Paraburkholderia sartisoli

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5332 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H4G7P0|A0A1H4G7P0_9BURK Cd(II)/Pb(II)-responsive transcriptional regulator OS=Paraburkholderia sartisoli OX=83784 GN=SAMN05192564_105368 PE=4 SV=1
MM1 pKa = 8.03GYY3 pKa = 9.07QQGLSGLAGASSDD16 pKa = 4.56LDD18 pKa = 3.81VIGNNIANANTVGFKK33 pKa = 10.29QGSAQFADD41 pKa = 4.02MYY43 pKa = 10.58ANSVATAVNTQIGIGSRR60 pKa = 11.84LASVQQQFSQGTINTTNQALDD81 pKa = 3.33VAINGNGFFQMSNNGSLTYY100 pKa = 10.49SRR102 pKa = 11.84NGVFQLDD109 pKa = 3.58KK110 pKa = 11.41NGFIINADD118 pKa = 3.47GLEE121 pKa = 4.0LMGYY125 pKa = 9.03AANANGIINSANTVPLKK142 pKa = 11.05VPTANIPPTTTTTITAGLNLNAQDD166 pKa = 4.45PLVLGTPTTTPGGTNTAGLTGTAAITSNVAGTNANTYY203 pKa = 10.12LVTFTGPTTYY213 pKa = 9.71TVSVNGGPASAPATYY228 pKa = 7.64TTGTAITLGTGEE240 pKa = 4.53TLTLAGATPANGDD253 pKa = 3.73TFTVKK258 pKa = 9.1PTQVPFNQTDD268 pKa = 3.34NTTYY272 pKa = 10.61SYY274 pKa = 7.4PTSVPVYY281 pKa = 9.8DD282 pKa = 4.29SLGGAQQVDD291 pKa = 4.06MYY293 pKa = 10.3FVKK296 pKa = 9.91TAAGSWDD303 pKa = 3.55VYY305 pKa = 11.21AGVSTGTASKK315 pKa = 10.43IGTAKK320 pKa = 10.4FDD322 pKa = 3.52TSGNLVSTTDD332 pKa = 3.29NAGNPTATPLAFNFSIPTTDD352 pKa = 4.1GSATPQSLQLRR363 pKa = 11.84IGGTTQFGSKK373 pKa = 10.36DD374 pKa = 3.5GTNSLDD380 pKa = 3.57QNGFAAGTLTNFTIGQDD397 pKa = 3.33GTLTGNYY404 pKa = 10.23SNGQTAALGQIVLANFANQNGLVDD428 pKa = 5.02LGNNQYY434 pKa = 11.02GATAASGVAQISVPGSTNHH453 pKa = 5.88GTLQGGAVEE462 pKa = 4.37NSNVDD467 pKa = 3.69LTSEE471 pKa = 4.14LVNLITAQRR480 pKa = 11.84NYY482 pKa = 9.6QANAQTIKK490 pKa = 9.27TQQAVDD496 pKa = 3.37NTLINLL502 pKa = 4.1

Molecular weight: 50.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H3ZBF2|A0A1H3ZBF2_9BURK 2-polyprenyl-6-methoxyphenol hydroxylase OS=Paraburkholderia sartisoli OX=83784 GN=SAMN05192564_101667 PE=4 SV=1
MM1 pKa = 7.99RR2 pKa = 11.84ARR4 pKa = 11.84TRR6 pKa = 11.84IRR8 pKa = 11.84LQTQTQTQTQTQTQTQTQTQTQTQVQVQVQVQVQVRR44 pKa = 11.84MRR46 pKa = 11.84MRR48 pKa = 11.84VWVV51 pKa = 3.59

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5332

0

5332

1708161

26

3849

320.4

34.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.611 ± 0.042

0.924 ± 0.011

5.445 ± 0.025

5.049 ± 0.037

3.749 ± 0.022

8.252 ± 0.039

2.352 ± 0.017

4.786 ± 0.024

3.09 ± 0.029

10.122 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.421 ± 0.015

2.827 ± 0.026

5.075 ± 0.026

3.519 ± 0.025

6.892 ± 0.035

5.622 ± 0.032

5.608 ± 0.031

7.908 ± 0.023

1.354 ± 0.015

2.394 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski