Olsenella profusa F0195
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2650 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U2TT32|U2TT32_9ACTN ABC transporter transmembrane region domain protein OS=Olsenella profusa F0195 OX=1125712 GN=HMPREF1316_1668 PE=4 SV=1
MM1 pKa = 7.69 PWPEE5 pKa = 3.71 RR6 pKa = 11.84 ALVFLVTLVLALGVGAGVRR25 pKa = 11.84 RR26 pKa = 11.84 CLAQPTDD33 pKa = 3.74 YY34 pKa = 10.81 GPDD37 pKa = 3.33 VITPTDD43 pKa = 3.53 SVTDD47 pKa = 3.77 GTAPDD52 pKa = 3.97 DD53 pKa = 3.64 QGAATDD59 pKa = 3.88 AASNAPTSLTAEE71 pKa = 4.4 ADD73 pKa = 3.21 GGALVLSGDD82 pKa = 3.61 VGSRR86 pKa = 11.84 TVDD89 pKa = 3.34 DD90 pKa = 4.75 LSAVVQEE97 pKa = 5.2 LDD99 pKa = 3.15 ALQDD103 pKa = 3.36 GGIQAGVAVTSLDD116 pKa = 3.32 GSITLTYY123 pKa = 10.5 QQDD126 pKa = 3.32 QSFYY130 pKa = 10.35 AASTIKK136 pKa = 10.6 APYY139 pKa = 7.69 VCAVLEE145 pKa = 4.15 EE146 pKa = 4.7 DD147 pKa = 4.67 LPAGTTSMASLRR159 pKa = 11.84 DD160 pKa = 3.64 AMSSMLLYY168 pKa = 10.52 SDD170 pKa = 3.66 NDD172 pKa = 3.39 SYY174 pKa = 11.69 RR175 pKa = 11.84 QLRR178 pKa = 11.84 DD179 pKa = 3.07 SYY181 pKa = 11.59 GDD183 pKa = 3.77 DD184 pKa = 4.59 VFLTWLQRR192 pKa = 11.84 HH193 pKa = 6.15 DD194 pKa = 4.01 VTAGTYY200 pKa = 10.71 GSLSDD205 pKa = 4.12 YY206 pKa = 11.36 ARR208 pKa = 11.84 DD209 pKa = 3.79 HH210 pKa = 6.77 YY211 pKa = 11.07 PFSTPEE217 pKa = 3.76 QLTQMWQAVWAFVSGDD233 pKa = 3.61 AQGASYY239 pKa = 10.96 LKK241 pKa = 10.6 DD242 pKa = 3.08 LLARR246 pKa = 11.84 RR247 pKa = 11.84 EE248 pKa = 3.96 EE249 pKa = 4.3 SPIANALGPQTEE261 pKa = 4.9 TYY263 pKa = 10.67 SKK265 pKa = 10.77 AGWYY269 pKa = 9.61 PEE271 pKa = 4.13 TDD273 pKa = 3.56 GSDD276 pKa = 3.21 AAPASNDD283 pKa = 3.19 AGVVIHH289 pKa = 6.69 GSASTSYY296 pKa = 10.14 VISVMSTAPSEE307 pKa = 4.08 LDD309 pKa = 3.16 RR310 pKa = 11.84 LEE312 pKa = 4.36 GLISAIDD319 pKa = 3.75 HH320 pKa = 6.83 LMDD323 pKa = 5.46 ADD325 pKa = 4.15 GAA327 pKa = 4.23
Molecular weight: 34.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.719
IPC2_protein 3.808
IPC_protein 3.846
Toseland 3.605
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.668
Grimsley 3.516
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.253
Thurlkill 3.668
EMBOSS 3.808
Sillero 3.973
Patrickios 1.914
IPC_peptide 3.834
IPC2_peptide 3.948
IPC2.peptide.svr19 3.865
Protein with the highest isoelectric point:
>tr|U2TID9|U2TID9_9ACTN Uncharacterized protein OS=Olsenella profusa F0195 OX=1125712 GN=HMPREF1316_0657 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.34 QPNKK9 pKa = 8.62 RR10 pKa = 11.84 KK11 pKa = 9.56 RR12 pKa = 11.84 AKK14 pKa = 8.76 THH16 pKa = 5.23 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MASKK25 pKa = 10.41 AGRR28 pKa = 11.84 AVLSRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.16 GRR39 pKa = 11.84 KK40 pKa = 8.12 QLTVV44 pKa = 2.97
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2650
0
2650
795533
33
1732
300.2
32.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.992 ± 0.061
1.578 ± 0.019
6.457 ± 0.042
6.064 ± 0.049
3.287 ± 0.032
8.842 ± 0.049
2.239 ± 0.025
4.663 ± 0.038
2.898 ± 0.036
9.526 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.774 ± 0.019
2.512 ± 0.031
4.365 ± 0.037
2.926 ± 0.028
7.244 ± 0.059
5.989 ± 0.042
5.523 ± 0.034
8.177 ± 0.045
1.184 ± 0.02
2.762 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here