Enhydrobacter sp. CC-CFT640

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillales incertae sedis; Enhydrobacter; unclassified Enhydrobacter

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7888 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C8PBN7|A0A5C8PBN7_9PROT Uncharacterized protein OS=Enhydrobacter sp. CC-CFT640 OX=2586908 GN=FHP25_32560 PE=4 SV=1
MM1 pKa = 7.56AGDD4 pKa = 4.68GSTGAPGGTPQVPHH18 pKa = 7.24LLDD21 pKa = 4.74GYY23 pKa = 10.23AVRR26 pKa = 11.84PAWEE30 pKa = 4.13VAGVDD35 pKa = 3.87YY36 pKa = 11.08AVGVHH41 pKa = 6.3PGITLRR47 pKa = 11.84VPTADD52 pKa = 3.75NLPAGTTLGNHH63 pKa = 6.55VIYY66 pKa = 10.63INGTDD71 pKa = 3.61VTLSGYY77 pKa = 10.7NLTNYY82 pKa = 7.95TVLVTATAKK91 pKa = 9.21GTATIVDD98 pKa = 4.39CAATTGINIRR108 pKa = 11.84STVDD112 pKa = 2.8ATANLVVRR120 pKa = 11.84YY121 pKa = 9.65CSFDD125 pKa = 3.39GGGMASDD132 pKa = 4.81PDD134 pKa = 3.89FSLIKK139 pKa = 9.66VWCPLTVEE147 pKa = 4.01YY148 pKa = 10.73SVLKK152 pKa = 10.07NAPAAIYY159 pKa = 9.99APEE162 pKa = 4.73PMTVMYY168 pKa = 11.09NVMSGFAWQDD178 pKa = 3.06GAHH181 pKa = 6.46ACAIAVEE188 pKa = 4.68GTNDD192 pKa = 3.5PNASALIAYY201 pKa = 7.18NTIYY205 pKa = 10.93SGDD208 pKa = 3.37ARR210 pKa = 11.84NAEE213 pKa = 4.28GFPIGIGTGIAFYY226 pKa = 11.17NDD228 pKa = 3.0AGGNFYY234 pKa = 11.17NSTVANNTVIANLPGGASYY253 pKa = 9.08LTGFYY258 pKa = 10.57VDD260 pKa = 4.25PPGTATNMHH269 pKa = 5.74VQDD272 pKa = 3.76NFYY275 pKa = 11.34ASVNGFNNASSGAYY289 pKa = 7.87GALYY293 pKa = 10.02IGTKK297 pKa = 9.24GTVQATYY304 pKa = 10.2TNNIDD309 pKa = 3.37MSTGQLVAGSDD320 pKa = 3.75AGPVDD325 pKa = 3.67TGTVQSDD332 pKa = 3.22VSYY335 pKa = 9.15TLPAGIEE342 pKa = 4.05NLTLTGSDD350 pKa = 4.02NINGTGNSLANVLTGNAGNNILDD373 pKa = 3.92GGANADD379 pKa = 3.91HH380 pKa = 6.61MAGGAGDD387 pKa = 3.72DD388 pKa = 3.98TYY390 pKa = 11.99VVDD393 pKa = 3.67NAGDD397 pKa = 3.74VVVEE401 pKa = 4.27EE402 pKa = 4.63NGQGTDD408 pKa = 3.32TVLSSVSYY416 pKa = 11.05SLANYY421 pKa = 9.66IEE423 pKa = 4.29NLTLTGNGNINGTGNSLANVLTGNSGSNTLNGGLGADD460 pKa = 3.89TLNGGGGNDD469 pKa = 3.93TLNGGAGNDD478 pKa = 3.99TLNGGAGNDD487 pKa = 3.56TAVYY491 pKa = 9.9AGNRR495 pKa = 11.84ADD497 pKa = 4.07YY498 pKa = 10.35EE499 pKa = 4.08ILAVGGGLTIRR510 pKa = 11.84DD511 pKa = 4.06LNPANGDD518 pKa = 3.55EE519 pKa = 4.68GTDD522 pKa = 3.41TLQGIEE528 pKa = 4.22MLRR531 pKa = 11.84FADD534 pKa = 3.29ITVPASTSNAAPVAGDD550 pKa = 3.84DD551 pKa = 3.94SASGSEE557 pKa = 4.42DD558 pKa = 3.21KK559 pKa = 11.1AITTGNVLANDD570 pKa = 3.69SDD572 pKa = 4.32ADD574 pKa = 3.93STLTAASIIGFSQGAHH590 pKa = 5.52GAVVYY595 pKa = 10.61NGNGTFTYY603 pKa = 10.29TPVPNYY609 pKa = 10.57NGADD613 pKa = 3.25SFTYY617 pKa = 9.56TVSDD621 pKa = 3.31GHH623 pKa = 6.5GGSDD627 pKa = 3.42TARR630 pKa = 11.84VSITVNPVNDD640 pKa = 3.9APVAIDD646 pKa = 5.18DD647 pKa = 5.07GPLQATGDD655 pKa = 3.73QPLVISAAALLANDD669 pKa = 3.99TDD671 pKa = 4.29ADD673 pKa = 4.27LDD675 pKa = 3.77ALTIQGVTQPAHH687 pKa = 6.41GALADD692 pKa = 3.83NGNGTFTYY700 pKa = 10.27TPVRR704 pKa = 11.84NYY706 pKa = 10.89NGADD710 pKa = 3.02SFTYY714 pKa = 9.56TVSDD718 pKa = 3.31GHH720 pKa = 6.5GGSDD724 pKa = 3.42TARR727 pKa = 11.84VSIAVNPVNDD737 pKa = 4.07APVAIDD743 pKa = 5.18DD744 pKa = 5.07GPLQATGDD752 pKa = 3.73QPLVISAAALLANDD766 pKa = 3.82TDD768 pKa = 4.29ADD770 pKa = 3.92FDD772 pKa = 4.15ALTIQGVTQPTHH784 pKa = 5.42GTLADD789 pKa = 3.72NGNGTFTYY797 pKa = 10.0TRR799 pKa = 11.84DD800 pKa = 3.35AGYY803 pKa = 10.92VGADD807 pKa = 2.98SFTYY811 pKa = 10.13TVDD814 pKa = 3.88DD815 pKa = 3.85GHH817 pKa = 6.8GGSDD821 pKa = 3.2TATVSIRR828 pKa = 11.84VDD830 pKa = 3.34DD831 pKa = 4.07AAGRR835 pKa = 11.84TLTGNEE841 pKa = 3.69RR842 pKa = 11.84ANYY845 pKa = 9.76LVGGAGNDD853 pKa = 3.71TLNGRR858 pKa = 11.84GGNDD862 pKa = 3.27TLNGRR867 pKa = 11.84GGNDD871 pKa = 3.28TLNGGGGNDD880 pKa = 3.81TLNGGDD886 pKa = 5.4GDD888 pKa = 4.04DD889 pKa = 4.33TLNGGAGNDD898 pKa = 3.59TLLGGRR904 pKa = 11.84GRR906 pKa = 11.84DD907 pKa = 3.64VLIGGAGADD916 pKa = 3.81LFDD919 pKa = 3.66FHH921 pKa = 8.18AFNEE925 pKa = 4.52SGTTDD930 pKa = 3.06ATRR933 pKa = 11.84DD934 pKa = 3.49QIVGFEE940 pKa = 4.14RR941 pKa = 11.84GTDD944 pKa = 3.56RR945 pKa = 11.84IDD947 pKa = 4.27LATIDD952 pKa = 4.63ANTAAAGNQAFAFIGGAAFHH972 pKa = 6.39GVAGEE977 pKa = 4.12LRR979 pKa = 11.84QQAFGADD986 pKa = 3.83TIVSGDD992 pKa = 3.58VNGDD996 pKa = 3.25AVADD1000 pKa = 3.94FQIQLKK1006 pKa = 10.4GAFALTASDD1015 pKa = 4.73FFLL1018 pKa = 5.94

Molecular weight: 102.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C8P6U6|A0A5C8P6U6_9PROT Adenylate/guanylate cyclase domain-containing protein OS=Enhydrobacter sp. CC-CFT640 OX=2586908 GN=FHP25_39895 PE=4 SV=1
MM1 pKa = 7.87AKK3 pKa = 8.84RR4 pKa = 11.84TTKK7 pKa = 10.6SAVARR12 pKa = 11.84TKK14 pKa = 8.25TAKK17 pKa = 10.37KK18 pKa = 9.33KK19 pKa = 9.62VEE21 pKa = 4.07RR22 pKa = 11.84TTVTTKK28 pKa = 10.42RR29 pKa = 11.84RR30 pKa = 11.84TAAKK34 pKa = 9.82AKK36 pKa = 10.0VKK38 pKa = 10.77AKK40 pKa = 10.78VKK42 pKa = 10.64VKK44 pKa = 10.33VKK46 pKa = 10.28KK47 pKa = 9.25AAKK50 pKa = 9.57RR51 pKa = 11.84VAPKK55 pKa = 10.24AAAKK59 pKa = 9.83SARR62 pKa = 11.84AGTAVKK68 pKa = 10.37RR69 pKa = 11.84PAAKK73 pKa = 9.98KK74 pKa = 9.59RR75 pKa = 11.84VAAKK79 pKa = 10.12RR80 pKa = 11.84ATSKK84 pKa = 9.36TRR86 pKa = 11.84ASRR89 pKa = 3.35

Molecular weight: 9.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7888

0

7888

2550868

26

5084

323.4

34.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.444 ± 0.047

0.932 ± 0.01

5.696 ± 0.026

4.838 ± 0.026

3.477 ± 0.017

8.82 ± 0.035

2.138 ± 0.014

4.731 ± 0.02

2.825 ± 0.021

10.112 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.013

2.253 ± 0.015

5.682 ± 0.026

3.145 ± 0.013

7.931 ± 0.033

4.841 ± 0.021

5.214 ± 0.029

7.72 ± 0.028

1.513 ± 0.013

2.215 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski