Phaethon lepturus (White-tailed tropicbird)
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9152 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A091UPN4|A0A091UPN4_PHALP Uncharacterized protein (Fragment) OS=Phaethon lepturus OX=97097 GN=N335_06667 PE=4 SV=1
LL1 pKa = 6.74 STQHH5 pKa = 5.64 EE6 pKa = 4.47 TSDD9 pKa = 4.53 DD10 pKa = 3.91 SLSDD14 pKa = 3.56 VPKK17 pKa = 10.89 NPEE20 pKa = 4.41 DD21 pKa = 4.83 LDD23 pKa = 4.06 GCDD26 pKa = 3.64 EE27 pKa = 4.42 VEE29 pKa = 4.57 SSAEE33 pKa = 3.92 VLPSSSKK40 pKa = 10.62 VSVIPHH46 pKa = 6.57 DD47 pKa = 3.95 LFYY50 pKa = 10.81 YY51 pKa = 8.96 PHH53 pKa = 6.67 YY54 pKa = 9.71 NVPISAVLNAYY65 pKa = 10.06 LEE67 pKa = 4.35 PCIEE71 pKa = 4.99 GYY73 pKa = 8.21 DD74 pKa = 3.71 TGNDD78 pKa = 3.12 NAASEE83 pKa = 4.48 TVTDD87 pKa = 3.77 VLHH90 pKa = 6.76 EE91 pKa = 4.74 KK92 pKa = 10.5 GLPEE96 pKa = 4.25 QNYY99 pKa = 8.41 TEE101 pKa = 4.73 DD102 pKa = 3.46 AAEE105 pKa = 4.17 TEE107 pKa = 4.6 LGNKK111 pKa = 9.94 LGVPPSEE118 pKa = 3.9 IDD120 pKa = 3.39 TEE122 pKa = 4.27 NGEE125 pKa = 4.32 EE126 pKa = 3.74 EE127 pKa = 3.95 AADD130 pKa = 4.03 IKK132 pKa = 11.22 CHH134 pKa = 5.87 MNFSDD139 pKa = 3.82 EE140 pKa = 4.55 KK141 pKa = 10.53 EE142 pKa = 4.25 VPLLVGEE149 pKa = 4.12 EE150 pKa = 4.31 LEE152 pKa = 4.32 IEE154 pKa = 4.15 EE155 pKa = 5.74 DD156 pKa = 3.34 GTKK159 pKa = 10.82 ANNHH163 pKa = 5.46 QDD165 pKa = 3.09 STIPQHH171 pKa = 6.68 PEE173 pKa = 3.22 VV174 pKa = 4.22
Molecular weight: 19.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.923
IPC_protein 3.884
Toseland 3.694
ProMoST 4.012
Dawson 3.834
Bjellqvist 3.999
Wikipedia 3.719
Rodwell 3.719
Grimsley 3.605
Solomon 3.834
Lehninger 3.783
Nozaki 3.948
DTASelect 4.101
Thurlkill 3.719
EMBOSS 3.745
Sillero 3.999
Patrickios 1.163
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.871
Protein with the highest isoelectric point:
>tr|A0A091TPW6|A0A091TPW6_PHALP Uncharacterized protein (Fragment) OS=Phaethon lepturus OX=97097 GN=N335_12016 PE=4 SV=1
KKK2 pKa = 10.12 RR3 pKa = 11.84 QGKKK7 pKa = 8.85 RR8 pKa = 11.84 QGKKK12 pKa = 8.73 RR13 pKa = 11.84 QGKKK17 pKa = 8.73 RR18 pKa = 11.84 QGKKK22 pKa = 8.73 RR23 pKa = 11.84 QGKKK27 pKa = 8.73 RR28 pKa = 11.84 QGKKK32 pKa = 8.73 RR33 pKa = 11.84 QGKKK37 pKa = 8.73 RR38 pKa = 11.84 QGKKK42 pKa = 8.73 RR43 pKa = 11.84 QGKKK47 pKa = 8.73 RR48 pKa = 11.84 QGKKK52 pKa = 8.73 RR53 pKa = 11.84 QGKKK57 pKa = 8.64 RR58 pKa = 11.84 QVFKKK63 pKa = 10.91 KK64 pKa = 10.27 LAQRR68 pKa = 11.84 V
Molecular weight: 7.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.359
IPC_protein 12.925
Toseland 13.1
ProMoST 13.583
Dawson 13.1
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.91
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.632
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.216
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9152
0
9152
3916303
33
5837
427.9
48.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.323 ± 0.023
2.23 ± 0.026
5.086 ± 0.02
7.244 ± 0.033
3.914 ± 0.019
5.812 ± 0.027
2.527 ± 0.014
5.03 ± 0.023
6.498 ± 0.03
9.698 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.269 ± 0.012
4.199 ± 0.018
5.231 ± 0.033
4.706 ± 0.026
5.185 ± 0.018
8.181 ± 0.044
5.403 ± 0.019
6.288 ± 0.025
1.187 ± 0.009
2.985 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here