Paraburkholderia sartisoli
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5332 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H4G7P0|A0A1H4G7P0_9BURK Cd(II)/Pb(II)-responsive transcriptional regulator OS=Paraburkholderia sartisoli OX=83784 GN=SAMN05192564_105368 PE=4 SV=1
MM1 pKa = 8.03 GYY3 pKa = 9.07 QQGLSGLAGASSDD16 pKa = 4.56 LDD18 pKa = 3.81 VIGNNIANANTVGFKK33 pKa = 10.29 QGSAQFADD41 pKa = 4.02 MYY43 pKa = 10.58 ANSVATAVNTQIGIGSRR60 pKa = 11.84 LASVQQQFSQGTINTTNQALDD81 pKa = 3.33 VAINGNGFFQMSNNGSLTYY100 pKa = 10.49 SRR102 pKa = 11.84 NGVFQLDD109 pKa = 3.58 KK110 pKa = 11.41 NGFIINADD118 pKa = 3.47 GLEE121 pKa = 4.0 LMGYY125 pKa = 9.03 AANANGIINSANTVPLKK142 pKa = 11.05 VPTANIPPTTTTTITAGLNLNAQDD166 pKa = 4.45 PLVLGTPTTTPGGTNTAGLTGTAAITSNVAGTNANTYY203 pKa = 10.12 LVTFTGPTTYY213 pKa = 9.71 TVSVNGGPASAPATYY228 pKa = 7.64 TTGTAITLGTGEE240 pKa = 4.53 TLTLAGATPANGDD253 pKa = 3.73 TFTVKK258 pKa = 9.1 PTQVPFNQTDD268 pKa = 3.34 NTTYY272 pKa = 10.61 SYY274 pKa = 7.4 PTSVPVYY281 pKa = 9.8 DD282 pKa = 4.29 SLGGAQQVDD291 pKa = 4.06 MYY293 pKa = 10.3 FVKK296 pKa = 9.91 TAAGSWDD303 pKa = 3.55 VYY305 pKa = 11.21 AGVSTGTASKK315 pKa = 10.43 IGTAKK320 pKa = 10.4 FDD322 pKa = 3.52 TSGNLVSTTDD332 pKa = 3.29 NAGNPTATPLAFNFSIPTTDD352 pKa = 4.1 GSATPQSLQLRR363 pKa = 11.84 IGGTTQFGSKK373 pKa = 10.36 DD374 pKa = 3.5 GTNSLDD380 pKa = 3.57 QNGFAAGTLTNFTIGQDD397 pKa = 3.33 GTLTGNYY404 pKa = 10.23 SNGQTAALGQIVLANFANQNGLVDD428 pKa = 5.02 LGNNQYY434 pKa = 11.02 GATAASGVAQISVPGSTNHH453 pKa = 5.88 GTLQGGAVEE462 pKa = 4.37 NSNVDD467 pKa = 3.69 LTSEE471 pKa = 4.14 LVNLITAQRR480 pKa = 11.84 NYY482 pKa = 9.6 QANAQTIKK490 pKa = 9.27 TQQAVDD496 pKa = 3.37 NTLINLL502 pKa = 4.1
Molecular weight: 50.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.812
IPC2_protein 4.05
IPC_protein 4.062
Toseland 3.821
ProMoST 4.253
Dawson 4.075
Bjellqvist 4.228
Wikipedia 4.05
Rodwell 3.884
Grimsley 3.732
Solomon 4.075
Lehninger 4.024
Nozaki 4.19
DTASelect 4.507
Thurlkill 3.897
EMBOSS 4.05
Sillero 4.19
Patrickios 1.163
IPC_peptide 4.062
IPC2_peptide 4.164
IPC2.peptide.svr19 4.055
Protein with the highest isoelectric point:
>tr|A0A1H3ZBF2|A0A1H3ZBF2_9BURK 2-polyprenyl-6-methoxyphenol hydroxylase OS=Paraburkholderia sartisoli OX=83784 GN=SAMN05192564_101667 PE=4 SV=1
MM1 pKa = 7.99 RR2 pKa = 11.84 ARR4 pKa = 11.84 TRR6 pKa = 11.84 IRR8 pKa = 11.84 LQTQTQTQTQTQTQTQTQTQTQTQVQVQVQVQVQVRR44 pKa = 11.84 MRR46 pKa = 11.84 MRR48 pKa = 11.84 VWVV51 pKa = 3.59
Molecular weight: 6.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.462
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.281
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.153
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5332
0
5332
1708161
26
3849
320.4
34.75
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.611 ± 0.042
0.924 ± 0.011
5.445 ± 0.025
5.049 ± 0.037
3.749 ± 0.022
8.252 ± 0.039
2.352 ± 0.017
4.786 ± 0.024
3.09 ± 0.029
10.122 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.421 ± 0.015
2.827 ± 0.026
5.075 ± 0.026
3.519 ± 0.025
6.892 ± 0.035
5.622 ± 0.032
5.608 ± 0.031
7.908 ± 0.023
1.354 ± 0.015
2.394 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here