Sulfurospirillum cavolei

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae; Sulfurospirillum

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1874 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2D3WDG9|A0A2D3WDG9_9PROT Nitrogenase molybdenum-cofactor biosynthesis protein NifE OS=Sulfurospirillum cavolei OX=366522 GN=CFH80_01145 PE=3 SV=1
MM1 pKa = 7.46SNAPEE6 pKa = 4.15VIYY9 pKa = 10.9SKK11 pKa = 10.02STISLKK17 pKa = 10.65SLPAKK22 pKa = 10.51GEE24 pKa = 3.89NLNLFLRR31 pKa = 11.84PGDD34 pKa = 4.08EE35 pKa = 4.68LSLALDD41 pKa = 3.82LSKK44 pKa = 11.41AKK46 pKa = 10.29FQVVGGDD53 pKa = 4.01IIATLPSGGQLTFVSLGMMAFEE75 pKa = 4.83NNAPVIKK82 pKa = 10.38LPNGTFLALEE92 pKa = 4.36QILDD96 pKa = 4.06TITDD100 pKa = 3.57VGQATKK106 pKa = 10.82DD107 pKa = 3.37AVLVSGNVSLEE118 pKa = 4.13TQEE121 pKa = 4.32QPIEE125 pKa = 4.15HH126 pKa = 5.86EE127 pKa = 4.51QKK129 pKa = 11.08AKK131 pKa = 9.36TQDD134 pKa = 3.39APVNDD139 pKa = 3.62YY140 pKa = 10.52NAYY143 pKa = 10.14YY144 pKa = 10.55VDD146 pKa = 3.91PEE148 pKa = 4.29LPKK151 pKa = 10.8RR152 pKa = 11.84LDD154 pKa = 3.61DD155 pKa = 3.79VASKK159 pKa = 11.11DD160 pKa = 3.59EE161 pKa = 4.27SGKK164 pKa = 10.22YY165 pKa = 9.25LQEE168 pKa = 4.18SLPEE172 pKa = 3.99FTSNEE177 pKa = 4.05TAKK180 pKa = 10.85EE181 pKa = 3.89DD182 pKa = 3.62LTNKK186 pKa = 9.89YY187 pKa = 10.31SSSEE191 pKa = 4.08EE192 pKa = 3.84KK193 pKa = 10.37TKK195 pKa = 11.48DD196 pKa = 3.21NVADD200 pKa = 3.69VSAALSFDD208 pKa = 3.28VGFYY212 pKa = 9.93QIKK215 pKa = 10.22GSEE218 pKa = 4.14STADD222 pKa = 3.14SVTSVLGGTGSALGNVSKK240 pKa = 10.81LAAAQFEE247 pKa = 4.91PEE249 pKa = 3.97TLDD252 pKa = 3.54YY253 pKa = 11.18RR254 pKa = 11.84NSAYY258 pKa = 9.75STVITADD265 pKa = 2.89NPAFVNATYY274 pKa = 8.72LTKK277 pKa = 10.53LVRR280 pKa = 11.84LNVSQPIGFTITSIAINGLTSGGFEE305 pKa = 4.13ILNSDD310 pKa = 4.59FSSAGNASGGWTLSAGTGFSSTLSDD335 pKa = 3.13GGEE338 pKa = 4.0TIEE341 pKa = 5.3FYY343 pKa = 10.54IRR345 pKa = 11.84YY346 pKa = 9.32YY347 pKa = 9.81PXNFDD352 pKa = 3.96NLMQVALTSEE362 pKa = 4.67FNMSNVPEE370 pKa = 4.26EE371 pKa = 4.13KK372 pKa = 10.18QGDD375 pKa = 4.34VIVPDD380 pKa = 3.79LTKK383 pKa = 10.06LTAYY387 pKa = 9.84KK388 pKa = 10.49DD389 pKa = 2.8IGVIVKK395 pKa = 9.98EE396 pKa = 3.96VDD398 pKa = 3.41SVSDD402 pKa = 3.33YY403 pKa = 11.0TYY405 pKa = 10.4TGKK408 pKa = 10.15HH409 pKa = 3.99STGFVLDD416 pKa = 3.61TTPNEE421 pKa = 4.27NIVYY425 pKa = 9.12TSKK428 pKa = 11.08LDD430 pKa = 3.56STVTGGLSNDD440 pKa = 3.18TMYY443 pKa = 11.48GSIGNDD449 pKa = 3.0TLEE452 pKa = 