Amino acid dipepetide frequency for Enhydrobacter sp. CC-CFT640

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.655AlaAla: 20.655 ± 0.154	1.338AlaCys: 1.338 ± 0.026	7.421AlaAsp: 7.421 ± 0.06	6.709AlaGlu: 6.709 ± 0.057	4.535AlaPhe: 4.535 ± 0.048	12.065AlaGly: 12.065 ± 0.095	2.539AlaHis: 2.539 ± 0.033	6.193AlaIle: 6.193 ± 0.056	3.301AlaLys: 3.301 ± 0.045	14.313AlaLeu: 14.313 ± 0.091	3.765AlaMet: 3.765 ± 0.044	2.619AlaAsn: 2.619 ± 0.038	6.662AlaPro: 6.662 ± 0.073	4.429AlaGln: 4.429 ± 0.054	10.572AlaArg: 10.572 ± 0.093	6.069AlaSer: 6.069 ± 0.059	6.519AlaThr: 6.519 ± 0.057	10.004AlaVal: 10.004 ± 0.079	1.999AlaTrp: 1.999 ± 0.025	2.734AlaTyr: 2.734 ± 0.029	0.0AlaXaa: 0.0 ± 0.0
Cys	1.17CysAla: 1.17 ± 0.026	0.142CysCys: 0.142 ± 0.008	0.586CysAsp: 0.586 ± 0.015	0.395CysGlu: 0.395 ± 0.013	0.333CysPhe: 0.333 ± 0.01	1.042CysGly: 1.042 ± 0.02	0.269CysHis: 0.269 ± 0.012	0.382CysIle: 0.382 ± 0.013	0.198CysLys: 0.198 ± 0.01	0.858CysLeu: 0.858 ± 0.017	0.177CysMet: 0.177 ± 0.009	0.22CysAsn: 0.22 ± 0.009	0.515CysPro: 0.515 ± 0.014	0.248CysGln: 0.248 ± 0.011	0.814CysArg: 0.814 ± 0.019	0.446CysSer: 0.446 ± 0.012	0.469CysThr: 0.469 ± 0.015	0.7CysVal: 0.7 ± 0.015	0.149CysTrp: 0.149 ± 0.008	0.204CysTyr: 0.204 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	7.343AspAla: 7.343 ± 0.063	0.5AspCys: 0.5 ± 0.014	3.229AspAsp: 3.229 ± 0.044	2.845AspGlu: 2.845 ± 0.036	1.977AspPhe: 1.977 ± 0.029	5.535AspGly: 5.535 ± 0.058	1.238AspHis: 1.238 ± 0.024	2.865AspIle: 2.865 ± 0.035	1.642AspLys: 1.642 ± 0.028	5.646AspLeu: 5.646 ± 0.05	1.339AspMet: 1.339 ± 0.024	1.19AspAsn: 1.19 ± 0.022	3.704AspPro: 3.704 ± 0.042	1.561AspGln: 1.561 ± 0.025	4.576AspArg: 4.576 ± 0.046	2.167AspSer: 2.167 ± 0.03	2.607AspThr: 2.607 ± 0.04	4.499AspVal: 4.499 ± 0.043	1.049AspTrp: 1.049 ± 0.023	1.445AspTyr: 1.445 ± 0.027	0.0AspXaa: 0.0 ± 0.0
Glu	7.088GluAla: 7.088 ± 0.055	0.312GluCys: 0.312 ± 0.014	2.176GluAsp: 2.176 ± 0.032	2.238GluGlu: 2.238 ± 0.037	1.398GluPhe: 1.398 ± 0.022	3.516GluGly: 3.516 ± 0.039	1.161GluHis: 1.161 ± 0.023	2.782GluIle: 2.782 ± 0.035	1.701GluLys: 1.701 ± 0.032	4.83GluLeu: 4.83 ± 0.049	1.316GluMet: 1.316 ± 0.022	1.086GluAsn: 1.086 ± 0.022	2.546GluPro: 2.546 ± 0.039	1.893GluGln: 1.893 ± 0.031	4.793GluArg: 4.793 ± 0.053	1.968GluSer: 1.968 ± 0.029	2.652GluThr: 2.652 ± 0.029	3.427GluVal: 3.427 ± 0.042	0.629GluTrp: 0.629 ± 0.016	0.862GluTyr: 0.862 ± 0.021	0.0GluXaa: 0.0 ± 0.0
Phe	4.386PheAla: 4.386 ± 0.042	0.407PheCys: 0.407 ± 0.013	2.49PheAsp: 2.49 ± 0.031	1.795PheGlu: 1.795 ± 0.028	1.248PhePhe: 1.248 ± 0.025	3.539PheGly: 3.539 ± 0.043	0.809PheHis: 0.809 ± 0.016	1.479PheIle: 1.479 ± 0.026	0.92PheLys: 0.92 ± 0.021	3.124PheLeu: 3.124 ± 0.04	0.776PheMet: 0.776 ± 0.018	1.046PheAsn: 1.046 ± 0.023	1.608PhePro: 1.608 ± 0.027	0.937PheGln: 0.937 ± 0.02	2.319PheArg: 2.319 ± 0.031	1.794PheSer: 1.794 ± 0.027	1.935PheThr: 1.935 ± 0.031	2.793PheVal: 2.793 ± 0.035	0.555PheTrp: 0.555 ± 0.016	0.81PheTyr: 0.81 ± 0.019	0.0PheXaa: 0.0 ± 0.0
Gly	10.228GlyAla: 10.228 ± 0.092	0.909GlyCys: 0.909 ± 0.019	4.505GlyAsp: 4.505 ± 0.049	4.138GlyGlu: 4.138 ± 0.04	3.461GlyPhe: 3.461 ± 0.041	8.048GlyGly: 8.048 ± 0.104	1.937GlyHis: 1.937 ± 0.029	4.512GlyIle: 4.512 ± 0.046	3.017GlyLys: 3.017 ± 0.039	8.949GlyLeu: 8.949 ± 0.065	2.387GlyMet: 2.387 ± 0.03	1.997GlyAsn: 1.997 ± 0.042	3.975GlyPro: 3.975 ± 0.041	2.815GlyGln: 2.815 ± 0.036	7.127GlyArg: 7.127 ± 0.057	4.592GlySer: 4.592 ± 0.061	5.013GlyThr: 5.013 ± 0.089	6.619GlyVal: 6.619 ± 0.064	1.608GlyTrp: 1.608 ± 0.026	2.367GlyTyr: 2.367 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.741HisAla: 2.741 ± 0.038	0.234HisCys: 0.234 ± 0.009	1.324HisAsp: 1.324 ± 0.024	0.97HisGlu: 0.97 ± 0.02	0.795HisPhe: 0.795 ± 0.019	2.185HisGly: 2.185 ± 0.031	0.623HisHis: 0.623 ± 0.018	0.884HisIle: 0.884 ± 0.019	0.481HisLys: 0.481 ± 0.013	2.107HisLeu: 2.107 ± 0.032	0.495HisMet: 0.495 ± 0.015	0.465HisAsn: 0.465 ± 0.013	1.465HisPro: 1.465 ± 0.028	0.592HisGln: 0.592 ± 0.016	1.691HisArg: 1.691 ± 0.027	0.764HisSer: 0.764 ± 0.016	0.895HisThr: 0.895 ± 0.017	1.746HisVal: 1.746 ± 0.023	0.362HisTrp: 0.362 ± 0.011	0.561HisTyr: 0.561 ± 0.014	0.0HisXaa: 0.0 ± 0.0
Ile	7.31IleAla: 7.31 ± 0.056	0.505IleCys: 0.505 ± 0.013	3.532IleAsp: 3.532 ± 0.041	3.021IleGlu: 3.021 ± 0.04	1.439IlePhe: 1.439 ± 0.03	4.883IleGly: 4.883 ± 0.051	0.824IleHis: 0.824 ± 0.018	1.849IleIle: 1.849 ± 0.029	1.254IleLys: 1.254 ± 0.024	3.793IleLeu: 3.793 ± 0.042	0.88IleMet: 0.88 ± 0.021	1.248IleAsn: 1.248 ± 0.027	2.03IlePro: 2.03 ± 0.028	1.133IleGln: 1.133 ± 0.02	3.054IleArg: 3.054 ± 0.037	2.166IleSer: 2.166 ± 0.034	2.521IleThr: 2.521 ± 0.039	4.216IleVal: 4.216 ± 0.048	0.577IleTrp: 0.577 ± 0.016	1.073IleTyr: 1.073 ± 0.025	0.0IleXaa: 0.0 ± 0.0
Lys	3.908LysAla: 3.908 ± 0.047	0.153LysCys: 0.153 ± 0.007	1.565LysAsp: 1.565 ± 0.03	1.26LysGlu: 1.26 ± 0.03	0.763LysPhe: 0.763 ± 0.02	2.311LysGly: 2.311 ± 0.034	0.541LysHis: 0.541 ± 0.014	1.368LysIle: 1.368 ± 0.027	1.029LysLys: 1.029 ± 0.026	2.842LysLeu: 2.842 ± 0.041	0.679LysMet: 0.679 ± 0.018	0.631LysAsn: 0.631 ± 0.017	1.938LysPro: 1.938 ± 0.032	0.852LysGln: 0.852 ± 0.021	2.16LysArg: 2.16 ± 0.029	1.363LysSer: 1.363 ± 0.025	1.599LysThr: 1.599 ± 0.029	2.338LysVal: 2.338 ± 0.033	0.376LysTrp: 0.376 ± 0.012	0.58LysTyr: 0.58 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	14.934LeuAla: 14.934 ± 0.09	0.951LeuCys: 0.951 ± 0.019	6.039LeuAsp: 6.039 ± 0.057	4.681LeuGlu: 4.681 ± 0.048	3.308LeuPhe: 3.308 ± 0.038	8.63LeuGly: 8.63 ± 0.074	1.987LeuHis: 1.987 ± 0.031	4.42LeuIle: 4.42 ± 0.047	3.165LeuLys: 3.165 ± 0.042	9.947LeuLeu: 9.947 ± 0.098	2.342LeuMet: 2.342 ± 0.031	2.175LeuAsn: 2.175 ± 0.029	5.98LeuPro: 5.98 ± 0.057	3.023LeuGln: 3.023 ± 0.033	7.885LeuArg: 7.885 ± 0.069	5.536LeuSer: 5.536 ± 0.053	5.057LeuThr: 5.057 ± 0.05	7.726LeuVal: 7.726 ± 0.066	1.327LeuTrp: 1.327 ± 0.023	2.003LeuTyr: 2.003 ± 0.031	0.0LeuXaa: 0.0 ± 0.0
Met	3.457MetAla: 3.457 ± 0.037	0.198MetCys: 0.198 ± 0.008	1.069MetAsp: 1.069 ± 0.02	0.961MetGlu: 0.961 ± 0.018	0.697MetPhe: 0.697 ± 0.016	1.789MetGly: 1.789 ± 0.031	0.465MetHis: 0.465 ± 0.012	1.157MetIle: 1.157 ± 0.023	0.854MetLys: 0.854 ± 0.017	2.554MetLeu: 2.554 ± 0.034	0.664MetMet: 0.664 ± 0.015	0.605MetAsn: 0.605 ± 0.014	1.685MetPro: 1.685 ± 0.028	0.809MetGln: 0.809 ± 0.021	1.986MetArg: 1.986 ± 0.03	1.552MetSer: 1.552 ± 0.029	1.814MetThr: 1.814 ± 0.025	1.805MetVal: 1.805 ± 0.03	0.245MetTrp: 0.245 ± 0.01	0.374MetTyr: 0.374 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.907AsnAla: 2.907 ± 0.05	0.231AsnCys: 0.231 ± 0.009	1.246AsnAsp: 1.246 ± 0.025	0.959AsnGlu: 0.959 ± 0.02	0.777AsnPhe: 0.777 ± 0.019	2.169AsnGly: 2.169 ± 0.039	0.448AsnHis: 0.448 ± 0.014	1.092AsnIle: 1.092 ± 0.022	0.596AsnLys: 0.596 ± 0.014	2.271AsnLeu: 2.271 ± 0.031	0.522AsnMet: 0.522 ± 0.014	0.589AsnAsn: 0.589 ± 0.018	1.673AsnPro: 1.673 ± 0.028	0.63AsnGln: 0.63 ± 0.018	1.683AsnArg: 1.683 ± 0.024	0.884AsnSer: 0.884 ± 0.022	1.098AsnThr: 1.098 ± 0.025	1.844AsnVal: 1.844 ± 0.031	0.351AsnTrp: 0.351 ± 0.011	0.557AsnTyr: 0.557 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	7.708ProAla: 7.708 ± 0.079	0.385ProCys: 0.385 ± 0.013	3.897ProAsp: 3.897 ± 0.041	3.121ProGlu: 3.121 ± 0.04	1.938ProPhe: 1.938 ± 0.027	5.21ProGly: 5.21 ± 0.053	1.255ProHis: 1.255 ± 0.024	2.309ProIle: 2.309 ± 0.03	1.479ProLys: 1.479 ± 0.03	5.093ProLeu: 5.093 ± 0.052	1.327ProMet: 1.327 ± 0.024	1.2ProAsn: 1.2 ± 0.023	3.853ProPro: 3.853 ± 0.084	1.951ProGln: 1.951 ± 0.027	3.765ProArg: 3.765 ± 0.043	2.943ProSer: 2.943 ± 0.036	2.82ProThr: 2.82 ± 0.029	4.351ProVal: 4.351 ± 0.047	0.902ProTrp: 0.902 ± 0.021	1.314ProTyr: 1.314 ± 0.027	0.0ProXaa: 0.0 ± 0.0
Gln	4.649GlnAla: 4.649 ± 0.043	0.234GlnCys: 0.234 ± 0.01	1.458GlnAsp: 1.458 ± 0.025	1.299GlnGlu: 1.299 ± 0.026	0.967GlnPhe: 0.967 ± 0.019	2.45GlnGly: 2.45 ± 0.033	0.738GlnHis: 0.738 ± 0.017	1.577GlnIle: 1.577 ± 0.023	0.906GlnLys: 0.906 ± 0.021	2.875GlnLeu: 2.875 ± 0.035	0.776GlnMet: 0.776 ± 0.017	0.7GlnAsn: 0.7 ± 0.016	2.007GlnPro: 2.007 ± 0.029	1.283GlnGln: 1.283 ± 0.026	2.998GlnArg: 2.998 ± 0.037	1.518GlnSer: 1.518 ± 0.028	1.531GlnThr: 1.531 ± 0.023	2.44GlnVal: 2.44 ± 0.035	0.454GlnTrp: 0.454 ± 0.014	0.588GlnTyr: 0.588 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	9.343ArgAla: 9.343 ± 0.075	0.752ArgCys: 0.752 ± 0.019	4.627ArgAsp: 4.627 ± 0.054	3.897ArgGlu: 3.897 ± 0.046	3.073ArgPhe: 3.073 ± 0.035	5.319ArgGly: 5.319 ± 0.046	2.115ArgHis: 2.115 ± 0.03	4.081ArgIle: 4.081 ± 0.04	2.191ArgLys: 2.191 ± 0.032	9.068ArgLeu: 9.068 ± 0.074	2.017ArgMet: 2.017 ± 0.026	1.767ArgAsn: 1.767 ± 0.028	4.431ArgPro: 4.431 ± 0.05	3.07ArgGln: 3.07 ± 0.034	7.633ArgArg: 7.633 ± 0.07	3.678ArgSer: 3.678 ± 0.041	3.85ArgThr: 3.85 ± 0.041	5.246ArgVal: 5.246 ± 0.053	1.273ArgTrp: 1.273 ± 0.023	1.876ArgTyr: 1.876 ± 0.027	0.0ArgXaa: 0.0 ± 0.0
Ser	5.756SerAla: 5.756 ± 0.057	0.464SerCys: 0.464 ± 0.013	2.639SerAsp: 2.639 ± 0.032	2.044SerGlu: 2.044 ± 0.031	2.007SerPhe: 2.007 ± 0.024	5.195SerGly: 5.195 ± 0.066	0.972SerHis: 0.972 ± 0.02	2.368SerIle: 2.368 ± 0.036	1.19SerLys: 1.19 ± 0.025	4.978SerLeu: 4.978 ± 0.048	1.221SerMet: 1.221 ± 0.022	1.086SerAsn: 1.086 ± 0.022	2.826SerPro: 2.826 ± 0.036	1.322SerGln: 1.322 ± 0.024	3.576SerArg: 3.576 ± 0.039	2.573SerSer: 2.573 ± 0.04	2.548SerThr: 2.548 ± 0.038	3.686SerVal: 3.686 ± 0.042	0.798SerTrp: 0.798 ± 0.017	1.158SerTyr: 1.158 ± 0.022	0.0SerXaa: 0.0 ± 0.0
Thr	6.52ThrAla: 6.52 ± 0.062	0.454ThrCys: 0.454 ± 0.014	2.596ThrAsp: 2.596 ± 0.035	2.177ThrGlu: 2.177 ± 0.028	1.941ThrPhe: 1.941 ± 0.034	4.954ThrGly: 4.954 ± 0.069	1.058ThrHis: 1.058 ± 0.019	2.681ThrIle: 2.681 ± 0.041	1.142ThrLys: 1.142 ± 0.02	5.852ThrLeu: 5.852 ± 0.056	1.227ThrMet: 1.227 ± 0.021	1.071ThrAsn: 1.071 ± 0.025	3.459ThrPro: 3.459 ± 0.038	1.451ThrGln: 1.451 ± 0.028	3.624ThrArg: 3.624 ± 0.038	2.52ThrSer: 2.52 ± 0.034	2.919ThrThr: 2.919 ± 0.043	4.44ThrVal: 4.44 ± 0.055	0.805ThrTrp: 0.805 ± 0.016	1.246ThrTyr: 1.246 ± 0.024	0.0ThrXaa: 0.0 ± 0.0
Val	10.299ValAla: 10.299 ± 0.07	0.73ValCys: 0.73 ± 0.018	4.408ValAsp: 4.408 ± 0.042	4.172ValGlu: 4.172 ± 0.041	2.651ValPhe: 2.651 ± 0.036	6.136ValGly: 6.136 ± 0.061	1.492ValHis: 1.492 ± 0.024	3.754ValIle: 3.754 ± 0.044	2.121ValLys: 2.121 ± 0.032	7.92ValLeu: 7.92 ± 0.066	1.99ValMet: 1.99 ± 0.029	1.886ValAsn: 1.886 ± 0.033	4.422ValPro: 4.422 ± 0.044	2.163ValGln: 2.163 ± 0.031	5.651ValArg: 5.651 ± 0.05	4.016ValSer: 4.016 ± 0.052	4.279ValThr: 4.279 ± 0.048	6.697ValVal: 6.697 ± 0.063	0.973ValTrp: 0.973 ± 0.02	1.441ValTyr: 1.441 ± 0.02	0.0ValXaa: 0.0 ± 0.0
Trp	1.394TrpAla: 1.394 ± 0.025	0.169TrpCys: 0.169 ± 0.008	0.726TrpAsp: 0.726 ± 0.018	0.593TrpGlu: 0.593 ± 0.015	0.565TrpPhe: 0.565 ± 0.014	1.05TrpGly: 1.05 ± 0.021	0.434TrpHis: 0.434 ± 0.011	0.688TrpIle: 0.688 ± 0.017	0.462TrpLys: 0.462 ± 0.016	1.908TrpLeu: 1.908 ± 0.031	0.389TrpMet: 0.389 ± 0.014	0.429TrpAsn: 0.429 ± 0.013	0.865TrpPro: 0.865 ± 0.019	0.645TrpGln: 0.645 ± 0.015	1.513TrpArg: 1.513 ± 0.029	0.876TrpSer: 0.876 ± 0.018	0.89TrpThr: 0.89 ± 0.022	0.896TrpVal: 0.896 ± 0.021	0.285TrpTrp: 0.285 ± 0.013	0.353TrpTyr: 0.353 ± 0.011	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.645TyrAla: 2.645 ± 0.035	0.25TyrCys: 0.25 ± 0.01	1.427TyrAsp: 1.427 ± 0.027	1.101TyrGlu: 1.101 ± 0.022	0.893TyrPhe: 0.893 ± 0.017	2.176TyrGly: 2.176 ± 0.031	0.473TyrHis: 0.473 ± 0.013	0.867TyrIle: 0.867 ± 0.02	0.605TyrLys: 0.605 ± 0.016	2.187TyrLeu: 2.187 ± 0.029	0.451TyrMet: 0.451 ± 0.014	0.52TyrAsn: 0.52 ± 0.016	1.177TyrPro: 1.177 ± 0.024	0.642TyrGln: 0.642 ± 0.017	1.888TyrArg: 1.888 ± 0.024	0.981TyrSer: 0.981 ± 0.019	1.122TyrThr: 1.122 ± 0.022	1.728TyrVal: 1.728 ± 0.026	0.414TyrTrp: 0.414 ± 0.014	0.607TyrTyr: 0.607 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7888 proteins (2550868 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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