Sulfurospirillum cavolei
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1874 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D3WDG9|A0A2D3WDG9_9PROT Nitrogenase molybdenum-cofactor biosynthesis protein NifE OS=Sulfurospirillum cavolei OX=366522 GN=CFH80_01145 PE=3 SV=1
MM1 pKa = 7.46 SNAPEE6 pKa = 4.15 VIYY9 pKa = 10.9 SKK11 pKa = 10.02 STISLKK17 pKa = 10.65 SLPAKK22 pKa = 10.51 GEE24 pKa = 3.89 NLNLFLRR31 pKa = 11.84 PGDD34 pKa = 4.08 EE35 pKa = 4.68 LSLALDD41 pKa = 3.82 LSKK44 pKa = 11.41 AKK46 pKa = 10.29 FQVVGGDD53 pKa = 4.01 IIATLPSGGQLTFVSLGMMAFEE75 pKa = 4.83 NNAPVIKK82 pKa = 10.38 LPNGTFLALEE92 pKa = 4.36 QILDD96 pKa = 4.06 TITDD100 pKa = 3.57 VGQATKK106 pKa = 10.82 DD107 pKa = 3.37 AVLVSGNVSLEE118 pKa = 4.13 TQEE121 pKa = 4.32 QPIEE125 pKa = 4.15 HH126 pKa = 5.86 EE127 pKa = 4.51 QKK129 pKa = 11.08 AKK131 pKa = 9.36 TQDD134 pKa = 3.39 APVNDD139 pKa = 3.62 YY140 pKa = 10.52 NAYY143 pKa = 10.14 YY144 pKa = 10.55 VDD146 pKa = 3.91 PEE148 pKa = 4.29 LPKK151 pKa = 10.8 RR152 pKa = 11.84 LDD154 pKa = 3.61 DD155 pKa = 3.79 VASKK159 pKa = 11.11 DD160 pKa = 3.59 EE161 pKa = 4.27 SGKK164 pKa = 10.22 YY165 pKa = 9.25 LQEE168 pKa = 4.18 SLPEE172 pKa = 3.99 FTSNEE177 pKa = 4.05 TAKK180 pKa = 10.85 EE181 pKa = 3.89 DD182 pKa = 3.62 LTNKK186 pKa = 9.89 YY187 pKa = 10.31 SSSEE191 pKa = 4.08 EE192 pKa = 3.84 KK193 pKa = 10.37 TKK195 pKa = 11.48 DD196 pKa = 3.21 NVADD200 pKa = 3.69 VSAALSFDD208 pKa = 3.28 VGFYY212 pKa = 9.93 QIKK215 pKa = 10.22 GSEE218 pKa = 4.14 STADD222 pKa = 3.14 SVTSVLGGTGSALGNVSKK240 pKa = 10.81 LAAAQFEE247 pKa = 4.91 PEE249 pKa = 3.97 TLDD252 pKa = 3.54 YY253 pKa = 11.18 RR254 pKa = 11.84 NSAYY258 pKa = 9.75 STVITADD265 pKa = 2.89 NPAFVNATYY274 pKa = 8.72 LTKK277 pKa = 10.53 LVRR280 pKa = 11.84 LNVSQPIGFTITSIAINGLTSGGFEE305 pKa = 4.13 ILNSDD310 pKa = 4.59 FSSAGNASGGWTLSAGTGFSSTLSDD335 pKa = 3.13 GGEE338 pKa = 4.0 TIEE341 pKa = 5.3 FYY343 pKa = 10.54 IRR345 pKa = 11.84 YY346 pKa = 9.32 YY347 pKa = 9.81 PXNFDD352 pKa = 3.96 NLMQVALTSEE362 pKa = 4.67 FNMSNVPEE370 pKa = 4.26 EE371 pKa = 4.13 KK372 pKa = 10.18 QGDD375 pKa = 4.34 VIVPDD380 pKa = 3.79 LTKK383 pKa = 10.06 LTAYY387 pKa = 9.84 KK388 pKa = 10.49 DD389 pKa = 2.8 IGVIVKK395 pKa = 9.98 EE396 pKa = 3.96 VDD398 pKa = 3.41 SVSDD402 pKa = 3.33 YY403 pKa = 11.0 TYY405 pKa = 10.4 TGKK408 pKa = 10.15 HH409 pKa = 3.99 STGFVLDD416 pKa = 3.61 TTPNEE421 pKa = 4.27 NIVYY425 pKa = 9.12 TSKK428 pKa = 11.08 LDD430 pKa = 3.56 STVTGGLSNDD440 pKa = 3.18 TMYY443 pKa = 11.48 GSIGNDD449 pKa = 3.0 TLEE452 pKa = 4.48 GSNGNDD458 pKa = 3.39 TLSGGAGVNTLIGGSGIDD476 pKa = 3.2 TADD479 pKa = 3.18 YY480 pKa = 11.46 SFVTKK485 pKa = 10.88 YY486 pKa = 11.1 SDD488 pKa = 4.42 DD489 pKa = 3.72 FLFANDD495 pKa = 3.52 GHH497 pKa = 6.94 IDD499 pKa = 3.68 PVTGVAYY506 pKa = 10.35 ANDD509 pKa = 3.91 SKK511 pKa = 11.64 GVIVDD516 pKa = 4.25 LQAGAASGKK525 pKa = 7.32 TVYY528 pKa = 10.56 DD529 pKa = 3.39 ATSDD533 pKa = 3.66 VLTGEE538 pKa = 4.3 EE539 pKa = 4.11 SRR541 pKa = 11.84 TISDD545 pKa = 3.52 HH546 pKa = 6.69 LSGIEE551 pKa = 3.75 YY552 pKa = 10.66 VVGSKK557 pKa = 10.84 YY558 pKa = 10.93 DD559 pKa = 3.34 DD560 pKa = 4.28 TIRR563 pKa = 11.84 GDD565 pKa = 3.46 ANANKK570 pKa = 10.35 LSGGEE575 pKa = 4.2 GNDD578 pKa = 3.53 TLEE581 pKa = 4.68 GRR583 pKa = 11.84 DD584 pKa = 3.54 GKK586 pKa = 10.71 DD587 pKa = 2.77 WLEE590 pKa = 4.27 GGAGNDD596 pKa = 3.41 WLIGSTDD603 pKa = 3.78 DD604 pKa = 4.19 YY605 pKa = 11.13 MIDD608 pKa = 4.1 GGDD611 pKa = 3.63 NTDD614 pKa = 3.58 TADD617 pKa = 4.3 FSNNSNGLIITLNDD631 pKa = 3.45 SANGSLGNIGGTSATTIIKK650 pKa = 10.21 NVEE653 pKa = 4.06 NVAGTQYY660 pKa = 11.09 KK661 pKa = 10.69 DD662 pKa = 3.69 SISGDD667 pKa = 3.45 TADD670 pKa = 3.99 NLLIGGYY677 pKa = 8.44 DD678 pKa = 3.41 HH679 pKa = 7.47 SATAVTANDD688 pKa = 3.51 DD689 pKa = 4.28 TITGGSGADD698 pKa = 3.56 TIVGDD703 pKa = 3.79 MMIDD707 pKa = 3.54 TVIATPLYY715 pKa = 10.59 AGNDD719 pKa = 3.67 VLGGGSDD726 pKa = 3.51 NDD728 pKa = 4.3 TIYY731 pKa = 11.34 GDD733 pKa = 3.78 SAPILSNAEE742 pKa = 3.82 VEE744 pKa = 4.64 VAADD748 pKa = 3.34 QSVEE752 pKa = 4.22 YY753 pKa = 10.36 IRR755 pKa = 11.84 NTDD758 pKa = 3.85 DD759 pKa = 5.19 DD760 pKa = 4.25 STLATIYY767 pKa = 10.76 GGNDD771 pKa = 3.16 TLKK774 pKa = 11.09 GGGGDD779 pKa = 3.93 DD780 pKa = 3.89 YY781 pKa = 11.87 LNGGSGWDD789 pKa = 3.5 TVDD792 pKa = 3.7 YY793 pKa = 10.16 SASAAAVIVNLSSNYY808 pKa = 10.77 AMGEE812 pKa = 4.29 GYY814 pKa = 10.29 DD815 pKa = 3.27 QLFNIEE821 pKa = 4.82 NIVGSNSALGDD832 pKa = 3.76 TLIGNALANTIVGSTSGADD851 pKa = 3.46 TLSGMGGLDD860 pKa = 3.38 TLNGLGGKK868 pKa = 9.16 DD869 pKa = 3.01 WADD872 pKa = 3.31 YY873 pKa = 10.79 SYY875 pKa = 11.96 VSGTTGVNVDD885 pKa = 3.95 LTAGTAYY892 pKa = 10.62 VSATDD897 pKa = 3.85 GDD899 pKa = 4.01 TLIAIEE905 pKa = 4.11 NVIGSSKK912 pKa = 11.18 ADD914 pKa = 3.66 TLSGGSGNSVNTLMGGLGNDD934 pKa = 2.7 IFYY937 pKa = 10.54 GYY939 pKa = 11.14 GDD941 pKa = 4.31 GDD943 pKa = 4.29 LLDD946 pKa = 4.96 GGSGSDD952 pKa = 3.19 TADD955 pKa = 3.03 YY956 pKa = 9.17 MYY958 pKa = 10.8 AQGRR962 pKa = 11.84 IVVTLDD968 pKa = 3.22 GSNSDD973 pKa = 3.01 TLMNIEE979 pKa = 4.19 NVYY982 pKa = 10.26 GASDD986 pKa = 3.95 KK987 pKa = 10.63 DD988 pKa = 3.81 TLIGNSDD995 pKa = 3.54 ANILDD1000 pKa = 3.56 GRR1002 pKa = 11.84 ADD1004 pKa = 3.79 TDD1006 pKa = 3.78 TLSGMGGADD1015 pKa = 3.53 TLIGGAGDD1023 pKa = 3.53 DD1024 pKa = 4.13 TLIGGAGNDD1033 pKa = 4.2 TLWGGTDD1040 pKa = 3.24 ATHH1043 pKa = 8.06 DD1044 pKa = 3.98 SGIDD1048 pKa = 3.18 TADD1051 pKa = 3.4 YY1052 pKa = 10.62 SGSSSIYY1059 pKa = 10.32 ADD1061 pKa = 3.66 LANGTVNDD1069 pKa = 4.43 GLGGIDD1075 pKa = 3.34 TLAGIEE1081 pKa = 4.34 VILGSGSNDD1090 pKa = 3.15 VMIGSGNADD1099 pKa = 3.46 TLIGNGGNDD1108 pKa = 3.16 TFFASGGGDD1117 pKa = 3.04 SYY1119 pKa = 11.78 YY1120 pKa = 11.16 GGVFGSSSDD1129 pKa = 3.65 GNVKK1133 pKa = 10.46 DD1134 pKa = 3.74 RR1135 pKa = 11.84 LSYY1138 pKa = 11.09 ADD1140 pKa = 4.04 VYY1142 pKa = 11.34 HH1143 pKa = 7.34 DD1144 pKa = 4.61 VIDD1147 pKa = 4.27 SKK1149 pKa = 10.07 TVDD1152 pKa = 4.19 HH1153 pKa = 6.67 IVVDD1157 pKa = 5.01 LSHH1160 pKa = 6.92 VGTNATILDD1169 pKa = 3.76 ASNAVISTDD1178 pKa = 2.83 SLYY1181 pKa = 10.84 AIEE1184 pKa = 5.43 EE1185 pKa = 4.16 IVATDD1190 pKa = 3.64 GNDD1193 pKa = 3.52 TLRR1196 pKa = 11.84 GGAGSQQTFYY1206 pKa = 11.34 GGAGDD1211 pKa = 4.54 DD1212 pKa = 3.87 TLSGNTDD1219 pKa = 2.74 GDD1221 pKa = 4.11 YY1222 pKa = 11.5 LXTNLADD1229 pKa = 3.58 YY1230 pKa = 7.63 TAQSSNLVVNLASTSNNVYY1249 pKa = 10.65 LSGSSPTSSNSDD1261 pKa = 2.93 TLANINNIATGSGADD1276 pKa = 3.63 TIIGNGNDD1284 pKa = 3.53 NIVRR1288 pKa = 11.84 AGVGADD1294 pKa = 3.62 TLSGGAGDD1302 pKa = 3.63 DD1303 pKa = 3.69 TLYY1306 pKa = 11.52 GEE1308 pKa = 5.21 AGNDD1312 pKa = 3.54 TLSGGEE1318 pKa = 4.39 GNDD1321 pKa = 3.55 TLIGGMSTSVDD1332 pKa = 3.57 SGIDD1336 pKa = 3.28 TADD1339 pKa = 3.47 YY1340 pKa = 11.04 SATSYY1345 pKa = 11.43 YY1346 pKa = 10.69 GVTVDD1351 pKa = 4.72 LQNGNATGATIGTDD1365 pKa = 2.84 KK1366 pKa = 11.15 LYY1368 pKa = 11.02 GIEE1371 pKa = 4.11 NVLGSAQSDD1380 pKa = 3.92 TLIGASGTLNTLSGLGGNDD1399 pKa = 2.99 TLYY1402 pKa = 11.53 GNLEE1406 pKa = 3.76 GDD1408 pKa = 4.04 YY1409 pKa = 10.95 LDD1411 pKa = 5.23 GGSGTNTADD1420 pKa = 3.6 YY1421 pKa = 10.85 SSEE1424 pKa = 4.02 TASIVANLTTGRR1436 pKa = 11.84 AYY1438 pKa = 8.58 FTSSSSYY1445 pKa = 10.66 DD1446 pKa = 3.37 ALVSIQNVLSGSGNDD1461 pKa = 3.65 TLIGLSGTLNTLNGNDD1477 pKa = 4.39 GDD1479 pKa = 4.36 DD1480 pKa = 3.84 TLSGNLDD1487 pKa = 3.58 GDD1489 pKa = 4.21 SLIGGDD1495 pKa = 4.72 GSDD1498 pKa = 3.38 TLDD1501 pKa = 3.35 YY1502 pKa = 7.98 TTANAALTVDD1512 pKa = 5.08 LGTQTLFKK1520 pKa = 10.38 SASSSAKK1527 pKa = 10.04 DD1528 pKa = 3.71 YY1529 pKa = 10.89 YY1530 pKa = 11.29 SEE1532 pKa = 5.19 AEE1534 pKa = 4.3 VIVTGTYY1541 pKa = 10.33 NDD1543 pKa = 3.82 SFTLSTSTDD1552 pKa = 3.37 TAAMTLDD1559 pKa = 3.83 GGAGG1563 pKa = 3.22
Molecular weight: 159.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.528
IPC_protein 3.605
Toseland 3.363
ProMoST 3.783
Dawson 3.617
Bjellqvist 3.77
Wikipedia 3.579
Rodwell 3.427
Grimsley 3.261
Solomon 3.617
Lehninger 3.567
Nozaki 3.719
DTASelect 4.037
Thurlkill 3.414
EMBOSS 3.579
Sillero 3.732
Patrickios 0.82
IPC_peptide 3.605
IPC2_peptide 3.706
IPC2.peptide.svr19 3.688
Protein with the highest isoelectric point:
>tr|A0A2D3W529|A0A2D3W529_9PROT Uncharacterized protein OS=Sulfurospirillum cavolei OX=366522 GN=CFH80_04675 PE=4 SV=1
MM1 pKa = 6.73 TTMEE5 pKa = 4.73 KK6 pKa = 9.97 IKK8 pKa = 10.74 FEE10 pKa = 4.5 SKK12 pKa = 8.79 SHH14 pKa = 5.22 FRR16 pKa = 11.84 AFARR20 pKa = 11.84 AKK22 pKa = 10.78 LEE24 pKa = 4.07 TLNGCYY30 pKa = 9.93 RR31 pKa = 11.84 RR32 pKa = 11.84 DD33 pKa = 3.53 KK34 pKa = 10.95 LLQRR38 pKa = 11.84 QLSSLLFHH46 pKa = 6.11 MRR48 pKa = 11.84 VRR50 pKa = 11.84 SILLYY55 pKa = 10.68 NPLAIEE61 pKa = 3.89 ADD63 pKa = 3.37 IRR65 pKa = 11.84 SVIHH69 pKa = 4.29 TCRR72 pKa = 11.84 RR73 pKa = 11.84 RR74 pKa = 11.84 KK75 pKa = 10.23 CKK77 pKa = 9.93 VHH79 pKa = 6.19 VPFMEE84 pKa = 5.25 GISFKK89 pKa = 9.99 MVEE92 pKa = 3.6 WRR94 pKa = 11.84 LPLEE98 pKa = 3.9 KK99 pKa = 9.83 RR100 pKa = 11.84 VFNILEE106 pKa = 4.25 PKK108 pKa = 10.56 NSFKK112 pKa = 11.0 LKK114 pKa = 10.29 PKK116 pKa = 9.8 IDD118 pKa = 3.82 LAVVPVIGVDD128 pKa = 3.66 GAFKK132 pKa = 10.63 RR133 pKa = 11.84 VGFGKK138 pKa = 10.6 GMYY141 pKa = 10.17 DD142 pKa = 4.29 RR143 pKa = 11.84 FFQTLAKK150 pKa = 9.83 KK151 pKa = 10.08 PPIVFIQRR159 pKa = 11.84 EE160 pKa = 4.08 LCMTQSRR167 pKa = 11.84 LSEE170 pKa = 4.12 EE171 pKa = 3.9 HH172 pKa = 7.44 DD173 pKa = 3.31 IAADD177 pKa = 3.46 IYY179 pKa = 8.69 LTPYY183 pKa = 8.5 HH184 pKa = 6.41 TIIKK188 pKa = 9.93 RR189 pKa = 11.84 GEE191 pKa = 3.76 NGPRR195 pKa = 11.84 VVSRR199 pKa = 11.84 RR200 pKa = 11.84 WSRR203 pKa = 11.84 ISQRR207 pKa = 11.84 RR208 pKa = 11.84 CRR210 pKa = 11.84 IFHH213 pKa = 5.73 RR214 pKa = 11.84 QEE216 pKa = 4.06 DD217 pKa = 4.29 GSRR220 pKa = 11.84 KK221 pKa = 9.5 LL222 pKa = 3.54
Molecular weight: 26.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.633
IPC_protein 10.175
Toseland 10.774
ProMoST 10.365
Dawson 10.833
Bjellqvist 10.511
Wikipedia 11.008
Rodwell 11.155
Grimsley 10.877
Solomon 10.935
Lehninger 10.921
Nozaki 10.745
DTASelect 10.496
Thurlkill 10.745
EMBOSS 11.155
Sillero 10.774
Patrickios 10.877
IPC_peptide 10.95
IPC2_peptide 9.443
IPC2.peptide.svr19 8.682
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1874
0
1874
538432
26
1563
287.3
32.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.255 ± 0.06
1.158 ± 0.024
5.115 ± 0.046
6.873 ± 0.066
5.019 ± 0.051
6.202 ± 0.061
2.326 ± 0.03
7.38 ± 0.048
6.989 ± 0.062
10.412 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.679 ± 0.023
4.111 ± 0.037
3.259 ± 0.035
3.288 ± 0.031
4.02 ± 0.037
6.432 ± 0.045
5.284 ± 0.041
6.656 ± 0.046
0.838 ± 0.018
3.687 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here