4.48GSNGNDD458 pKa = 3.39TLSGGAGVNTLIGGSGIDD476 pKa = 3.2TADD479 pKa = 3.18YY480 pKa = 11.46SFVTKK485 pKa = 10.88YY486 pKa = 11.1SDD488 pKa = 4.42DD489 pKa = 3.72FLFANDD495 pKa = 3.52GHH497 pKa = 6.94IDD499 pKa = 3.68PVTGVAYY506 pKa = 10.35ANDD509 pKa = 3.91SKK511 pKa = 11.64GVIVDD516 pKa = 4.25LQAGAASGKK525 pKa = 7.32TVYY528 pKa = 10.56DD529 pKa = 3.39ATSDD533 pKa = 3.66VLTGEE538 pKa = 4.3EE539 pKa = 4.11SRR541 pKa = 11.84TISDD545 pKa = 3.52HH546 pKa = 6.69LSGIEE551 pKa = 3.75YY552 pKa = 10.66VVGSKK557 pKa = 10.84YY558 pKa = 10.93DD559 pKa = 3.34DD560 pKa = 4.28TIRR563 pKa = 11.84GDD565 pKa = 3.46ANANKK570 pKa = 10.35LSGGEE575 pKa = 4.2GNDD578 pKa = 3.53TLEE581 pKa = 4.68GRR583 pKa = 11.84DD584 pKa = 3.54GKK586 pKa = 10.71DD587 pKa = 2.77WLEE590 pKa = 4.27GGAGNDD596 pKa = 3.41WLIGSTDD603 pKa = 3.78DD604 pKa = 4.19YY605 pKa = 11.13MIDD608 pKa = 4.1GGDD611 pKa = 3.63NTDD614 pKa = 3.58TADD617 pKa = 4.3FSNNSNGLIITLNDD631 pKa = 3.45SANGSLGNIGGTSATTIIKK650 pKa = 10.21NVEE653 pKa = 4.06NVAGTQYY660 pKa = 11.09KK661 pKa = 10.69DD662 pKa = 3.69SISGDD667 pKa = 3.45TADD670 pKa = 3.99NLLIGGYY677 pKa = 8.44DD678 pKa = 3.41HH679 pKa = 7.47SATAVTANDD688 pKa = 3.51DD689 pKa = 4.28TITGGSGADD698 pKa = 3.56TIVGDD703 pKa = 3.79MMIDD707 pKa = 3.54TVIATPLYY715 pKa = 10.59AGNDD719 pKa = 3.67VLGGGSDD726 pKa = 3.51NDD728 pKa = 4.3TIYY731 pKa = 11.34GDD733 pKa = 3.78SAPILSNAEE742 pKa = 3.82VEE744 pKa = 4.64VAADD748 pKa = 3.34QSVEE752 pKa = 4.22YY753 pKa = 10.36IRR755 pKa = 11.84NTDD758 pKa = 3.85DD759 pKa = 5.19DD760 pKa = 4.25STLATIYY767 pKa = 10.76GGNDD771 pKa = 3.16TLKK774 pKa = 11.09GGGGDD779 pKa = 3.93DD780 pKa = 3.89YY781 pKa = 11.87LNGGSGWDD789 pKa = 3.5TVDD792 pKa = 3.7YY793 pKa = 10.16SASAAAVIVNLSSNYY808 pKa = 10.77AMGEE812 pKa = 4.29GYY814 pKa = 10.29DD815 pKa = 3.27QLFNIEE821 pKa = 4.82NIVGSNSALGDD832 pKa = 3.76TLIGNALANTIVGSTSGADD851 pKa = 3.46TLSGMGGLDD860 pKa = 3.38TLNGLGGKK868 pKa = 9.16DD869 pKa = 3.01WADD872 pKa = 3.31YY873 pKa = 10.79SYY875 pKa = 11.96VSGTTGVNVDD885 pKa = 3.95LTAGTAYY892 pKa = 10.62VSATDD897 pKa = 3.85GDD899 pKa = 4.01TLIAIEE905 pKa = 4.11NVIGSSKK912 pKa = 11.18ADD914 pKa = 3.66TLSGGSGNSVNTLMGGLGNDD934 pKa = 2.7IFYY937 pKa = 10.54GYY939 pKa = 11.14GDD941 pKa = 4.31GDD943 pKa = 4.29LLDD946 pKa = 4.96GGSGSDD952 pKa = 3.19TADD955 pKa = 3.03YY956 pKa = 9.17MYY958 pKa = 10.8AQGRR962 pKa = 11.84IVVTLDD968 pKa = 3.22GSNSDD973 pKa = 3.01TLMNIEE979 pKa = 4.19NVYY982 pKa = 10.26GASDD986 pKa = 3.95KK987 pKa = 10.63DD988 pKa = 3.81TLIGNSDD995 pKa = 3.54ANILDD1000 pKa = 3.56GRR1002 pKa = 11.84ADD1004 pKa = 3.79TDD1006 pKa = 3.78TLSGMGGADD1015 pKa = 3.53TLIGGAGDD1023 pKa = 3.53DD1024 pKa = 4.13TLIGGAGNDD1033 pKa = 4.2TLWGGTDD1040 pKa = 3.24ATHH1043 pKa = 8.06DD1044 pKa = 3.98SGIDD1048 pKa = 3.18TADD1051 pKa = 3.4YY1052 pKa = 10.62SGSSSIYY1059 pKa = 10.32ADD1061 pKa = 3.66LANGTVNDD1069 pKa = 4.43GLGGIDD1075 pKa = 3.34TLAGIEE1081 pKa = 4.34VILGSGSNDD1090 pKa = 3.15VMIGSGNADD1099 pKa = 3.46TLIGNGGNDD1108 pKa = 3.16TFFASGGGDD1117 pKa = 3.04SYY1119 pKa = 11.78YY1120 pKa = 11.16GGVFGSSSDD1129 pKa = 3.65GNVKK1133 pKa = 10.46DD1134 pKa = 3.74RR1135 pKa = 11.84LSYY1138 pKa = 11.09ADD1140 pKa = 4.04VYY1142 pKa = 11.34HH1143 pKa = 7.34DD1144 pKa = 4.61VIDD1147 pKa = 4.27SKK1149 pKa = 10.07TVDD1152 pKa = 4.19HH1153 pKa = 6.67IVVDD1157 pKa = 5.01LSHH1160 pKa = 6.92VGTNATILDD1169 pKa = 3.76ASNAVISTDD1178 pKa = 2.83SLYY1181 pKa = 10.84AIEE1184 pKa = 5.43EE1185 pKa = 4.16IVATDD1190 pKa = 3.64GNDD1193 pKa = 3.52TLRR1196 pKa = 11.84GGAGSQQTFYY1206 pKa = 11.34GGAGDD1211 pKa = 4.54DD1212 pKa = 3.87TLSGNTDD1219 pKa = 2.74GDD1221 pKa = 4.11YY1222 pKa = 11.5LXTNLADD1229 pKa = 3.58YY1230 pKa = 7.63TAQSSNLVVNLASTSNNVYY1249 pKa = 10.65LSGSSPTSSNSDD1261 pKa = 2.93TLANINNIATGSGADD1276 pKa = 3.63TIIGNGNDD1284 pKa = 3.53NIVRR1288 pKa = 11.84AGVGADD1294 pKa = 3.62TLSGGAGDD1302 pKa = 3.63DD1303 pKa = 3.69TLYY1306 pKa = 11.52GEE1308 pKa = 5.21AGNDD1312 pKa = 3.54TLSGGEE1318 pKa = 4.39GNDD1321 pKa = 3.55TLIGGMSTSVDD1332 pKa = 3.57SGIDD1336 pKa = 3.28TADD1339 pKa = 3.47YY1340 pKa = 11.04SATSYY1345 pKa = 11.43YY1346 pKa = 10.69GVTVDD1351 pKa = 4.72LQNGNATGATIGTDD1365 pKa = 2.84KK1366 pKa = 11.15LYY1368 pKa = 11.02GIEE1371 pKa = 4.11NVLGSAQSDD1380 pKa = 3.92TLIGASGTLNTLSGLGGNDD1399 pKa = 2.99TLYY1402 pKa = 11.53GNLEE1406 pKa = 3.76GDD1408 pKa = 4.04YY1409 pKa = 10.95LDD1411 pKa = 5.23GGSGTNTADD1420 pKa = 3.6YY1421 pKa = 10.85SSEE1424 pKa = 4.02TASIVANLTTGRR1436 pKa = 11.84AYY1438 pKa = 8.58FTSSSSYY1445 pKa = 10.66DD1446 pKa = 3.37ALVSIQNVLSGSGNDD1461 pKa = 3.65TLIGLSGTLNTLNGNDD1477 pKa = 4.39GDD1479 pKa = 4.36DD1480 pKa = 3.84TLSGNLDD1487 pKa = 3.58GDD1489 pKa = 4.21SLIGGDD1495 pKa = 4.72GSDD1498 pKa = 3.38TLDD1501 pKa = 3.35YY1502 pKa = 7.98TTANAALTVDD1512 pKa = 5.08LGTQTLFKK1520 pKa = 10.38SASSSAKK1527 pKa = 10.04DD1528 pKa = 3.71YY1529 pKa = 10.89YY1530 pKa = 11.29SEE1532 pKa = 5.19AEE1534 pKa = 4.3VIVTGTYY1541 pKa = 10.33NDD1543 pKa = 3.82SFTLSTSTDD1552 pKa = 3.37TAAMTLDD1559 pKa = 3.83GGAGG1563 pKa = 3.22

Molecular weight:
159.4 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2D3W529|A0A2D3W529_9PROT Uncharacterized protein OS=Sulfurospirillum cavolei OX=366522 GN=CFH80_04675 PE=4 SV=1
MM1 pKa = 6.73TTMEE5 pKa = 4.73KK6 pKa = 9.97IKK8 pKa = 10.74FEE10 pKa = 4.5SKK12 pKa = 8.79SHH14 pKa = 5.22FRR16 pKa = 11.84AFARR20 pKa = 11.84AKK22 pKa = 10.78LEE24 pKa = 4.07TLNGCYY30 pKa = 9.93RR31 pKa = 11.84RR32 pKa = 11.84DD33 pKa = 3.53KK34 pKa = 10.95LLQRR38 pKa = 11.84QLSSLLFHH46 pKa = 6.11MRR48 pKa = 11.84VRR50 pKa = 11.84SILLYY55 pKa = 10.68NPLAIEE61 pKa = 3.89ADD63 pKa = 3.37IRR65 pKa = 11.84SVIHH69 pKa = 4.29TCRR72 pKa = 11.84RR73 pKa = 11.84RR74 pKa = 11.84KK75 pKa = 10.23CKK77 pKa = 9.93VHH79 pKa = 6.19VPFMEE84 pKa = 5.25GISFKK89 pKa = 9.99MVEE92 pKa = 3.6WRR94 pKa = 11.84LPLEE98 pKa = 3.9KK99 pKa = 9.83RR100 pKa = 11.84VFNILEE106 pKa = 4.25PKK108 pKa = 10.56NSFKK112 pKa = 11.0LKK114 pKa = 10.29PKK116 pKa = 9.8IDD118 pKa = 3.82LAVVPVIGVDD128 pKa = 3.66GAFKK132 pKa = 10.63RR133 pKa = 11.84VGFGKK138 pKa = 10.6GMYY141 pKa = 10.17DD142 pKa = 4.29RR143 pKa = 11.84FFQTLAKK150 pKa = 9.83KK151 pKa = 10.08PPIVFIQRR159 pKa = 11.84EE160 pKa = 4.08LCMTQSRR167 pKa = 11.84LSEE170 pKa = 4.12EE171 pKa = 3.9HH172 pKa = 7.44DD173 pKa = 3.31IAADD177 pKa = 3.46IYY179 pKa = 8.69LTPYY183 pKa = 8.5HH184 pKa = 6.41TIIKK188 pKa = 9.93RR189 pKa = 11.84GEE191 pKa = 3.76NGPRR195 pKa = 11.84VVSRR199 pKa = 11.84RR200 pKa = 11.84WSRR203 pKa = 11.84ISQRR207 pKa = 11.84RR208 pKa = 11.84CRR210 pKa = 11.84IFHH213 pKa = 5.73RR214 pKa = 11.84QEE216 pKa = 4.06DD217 pKa = 4.29GSRR220 pKa = 11.84KK221 pKa = 9.5LL222 pKa = 3.54

Molecular weight:
26.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1874

0

1874

538432

26

1563

287.3

32.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.255 ± 0.06

1.158 ± 0.024

5.115 ± 0.046

6.873 ± 0.066

5.019 ± 0.051

6.202 ± 0.061

2.326 ± 0.03

7.38 ± 0.048

6.989 ± 0.062

10.412 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.679 ± 0.023

4.111 ± 0.037

3.259 ± 0.035

3.288 ± 0.031

4.02 ± 0.037

6.432 ± 0.045

5.284 ± 0.041

6.656 ± 0.046

0.838 ± 0.018

3.687 ± 0.036

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski