1.913IleGln: 1.913 ± 0.328
4.34IleArg: 4.34 ± 0.56
3.734IleSer: 3.734 ± 0.466
6.02IleThr: 6.02 ± 0.882
4.34IleVal: 4.34 ± 0.43
0.7IleTrp: 0.7 ± 0.181
3.827IleTyr: 3.827 ± 0.448
0.0IleXaa: 0.0 ± 0.0
Lys
3.5LysAla: 3.5 ± 0.519
0.84LysCys: 0.84 ± 0.204
3.827LysAsp: 3.827 ± 0.524
3.734LysGlu: 3.734 ± 0.711
1.633LysPhe: 1.633 ± 0.249
3.22LysGly: 3.22 ± 0.41
0.747LysHis: 0.747 ± 0.203
4.154LysIle: 4.154 ± 0.415
3.734LysLys: 3.734 ± 0.553
3.92LysLeu: 3.92 ± 0.448
1.167LysMet: 1.167 ± 0.254
1.913LysAsn: 1.913 ± 0.342
2.754LysPro: 2.754 ± 0.294
1.307LysGln: 1.307 ± 0.236
3.687LysArg: 3.687 ± 0.532
2.987LysSer: 2.987 ± 0.435
3.36LysThr: 3.36 ± 0.585
4.247LysVal: 4.247 ± 0.464
0.98LysTrp: 0.98 ± 0.214
2.614LysTyr: 2.614 ± 0.38
0.0LysXaa: 0.0 ± 0.0
Leu
3.734LeuAla: 3.734 ± 0.532
1.073LeuCys: 1.073 ± 0.236
4.154LeuAsp: 4.154 ± 0.404
6.02LeuGlu: 6.02 ± 0.644
2.894LeuPhe: 2.894 ± 0.305
5.694LeuGly: 5.694 ± 0.746
1.307LeuHis: 1.307 ± 0.293
5.787LeuIle: 5.787 ± 0.65
5.227LeuLys: 5.227 ± 0.512
4.9LeuLeu: 4.9 ± 0.519
2.474LeuMet: 2.474 ± 0.427
3.594LeuAsn: 3.594 ± 0.41
3.594LeuPro: 3.594 ± 0.355
2.707LeuGln: 2.707 ± 0.332
4.434LeuArg: 4.434 ± 0.475
6.347LeuSer: 6.347 ± 0.488
5.554LeuThr: 5.554 ± 0.616
3.687LeuVal: 3.687 ± 0.411
1.26LeuTrp: 1.26 ± 0.3
4.947LeuTyr: 4.947 ± 0.461
0.0LeuXaa: 0.0 ± 0.0
Met
1.773MetAla: 1.773 ± 0.376
0.233MetCys: 0.233 ± 0.101
1.867MetAsp: 1.867 ± 0.304
1.867MetGlu: 1.867 ± 0.347
1.073MetPhe: 1.073 ± 0.232
1.587MetGly: 1.587 ± 0.24
0.28MetHis: 0.28 ± 0.082
2.053MetIle: 2.053 ± 0.354
1.913MetLys: 1.913 ± 0.291
1.68MetLeu: 1.68 ± 0.317
1.167MetMet: 1.167 ± 0.265
0.84MetAsn: 0.84 ± 0.193
0.84MetPro: 0.84 ± 0.204
0.42MetGln: 0.42 ± 0.143
2.1MetArg: 2.1 ± 0.344
1.727MetSer: 1.727 ± 0.259
1.493MetThr: 1.493 ± 0.269
1.96MetVal: 1.96 ± 0.33
0.467MetTrp: 0.467 ± 0.149
0.933MetTyr: 0.933 ± 0.292
0.0MetXaa: 0.0 ± 0.0
Asn
1.867AsnAla: 1.867 ± 0.3
0.327AsnCys: 0.327 ± 0.141
1.96AsnAsp: 1.96 ± 0.293
2.193AsnGlu: 2.193 ± 0.329
1.26AsnPhe: 1.26 ± 0.238
3.5AsnGly: 3.5 ± 0.397
0.98AsnHis: 0.98 ± 0.219
3.827AsnIle: 3.827 ± 0.457
2.24AsnLys: 2.24 ± 0.234
4.014AsnLeu: 4.014 ± 0.459
1.12AsnMet: 1.12 ± 0.288
1.82AsnAsn: 1.82 ± 0.361
3.36AsnPro: 3.36 ± 0.428
1.4AsnGln: 1.4 ± 0.28
2.053AsnArg: 2.053 ± 0.345
2.287AsnSer: 2.287 ± 0.285
2.24AsnThr: 2.24 ± 0.471
2.147AsnVal: 2.147 ± 0.303
0.233AsnTrp: 0.233 ± 0.112
1.027AsnTyr: 1.027 ± 0.199
0.0AsnXaa: 0.0 ± 0.0
Pro
2.8ProAla: 2.8 ± 0.38
0.56ProCys: 0.56 ± 0.174
3.5ProAsp: 3.5 ± 0.316
3.454ProGlu: 3.454 ± 0.341
1.68ProPhe: 1.68 ± 0.291
3.547ProGly: 3.547 ± 0.387
0.653ProHis: 0.653 ± 0.196
3.127ProIle: 3.127 ± 0.52
2.614ProLys: 2.614 ± 0.341
3.874ProLeu: 3.874 ± 0.324
0.793ProMet: 0.793 ± 0.197
1.96ProAsn: 1.96 ± 0.394
2.287ProPro: 2.287 ± 0.289
1.307ProGln: 1.307 ± 0.309
2.38ProArg: 2.38 ± 0.367
3.64ProSer: 3.64 ± 0.523
2.427ProThr: 2.427 ± 0.498
3.267ProVal: 3.267 ± 0.427
0.467ProTrp: 0.467 ± 0.133
2.987ProTyr: 2.987 ± 0.397
0.0ProXaa: 0.0 ± 0.0
Gln
1.727GlnAla: 1.727 ± 0.352
0.14GlnCys: 0.14 ± 0.081
1.4GlnAsp: 1.4 ± 0.251
0.933GlnGlu: 0.933 ± 0.179
1.26GlnPhe: 1.26 ± 0.195
2.053GlnGly: 2.053 ± 0.407
0.42GlnHis: 0.42 ± 0.173
2.24GlnIle: 2.24 ± 0.261
1.54GlnLys: 1.54 ± 0.358
2.567GlnLeu: 2.567 ± 0.507
0.84GlnMet: 0.84 ± 0.258
1.167GlnAsn: 1.167 ± 0.207
1.213GlnPro: 1.213 ± 0.24
0.84GlnGln: 0.84 ± 0.218
1.12GlnArg: 1.12 ± 0.33
1.633GlnSer: 1.633 ± 0.253
1.867GlnThr: 1.867 ± 0.323
1.727GlnVal: 1.727 ± 0.29
0.233GlnTrp: 0.233 ± 0.114
1.4GlnTyr: 1.4 ± 0.287
0.0GlnXaa: 0.0 ± 0.0
Arg
3.36ArgAla: 3.36 ± 0.521
0.7ArgCys: 0.7 ± 0.197
3.594ArgAsp: 3.594 ± 0.471
3.827ArgGlu: 3.827 ± 0.461
2.847ArgPhe: 2.847 ± 0.408
4.76ArgGly: 4.76 ± 0.538
0.793ArgHis: 0.793 ± 0.16
4.247ArgIle: 4.247 ± 0.405
2.894ArgLys: 2.894 ± 0.395
5.507ArgLeu: 5.507 ± 0.567
1.773ArgMet: 1.773 ± 0.334
1.867ArgAsn: 1.867 ± 0.324
2.193ArgPro: 2.193 ± 0.389
1.68ArgGln: 1.68 ± 0.321
4.854ArgArg: 4.854 ± 0.555
2.614ArgSer: 2.614 ± 0.365
2.193ArgThr: 2.193 ± 0.321
5.414ArgVal: 5.414 ± 0.7
0.653ArgTrp: 0.653 ± 0.213
2.66ArgTyr: 2.66 ± 0.421
0.0ArgXaa: 0.0 ± 0.0
Ser
3.034SerAla: 3.034 ± 0.571
0.933SerCys: 0.933 ± 0.191
3.734SerAsp: 3.734 ± 0.451
3.874SerGlu: 3.874 ± 0.494
2.52SerPhe: 2.52 ± 0.345
6.347SerGly: 6.347 ± 0.625
1.073SerHis: 1.073 ± 0.242
4.247SerIle: 4.247 ± 0.542
2.987SerLys: 2.987 ± 0.384
4.48SerLeu: 4.48 ± 0.463
1.447SerMet: 1.447 ± 0.281
2.52SerAsn: 2.52 ± 0.393
3.687SerPro: 3.687 ± 0.585
2.053SerGln: 2.053 ± 0.417
3.407SerArg: 3.407 ± 0.518
4.387SerSer: 4.387 ± 0.648
3.78SerThr: 3.78 ± 0.508
3.36SerVal: 3.36 ± 0.418
0.84SerTrp: 0.84 ± 0.188
3.874SerTyr: 3.874 ± 0.46
0.0SerXaa: 0.0 ± 0.0
Thr
3.827ThrAla: 3.827 ± 0.609
0.56ThrCys: 0.56 ± 0.182
3.734ThrAsp: 3.734 ± 0.609
3.454ThrGlu: 3.454 ± 0.609
2.474ThrPhe: 2.474 ± 0.395
5.04ThrGly: 5.04 ± 0.611
1.073ThrHis: 1.073 ± 0.216
6.16ThrIle: 6.16 ± 0.738
2.94ThrLys: 2.94 ± 0.388
5.367ThrLeu: 5.367 ± 0.525
1.633ThrMet: 1.633 ± 0.241
2.24ThrAsn: 2.24 ± 0.361
4.714ThrPro: 4.714 ± 0.639
1.633ThrGln: 1.633 ± 0.345
2.66ThrArg: 2.66 ± 0.346
4.154ThrSer: 4.154 ± 0.448
3.407ThrThr: 3.407 ± 1.145
3.92ThrVal: 3.92 ± 0.381
0.42ThrTrp: 0.42 ± 0.128
3.08ThrTyr: 3.08 ± 0.459
0.0ThrXaa: 0.0 ± 0.0
Val
3.594ValAla: 3.594 ± 0.487
0.793ValCys: 0.793 ± 0.251
5.414ValAsp: 5.414 ± 0.654
5.787ValGlu: 5.787 ± 0.595
3.127ValPhe: 3.127 ± 0.438
5.087ValGly: 5.087 ± 0.615
0.887ValHis: 0.887 ± 0.206
4.48ValIle: 4.48 ± 0.47
4.294ValLys: 4.294 ± 0.472
4.34ValLeu: 4.34 ± 0.627
1.913ValMet: 1.913 ± 0.364
2.894ValAsn: 2.894 ± 0.36
2.24ValPro: 2.24 ± 0.347
1.447ValGln: 1.447 ± 0.277
4.014ValArg: 4.014 ± 0.553
5.32ValSer: 5.32 ± 0.653
5.647ValThr: 5.647 ± 0.601
3.034ValVal: 3.034 ± 0.488
0.887ValTrp: 0.887 ± 0.254
2.987ValTyr: 2.987 ± 0.41
0.0ValXaa: 0.0 ± 0.0
Trp
0.653TrpAla: 0.653 ± 0.138
0.233TrpCys: 0.233 ± 0.133
1.073TrpAsp: 1.073 ± 0.204
0.747TrpGlu: 0.747 ± 0.139
0.327TrpPhe: 0.327 ± 0.091
1.073TrpGly: 1.073 ± 0.251
0.28TrpHis: 0.28 ± 0.131
0.653TrpIle: 0.653 ± 0.177
0.653TrpLys: 0.653 ± 0.161
1.167TrpLeu: 1.167 ± 0.249
0.793TrpMet: 0.793 ± 0.202
0.327TrpAsn: 0.327 ± 0.124
0.093TrpPro: 0.093 ± 0.058
0.187TrpGln: 0.187 ± 0.097
1.073TrpArg: 1.073 ± 0.242
0.7TrpSer: 0.7 ± 0.167
0.607TrpThr: 0.607 ± 0.163
1.073TrpVal: 1.073 ± 0.212
0.093TrpTrp: 0.093 ± 0.065
0.327TrpTyr: 0.327 ± 0.115
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.567TyrAla: 2.567 ± 0.384
0.56TyrCys: 0.56 ± 0.195
3.78TyrAsp: 3.78 ± 0.38
4.2TyrGlu: 4.2 ± 0.562
1.587TyrPhe: 1.587 ± 0.265
4.014TyrGly: 4.014 ± 0.414
1.307TyrHis: 1.307 ± 0.25
3.874TyrIle: 3.874 ± 0.571
2.287TyrLys: 2.287 ± 0.343
4.06TyrLeu: 4.06 ± 0.437
0.84TyrMet: 0.84 ± 0.15
2.52TyrAsn: 2.52 ± 0.338
2.8TyrPro: 2.8 ± 0.373
1.447TyrGln: 1.447 ± 0.198
2.94TyrArg: 2.94 ± 0.355
2.567TyrSer: 2.567 ± 0.384
3.594TyrThr: 3.594 ± 0.46
3.127TyrVal: 3.127 ± 0.322
0.513TyrTrp: 0.513 ± 0.155
2.24TyrTyr: 2.24 ± 0.385
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (21428 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski