Eremomyces bilateralis CBS 781.70

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetes incertae sedis; Eremomycetales; Eremomycetaceae; Eremomyces; Eremomyces bilateralis

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9854 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G1FYL9|A0A6G1FYL9_9PEZI uncharacterized protein OS=Eremomyces bilateralis CBS 781.70 OX=1392243 GN=P152DRAFT_86949 PE=4 SV=1
MM1 pKa = 7.4SRR3 pKa = 11.84PAADD7 pKa = 5.22DD8 pKa = 3.42SWSDD12 pKa = 3.77DD13 pKa = 3.28GDD15 pKa = 3.59ITGTDD20 pKa = 4.06FNNSSTYY27 pKa = 9.52RR28 pKa = 11.84TDD30 pKa = 5.06LNDD33 pKa = 3.6TQDD36 pKa = 3.31TSEE39 pKa = 4.73CDD41 pKa = 3.03SDD43 pKa = 4.15AGFDD47 pKa = 3.86ASPTGPTWLLEE58 pKa = 4.43GNEE61 pKa = 4.65HH62 pKa = 6.23PLEE65 pKa = 4.31YY66 pKa = 10.63YY67 pKa = 10.21KK68 pKa = 10.58QLLKK72 pKa = 11.21NFDD75 pKa = 3.51DD76 pKa = 4.3SEE78 pKa = 4.47YY79 pKa = 10.53TKK81 pKa = 10.67EE82 pKa = 4.49GYY84 pKa = 10.82SNGTTLLLDD93 pKa = 3.57RR94 pKa = 11.84SEE96 pKa = 4.33AQWYY100 pKa = 9.38HH101 pKa = 5.03LTT103 pKa = 3.82

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G1GC72|A0A6G1GC72_9PEZI Eukaryotic translation initiation factor 3 subunit J OS=Eremomyces bilateralis CBS 781.70 OX=1392243 GN=HCR1 PE=3 SV=1
MM1 pKa = 7.42ICTRR5 pKa = 11.84CFPAARR11 pKa = 11.84AILPTRR17 pKa = 11.84PVLNLSSRR25 pKa = 11.84PALLSRR31 pKa = 11.84PNPSQSRR38 pKa = 11.84TLTVLTARR46 pKa = 11.84RR47 pKa = 11.84PLFPSSPSTSTSISTSLSPSPTPFTPLFTSSSTFLGTAPSTHH89 pKa = 7.12PSLATQQLRR98 pKa = 11.84HH99 pKa = 6.02APRR102 pKa = 11.84NTMQRR107 pKa = 11.84WTHH110 pKa = 5.32FVRR113 pKa = 11.84KK114 pKa = 9.01RR115 pKa = 11.84RR116 pKa = 11.84HH117 pKa = 5.26GFLSRR122 pKa = 11.84LRR124 pKa = 11.84TKK126 pKa = 10.65NGRR129 pKa = 11.84KK130 pKa = 6.8TLARR134 pKa = 11.84RR135 pKa = 11.84RR136 pKa = 11.84TKK138 pKa = 10.55GRR140 pKa = 11.84STLSHH145 pKa = 6.65

Molecular weight: 16.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9854

0

9854

4362718

49

4868

442.7

48.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.473 ± 0.022

1.205 ± 0.008

5.642 ± 0.017

6.257 ± 0.026

3.679 ± 0.016

7.144 ± 0.022

2.46 ± 0.011

4.869 ± 0.018

4.768 ± 0.021

8.823 ± 0.027

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.178 ± 0.01

3.496 ± 0.012

6.33 ± 0.032

3.854 ± 0.016

6.475 ± 0.025

8.237 ± 0.03

5.895 ± 0.016

6.115 ± 0.019

1.444 ± 0.01

2.653 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski