Poecilia reticulata (Guppy) (Acanthophacelus reticulatus)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleosteomorpha; Neoteleostei;

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 33944 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P9MV63|A0A3P9MV63_POERE Nectin-4-like OS=Poecilia reticulata OX=8081 PE=4 SV=1
FF1 pKa = 7.41LKK3 pKa = 9.55NTVMEE8 pKa = 4.7CEE10 pKa = 3.94ACGMGGIQPVSPCEE24 pKa = 4.04PNPCHH29 pKa = 6.51PGVRR33 pKa = 11.84CSEE36 pKa = 4.3SPEE39 pKa = 4.23GPQCGACPDD48 pKa = 3.54GMEE51 pKa = 4.81GNGTRR56 pKa = 11.84CTDD59 pKa = 3.04VDD61 pKa = 3.71EE62 pKa = 5.13CKK64 pKa = 10.89VMPCHH69 pKa = 6.36MGVRR73 pKa = 11.84CINTSPGFRR82 pKa = 11.84CGSCPAGYY90 pKa = 8.41TGPQVQGVGLAYY102 pKa = 9.21ATANKK107 pKa = 9.14QVCRR111 pKa = 11.84DD112 pKa = 3.05IDD114 pKa = 3.69EE115 pKa = 5.41CEE117 pKa = 4.05GSSNGGCVEE126 pKa = 4.67NSVCLNTPGSFRR138 pKa = 11.84CGPCKK143 pKa = 9.85PGYY146 pKa = 10.65VGDD149 pKa = 3.78QRR151 pKa = 11.84LGCRR155 pKa = 11.84PEE157 pKa = 4.03RR158 pKa = 11.84TCGNGQPNPCHH169 pKa = 6.72ASADD173 pKa = 4.22CIVHH177 pKa = 6.53RR178 pKa = 11.84EE179 pKa = 4.11GTIEE183 pKa = 4.18CQCGVGWAGNGYY195 pKa = 9.9LCATDD200 pKa = 3.48TDD202 pKa = 3.7IDD204 pKa = 3.98GFPDD208 pKa = 3.5EE209 pKa = 5.98RR210 pKa = 11.84LDD212 pKa = 5.83CPDD215 pKa = 3.73LTCNKK220 pKa = 9.57DD221 pKa = 3.22NCLTVPNSGQEE232 pKa = 3.99DD233 pKa = 4.13ADD235 pKa = 3.65NDD237 pKa = 5.1GIGDD241 pKa = 4.07ACDD244 pKa = 4.46DD245 pKa = 4.56DD246 pKa = 6.96ADD248 pKa = 4.58GDD250 pKa = 4.67GIPNTQDD257 pKa = 2.74NCKK260 pKa = 9.99LVPNVDD266 pKa = 3.27QTNIDD271 pKa = 3.44EE272 pKa = 5.46DD273 pKa = 4.52DD274 pKa = 4.85FGDD277 pKa = 4.0ACDD280 pKa = 3.29NCRR283 pKa = 11.84AVKK286 pKa = 10.88NNDD289 pKa = 3.36QKK291 pKa = 11.3DD292 pKa = 3.45TDD294 pKa = 4.1LDD296 pKa = 3.97KK297 pKa = 11.72LGDD300 pKa = 3.86EE301 pKa = 5.26CDD303 pKa = 3.48EE304 pKa = 5.27DD305 pKa = 3.85MDD307 pKa = 5.78GDD309 pKa = 4.5GIINVMDD316 pKa = 3.41NCKK319 pKa = 9.88RR320 pKa = 11.84VPNVDD325 pKa = 3.03QKK327 pKa = 11.72DD328 pKa = 3.56RR329 pKa = 11.84DD330 pKa = 3.58GDD332 pKa = 4.12KK333 pKa = 11.46VGDD336 pKa = 4.05ACDD339 pKa = 3.38SCPYY343 pKa = 10.12VPNPDD348 pKa = 4.61QMDD351 pKa = 4.03ADD353 pKa = 4.07NDD355 pKa = 4.82LIGDD359 pKa = 3.97PCDD362 pKa = 3.62TNKK365 pKa = 10.93DD366 pKa = 3.49SDD368 pKa = 4.16GDD370 pKa = 3.81GHH372 pKa = 6.91QDD374 pKa = 4.2SRR376 pKa = 11.84DD377 pKa = 3.47NCPAVINSSQLDD389 pKa = 3.5TDD391 pKa = 3.78KK392 pKa = 11.37DD393 pKa = 4.07GKK395 pKa = 11.12GDD397 pKa = 3.71EE398 pKa = 5.18CDD400 pKa = 5.95DD401 pKa = 5.62DD402 pKa = 7.29DD403 pKa = 7.55DD404 pKa = 7.26DD405 pKa = 7.31DD406 pKa = 6.25GIPDD410 pKa = 5.2LLPPGPDD417 pKa = 2.92NCRR420 pKa = 11.84LVPNPLQEE428 pKa = 5.34DD429 pKa = 4.06LDD431 pKa = 5.15GDD433 pKa = 4.37GVGDD437 pKa = 3.69VCEE440 pKa = 4.78KK441 pKa = 11.14DD442 pKa = 3.38FDD444 pKa = 4.17NDD446 pKa = 4.31TIIDD450 pKa = 5.0PIDD453 pKa = 3.84ACPEE457 pKa = 3.91NAEE460 pKa = 4.1VTLTDD465 pKa = 3.59FRR467 pKa = 11.84EE468 pKa = 4.27YY469 pKa = 9.18QTVVLDD475 pKa = 4.15PEE477 pKa = 5.32GDD479 pKa = 3.65AQIDD483 pKa = 4.13PNWVVLNQGRR493 pKa = 11.84EE494 pKa = 3.9IVQTMNSDD502 pKa = 3.19PGLAVGYY509 pKa = 7.12TAFSGVDD516 pKa = 3.72FEE518 pKa = 5.24GTFHH522 pKa = 6.81VNTVTDD528 pKa = 3.57DD529 pKa = 4.08DD530 pKa = 4.35YY531 pKa = 12.0AGFIFGYY538 pKa = 9.58QDD540 pKa = 2.83SSSFYY545 pKa = 9.91VVMWKK550 pKa = 9.52QVEE553 pKa = 4.18QIYY556 pKa = 8.33WQANPFRR563 pKa = 11.84AVAQQGIQLKK573 pKa = 9.81AVKK576 pKa = 10.16SSTGPGEE583 pKa = 4.16NLRR586 pKa = 11.84NALWHH591 pKa = 6.37TGDD594 pKa = 3.91TGDD597 pKa = 4.85QVKK600 pKa = 10.34LLWKK604 pKa = 10.29DD605 pKa = 3.3PRR607 pKa = 11.84NVGWKK612 pKa = 10.34DD613 pKa = 3.02KK614 pKa = 10.83TSYY617 pKa = 10.52RR618 pKa = 11.84WFLQHH623 pKa = 6.87RR624 pKa = 11.84PADD627 pKa = 3.73GYY629 pKa = 10.45IRR631 pKa = 11.84VRR633 pKa = 11.84FYY635 pKa = 11.04EE636 pKa = 4.86GPQMVADD643 pKa = 3.78TGVIIDD649 pKa = 3.35ATMRR653 pKa = 11.84GGRR656 pKa = 11.84LGVFCFSQEE665 pKa = 3.95NIIWANLRR673 pKa = 11.84YY674 pKa = 9.71RR675 pKa = 11.84CNDD678 pKa = 3.51TLPGDD683 pKa = 3.68FDD685 pKa = 3.85TYY687 pKa = 10.14RR688 pKa = 11.84TQQVQLVAA696 pKa = 4.41

Molecular weight: 75.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P9PAF3|A0A3P9PAF3_POERE Uncharacterized protein OS=Poecilia reticulata OX=8081 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 10.08RR15 pKa = 11.84KK16 pKa = 8.21RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 8.46MRR21 pKa = 11.84QRR23 pKa = 11.84SKK25 pKa = 11.41

Molecular weight: 3.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22853

11091

33944

19349097

9

6188

570.0

63.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.505 ± 0.011

2.274 ± 0.011

5.185 ± 0.008

6.774 ± 0.016

3.796 ± 0.009

6.309 ± 0.016

2.615 ± 0.007

4.395 ± 0.009

5.685 ± 0.014

9.606 ± 0.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.005

3.888 ± 0.008

5.669 ± 0.016

4.699 ± 0.011

5.661 ± 0.011

8.774 ± 0.017

5.523 ± 0.008

6.319 ± 0.011

1.161 ± 0.004

2.721 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski