Midichloria mitochondrii (strain IricVA)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Candidatus Midichloriaceae; Candidatus Midichloria; Candidatus Midichloria mitochondrii

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F7XVN0|F7XVN0_MIDMI Lipoyl synthase OS=Midichloria mitochondrii (strain IricVA) OX=696127 GN=lipA PE=3 SV=1
MM1 pKa = 7.59INYY4 pKa = 8.02NHH6 pKa = 6.66NPTLSVTQTDD16 pKa = 4.12LEE18 pKa = 4.81DD19 pKa = 3.38NSGIQKK25 pKa = 10.24LYY27 pKa = 10.47PSLTDD32 pKa = 3.56LQEE35 pKa = 3.84NFAYY39 pKa = 10.22EE40 pKa = 4.55NYY42 pKa = 10.78NPMLYY47 pKa = 7.33TTHH50 pKa = 7.11GDD52 pKa = 3.57LEE54 pKa = 4.59TSEE57 pKa = 4.54DD58 pKa = 3.83TYY60 pKa = 11.31EE61 pKa = 4.18APKK64 pKa = 10.34PSAPTEE70 pKa = 4.32SEE72 pKa = 4.14LQPSLACGAPEE83 pKa = 3.74PSAPKK88 pKa = 8.8EE89 pKa = 4.32TEE91 pKa = 3.99PEE93 pKa = 3.83SDD95 pKa = 3.1LAAASVEE102 pKa = 4.54SQFEE106 pKa = 3.89ADD108 pKa = 3.85LQADD112 pKa = 3.6IALEE116 pKa = 3.97EE117 pKa = 4.44SRR119 pKa = 11.84FEE121 pKa = 5.16ADD123 pKa = 2.84MQAAITASLEE133 pKa = 4.31DD134 pKa = 4.2KK135 pKa = 10.51FQADD139 pKa = 3.58IEE141 pKa = 4.56TATAASLKK149 pKa = 9.27KK150 pKa = 8.82TGSSVYY156 pKa = 10.54SYY158 pKa = 11.32DD159 pKa = 3.9PYY161 pKa = 10.91QSCSSAFRR169 pKa = 11.84SEE171 pKa = 4.3DD172 pKa = 3.67YY173 pKa = 10.4FWEE176 pKa = 4.92AGHH179 pKa = 6.82SNLDD183 pKa = 3.76SDD185 pKa = 4.12WVIAA189 pKa = 4.48

Molecular weight: 20.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F7XTZ2|F7XTZ2_MIDMI Flagellar hook-associated protein FlgK putative OS=Midichloria mitochondrii (strain IricVA) OX=696127 GN=flgK PE=4 SV=1
MM1 pKa = 6.51STKK4 pKa = 9.4RR5 pKa = 11.84TYY7 pKa = 10.43QPSKK11 pKa = 9.73LVRR14 pKa = 11.84KK15 pKa = 9.15RR16 pKa = 11.84RR17 pKa = 11.84HH18 pKa = 4.42GFRR21 pKa = 11.84ARR23 pKa = 11.84MATVGGRR30 pKa = 11.84KK31 pKa = 8.92ILNNRR36 pKa = 11.84RR37 pKa = 11.84AQGRR41 pKa = 11.84KK42 pKa = 8.96VLSAA46 pKa = 3.99

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1181

0

1181

280672

37

1582

237.7

26.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.327 ± 0.085

1.129 ± 0.027

4.769 ± 0.058

6.243 ± 0.077

4.703 ± 0.064

6.109 ± 0.059

1.877 ± 0.034

9.125 ± 0.071

7.623 ± 0.069

10.163 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.036

5.427 ± 0.058

3.343 ± 0.049

3.29 ± 0.045

4.1 ± 0.054

7.047 ± 0.066

4.833 ± 0.04

6.274 ± 0.062

0.827 ± 0.024

3.392 ± 0.048
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski