Amino acid dipepetide frequency for Poecilia reticulata (Guppy) (Acanthophacelus reticulatus)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.498AlaAla: 6.498 ± 0.037	1.277AlaCys: 1.277 ± 0.009	3.206AlaAsp: 3.206 ± 0.013	4.629AlaGlu: 4.629 ± 0.021	2.436AlaPhe: 2.436 ± 0.011	4.359AlaGly: 4.359 ± 0.023	1.448AlaHis: 1.448 ± 0.009	2.63AlaIle: 2.63 ± 0.015	3.297AlaLys: 3.297 ± 0.017	6.391AlaLeu: 6.391 ± 0.022	1.54AlaMet: 1.54 ± 0.009	2.147AlaAsn: 2.147 ± 0.011	3.474AlaPro: 3.474 ± 0.019	2.841AlaGln: 2.841 ± 0.014	3.03AlaArg: 3.03 ± 0.015	5.418AlaSer: 5.418 ± 0.023	3.373AlaThr: 3.373 ± 0.016	4.903AlaVal: 4.903 ± 0.02	0.646AlaTrp: 0.646 ± 0.006	1.481AlaTyr: 1.481 ± 0.01	0.03AlaXaa: 0.03 ± 0.001
Cys	1.153CysAla: 1.153 ± 0.009	0.701CysCys: 0.701 ± 0.009	1.142CysAsp: 1.142 ± 0.012	1.281CysGlu: 1.281 ± 0.012	0.982CysPhe: 0.982 ± 0.008	1.656CysGly: 1.656 ± 0.015	0.627CysHis: 0.627 ± 0.007	0.99CysIle: 0.99 ± 0.008	1.151CysLys: 1.151 ± 0.009	2.225CysLeu: 2.225 ± 0.013	0.466CysMet: 0.466 ± 0.005	0.842CysAsn: 0.842 ± 0.008	1.287CysPro: 1.287 ± 0.012	1.019CysGln: 1.019 ± 0.01	1.318CysArg: 1.318 ± 0.011	2.274CysSer: 2.274 ± 0.013	1.125CysThr: 1.125 ± 0.008	1.568CysVal: 1.568 ± 0.014	0.305CysTrp: 0.305 ± 0.004	0.617CysTyr: 0.617 ± 0.006	0.011CysXaa: 0.011 ± 0.001
Asp	2.962AspAla: 2.962 ± 0.012	1.148AspCys: 1.148 ± 0.01	3.138AspAsp: 3.138 ± 0.023	3.783AspGlu: 3.783 ± 0.017	2.201AspPhe: 2.201 ± 0.013	3.8AspGly: 3.8 ± 0.02	1.163AspHis: 1.163 ± 0.009	2.619AspIle: 2.619 ± 0.012	2.701AspLys: 2.701 ± 0.014	5.021AspLeu: 5.021 ± 0.017	1.241AspMet: 1.241 ± 0.009	1.92AspAsn: 1.92 ± 0.012	2.872AspPro: 2.872 ± 0.015	1.994AspGln: 1.994 ± 0.011	2.8AspArg: 2.8 ± 0.015	4.452AspSer: 4.452 ± 0.019	2.453AspThr: 2.453 ± 0.011	3.341AspVal: 3.341 ± 0.014	0.685AspTrp: 0.685 ± 0.008	1.532AspTyr: 1.532 ± 0.009	0.028AspXaa: 0.028 ± 0.001
