Macaca mulatta feces associated virus 4
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W5Q0A5|A0A1W5Q0A5_9VIRU Rep OS=Macaca mulatta feces associated virus 4 OX=2499226 PE=4 SV=1
MM1 pKa = 7.64 LPEE4 pKa = 4.53 GLTAEE9 pKa = 4.39 GTIEE13 pKa = 3.98 GDD15 pKa = 3.03 RR16 pKa = 11.84 VVSVRR21 pKa = 11.84 VSEE24 pKa = 4.47 TYY26 pKa = 10.7 DD27 pKa = 3.52 LSTQVGKK34 pKa = 9.88 MGIVGIHH41 pKa = 5.32 TPIGTLVEE49 pKa = 4.4 RR50 pKa = 11.84 MWSGLVRR57 pKa = 11.84 QYY59 pKa = 11.8 GKK61 pKa = 10.68 FRR63 pKa = 11.84 FKK65 pKa = 11.02 SCDD68 pKa = 3.09 VAMACASMLPADD80 pKa = 4.26 PLQIGVEE87 pKa = 4.06 AGAIAPQDD95 pKa = 3.54 MFNPILYY102 pKa = 9.84 KK103 pKa = 10.59 AVSNDD108 pKa = 3.03 TMNTFLQYY116 pKa = 10.4 IQGQAIFNTGSTSGKK131 pKa = 8.76 IVNFGSVIGVNDD143 pKa = 4.11 SDD145 pKa = 4.19 FKK147 pKa = 11.03 TPGDD151 pKa = 3.93 APTSIDD157 pKa = 3.6 QFQMYY162 pKa = 10.03 YY163 pKa = 11.12 GLLSDD168 pKa = 3.82 PSGWRR173 pKa = 11.84 KK174 pKa = 9.98 AMPQSGLVMRR184 pKa = 11.84 GLRR187 pKa = 11.84 PLVYY191 pKa = 10.35 NVVSSYY197 pKa = 11.31 GLNEE201 pKa = 3.73 ASGAGVTGAPKK212 pKa = 10.53 NDD214 pKa = 2.85 IKK216 pKa = 11.37 SNQFIGDD223 pKa = 3.55 TRR225 pKa = 11.84 YY226 pKa = 7.41 TAAGARR232 pKa = 11.84 AATNGSDD239 pKa = 3.32 AQLPDD244 pKa = 3.84 VVSGGQNVYY253 pKa = 10.51 QDD255 pKa = 3.65 NPVFGSFKK263 pKa = 10.54 GRR265 pKa = 11.84 SQPMPWINTKK275 pKa = 9.73 FWPSAVGNSDD285 pKa = 2.46 GSMITEE291 pKa = 4.03 TEE293 pKa = 4.22 STNKK297 pKa = 10.36 DD298 pKa = 2.69 ATLQSNVGRR307 pKa = 11.84 VPPAYY312 pKa = 10.09 VGLIVLPPAKK322 pKa = 10.23 LNQLYY327 pKa = 10.29 YY328 pKa = 10.16 RR329 pKa = 11.84 IKK331 pKa = 9.09 VTWTIEE337 pKa = 3.97 FSEE340 pKa = 4.45 LQSNLAVSNWQGIAKK355 pKa = 10.11 FGSLSYY361 pKa = 9.92 ATDD364 pKa = 3.31 YY365 pKa = 11.21 AAQSKK370 pKa = 10.99 SMDD373 pKa = 4.26 SIAGMVDD380 pKa = 2.87 TSDD383 pKa = 4.03 ADD385 pKa = 3.47 VEE387 pKa = 4.59 KK388 pKa = 10.81 IMEE391 pKa = 4.41 GAA393 pKa = 3.49
Molecular weight: 42.13 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.9
IPC2_protein 4.991
IPC_protein 4.902
Toseland 4.736
ProMoST 5.029
Dawson 4.863
Bjellqvist 5.016
Wikipedia 4.762
Rodwell 4.736
Grimsley 4.647
Solomon 4.863
Lehninger 4.813
Nozaki 4.965
DTASelect 5.194
Thurlkill 4.749
EMBOSS 4.774
Sillero 5.016
Patrickios 3.986
IPC_peptide 4.863
IPC2_peptide 5.003
IPC2.peptide.svr19 5.063
Protein with the highest isoelectric point:
>tr|A0A1W5Q0A5|A0A1W5Q0A5_9VIRU Rep OS=Macaca mulatta feces associated virus 4 OX=2499226 PE=4 SV=1
MM1 pKa = 7.4 RR2 pKa = 11.84 RR3 pKa = 11.84 IRR5 pKa = 11.84 EE6 pKa = 4.39 TMGKK10 pKa = 8.04 TYY12 pKa = 10.37 MLTIPRR18 pKa = 11.84 NDD20 pKa = 3.42 TYY22 pKa = 11.52 RR23 pKa = 11.84 EE24 pKa = 4.14 GNKK27 pKa = 9.27 IFAWFRR33 pKa = 11.84 KK34 pKa = 9.35 NDD36 pKa = 2.9 IHH38 pKa = 7.66 KK39 pKa = 9.66 WVLGAEE45 pKa = 4.32 KK46 pKa = 10.65 GSGGYY51 pKa = 7.21 EE52 pKa = 3.54 HH53 pKa = 6.86 WQMRR57 pKa = 11.84 VQCRR61 pKa = 11.84 FGFEE65 pKa = 3.92 EE66 pKa = 4.85 LKK68 pKa = 11.12 VLFPTAHH75 pKa = 6.85 IEE77 pKa = 4.06 EE78 pKa = 5.34 CSDD81 pKa = 2.43 KK82 pKa = 9.17 WTYY85 pKa = 7.48 EE86 pKa = 3.98 AKK88 pKa = 10.63 EE89 pKa = 3.96 GVYY92 pKa = 9.06 WRR94 pKa = 11.84 SNDD97 pKa = 2.95 RR98 pKa = 11.84 RR99 pKa = 11.84 EE100 pKa = 4.38 NIQQRR105 pKa = 11.84 FGKK108 pKa = 9.99 LRR110 pKa = 11.84 YY111 pKa = 7.83 AQKK114 pKa = 10.56 RR115 pKa = 11.84 VIEE118 pKa = 4.38 RR119 pKa = 11.84 ADD121 pKa = 3.38 GTNDD125 pKa = 3.28 RR126 pKa = 11.84 EE127 pKa = 4.38 VVVWYY132 pKa = 9.72 DD133 pKa = 3.37 QEE135 pKa = 4.44 GKK137 pKa = 10.31 AGKK140 pKa = 9.48 SWLTGAMWEE149 pKa = 4.15 RR150 pKa = 11.84 GLAYY154 pKa = 10.13 FSVADD159 pKa = 3.9 TSGKK163 pKa = 8.42 TIVMDD168 pKa = 4.01 IASEE172 pKa = 4.03 YY173 pKa = 10.46 LKK175 pKa = 11.06 NGWRR179 pKa = 11.84 PYY181 pKa = 10.32 IIIDD185 pKa = 3.45 IPRR188 pKa = 11.84 AGKK191 pKa = 6.86 WTPEE195 pKa = 3.79 LYY197 pKa = 10.39 EE198 pKa = 4.67 AIEE201 pKa = 4.45 RR202 pKa = 11.84 IKK204 pKa = 11.02 DD205 pKa = 3.65 GLIKK209 pKa = 10.41 DD210 pKa = 3.72 PRR212 pKa = 11.84 YY213 pKa = 10.31 SSEE216 pKa = 3.54 AVNIHH221 pKa = 4.8 GVKK224 pKa = 10.15 VIVMTNTMPKK234 pKa = 9.79 LDD236 pKa = 4.46 KK237 pKa = 11.01 LSADD241 pKa = 2.96 RR242 pKa = 11.84 WKK244 pKa = 10.6 IITQQEE250 pKa = 4.09 LTLEE254 pKa = 4.13 EE255 pKa = 3.89 TAIEE259 pKa = 4.08 QFALTGKK266 pKa = 10.13 GGYY269 pKa = 9.12 RR270 pKa = 11.84 PPLTPPTWDD279 pKa = 3.28 SRR281 pKa = 11.84 DD282 pKa = 3.3 VRR284 pKa = 11.84 GPLSS288 pKa = 3.28
Molecular weight: 33.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.629
IPC2_protein 8.565
IPC_protein 8.507
Toseland 9.136
ProMoST 8.96
Dawson 9.443
Bjellqvist 9.194
Wikipedia 9.633
Rodwell 9.633
Grimsley 9.516
Solomon 9.502
Lehninger 9.458
Nozaki 9.224
DTASelect 9.151
Thurlkill 9.282
EMBOSS 9.575
Sillero 9.399
Patrickios 4.813
IPC_peptide 9.502
IPC2_peptide 7.761
IPC2.peptide.svr19 7.663
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
681
288
393
340.5
37.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.342 ± 0.925
0.587 ± 0.069
5.727 ± 0.11
5.433 ± 1.864
3.231 ± 0.291
9.251 ± 0.813
0.734 ± 0.421
6.314 ± 0.628
5.727 ± 1.229
6.021 ± 0.076
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.377 ± 0.385
4.258 ± 0.728
4.846 ± 0.436
4.258 ± 0.728
5.58 ± 1.992
7.195 ± 1.946
6.461 ± 0.087
6.902 ± 1.088
2.643 ± 0.979
4.112 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here