Glu	4.648GluAla: 4.648 ± 0.022	1.181GluCys: 1.181 ± 0.012	4.379GluAsp: 4.379 ± 0.017	7.537GluGlu: 7.537 ± 0.052	1.987GluPhe: 1.987 ± 0.011	3.925GluGly: 3.925 ± 0.019	1.412GluHis: 1.412 ± 0.009	2.837GluIle: 2.837 ± 0.015	4.832GluLys: 4.832 ± 0.029	6.069GluLeu: 6.069 ± 0.027	1.714GluMet: 1.714 ± 0.011	2.829GluAsn: 2.829 ± 0.015	2.814GluPro: 2.814 ± 0.015	3.021GluGln: 3.021 ± 0.018	4.208GluArg: 4.208 ± 0.024	4.373GluSer: 4.373 ± 0.018	3.501GluThr: 3.501 ± 0.018	4.23GluVal: 4.23 ± 0.017	0.67GluTrp: 0.67 ± 0.006	1.545GluTyr: 1.545 ± 0.01	0.029GluXaa: 0.029 ± 0.001
Phe	1.959PheAla: 1.959 ± 0.012	1.065PheCys: 1.065 ± 0.008	1.802PheAsp: 1.802 ± 0.011	1.875PheGlu: 1.875 ± 0.01	1.773PhePhe: 1.773 ± 0.013	2.241PheGly: 2.241 ± 0.013	1.046PheHis: 1.046 ± 0.008	2.02PheIle: 2.02 ± 0.013	1.861PheLys: 1.861 ± 0.013	4.099PheLeu: 4.099 ± 0.022	0.852PheMet: 0.852 ± 0.007	1.545PheAsn: 1.545 ± 0.01	1.956PhePro: 1.956 ± 0.012	1.695PheGln: 1.695 ± 0.01	1.957PheArg: 1.957 ± 0.009	3.725PheSer: 3.725 ± 0.018	2.335PheThr: 2.335 ± 0.015	2.334PheVal: 2.334 ± 0.013	0.517PheTrp: 0.517 ± 0.006	1.281PheTyr: 1.281 ± 0.009	0.019PheXaa: 0.019 ± 0.001
Gly	3.972GlyAla: 3.972 ± 0.023	1.257GlyCys: 1.257 ± 0.011	3.245GlyAsp: 3.245 ± 0.015	4.131GlyGlu: 4.131 ± 0.025	2.518GlyPhe: 2.518 ± 0.015	5.227GlyGly: 5.227 ± 0.031	1.616GlyHis: 1.616 ± 0.011	2.592GlyIle: 2.592 ± 0.013	3.659GlyLys: 3.659 ± 0.02	5.481GlyLeu: 5.481 ± 0.022	1.432GlyMet: 1.432 ± 0.011	2.469GlyAsn: 2.469 ± 0.013	3.327GlyPro: 3.327 ± 0.036	2.713GlyGln: 2.713 ± 0.015	3.67GlyArg: 3.67 ± 0.016	5.906GlySer: 5.906 ± 0.024	3.317GlyThr: 3.317 ± 0.017	3.963GlyVal: 3.963 ± 0.019	0.778GlyTrp: 0.778 ± 0.007	1.788GlyTyr: 1.788 ± 0.013	0.028GlyXaa: 0.028 ± 0.001
His	1.305HisAla: 1.305 ± 0.009	0.725HisCys: 0.725 ± 0.007	0.968HisAsp: 0.968 ± 0.007	1.18HisGlu: 1.18 ± 0.009	1.101HisPhe: 1.101 ± 0.008	1.538HisGly: 1.538 ± 0.013	1.064HisHis: 1.064 ± 0.013	1.276HisIle: 1.276 ± 0.009	1.299HisLys: 1.299 ± 0.009	2.738HisLeu: 2.738 ± 0.013	0.725HisMet: 0.725 ± 0.008	1.014HisAsn: 1.014 ± 0.007	1.6HisPro: 1.6 ± 0.012	1.314HisGln: 1.314 ± 0.012	1.633HisArg: 1.633 ± 0.011	2.432HisSer: 2.432 ± 0.015	1.618HisThr: 1.618 ± 0.013	1.455HisVal: 1.455 ± 0.01	0.333HisTrp: 0.333 ± 0.004	0.822HisTyr: 0.822 ± 0.007	0.012HisXaa: 0.012 ± 0.001
Ile	2.423IleAla: 2.423 ± 0.013	1.117IleCys: 1.117 ± 0.01	2.055IleAsp: 2.055 ± 0.013	2.306IleGlu: 2.306 ± 0.013	1.916IlePhe: 1.916 ± 0.013	2.302IleGly: 2.302 ± 0.012	1.317IleHis: 1.317 ± 0.01	2.423IleIle: 2.423 ± 0.016	2.574IleLys: 2.574 ± 0.014	4.338IleLeu: 4.338 ± 0.018	1.064IleMet: 1.064 ± 0.008	1.993IleAsn: 1.993 ± 0.013	2.433IlePro: 2.433 ± 0.012	2.197IleGln: 2.197 ± 0.011	2.49IleArg: 2.49 ± 0.012	3.805IleSer: 3.805 ± 0.017	2.705IleThr: 2.705 ± 0.016	2.563IleVal: 2.563 ± 0.014	0.501IleTrp: 0.501 ± 0.006	1.405IleTyr: 1.405 ± 0.011	0.02IleXaa: 0.02 ± 0.001
Lys	3.667LysAla: 3.667 ± 0.017	1.048LysCys: 1.048 ± 0.009	3.243LysAsp: 3.243 ± 0.015	4.677LysGlu: 4.677 ± 0.026	1.631LysPhe: 1.631 ± 0.011	3.22LysGly: 3.22 ± 0.023	1.439LysHis: 1.439 ± 0.009	2.552LysIle: 2.552 ± 0.014	4.481LysLys: 4.481 ± 0.028	5.038LysLeu: 5.038 ± 0.022	1.483LysMet: 1.483 ± 0.01	2.372LysAsn: 2.372 ± 0.014	3.018LysPro: 3.018 ± 0.018	2.595LysGln: 2.595 ± 0.016	3.426LysArg: 3.426 ± 0.017	3.973LysSer: 3.973 ± 0.02	3.317LysThr: 3.317 ± 0.014	3.528LysVal: 3.528 ± 0.013	0.592LysTrp: 0.592 ± 0.006	1.514LysTyr: 1.514 ± 0.009	0.033LysXaa: 0.033 ± 0.002
Leu	5.93LeuAla: 5.93 ± 0.022	2.279LeuCys: 2.279 ± 0.014	4.949LeuAsp: 4.949 ± 0.02	6.338LeuGlu: 6.338 ± 0.031	3.591LeuPhe: 3.591 ± 0.019	5.136LeuGly: 5.136 ± 0.021	2.772LeuHis: 2.772 ± 0.014	3.889LeuIle: 3.889 ± 0.016	5.681LeuLys: 5.681 ± 0.025	10.238LeuLeu: 10.238 ± 0.039	2.131LeuMet: 2.131 ± 0.011	3.691LeuAsn: 3.691 ± 0.016	5.344LeuPro: 5.344 ± 0.018	5.524LeuGln: 5.524 ± 0.024	5.631LeuArg: 5.631 ± 0.022	8.407LeuSer: 8.407 ± 0.026	5.349LeuThr: 5.349 ± 0.019	5.485LeuVal: 5.485 ± 0.022	1.064LeuTrp: 1.064 ± 0.008	2.592LeuTyr: 2.592 ± 0.015	0.042LeuXaa: 0.042 ± 0.002
Met	1.853MetAla: 1.853 ± 0.01	0.485MetCys: 0.485 ± 0.006	1.39MetAsp: 1.39 ± 0.009	1.962MetGlu: 1.962 ± 0.012	0.895MetPhe: 0.895 ± 0.008	1.367MetGly: 1.367 ± 0.01	0.482MetHis: 0.482 ± 0.005	0.916MetIle: 0.916 ± 0.008	1.51MetLys: 1.51 ± 0.009	2.082MetLeu: 2.082 ± 0.011	0.706MetMet: 0.706 ± 0.008	0.93MetAsn: 0.93 ± 0.007	1.05MetPro: 1.05 ± 0.008	0.98MetGln: 0.98 ± 0.008	1.238MetArg: 1.238 ± 0.009	1.882MetSer: 1.882 ± 0.011	1.279MetThr: 1.279 ± 0.009	1.498MetVal: 1.498 ± 0.009	0.263MetTrp: 0.263 ± 0.003	0.62MetTyr: 0.62 ± 0.006	0.009MetXaa: 0.009 ± 0.001
Asn	2.16AsnAla: 2.16 ± 0.012	0.873AsnCys: 0.873 ± 0.008	1.703AsnAsp: 1.703 ± 0.01	2.104AsnGlu: 2.104 ± 0.012	1.512AsnPhe: 1.512 ± 0.01	2.752AsnGly: 2.752 ± 0.015	1.028AsnHis: 1.028 ± 0.009	2.182AsnIle: 2.182 ± 0.011	2.278AsnLys: 2.278 ± 0.011	3.834AsnLeu: 3.834 ± 0.018	1.074AsnMet: 1.074 ± 0.008	1.839AsnAsn: 1.839 ± 0.011	2.287AsnPro: 2.287 ± 0.012	1.803AsnGln: 1.803 ± 0.01	2.035AsnArg: 2.035 ± 0.012	3.261AsnSer: 3.261 ± 0.015	2.181AsnThr: 2.181 ± 0.013	2.343AsnVal: 2.343 ± 0.011	0.456AsnTrp: 0.456 ± 0.005	1.156AsnTyr: 1.156 ± 0.009	0.023AsnXaa: 0.023 ± 0.001
Pro	4.164ProAla: 4.164 ± 0.022	1.069ProCys: 1.069 ± 0.012	2.943ProAsp: 2.943 ± 0.013	3.709ProGlu: 3.709 ± 0.016	1.949ProPhe: 1.949 ± 0.011	4.219ProGly: 4.219 ± 0.047	1.476ProHis: 1.476 ± 0.012	1.847ProIle: 1.847 ± 0.011	2.533ProLys: 2.533 ± 0.016	4.875ProLeu: 4.875 ± 0.021	0.997ProMet: 0.997 ± 0.008	1.976ProAsn: 1.976 ± 0.011	5.373ProPro: 5.373 ± 0.04	2.633ProGln: 2.633 ± 0.015	2.715ProArg: 2.715 ± 0.014	5.495ProSer: 5.495 ± 0.029	3.043ProThr: 3.043 ± 0.016	3.729ProVal: 3.729 ± 0.017	0.536ProTrp: 0.536 ± 0.006	1.382ProTyr: 1.382 ± 0.01	0.028ProXaa: 0.028 ± 0.001
Gln	3.108GlnAla: 3.108 ± 0.015	0.936GlnCys: 0.936 ± 0.01	2.293GlnAsp: 2.293 ± 0.012	3.461GlnGlu: 3.461 ± 0.019	1.384GlnPhe: 1.384 ± 0.009	2.62GlnGly: 2.62 ± 0.017	1.358GlnHis: 1.358 ± 0.011	1.966GlnIle: 1.966 ± 0.011	2.715GlnLys: 2.715 ± 0.016	4.48GlnLeu: 4.48 ± 0.024	1.149GlnMet: 1.149 ± 0.008	1.892GlnAsn: 1.892 ± 0.012	2.545GlnPro: 2.545 ± 0.017	3.338GlnGln: 3.338 ± 0.032	3.079GlnArg: 3.079 ± 0.016	3.534GlnSer: 3.534 ± 0.016	2.613GlnThr: 2.613 ± 0.013	2.785GlnVal: 2.785 ± 0.013	0.537GlnTrp: 0.537 ± 0.006	1.171GlnTyr: 1.171 ± 0.011	0.021GlnXaa: 0.021 ± 0.001
Arg	3.35ArgAla: 3.35 ± 0.014	1.289ArgCys: 1.289 ± 0.013	2.936ArgAsp: 2.936 ± 0.013	3.822ArgGlu: 3.822 ± 0.021	2.091ArgPhe: 2.091 ± 0.012	3.438ArgGly: 3.438 ± 0.022	1.586ArgHis: 1.586 ± 0.01	2.434ArgIle: 2.434 ± 0.013	3.616ArgLys: 3.616 ± 0.017	5.347ArgLeu: 5.347 ± 0.021	1.3ArgMet: 1.3 ± 0.01	2.176ArgAsn: 2.176 ± 0.012	2.959ArgPro: 2.959 ± 0.015	2.611ArgGln: 2.611 ± 0.013	4.426ArgArg: 4.426 ± 0.026	4.723ArgSer: 4.723 ± 0.023	2.982ArgThr: 2.982 ± 0.014	3.248ArgVal: 3.248 ± 0.013	0.693ArgTrp: 0.693 ± 0.006	1.559ArgTyr: 1.559 ± 0.01	0.023ArgXaa: 0.023 ± 0.001
Ser	5.747SerAla: 5.747 ± 0.022	2.143SerCys: 2.143 ± 0.015	4.423SerAsp: 4.423 ± 0.021	4.982SerGlu: 4.982 ± 0.019	3.324SerPhe: 3.324 ± 0.013	5.662SerGly: 5.662 ± 0.022	2.244SerHis: 2.244 ± 0.012	3.344SerIle: 3.344 ± 0.017	4.116SerLys: 4.116 ± 0.021	8.429SerLeu: 8.429 ± 0.027	1.78SerMet: 1.78 ± 0.01	3.111SerAsn: 3.111 ± 0.013	5.909SerPro: 5.909 ± 0.031	3.89SerGln: 3.89 ± 0.018	4.692SerArg: 4.692 ± 0.02	10.545SerSer: 10.545 ± 0.048	4.675SerThr: 4.675 ± 0.021	5.484SerVal: 5.484 ± 0.018	1.011SerTrp: 1.011 ± 0.007	2.187SerTyr: 2.187 ± 0.013	0.047SerXaa: 0.047 ± 0.002
Thr	3.926ThrAla: 3.926 ± 0.015	1.413ThrCys: 1.413 ± 0.015	2.862ThrAsp: 2.862 ± 0.014	3.67ThrGlu: 3.67 ± 0.017	2.161ThrPhe: 2.161 ± 0.013	3.766ThrGly: 3.766 ± 0.02	1.42ThrHis: 1.42 ± 0.011	2.373ThrIle: 2.373 ± 0.013	2.714ThrLys: 2.714 ± 0.016	5.268ThrLeu: 5.268 ± 0.019	1.196ThrMet: 1.196 ± 0.009	1.975ThrAsn: 1.975 ± 0.011	3.488ThrPro: 3.488 ± 0.02	2.334ThrGln: 2.334 ± 0.014	2.525ThrArg: 2.525 ± 0.011	4.779ThrSer: 4.779 ± 0.022	3.178ThrThr: 3.178 ± 0.022	4.051ThrVal: 4.051 ± 0.021	0.68ThrTrp: 0.68 ± 0.006	1.427ThrTyr: 1.427 ± 0.011	0.026ThrXaa: 0.026 ± 0.001
Val	4.132ValAla: 4.132 ± 0.017	1.755ValCys: 1.755 ± 0.014	3.145ValAsp: 3.145 ± 0.013	3.981ValGlu: 3.981 ± 0.018	2.77ValPhe: 2.77 ± 0.014	3.553ValGly: 3.553 ± 0.017	1.584ValHis: 1.584 ± 0.009	2.994ValIle: 2.994 ± 0.017	3.585ValLys: 3.585 ± 0.016	6.278ValLeu: 6.278 ± 0.025	1.513ValMet: 1.513 ± 0.01	2.443ValAsn: 2.443 ± 0.016	3.218ValPro: 3.218 ± 0.014	2.777ValGln: 2.777 ± 0.016	3.264ValArg: 3.264 ± 0.015	5.384ValSer: 5.384 ± 0.019	3.894ValThr: 3.894 ± 0.017	4.333ValVal: 4.333 ± 0.02	0.77ValTrp: 0.77 ± 0.007	1.79ValTyr: 1.79 ± 0.01	0.028ValXaa: 0.028 ± 0.001
Trp	0.657TrpAla: 0.657 ± 0.006	0.251TrpCys: 0.251 ± 0.005	0.643TrpAsp: 0.643 ± 0.006	0.738TrpGlu: 0.738 ± 0.006	0.484TrpPhe: 0.484 ± 0.005	0.615TrpGly: 0.615 ± 0.007	0.278TrpHis: 0.278 ± 0.004	0.589TrpIle: 0.589 ± 0.007	0.734TrpLys: 0.734 ± 0.006	1.159TrpLeu: 1.159 ± 0.009	0.346TrpMet: 0.346 ± 0.004	0.511TrpAsn: 0.511 ± 0.006	0.436TrpPro: 0.436 ± 0.004	0.47TrpGln: 0.47 ± 0.006	0.76TrpArg: 0.76 ± 0.007	0.98TrpSer: 0.98 ± 0.008	0.724TrpThr: 0.724 ± 0.006	0.7TrpVal: 0.7 ± 0.007	0.195TrpTrp: 0.195 ± 0.003	0.34TrpTyr: 0.34 ± 0.005	0.004TrpXaa: 0.004 ± 0.0
Tyr	1.412TyrAla: 1.412 ± 0.01	0.715TyrCys: 0.715 ± 0.006	1.363TyrAsp: 1.363 ± 0.011	1.545TyrGlu: 1.545 ± 0.009	1.232TyrPhe: 1.232 ± 0.009	1.667TyrGly: 1.667 ± 0.011	0.779TyrHis: 0.779 ± 0.007	1.452TyrIle: 1.452 ± 0.011	1.483TyrLys: 1.483 ± 0.011	2.633TyrLeu: 2.633 ± 0.013	0.669TyrMet: 0.669 ± 0.006	1.19TyrAsn: 1.19 ± 0.008	1.274TyrPro: 1.274 ± 0.009	1.213TyrGln: 1.213 ± 0.007	1.69TyrArg: 1.69 ± 0.011	2.351TyrSer: 2.351 ± 0.013	1.546TyrThr: 1.546 ± 0.01	1.62TyrVal: 1.62 ± 0.011	0.376TyrTrp: 0.376 ± 0.006	0.983TyrTyr: 0.983 ± 0.009	0.013TyrXaa: 0.013 ± 0.001
Xaa	0.026XaaAla: 0.026 ± 0.001	0.012XaaCys: 0.012 ± 0.001	0.024XaaAsp: 0.024 ± 0.001	0.027XaaGlu: 0.027 ± 0.001	0.021XaaPhe: 0.021 ± 0.001	0.028XaaGly: 0.028 ± 0.001	0.014XaaHis: 0.014 ± 0.001	0.02XaaIle: 0.02 ± 0.001	0.028XaaLys: 0.028 ± 0.001	0.043XaaLeu: 0.043 ± 0.002	0.015XaaMet: 0.015 ± 0.001	0.02XaaAsn: 0.02 ± 0.001	0.028XaaPro: 0.028 ± 0.001	0.023XaaGln: 0.023 ± 0.001	0.023XaaArg: 0.023 ± 0.001	0.045XaaSer: 0.045 ± 0.001	0.025XaaThr: 0.025 ± 0.001	0.031XaaVal: 0.031 ± 0.001	0.006XaaTrp: 0.006 ± 0.001	0.013XaaTyr: 0.013 ± 0.001	0.535XaaXaa: 0.535 ± 0.068

Statistics based on 33944 proteins (19349097 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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